(Circular) (Six-base) MAPSORT of: pFN21AB6639.seq Check: 1131 from: 1 to: 5959 pFN21AB6639 5959 bp With 182 enzymes: SgfI * October 20, 2009 10:57 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2944 3004 3769 278 Size: 53 83 186 936 1408 60 765 2468 Fragments arranged by size: 2468 1408 936 765 186 83 60 53 AccI GT'mk_AC Cuts at: 1937 2051 2771 3302 1937 Size: 114 720 531 4594 Fragments arranged by size: 4594 720 531 114 Acc65I G'GTAC_C Cuts at: 3280 3280 Size: 5959 AclI AA'CG_TT Cuts at: 1563 4086 4459 1563 Size: 2523 373 3063 Fragments arranged by size: 3063 2523 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2340 2568 4022 5406 5674 1632 Size: 708 228 1454 1384 268 1917 Fragments arranged by size: 1917 1454 1384 708 268 228 AfeI AGC'GCT Cuts at: 2693 2693 Size: 5959 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5762 AflIII A'CryG_T Cuts at: 1203 4858 5835 1203 Size: 3655 977 1327 Fragments arranged by size: 3655 1327 977 AgeI A'CCGG_T Cuts at: 2495 4790 2495 Size: 2295 3664 AhdI GACnn_n'nnGTC Cuts at: 4688 4688 Size: 5959 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5959 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3144 1184 Size: 1960 3999 AlwNI CAG_nnn'CTG Cuts at: 2318 2623 3702 5274 2318 Size: 305 1079 1572 3003 Fragments arranged by size: 3003 1572 1079 305 ApaLI G'TGCA_C Cuts at: 4016 5172 4016 Size: 1156 4803 ApoI r'AATT_y Cuts at: 1493 2726 3268 3494 3646 5755 1493 Size: 1233 542 226 152 2109 1697 Fragments arranged by size: 2109 1697 1233 542 226 152 AseI AT'TA_AT Cuts at: 160 1032 4513 160 Size: 872 3481 1606 Fragments arranged by size: 3481 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5959 AvaI C'yCGr_G Cuts at: 1870 1943 3284 1870 Size: 73 1341 4545 Fragments arranged by size: 4545 1341 73 BamHI G'GATC_C Cuts at: 3289 3656 3289 Size: 367 5592 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3280 4735 618 Size: 325 217 627 1493 1455 1842 Fragments arranged by size: 1842 1493 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3278 1391 Size: 1887 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2328 928 Size: 971 429 4559 Fragments arranged by size: 4559 971 429 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4150 4184 1903 Size: 34 2213 34 3678 Fragments arranged by size: 3678 2213 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3851 5061 3851 Size: 1210 4749 BclI T'GATC_A Cuts at: 1548 2137 1548 Size: 589 5370 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4570 136 Size: 107 122 71 631 605 2898 1525 Fragments arranged by size: 2898 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5954 5954 Size: 5959 BlpI GC'TnA_GC Cuts at: 3376 3376 Size: 5959 Bme1580I G_kGCm'C Cuts at: 1790 4020 5176 1790 Size: 2230 1156 2573 Fragments arranged by size: 2573 2230 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5959 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3690 4648 442 Size: 416 53 83 399 24 2273 958 1753 Fragments arranged by size: 2273 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5959 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2346 2637 2991 4619 1239 Size: 1107 291 354 1628 2579 Fragments arranged by size: 2579 1628 1107 354 291 Bpu10I CC'TnA_GC Cuts at: 2449 2676 2449 Size: 227 5732 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2452 4084 4949 5247 2452 Size: 1632 865 298 3164 Fragments arranged by size: 3164 1632 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2625 4622 882 Size: 617 1126 1997 2219 Fragments arranged by size: 2219 1997 1126 617 BsaAI yAC'GTr Cuts at: 493 1204 3750 493 Size: 711 2546 2702 Fragments arranged by size: 2702 2546 711 BsaBI GATnn'nnATC Cuts at: 5953 5953 Size: 5959 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2941 3001 3766 4148 Size: 53 83 186 936 1408 60 765 382 Cuts at: 4148 4783 275 Size: 635 1451 Fragments arranged by size: 1451 1408 936 765 635 382 186 83 60 53 BsaWI w'CCGG_w Cuts at: 2495 4392 4790 5064 5211 5622 2495 Size: 1897 398 274 147 411 2832 Fragments arranged by size: 2832 1897 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2332 