Restriction map for
pFN21AB6620
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# NO CUT ENZYMES ( 124 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaLI AscI AseI AsnI
Asp700I AspEI AspI AviII AvrII
BalI BamHI BbeI BbrPI BbvCI
BclI BfrI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaWI BseAI
BsiEI BsiWI BsmBI BspDI BspEI
BspHI BsrBI BsrDI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstYI BstZ17I Bsu36I CelII ClaI
CpoI DraI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KasI KspI
MaeII MamI MfeI MflI MluI
MluNI MroI MscI MseI MunI
MvnI NaeI NarI NcoI NgoMIV
NheI NotI NruI NsiI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SfiI
SfoI SfuI SnaBI SpeI SphI
SspI StuI SwaI TfiI Tru9I
Tth111I XbaI XhoII XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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Acc65I 1030 GGTACC G^GTACC
AcsI 255 GAATTC [GA]^AATT[CT]
AcyI 791 GGCGTC G[GA]^CG[CT]C
AflIII 244 ACATGT A^C[GA][CT]GT
AlwI 775 GGATC GGATC(4/5)
ApoI 255 GAATTC [GA]^AATT[CT]
Asp718I 1030 GGTACC G^GTACC
BcgI 330 CGAGGAGATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 910 GTATCC GTATCC(6/5)
BfaI 1234 CTAG C^TAG
BsaHI 791 GGCGTC G[GA]^CG[CT]C
BsaI 1127 GAGACC GGTCTC(1/5)
BsgI 345 CTGCAC GTGCAG(16/14)
BsmI 1040 GCATTC GAATGC(1/-1)
Bsp1407I 415 TGTACA T^GTACA
BspLU11I 244 ACATGT A^CATGT
BsrFI 1051 ACCGGC [GA]^CCGG[CT]
BsrGI 415 TGTACA T^GTACA
BstAPI 167 GCATCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1209 CCAGGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 545 GCGC GCG^C
Cfr10I 1051 ACCGGC [GA]^CCGG[CT]
DdeI 204 CTCAG C^T[ACGT]AG
DpnII 776 GATC ^GATC
DraIII 564 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1112 GACCACCGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1026 CCACGG C^C[GA][CT]GG
EaeI 683 TGGCCG [CT]^GGCC[GA]
EcoNI 1265 CCTCTAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 255 GAATTC G^AATTC
HaeII 544 AGCGCC [GA]GCGC^[CT]
HhaI 545 GCGC GCG^C
Hin1I 791 GGCGTC G[GA]^CG[CT]C
HinP1I 545 GCGC G^CGC
HinfI 966 GAGTC G^A[ACGT]TC
KpnI 1030 GGTACC GGTAC^C
MaeI 1234 CTAG C^TAG
MboI 776 GATC ^GATC
NdeI 104 CATATG CA^TATG
NdeII 776 GATC ^GATC
NspI 244 ACATGT [GA]CATG^[CT]
PflMI 579 CCAAGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 966 GAGTC GAGTC(4/5)
PvuII 295 CAGCTG CAG^CTG
SacI 463 GAGCTC GAGCT^C
SapI 744 GAAGAGC GCTCTTC(1/4)
Sau3AI 776 GATC ^GATC
SbfI 125 CCTGCAGG CCTGCA^GG
ScaI 1101 AGTACT AGT^ACT
SexAI 565 ACCAGGT A^CC[AT]GGT
SgrAI 1050 CACCGGCG C[GA]^CCGG[CT]G
Sse8387I 125 CCTGCAGG CCTGCA^GG
SspBI 415 TGTACA T^GTACA
Van91I 579 CCAAGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AlwNI 602 CAGGCACTG CAG[ACGT][ACGT][ACGT]^CTG
874 CAGCCACTG
ApaI 404 GGGCCC GGGCC^C
1275 GGGCCC
BbsI 360 GAAGAC GAAGAC(2/6)
727 GTCTTC
BglI 185 GCCCGCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
443 GCCCGCCTGGC
BpuAI 360 GAAGAC GAAGAC(2/6)
727 GTCTTC
BseRI 534 CTCCTC GAGGAG(10/8)
331 GAGGAG
BspMI 124 ACCTGC ACCTGC(4/8)
625 ACCTGC
BstF5I 222 CATCC GGATG(2/0)
850 CATCC
FokI 222 CATCC GGATG(9/13)
850 CATCC
HphI 1049 TCACC GGTGA(8/7)
1295 TCACC
MslI 672 CATGCTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1249 CACTGTGGTG
PaeR7I 328 CTCGAG C^TCGAG
409 CTCGAG
PstI 126 CTGCAG CTGCA^G
762 CTGCAG
SmaI 1003 CCCGGG CCC^GGG
1189 CCCGGG
TaqI 329 TCGA T^CGA
410 TCGA
Tsp45I 311 GTGAC ^GT[GC]AC
1048 GTCAC
Tsp509I 256 AATT ^AATT
1289 AATT
TthHB8I 329 TCGA T^CGA
410 TCGA
XhoI 328 CTCGAG C^TCGAG
409 CTCGAG
XmaCI 1003 CCCGGG C^CCGGG
1189 CCCGGG
XmaI 1003 CCCGGG C^CCGGG
1189 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AciI AfaI AluI AspHI AvaI
AvaII BanI BanII BbvI BcnI
BmyI BpmI BsaJI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrI BssKI BstNI Cac8I Cfr13I
DraII EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI HaeIII HapII HgaI
HpaII ItaI Ksp632I MaeIII MboII
MnlI MspA1I MspI MvaI MwoI
NciI NlaIII NlaIV PpuMI RsaI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TseI TspRI XcmI