(Circular) (Six-base) MAPSORT of: pFN21AB6627.seq Check: 4713 from: 1 to: 5950 pFN21AB6627 5950 bp With 182 enzymes: SgfI * October 20, 2009 10:57 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3760 278 Size: 53 83 186 936 2224 2468 Fragments arranged by size: 2468 2224 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2130 2472 2610 2925 3293 1937 Size: 193 342 138 315 368 4594 Fragments arranged by size: 4594 368 342 315 193 138 Acc65I G'GTAC_C Cuts at: 3271 3271 Size: 5950 AclI AA'CG_TT Cuts at: 1563 4077 4450 1563 Size: 2514 373 3063 Fragments arranged by size: 3063 2514 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2162 2583 2928 3081 3126 4013 5397 5665 Size: 530 421 345 153 45 887 1384 268 Cuts at: 5665 1632 Size: 1917 Fragments arranged by size: 1917 1384 887 530 421 345 268 153 45 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5753 AflIII A'CryG_T Cuts at: 1203 4849 5826 1203 Size: 3646 977 1327 Fragments arranged by size: 3646 1327 977 AgeI A'CCGG_T Cuts at: 4781 4781 Size: 5950 AhdI GACnn_n'nnGTC Cuts at: 4679 4679 Size: 5950 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5950 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5950 AlwNI CAG_nnn'CTG Cuts at: 3116 3693 5265 3116 Size: 577 1572 3801 Fragments arranged by size: 3801 1572 577 ApaLI G'TGCA_C Cuts at: 4007 5163 4007 Size: 1156 4794 ApoI r'AATT_y Cuts at: 1493 3259 3485 3637 5746 1493 Size: 1766 226 152 2109 1697 Fragments arranged by size: 2109 1766 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 2511 4504 160 Size: 872 1479 1993 1606 Fragments arranged by size: 1993 1606 1479 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5950 AvaI C'yCGr_G Cuts at: 1870 1943 3275 1870 Size: 73 1332 4545 Fragments arranged by size: 4545 1332 73 BamHI G'GATC_C Cuts at: 3280 3647 3280 Size: 367 5583 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3271 4726 618 Size: 325 217 627 1484 1455 1842 Fragments arranged by size: 1842 1484 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2141 3269 1391 Size: 750 1128 4072 Fragments arranged by size: 4072 1128 750 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2163 2751 928 Size: 971 264 588 4127 Fragments arranged by size: 4127 971 588 264 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4141 4175 1903 Size: 34 2204 34 3678 Fragments arranged by size: 3678 2204 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2575 2729 3842 5052 2575 Size: 154 1113 1210 3473 Fragments arranged by size: 3473 1210 1113 154 BclI T'GATC_A Cuts at: 1548 1548 Size: 5950 BfrBI ATG'CAT Cuts at: 2351 3202 2351 Size: 851 5099 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4561 136 Size: 107 122 71 631 605 2889 1525 Fragments arranged by size: 2889 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5945 5945 Size: 5950 BlpI GC'TnA_GC Cuts at: 2685 3367 2685 Size: 682 5268 Bme1580I G_kGCm'C Cuts at: 1790 3104 4011 5167 1790 Size: 1314 907 1156 2573 Fragments arranged by size: 2573 1314 1156 907 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5950 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2929 3681 4639 Size: 416 53 83 399 24 1512 752 958 Cuts at: 4639 442 Size: 1753 Fragments arranged by size: 1753 1512 958 752 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5950 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2125 2205 2802 4610 1239 Size: 886 80 597 1808 2579 Fragments arranged by size: 2579 1808 886 597 80 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4075 4940 5238 4075 Size: 865 298 4787 Fragments arranged by size: 4787 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4613 882 Size: 617 3114 2219 Fragments arranged by size: 3114 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3741 493 Size: 711 2537 2702 Fragments arranged by size: 2702 2537 711 BsaBI GATnn'nnATC Cuts at: 5944 5944 Size: 5950 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2997 3757 4139 4774 Size: 53 83 186 936 1464 760 382 635 Cuts at: 4774 275 Size: 1451 Fragments arranged by size: 1464 1451 936 760 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4383 4781 5055 5202 5613 4383 Size: 398 274 147 411 4720 Fragments arranged by size: 4720 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2123 2153 1178 Size: 30 165 