2362 2508 2538 1178 Size: 30 165 30 929 30 146 30 4599 Fragments arranged by size: 4599 929 165 146 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5959 BseYI C'CCAG_C Cuts at: 5162 5658 5162 Size: 496 5463 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2453 1163 Size: 573 717 4669 Fragments arranged by size: 4669 717 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3409 4170 5198 1540 Size: 275 186 1408 761 1028 2301 Fragments arranged by size: 2301 1408 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4020 4105 5176 4020 Size: 85 1071 4803 Fragments arranged by size: 4803 1071 85 BsmI GAATG_Cn' Cuts at: 3470 3563 3470 Size: 93 5866 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3278 4020 4105 5176 1391 Size: 399 1488 742 85 1071 2174 Fragments arranged by size: 2174 1488 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5622 5622 Size: 5959 BspHI T'CATG_A Cuts at: 3848 5578 3848 Size: 1730 4229 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3314 844 Size: 2470 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3846 1394 Size: 429 2023 3507 Fragments arranged by size: 3507 2023 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2424 4454 4628 65 Size: 1183 1176 2030 174 1396 Fragments arranged by size: 2030 1396 1183 1176 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2020 2495 4603 4790 1700 Size: 110 210 475 2108 187 2869 Fragments arranged by size: 2869 2108 475 210 187 110 BsrGI T'GTAC_A Cuts at: 96 2421 3097 96 Size: 2325 676 2958 Fragments arranged by size: 2958 2325 676 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5959 BssSI C'ACGA_G Cuts at: 4019 5031 4019 Size: 1012 4947 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5959 BstBI TT'CG_AA Cuts at: 4763 4763 Size: 5959 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5959 BstYI r'GATC_y Cuts at: 1972 3289 3656 4041 4058 5510 5596 5608 5954 Size: 1317 367 385 17 1452 86 12 346 Cuts at: 5954 1972 Size: 1977 Fragments arranged by size: 1977 1452 1317 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2052 2772 2052 Size: 720 5239 BtgI C'CryG_G Cuts at: 513 1065 2004 2242 513 Size: 552 939 238 4230 Fragments arranged by size: 4230 939 552 238 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5220 Fragments arranged by size: 5220 683 56 BtsI GCAGTG_nn' Cuts at: 3475 4269 4289 3475 Size: 794 20 5145 Fragments arranged by size: 5145 794 20 ClaI AT'CG_AT Cuts at: 1554 2811 1554 Size: 1257 4702 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5924 DraI TTT'AAA Cuts at: 2299 3263 2299 Size: 964 4995 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4966 809 Size: 507 3650 1802 Fragments arranged by size: 3650 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2231 3406 4295 Size: 54 1425 50 275 18 401 1175 889 Cuts at: 4295 5924 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1175 889 401 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3406 1537 Size: 275 1594 4090 Fragments arranged by size: 4090 1594 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3889 1353 Size: 2536 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2228 4546 4920 5066 387 Size: 1064 138 639 2318 374 146 1280 Fragments arranged by size: 2318 1280 1064 639 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2340 2346 2568 2637 2991 4022 4619 Size: 393 708 6 222 69 354 1031 597 Cuts at: 4619 5406 5674 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1031 787 708 597 393 354 268 222 69 6 EcoO109I rG'GnC_Cy Cuts at: 2837 2837 Size: 5959 EcoRI G'AATT_C Cuts at: 3268 3646 3268 Size: 378 5581 FspI TGC'GCA Cuts at: 4465 4465 Size: 5959 HaeII r_GCGC'y Cuts at: 1678 2695 5106 1678 Size: 1017 2411 2531 Fragments arranged by size: 2531 2411 1017 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2332 2364 2508 2540 2829 Size: 32 135 32 760 32 144 32 289 Cuts at: 2829 2861 2931 2963 4600 4632 4674 4706 1373 Size: 32 70 32 1637 32 42 32 2626 Fragments arranged by size: 2626 1637 760 289 144 135 70 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2493 3303 3549 669 Size: 988 281 555 810 246 3079 Fragments