Restriction map for
pFN21AB6648
(Circular) (Six-base) MAPSORT of: pFN21AB6648.seq Check: 7879 from: 1 to: 6040
pFN21AB6648 6040 bp
With 182 enzymes: SgfI *
October 20, 2009 10:57 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3850 278
Size: 53 83 186 936 2314 2468
Fragments arranged by size:
2468 2314 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3383 1937
Size: 1446 4594
Acc65I G'GTAC_C
Cuts at: 3361 3361
Size: 6040
AclI AA'CG_TT
Cuts at: 1563 4167 4540 1563
Size: 2604 373 3063
Fragments arranged by size:
3063 2604 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2187 2396 2948 3263 4103 5487 5755 1632
Size: 555 209 552 315 840 1384 268 1917
Fragments arranged by size:
1917 1384 840 555 552 315 268 209
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5843
AflIII A'CryG_T
Cuts at: 1203 2550 4939 5916 1203
Size: 1347 2389 977 1327
Fragments arranged by size:
2389 1347 1327 977
AgeI A'CCGG_T
Cuts at: 4871 4871
Size: 6040
AhdI GACnn_n'nnGTC
Cuts at: 4769 4769
Size: 6040
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6040
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6040
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3074 3075 3106 3107 3074
Size: 1 31 1 6007
Fragments arranged by size:
6007 31 1 1
AlwNI CAG_nnn'CTG
Cuts at: 2144 3328 3783 5355 2144
Size: 1184 455 1572 2829
Fragments arranged by size:
2829 1572 1184 455
ApaLI G'TGCA_C
Cuts at: 4097 5253 4097
Size: 1156 4884
ApoI r'AATT_y
Cuts at: 1493 2173 2699 2709 2796 2934 3199 3349 3575
Size: 680 526 10 87 138 265 150 226
Cuts at: 3575 3727 5836 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 680 526 265 226 152 150
138 87 10
AseI AT'TA_AT
Cuts at: 160 1032 2639 4594 160
Size: 872 1607 1955 1606
Fragments arranged by size:
1955 1607 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6040
AvaI C'yCGr_G
Cuts at: 1870 1943 2013 3365 1870
Size: 73 70 1352 4545
Fragments arranged by size:
4545 1352 73 70
BamHI G'GATC_C
Cuts at: 3370 3737 3370
Size: 367 5673
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3361 4816 618
Size: 325 217 627 1574 1455 1842
Fragments arranged by size:
1842 1574 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2962 3277 3359 1391
Size: 1571 315 82 4072
Fragments arranged by size:
4072 1571 315 82
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5069
BbvCI CC'TCA_GC
Cuts at: 2019 2019
Size: 6040
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4231 4265 1903
Size: 34 50 34 2210 34 3678
Fragments arranged by size:
3678 2210 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3932 5142 3932
Size: 1210 4830
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6040
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4651 136
Size: 107 122 71 631 605 2979 1525
Fragments arranged by size:
2979 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6035 6035
Size: 6040
BlpI GC'TnA_GC
Cuts at: 3457 3457
Size: 6040
Bme1580I G_kGCm'C
Cuts at: 1790 4101 5257 1790
Size: 2311 1156 2573
Fragments arranged by size:
2573 2311 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6040
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3771 4729 442
Size: 416 53 83 399 24 2354 958 1753
Fragments arranged by size:
2354 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2492 1056
Size: 1436 4604
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4700 1239
Size: 3461 2579
Bpu10I CC'TnA_GC
Cuts at: 2019 2019
Size: 6040
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2822 2873 3261 4165 5030 5328 2822
Size: 51 388 904 865 298 3534
Fragments arranged by size:
3534 904 865 388 298 51
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4703 882
Size: 617 3204 2219
Fragments arranged by size:
3204 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3831 493
Size: 711 2627 2702
Fragments arranged by size:
2702 2627 711
BsaBI GATnn'nnATC
Cuts at: 2775 6034 2775
Size: 3259 2781
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3847 4229 4864 275
Size: 53 83 186 936 2314 382 635 1451
Fragments arranged by size:
2314 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2079 2232 4473 4871 5145 5292 5703 2079
Size: 153 2241 398 274 147 411 2416
Fragments arranged by size:
2416 2241 411 398 274 153 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2074 2104 2114 2144 1178
Size: 30 165 30 671 30 10 30 5074
Fragments arranged by size:
5074 671 165 30 30 30 30 10
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3205 1186
Size: 2019 4021
BseYI C'CCAG_C
Cuts at: 2678 3264 5243 5739 2678
Size: 586 1979 496 2979
Fragments arranged by size:
2979 1979 586 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5467
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2065 3490 4251 5279 1540
Size: 275 186 64 1425 761 1028 2301
Fragments arranged by size:
2301 1425 1028 761 275 186 64
BsiHKAI G_wGCw'C
Cuts at: 2962 4101 4186 5257 2962
Size: 1139 85 1071 3745
Fragments arranged by size:
3745 1139 1071 85
BsmI GAATG_Cn'
Cuts at: 2387 3551 3644 2387
Size: 1164 93 4783
Fragments arranged by size:
4783 1164 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2962 3277 3359 4101 4186 5257 1391
Size: 399 1172 315 82 742 85 1071 2174
Fragments arranged by size:
2174 1172 1071 742 399 315 85 82
BspEI T'CCGG_A
Cuts at: 2079 5703 2079
Size: 3624 2416
BspHI T'CATG_A
Cuts at: 3186 3929 5659 3186
Size: 743 1730 3567
Fragments arranged by size:
3567 1730 743
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3395 844
Size: 2551 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3927 1394
Size: 429 2104 3507
Fragments arranged by size:
3507 2104 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4535 4709 65
Size: 1183 3287 174 1396
Fragments arranged by size:
3287 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4684 4871 1700
Size: 110 2874 187 2869
Fragments arranged by size:
2874 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2037 2250 96
Size: 1941 213 3886
Fragments arranged by size:
3886 1941 213
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6040
BssSI C'ACGA_G
Cuts at: 2512 4100 5112 2512
Size: 1588 1012 3440
Fragments arranged by size:
3440 1588 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6040
BstBI TT'CG_AA
Cuts at: 4844 4844
Size: 6040
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6040
BstYI r'GATC_y
Cuts at: 1972 2076 2281 2425 3324 3370 3737 4122 4139
Size: 104 205 144 899 46 367 385 17
Cuts at: 4139 5591 5677 5689 6035 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 899 385 367 346 205 144
104 86 46 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5488
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5301
Fragments arranged by size:
5301 683 56
BtsI GCAGTG_nn'
Cuts at: 3556 4350 4370 3556
Size: 794 20 5226
Fragments arranged by size:
5226 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6040
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6005
DraI TTT'AAA
Cuts at: 3344 3344
Size: 6040
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5047 809
Size: 507 3731 1802
Fragments arranged by size:
3731 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2062 3487 4376
Size: 54 1425 50 275 18 232 1425 889
Cuts at: 4376 6005 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1425 889 275 232 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2062 3487 1537
Size: 275 250 1425 4090
Fragments arranged by size:
4090 1425 275 250
EarI CTCTTCn'nnn_
Cuts at: 1353 2081 2328 2854 3970 1353
Size: 728 247 526 1116 3423
Fragments arranged by size:
3423 1116 728 526 247
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4627 5001 5147 387
Size: 1064 138 3038 374 146 1280
Fragments arranged by size:
3038 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2960 2960
Size: 6040
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2187 2396 2948 3263 4103 4700 5487
Size: 393 555 209 552 315 840 597 787
Cuts at: 5487 5755 1239
Size: 268 1524
Fragments arranged by size:
1524 840 787 597 555 552 393 315
268 209
EcoO109I rG'GnC_Cy
Cuts at: 3238 3238
Size: 6040
EcoRI G'AATT_C
Cuts at: 2709 3199 3349 3727 2709
Size: 490 150 378 5022
Fragments arranged by size:
5022 490 378 150
EcoRV GAT'ATC
Cuts at: 2599 2599
Size: 6040
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2816 2816
Size: 6040
FspI TGC'GCA
Cuts at: 4546 4546
Size: 6040
HaeII r_GCGC'y
Cuts at: 1678 5187 1678
Size: 3509 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2114 2146 2588 2620 4681
Size: 32 135 32 542 32 442 32 2061
Cuts at: 4681 4713 4755 4787 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2061 542 442 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2364 3384 3630 669
Size: 988 281 426 1020 246 3079
Fragments arranged by size:
3079 1020 988 426 281 246
HindIII A'AGCT_T
Cuts at: 748 2471 748
Size: 1723 4317
HpaI GTT'AAC
Cuts at: 3630 3630
Size: 6040
KpnI G_GTAC'C
Cuts at: 3365 3365
Size: 6040
MfeI C'AATT_G
Cuts at: 3639 3639
Size: 6040
MluI A'CGCG_T
Cuts at: 5916 5916
Size: 6040
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5154 5338 1305
Size: 3849 184 2007
Fragments arranged by size:
3849 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4218
Fragments arranged by size:
4218 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2258 3162 3998 4357 4516 518
Size: 648 1092 904 836 359 159 2042
Fragments arranged by size:
2042 1092 904 836 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2067 3268 3456 4133 5281 5526 1606
Size: 461 1201 188 677 1148 245 2120
Fragments arranged by size:
2120 1201 1148 677 461 245 188
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5488
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6040
NheI G'CTAG_C
Cuts at: 1052 2488 1052
Size: 1436 4604
NotI GC'GGCC_GC
Cuts at: 3487 3487
Size: 6040
NspI r_CATG'y
Cuts at: 1132 1229 2554 2578 3337 3398 4943 1132
Size: 97 1325 24 759 61 1545 2229
Fragments arranged by size:
2229 1545 1325 759 97 61 24
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6040
PciI A'CATG_T
Cuts at: 2550 4939 2550
Size: 2389 3651
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6040
PfoI T'CCnGG_A
Cuts at: 2448 2448
Size: 6040
PmeI GTTT'AAAC
Cuts at: 3344 3344
Size: 6040
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4109 4141 5622 5654 4109
Size: 32 1481 32 4495
Fragments arranged by size:
4495 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3238 3238
Size: 6040
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6040
PsiI TTA'TAA
Cuts at: 3610 3610
Size: 6040
PstI C_TGCA'G
Cuts at: 830 3392 830
Size: 2562 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6040
PvuII CAG'CTG
Cuts at: 3268 3268
Size: 6040
SacI G_AGCT'C
Cuts at: 2962 2962
Size: 6040
SalI G'TCGA_C
Cuts at: 1936 3382 1936
Size: 1446 4594
SbfI CC_TGCA'GG
Cuts at: 3392 3392
Size: 6040
ScaI AGT'ACT
Cuts at: 4288 6000 4288
Size: 1712 4328
SfcI C'TryA_G
Cuts at: 826 1045 3067 3388 4523 5204 5395 826
Size: 219 2022 321 1135 681 191 1471
Fragments arranged by size:
2022 1471 1135 681 321 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6040
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6040
SmaI CCC'GGG
Cuts at: 1872 2015 3367 1872
Size: 143 1352 4545
Fragments arranged by size:
4545 1352 143
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2801 2852 3276 4144 5045 5307
Size: 197 926 858 51 424 868 901 262
Cuts at: 5307 820
Size: 1553
Fragments arranged by size:
1553 926 901 868 858 424 262 197
51
SnaBI TAC'GTA
Cuts at: 493 3831 493
Size: 3338 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6040
SphI G_CATG'C
Cuts at: 1132 3337 3398 1132
Size: 2205 61 3774
Fragments arranged by size:
3774 2205 61
SspI AAT'ATT
Cuts at: 5 52 2267 2499 3964 5
Size: 47 2215 232 1465 2081
Fragments arranged by size:
2215 2081 1465 232 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6040
StyI C'CwwG_G
Cuts at: 513 1065 2310 3479 513
Size: 552 1245 1169 3074
Fragments arranged by size:
3074 1245 1169 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4067 4084 4237 4422 4867 325
Size: 1255 2487 17 153 185 445 1498
Fragments arranged by size:
2487 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2037 2250 4286
Size: 274 80 33 51 1443 60 213 2036
Cuts at: 4286 5998 96
Size: 1712 138
Fragments arranged by size:
2036 1712 1443 274 213 138 80 60
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2074 2106 6000 6032 2074
Size: 32 3894 32 2082
Fragments arranged by size:
3894 2082 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6040
XbaI T'CTAG_A
Cuts at: 3376 3376
Size: 6040
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2446 2446
Size: 6040
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6040
XmaI C'CCGG_G
Cuts at: 1870 2013 3365 1870
Size: 143 1352 4545
Fragments arranged by size:
4545 1352 143
XmnI GAAnn'nnTTC
Cuts at: 2796 4169 2796
Size: 1373 4667
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3848 276
Size: 53 83 186 936 2314 2468
Fragments arranged by size:
2468 2314 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SacI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI