Restriction map for
pFN21AB6635
(Circular) (Six-base) MAPSORT of: pFN21AB6635.seq Check: 7518 from: 1 to: 5959
pFN21AB6635 5959 bp
With 182 enzymes: SgfI *
October 20, 2009 10:57 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2128 2128
Size: 5959
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3769 278
Size: 53 83 186 936 2233 2468
Fragments arranged by size:
2468 2233 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3302 1937
Size: 1365 4594
Acc65I G'GTAC_C
Cuts at: 3280 3280
Size: 5959
AclI AA'CG_TT
Cuts at: 1563 4086 4459 1563
Size: 2523 373 3063
Fragments arranged by size:
3063 2523 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3215 4022 5406 5674 1632
Size: 1583 807 1384 268 1917
Fragments arranged by size:
1917 1583 1384 807 268
AflII C'TTAA_G
Cuts at: 820 1017 2938 820
Size: 197 1921 3841
Fragments arranged by size:
3841 1921 197
AflIII A'CryG_T
Cuts at: 1203 4858 5835 1203
Size: 3655 977 1327
Fragments arranged by size:
3655 1327 977
AgeI A'CCGG_T
Cuts at: 4790 4790
Size: 5959
AhdI GACnn_n'nnGTC
Cuts at: 4688 4688
Size: 5959
AleI CACnn'nnGTG
Cuts at: 1166 2757 1166
Size: 1591 4368
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5959
AlwNI CAG_nnn'CTG
Cuts at: 2425 3223 3702 5274 2425
Size: 798 479 1572 3110
Fragments arranged by size:
3110 1572 798 479
ApaLI G'TGCA_C
Cuts at: 4016 5172 4016
Size: 1156 4803
ApoI r'AATT_y
Cuts at: 1493 2180 2212 2255 2402 2524 3089 3166 3268
Size: 687 32 43 147 122 565 77 102
Cuts at: 3268 3494 3646 5755 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 687 565 226 152 147 122
102 77 43 32
AseI AT'TA_AT
Cuts at: 160 1032 4513 160
Size: 872 3481 1606
Fragments arranged by size:
3481 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5959
AvaI C'yCGr_G
Cuts at: 1870 1943 3284 1870
Size: 73 1341 4545
Fragments arranged by size:
4545 1341 73
BamHI G'GATC_C
Cuts at: 3289 3656 3289
Size: 367 5592
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3280 4735 618
Size: 325 217 627 1493 1455 1842
Fragments arranged by size:
1842 1493 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3278 1391
Size: 1887 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2298 2462 2527 2601 2910 928
Size: 971 399 164 65 74 309 3977
Fragments arranged by size:
3977 971 399 309 164 74 65
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4150 4184 1903
Size: 34 50 34 2129 34 3678
Fragments arranged by size:
3678 2129 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3851 5061 3851
Size: 1210 4749
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5959
BfrBI ATG'CAT
Cuts at: 2470 2720 2470
Size: 250 5709
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4570 136
Size: 107 122 71 631 605 2898 1525
Fragments arranged by size:
2898 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2113 5954 2113
Size: 3841 2118
BlpI GC'TnA_GC
Cuts at: 3376 3376
Size: 5959
Bme1580I G_kGCm'C
Cuts at: 1790 4020 5176 1790
Size: 2230 1156 2573
Fragments arranged by size:
2573 2230 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5959
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3690 4648 442
Size: 416 53 83 399 24 2273 958 1753
Fragments arranged by size:
2273 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5959
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4619 1239
Size: 3380 2579
Bpu10I CC'TnA_GC
Cuts at: 2958 2958
Size: 5959
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2934 4084 4949 5247 2934
Size: 1150 865 298 3646
Fragments arranged by size:
3646 1150 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4622 882
Size: 617 3123 2219
Fragments arranged by size:
3123 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3750 493
Size: 711 2546 2702
Fragments arranged by size:
2702 2546 711
BsaBI GATnn'nnATC
Cuts at: 5953 5953
Size: 5959
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3766 4148 4783 275
Size: 53 83 186 936 2233 382 635 1451
Fragments arranged by size:
2233 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4392 4790 5064 5211 5622 4392
Size: 398 274 147 411 4729
Fragments arranged by size:
4729 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2622 2652 3243 3273 1178
Size: 30 165 30 1219 30 591 30 3864
Fragments arranged by size:
3864 1219 591 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5959
BseYI C'CCAG_C
Cuts at: 5162 5658 5162
Size: 496 5463
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5386
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3409 4170 5198 1540
Size: 275 186 1408 761 1028 2301
Fragments arranged by size:
2301 1408 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2676 4020 4105 5176 2676
Size: 1344 85 1071 3459
Fragments arranged by size:
3459 1344 1071 85
BsmI GAATG_Cn'
Cuts at: 3155 3470 3563 3155
Size: 315 93 5551
Fragments arranged by size:
5551 315 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2676 3278 4020 4105 5176 1391
Size: 399 886 602 742 85 1071 2174
Fragments arranged by size:
2174 1071 886 742 602 399 85
BspEI T'CCGG_A
Cuts at: 5622 5622
Size: 5959
BspHI T'CATG_A
Cuts at: 2269 3848 5578 2269
Size: 1579 1730 2650
Fragments arranged by size:
2650 1730 1579
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2128 2291 3314 844
Size: 1284 163 1023 3489
Fragments arranged by size:
3489 1284 1023 163
BsrBI CCG'CTC
Cuts at: 1394 1823 2167 3846 1394
Size: 429 344 1679 3507
Fragments arranged by size:
3507 1679 429 344
BsrDI GCAATG_nn'
Cuts at: 65 1248 2204 4454 4628 65
Size: 1183 956 2250 174 1396
Fragments arranged by size:
2250 1396 1183 956 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2018 4603 4790 1700
Size: 110 208 2585 187 2869
Fragments arranged by size:
2869 2585 208 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5959
BssHII G'CGCG_C
Cuts at: 1925 2008 1925
Size: 83 5876
BssSI C'ACGA_G
Cuts at: 2886 4019 5031 2886
Size: 1133 1012 3814
Fragments arranged by size:
3814 1133 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5959
BstBI TT'CG_AA
Cuts at: 4763 4763
Size: 5959
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5959
BstYI r'GATC_y
Cuts at: 1972 2113 2747 2962 3289 3656 4041 4058 5510
Size: 141 634 215 327 367 385 17 1452
Cuts at: 5510 5596 5608 5954 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 634 385 367 346 327 215
141 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2089 513
Size: 552 1024 4383
Fragments arranged by size:
4383 1024 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2480 487
Size: 683 56 1254 3966
Fragments arranged by size:
3966 1254 683 56
BtsI GCAGTG_nn'
Cuts at: 3246 3475 4269 4289 3246
Size: 229 794 20 4916
Fragments arranged by size:
4916 794 229 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5959
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5924
DraI TTT'AAA
Cuts at: 2218 2523 2605 2928 3263 2218
Size: 305 82 323 335 4914
Fragments arranged by size:
4914 335 323 305 82
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4966 809
Size: 507 3650 1802
Fragments arranged by size:
3650 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3406 4295 5924
Size: 54 1425 50 275 18 1576 889 1629
Cuts at: 5924 8
Size: 43
Fragments arranged by size:
1629 1576 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3406 1537
Size: 275 1594 4090
Fragments arranged by size:
4090 1594 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2076 2509 3139 3889 1353
Size: 723 433 630 750 3423
Fragments arranged by size:
3423 750 723 630 433
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4546 4920 5066 387
Size: 1064 138 2957 374 146 1280
Fragments arranged by size:
2957 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3215 4022 4619 5406 5674 1239
Size: 393 1583 807 597 787 268 1524
Fragments arranged by size:
1583 1524 807 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2402 3268 3646 2402
Size: 866 378 4715
Fragments arranged by size:
4715 866 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2606 2606
Size: 5959
FspI TGC'GCA
Cuts at: 4465 4465
Size: 5959
HaeII r_GCGC'y
Cuts at: 1678 5106 1678
Size: 3428 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2060 2092 4600 4632 4674
Size: 32 135 32 488 32 2508 32 42
Cuts at: 4674 4706 1373
Size: 32 2626
Fragments arranged by size:
2626 2508 488 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3303 3549 669
Size: 988 281 1365 246 3079
Fragments arranged by size:
3079 1365 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2366 748
Size: 1618 4341
HpaI GTT'AAC
Cuts at: 3549 3549
Size: 5959
KpnI G_GTAC'C
Cuts at: 3284 3284
Size: 5959
MfeI C'AATT_G
Cuts at: 3558 3558
Size: 5959
MluI A'CGCG_T
Cuts at: 5835 5835
Size: 5959
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5073 5257 1305
Size: 3768 184 2007
Fragments arranged by size:
3768 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4137
Fragments arranged by size:
4137 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2411 2757 3917 4276 4435 518
Size: 648 1245 346 1160 359 159 2042
Fragments arranged by size:
2042 1245 1160 648 359 346 159
MspA1I CmG'CkG
Cuts at: 1606 2091 3375 4052 5200 5445 1606
Size: 485 1284 677 1148 245 2120
Fragments arranged by size:
2120 1284 1148 677 485 245
NaeI GCC'GGC
Cuts at: 2020 2020
Size: 5959
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5407
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5959
NgoMIV G'CCGG_C
Cuts at: 2018 2018
Size: 5959
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5959
NotI GC'GGCC_GC
Cuts at: 3406 3406
Size: 5959
NsiI A_TGCA'T
Cuts at: 2472 2722 2472
Size: 250 5709
NspI r_CATG'y
Cuts at: 1132 1229 3203 3317 4862 1132
Size: 97 1974 114 1545 2229
Fragments arranged by size:
2229 1974 1545 114 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5959
PciI A'CATG_T
Cuts at: 4858 4858
Size: 5959
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5959
PmeI GTTT'AAAC
Cuts at: 3263 3263
Size: 5959
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4028 4060 5541 5573 4028
Size: 32 1481 32 4414
Fragments arranged by size:
4414 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5959
PsiI TTA'TAA
Cuts at: 3529 3529
Size: 5959
PstI C_TGCA'G
Cuts at: 830 2139 2767 3228 3311 830
Size: 1309 628 461 83 3478
Fragments arranged by size:
3478 1309 628 461 83
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5959
SacII CC_GC'GG
Cuts at: 2092 2092
Size: 5959
SalI G'TCGA_C
Cuts at: 1936 3301 1936
Size: 1365 4594
SbfI CC_TGCA'GG
Cuts at: 3311 3311
Size: 5959
ScaI AGT'ACT
Cuts at: 4207 5919 4207
Size: 1712 4247
SfcI C'TryA_G
Cuts at: 826 1045 2135 2360 2763 2820 3053 3215 3224
Size: 219 1090 225 403 57 233 162 9
Cuts at: 3224 3307 4442 5123 5314 826
Size: 83 1135 681 191 1471
Fragments arranged by size:
1471 1135 1090 681 403 233 225 219
191 162 83 57 9
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5959
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5959
SmaI CCC'GGG
Cuts at: 1872 3286 1872
Size: 1414 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2938 2949 4063 4964 5226 820
Size: 197 926 995 11 1114 901 262 1553
Fragments arranged by size:
1553 1114 995 926 901 262 197 11
SnaBI TAC'GTA
Cuts at: 493 3750 493
Size: 3257 2702
SpeI A'CTAG_T
Cuts at: 152 2989 152
Size: 2837 3122
SphI G_CATG'C
Cuts at: 1132 3203 3317 1132
Size: 2071 114 3774
Fragments arranged by size:
3774 2071 114
SspI AAT'ATT
Cuts at: 5 52 2438 2448 2933 3883 5
Size: 47 2386 10 485 950 2081
Fragments arranged by size:
2386 2081 950 485 47 10
StuI AGG'CCT
Cuts at: 1517 2052 1517
Size: 535 5424
StyI C'CwwG_G
Cuts at: 513 1065 3398 513
Size: 552 2333 3074
Fragments arranged by size:
3074 2333 552
SwaI ATTT'AAAT
Cuts at: 2523 2523
Size: 5959
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3986 4003 4156 4341 4786 325
Size: 1255 2406 17 153 185 445 1498
Fragments arranged by size:
2406 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4205 5917 96
Size: 274 80 33 51 1443 2228 1712 138
Fragments arranged by size:
2228 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5919 5951 5919
Size: 32 5927
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5959
XbaI T'CTAG_A
Cuts at: 3295 3295
Size: 5959
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5959
XmaI C'CCGG_G
Cuts at: 1870 3284 1870
Size: 1414 4545
XmnI GAAnn'nnTTC
Cuts at: 2785 4088 2785
Size: 1303 4656
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3767 276
Size: 53 83 186 936 2233 2468
Fragments arranged by size:
2468 2233 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PstI PvuI SacII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI SwaI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoO109I EcoRV FseI FspAI KasI NarI NruI
PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII
RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI
XcmI