arranged by size: 3079 988 810 555 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5959 HpaI GTT'AAC Cuts at: 3549 3549 Size: 5959 KpnI G_GTAC'C Cuts at: 3284 3284 Size: 5959 MfeI C'AATT_G Cuts at: 3558 3558 Size: 5959 MluI A'CGCG_T Cuts at: 5835 5835 Size: 5959 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5073 5257 1305 Size: 3768 184 2007 Fragments arranged by size: 3768 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2233 10 Size: 54 1425 343 401 3736 Fragments arranged by size: 3736 1425 401 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2429 2540 3917 4276 4435 518 Size: 648 1263 111 1377 359 159 2042 Fragments arranged by size: 2042 1377 1263 648 359 159 111 MspA1I CmG'CkG Cuts at: 1606 2568 3375 4052 5200 5445 1606 Size: 962 807 677 1148 245 2120 Fragments arranged by size: 2120 1148 962 807 677 245 NaeI GCC'GGC Cuts at: 2022 2022 Size: 5959 NcoI C'CATG_G Cuts at: 513 1065 2004 2242 513 Size: 552 939 238 4230 Fragments arranged by size: 4230 939 552 238 NdeI CA'TA_TG Cuts at: 387 2218 387 Size: 1831 4128 NgoMIV G'CCGG_C Cuts at: 2020 2020 Size: 5959 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5959 NotI GC'GGCC_GC Cuts at: 3406 3406 Size: 5959 NspI r_CATG'y Cuts at: 1132 1229 3121 3317 4862 1132 Size: 97 1892 196 1545 2229 Fragments arranged by size: 2229 1892 1545 196 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5959 PciI A'CATG_T Cuts at: 4858 4858 Size: 5959 PflMI CCAn_nnn'nTGG Cuts at: 1108 3245 1108 Size: 2137 3822 PmeI GTTT'AAAC Cuts at: 3263 3263 Size: 5959 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4028 4060 5541 5573 4028 Size: 32 1481 32 4414 Fragments arranged by size: 4414 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2837 2837 Size: 5959 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5959 PsiI TTA'TAA Cuts at: 3529 3529 Size: 5959 PstI C_TGCA'G Cuts at: 830 3311 830 Size: 2481 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5959 PvuII CAG'CTG Cuts at: 2568 2568 Size: 5959 SalI G'TCGA_C Cuts at: 1936 3301 1936 Size: 1365 4594 SbfI CC_TGCA'GG Cuts at: 3311 3311 Size: 5959 ScaI AGT'ACT Cuts at: 4207 5919 4207 Size: 1712 4247 SfcI C'TryA_G Cuts at: 826 1045 2695 3030 3307 4442 5123 5314 826 Size: 219 1650 335 277 1135 681 191 1471 Fragments arranged by size: 1650 1471 1135 681 335 277 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5959 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5959 SmaI CCC'GGG Cuts at: 1872 3286 1872 Size: 1414 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2467 4063 4964 5226 820 Size: 197 926 524 1596 901 262 1553 Fragments arranged by size: 1596 1553 926 901 524 262 197 SnaBI TAC'GTA Cuts at: 493 3750 493 Size: 3257 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5959 SphI G_CATG'C Cuts at: 1132 3317 1132 Size: 2185 3774 SspI AAT'ATT Cuts at: 5 52 2303 2959 3883 5 Size: 47 2251 656 924 2081 Fragments arranged by size: 2251 2081 924 656 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5959 StyI C'CwwG_G Cuts at: 513 1065 2004 2242 2664 3398 513 Size: 552 939 238 422 734 3074 Fragments arranged by size: 3074 939 734 552 422 238 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2123 2649 3986 4003 4156 4341 4786 Size: 1255 543 526 1337 17 153 185 445 Cuts at: 4786 325 Size: 1498 Fragments arranged by size: 1498 1337 1255 543 526 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2421 3097 4205 Size: 274 80 33 51 1443 444 676 1108 Cuts at: 4205 5917 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1108 676 444 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5919 5951 5919 Size: 32 5927 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5959 XbaI T'CTAG_A Cuts at: 3295 3295 Size: 5959 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5959 XmaI C'CCGG_G Cuts at: 1870 3284 1870 Size: 1414 4545 XmnI GAAnn'nnTTC Cuts at: 2913 4088 2913 Size: 1175 4784 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2942 3002 3767 276 Size: 53 83 186 936 1408 60 765 2468 Fragments arranged by size: 2468 1408 936 765 186 83 60 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI