Restriction map for
pFN21AB6650
(Linear) (Six-base) MAPSORT of: KIBB6650.seq Check: 2473 from: 1 to: 1338
KIBB6650.orf
With 182 enzymes: SgfI *
October 7, 2009 14:22 ..
AatII G_ACGT'C
Cuts at: 0 113 1338
Size: 113 1225
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 496 682 1070 1338
Size: 496 186 388 268
Fragments arranged by size:
496 388 268 186
AflIII A'CryG_T
Cuts at: 0 115 1319 1338
Size: 115 1204 19
Fragments arranged by size:
1204 115 19
AlwNI CAG_nnn'CTG
Cuts at: 0 474 1338
Size: 474 864
AseI AT'TA_AT
Cuts at: 0 907 1338
Size: 907 431
AvaI C'yCGr_G
Cuts at: 0 844 1338
Size: 844 494
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 471 504 1338
Size: 471 33 834
Fragments arranged by size:
834 471 33
BanII G_rGCy'C
Cuts at: 0 530 1338
Size: 530 808
BbsI GAAGACnn'nnnn_
Cuts at: 0 196 398 1319 1338
Size: 196 202 921 19
Fragments arranged by size:
921 202 196 19
BciVI GTATCCnnnnn_n'
Cuts at: 0 1156 1338
Size: 1156 182
BclI T'GATC_A
Cuts at: 0 1218 1338
Size: 1218 120
BglII A'GATC_T
Cuts at: 0 1280 1304 1338
Size: 1280 24 34
Fragments arranged by size:
1280 34 24
BlpI GC'TnA_GC
Cuts at: 0 524 1338
Size: 524 814
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 122 481 1338
Size: 122 359 857
Fragments arranged by size:
857 359 122
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 701 1338
Size: 701 637
BsaI GGTCTCn'nnnn_
Cuts at: 0 1 1338
Size: 1 1337
BsaAI yAC'GTr
Cuts at: 0 1320 1338
Size: 1320 18
BsaBI GATnn'nnATC
Cuts at: 0 169 679 1338
Size: 169 510 659
Fragments arranged by size:
659 510 169
BsaHI Gr'CG_yC
Cuts at: 0 110 1338
Size: 110 1228
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 95 125 1338
Size: 95 30 1213
Fragments arranged by size:
1213 95 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 19 559 1338
Size: 19 540 779
Fragments arranged by size:
779 540 19
BseYI C'CCAG_C
Cuts at: 0 134 704 1165 1287 1338
Size: 134 570 461 122 51
Fragments arranged by size:
570 461 134 122 51
BsiHKAI G_wGCw'C
Cuts at: 0 530 1338
Size: 530 808
Bsp1286I G_dGCh'C
Cuts at: 0 530 1338
Size: 530 808
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 299 1338
Size: 299 1039
BsrBI CCG'CTC
Cuts at: 0 1173 1338
Size: 1173 165
BsrDI GCAATG_nn'
Cuts at: 0 893 1338
Size: 893 445
BstYI r'GATC_y
Cuts at: 0 18 1280 1304 1338
Size: 18 1262 24 34
Fragments arranged by size:
1262 34 24 18
EarI CTCTTCn'nnn_
Cuts at: 0 422 1009 1338
Size: 422 587 329
Fragments arranged by size:
587 422 329
EcoICRI GAG'CTC
Cuts at: 0 528 1338
Size: 528 810
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 122 481 496 682 1070 1338
Size: 122 359 15 186 388 268
Fragments arranged by size:
388 359 268 186 122 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 295 1338
Size: 295 1043
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 380 1323 1338
Size: 380 943 15
Fragments arranged by size:
943 380 15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 95 127 1338
Size: 95 32 1211
Fragments arranged by size:
1211 95 32
HincII GTy'rAC
Cuts at: 0 713 1338
Size: 713 625
HindIII A'AGCT_T
Cuts at: 0 281 736 1338
Size: 281 455 602
Fragments arranged by size:
602 455 281
MfeI C'AATT_G
Cuts at: 0 1260 1338
Size: 1260 78
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 560 1338
Size: 560 778
MslI CAynn'nnrTG
Cuts at: 0 214 1338
Size: 214 1124
MspA1I CmG'CkG
Cuts at: 0 34 485 708 780 1338
Size: 34 451 223 72 558
Fragments arranged by size:
558 451 223 72 34
NspI r_CATG'y
Cuts at: 0 119 519 1338
Size: 119 400 819
Fragments arranged by size:
819 400 119
PciI A'CATG_T
Cuts at: 0 115 1338
Size: 115 1223
PflMI CCAn_nnn'nTGG
Cuts at: 0 1141 1338
Size: 1141 197
PfoI T'CCnGG_A
Cuts at: 0 1113 1338
Size: 1113 225
PmlI CAC'GTG
Cuts at: 0 1320 1338
Size: 1320 18
PspXI vC'TCGA_Gb
Cuts at: 0 844 1338
Size: 844 494
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 301 333 1338
Size: 301 32 1005
Fragments arranged by size:
1005 301 32
PstI C_TGCA'G
Cuts at: 0 974 1338
Size: 974 364
PvuII CAG'CTG
Cuts at: 0 34 485 708 780 1338
Size: 34 451 223 72 558
Fragments arranged by size:
558 451 223 72 34
SacI G_AGCT'C
Cuts at: 0 530 1338
Size: 530 808
SfcI C'TryA_G
Cuts at: 0 559 970 1292 1338
Size: 559 411 322 46
Fragments arranged by size:
559 411 322 46
SmlI C'TyrA_G
Cuts at: 0 716 844 1338
Size: 716 128 494
Fragments arranged by size:
716 494 128
StyI C'CwwG_G
Cuts at: 0 296 1226 1338
Size: 296 930 112
Fragments arranged by size:
930 296 112
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 148 1338
Size: 148 1190
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 671 703 1338
Size: 671 32 635
Fragments arranged by size:
671 635 32
XhoI C'TCGA_G
Cuts at: 0 844 1338
Size: 844 494
ZraI GAC'GTC
Cuts at: 0 111 1338
Size: 111 1227
Enzymes that do cut:
AatII AcuI AflIII AlwNI AseI AvaI BaeI BanII
BbsI BciVI BclI BglII BlpI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaXI BseRI BseYI BsiHKAI Bsp1286I
BspMI BsrBI BsrDI BstYI EarI EcoICRI Eco57MI EcoNI
FalI Hin4I HincII HindIII MfeI MmeI MslI MspA1I
NspI PciI PflMI PfoI PmlI PspXI PsrI PstI
PvuII SacI SfcI SmlI StyI TaqII TstI XhoI
ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaI ApaLI ApoI AscI AsiSI
AvrII BamHI BanI BbeI BbvCI BcgI BfrBI BglI
Bme1580I BmgBI BmrI BmtI BplI Bpu10I BsaWI BsgI
BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EciI EcoO109I EcoRI EcoRV FseI FspI
FspAI HaeII HpaI KasI KpnI MluI MscI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PmeI PpiI PpuMI PshAI PsiI PspOMI
PvuI RsrII SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StuI SwaI TatI Tth111I XbaI
XcmI XmaI XmnI