30 720 30 4975 Fragments arranged by size: 4975 720 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2443 3110 1186 Size: 1257 667 4026 Fragments arranged by size: 4026 1257 667 BseYI C'CCAG_C Cuts at: 5153 5649 5153 Size: 496 5454 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2508 1163 Size: 573 772 4605 Fragments arranged by size: 4605 772 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3400 4161 5189 1540 Size: 275 186 1399 761 1028 2301 Fragments arranged by size: 2301 1399 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4011 4096 5167 4011 Size: 85 1071 4794 Fragments arranged by size: 4794 1071 85 BsmI GAATG_Cn' Cuts at: 2608 3461 3554 2608 Size: 853 93 5004 Fragments arranged by size: 5004 853 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2141 3104 3269 4011 4096 5167 1391 Size: 399 351 963 165 742 85 1071 2174 Fragments arranged by size: 2174 1071 963 742 399 351 165 85 BspEI T'CCGG_A Cuts at: 5613 5613 Size: 5950 BspHI T'CATG_A Cuts at: 3839 5569 3839 Size: 1730 4220 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3305 844 Size: 2461 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2328 3837 1394 Size: 429 505 1509 3507 Fragments arranged by size: 3507 1509 505 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4445 4619 65 Size: 1183 3197 174 1396 Fragments arranged by size: 3197 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4594 4781 1700 Size: 110 2784 187 2869 Fragments arranged by size: 2869 2784 187 110 BsrGI T'GTAC_A Cuts at: 96 2698 96 Size: 2602 3348 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5950 BssSI C'ACGA_G Cuts at: 4010 5022 4010 Size: 1012 4938 BstAPI GCAn_nnn'nTGC Cuts at: 794 2300 2348 794 Size: 1506 48 4396 Fragments arranged by size: 4396 1506 48 BstBI TT'CG_AA Cuts at: 4754 4754 Size: 5950 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5950 BstXI CCAn_nnnn'nTGG Cuts at: 2125 2839 2125 Size: 714 5236 BstYI r'GATC_y Cuts at: 1972 2011 2256 2966 3280 3647 4032 4049 5501 Size: 39 245 710 314 367 385 17 1452 Cuts at: 5501 5587 5599 5945 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 710 385 367 346 314 245 86 39 17 12 BstZ17I GTA'TAC Cuts at: 2926 2926 Size: 5950 BtgI C'CryG_G Cuts at: 513 1065 2004 2099 2118 2124 3078 513 Size: 552 939 95 19 6 954 3385 Fragments arranged by size: 3385 954 939 552 95 19 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5211 Fragments arranged by size: 5211 683 56 BtsI GCAGTG_nn' Cuts at: 3466 4260 4280 3466 Size: 794 20 5136 Fragments arranged by size: 5136 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5950 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5915 DraI TTT'AAA Cuts at: 3254 3254 Size: 5950 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4957 809 Size: 507 3641 1802 Fragments arranged by size: 3641 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2121 2166 3397 Size: 54 1425 50 275 18 291 45 1231 Cuts at: 3397 4286 5915 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1231 889 291 275 54 50 45 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3397 1537 Size: 275 1585 4090 Fragments arranged by size: 4090 1585 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2093 2943 3880 1353 Size: 740 850 937 3423 Fragments arranged by size: 3423 937 850 740 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4537 4911 5057 387 Size: 1064 138 2948 374 146 1280 Fragments arranged by size: 2948 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2125 2162 2205 2583 2802 2928 3081 Size: 393 493 37 43 378 219 126 153 Cuts at: 3081 3126 4013 4610 5397 5665 1239 Size: 45 887 597 787 268 1524 Fragments arranged by size: 1524 887 787 597 493 393 378 268 219 153 126 45 43 37 EcoRI G'AATT_C Cuts at: 3259 3637 3259 Size: 378 5572 FspI TGC'GCA Cuts at: 4456 4456 Size: 5950 HaeII r_GCGC'y Cuts at: 1678 5097 1678 Size: 3419 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4591 4623 4665 4697 1373 Size: 32 135 32 3019 32 42 32 2626 Fragments arranged by size: 3019 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3294 3540 669 Size: 988 281 1356 246 3079 Fragments arranged by size: 3079 1356 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5950 HpaI GTT'AAC Cuts at: 3540 3540 Size: 5950 KpnI G_GTAC'C Cuts at: 3275 3275 Size: 5950 MfeI C'AATT_G Cuts at: 3549 3549 Size: 5950 MluI A'CGCG_T Cuts at: 5826 5826 Size: 5950 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3015 5064 5248 1305 Size: 1710 2049 184 2007 Fragments arranged by size: 2049 2007 1710 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2123 2168 10 Size: 54 1425 343 291 45 3792 Fragments arranged by size: 3792 1425 343 291 54 45 MslI CAynn'nnrTG Cuts at: 518 1166 2123 2987 3908 4267 4426 518 Size: 648 957 864 921 359 159 2042 Fragments arranged by size: 2042 957 921 864 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2238 3116 3366 4043 5191 5436 1606 Size: 632 878 250 677 1148 245 2120 Fragments arranged by size: 2120 1148 878 677 632 250 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2099 2118 2124 3078 513 Size: 552 939 95 19 6 954 3385 Fragments arranged by size: 3385 954 939 552 95 19 6 NdeI CA'TA_TG Cuts at: 387 2776 387 Size: 2389 3561 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5950 NotI GC'GGCC_GC Cuts at: 3397 3397 Size: 5950 NsiI A_TGCA'T Cuts at: 2353 3204 2353 Size: 851 5099 NspI r_CATG'y Cuts at: 1132 1229 2797 3308 4853 1132 Size: 97 1568 511 1545 2229 Fragments arranged by size: 2229 1568 1545 511 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5950 PciI A'CATG_T Cuts at: 4849 4849 Size: 5950 PflMI CCAn_nnn'nTGG Cuts at: 1108 2780 3079 1108 Size: 1672 299 3979 Fragments arranged by size: 3979 1672 299 PfoI T'CCnGG_A Cuts at: 2415 2415 Size: 5950 PmeI GTTT'AAAC Cuts at: 3254 3254 Size: 5950 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4019 4051 5532 5564 4019 Size: 32 1481 32 4405 Fragments arranged by size: 4405 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5950 PsiI TTA'TAA Cuts at: 3520 3520 Size: 5950 PstI C_TGCA'G Cuts at: 830 2305 3302 830 Size: 1475 997 3478 Fragments arranged by size: 3478 1475 997 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5950 PvuII CAG'CTG Cuts at: 2238 2238 Size: 5950 SalI G'TCGA_C Cuts at: 1936 3292 1936 Size: 1356 4594 SapI GCTCTTCn'nnn_ Cuts at: 2943 2943 Size: 5950 SbfI CC_TGCA'GG Cuts at: 3302 3302 Size: 5950 ScaI AGT'ACT Cuts at: 4198 5910 4198 Size: 1712 4238 SfcI C'TryA_G Cuts at: 826 1045 2301 2571 3298 4433 5114 5305 826 Size: 219 1256 270 727 1135 681 191 1471 Fragments arranged by size: 1471 1256 1135 727 681 270 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5950 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5950 SmaI CCC'GGG Cuts at: 1872 3277 1872 Size: 1405 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4054 4955 5217 820 Size: 197 926 2111 901 262 1553 Fragments arranged by size: 2111 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3741 493 Size: 3248 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5950 SphI G_CATG'C Cuts at: 1132 3308 1132 Size: 2176 3774 SspI AAT'ATT Cuts at: 5 52 2447 3874 5 Size: 47 2395 1427 2081 Fragments arranged by size: 2395 2081 1427 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5950 StyI C'CwwG_G Cuts at: 513 1065 2004 2099 2118 2124 2169 3078 3389 Size: 552 939 95 19 6 45 909 311 Cuts at: 3389 513 Size: 3074 Fragments arranged by size: 3074 939 909 552 311 95 45 19 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2093 3977 3994 4147 4332 4777 325 Size: 1255 513 1884 17 153 185 445 1498 Fragments arranged by size: 1884 1498 1255 513 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2698 2709 4196 Size: 274 80 33 51 1443 721 11 1487 Cuts at: 4196 5908 96 Size: 1712 138 Fragments arranged by size: 1712 1487 1443 721 274 138 80 51 33 11 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5910 5942 5910 Size: 32 5918 Tth111I GACn'n_nGTC Cuts at: 1316 2436 1316 Size: 1120 4830 XbaI T'CTAG_A Cuts at: 2764 3286 2764 Size: 522 5428 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2122 2131 2593 2122 Size: 9 462 5479 Fragments arranged by size: 5479 462 9 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5950 XmaI C'CCGG_G Cuts at: 1870 3275 1870 Size: 1405 4545 XmnI GAAnn'nnTTC Cuts at: 2087 2427 4079 2087 Size: 340 1652 3958 Fragments arranged by size: 3958 1652 340 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3758 276 Size: 53 83 186 936 2224 2468 Fragments arranged by size: 2468 2224 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI