(Circular) (Six-base) MAPSORT of: pFN21AB6620.seq Check: 1515 from: 1 to: 6010 pFN21AB6620 6010 bp With 182 enzymes: SgfI * October 20, 2009 10:57 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3820 278 Size: 53 83 186 936 2284 2468 Fragments arranged by size: 2468 2284 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3353 1937 Size: 1416 4594 Acc65I G'GTAC_C Cuts at: 3035 3331 3035 Size: 296 5714 AclI AA'CG_TT Cuts at: 1563 4137 4510 1563 Size: 2574 373 3063 Fragments arranged by size: 3063 2574 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2481 4073 5457 5725 1632 Size: 849 1592 1384 268 1917 Fragments arranged by size: 1917 1592 1384 849 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5813 AflIII A'CryG_T Cuts at: 1203 2249 4909 5886 1203 Size: 1046 2660 977 1327 Fragments arranged by size: 2660 1327 1046 977 AgeI A'CCGG_T Cuts at: 4841 4841 Size: 6010 AhdI GACnn_n'nnGTC Cuts at: 4739 4739 Size: 6010 AleI CACnn'nnGTG Cuts at: 1166 3258 1166 Size: 2092 3918 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3276 1184 Size: 2092 3918 AlwNI CAG_nnn'CTG Cuts at: 2612 2884 3753 5325 2612 Size: 272 869 1572 3297 Fragments arranged by size: 3297 1572 869 272 ApaI G_GGCC'C Cuts at: 2413 3284 2413 Size: 871 5139 ApaLI G'TGCA_C Cuts at: 4067 5223 4067 Size: 1156 4854 ApoI r'AATT_y Cuts at: 1493 2260 3319 3545 3697 5806 1493 Size: 767 1059 226 152 2109 1697 Fragments arranged by size: 2109 1697 1059 767 226 152 AseI AT'TA_AT Cuts at: 160 1032 4564 160 Size: 872 3532 1606 Fragments arranged by size: 3532 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6010 AvaI C'yCGr_G Cuts at: 1870 1943 2333 2414 3008 3194 3335 1870 Size: 73 390 81 594 186 141 4545 Fragments arranged by size: 4545 594 390 186 141 81 73 BamHI G'GATC_C Cuts at: 3340 3707 3340 Size: 367 5643 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2101 2198 2956 3035 3234 Size: 325 217 627 314 97 758 79 199 Cuts at: 3234 3260 3331 4786 618 Size: 26 71 1455 1842 Fragments arranged by size: 1842 1455 758 627 325 314 217 199 97 79 71 26 BanII G_rGCy'C Cuts at: 1391 2076 2163 2413 2472 2928 3201 3284 3329 Size: 685 87 250 59 456 273 83 45 Cuts at: 3329 1391 Size: 4072 Fragments arranged by size: 4072 685 456 273 250 87 83 59 45 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2372 2725 928 Size: 971 473 353 4213 Fragments arranged by size: 4213 971 473 353 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2324 2358 4201 4235 1903 Size: 34 50 34 303 34 1843 34 3678 Fragments arranged by size: 3678 1843 303 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2926 3902 5112 2926 Size: 976 1210 3824 Fragments arranged by size: 3824 1210 976 BclI T'GATC_A Cuts at: 1548 1548 Size: 6010 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2196 2454 4621 Size: 107 122 71 631 605 524 258 2167 Cuts at: 4621 136 Size: 1525 Fragments arranged by size: 2167 1525 631 605 524 258 122 107 71 BglII A'GATC_T Cuts at: 6005 6005 Size: 6010 BlpI GC'TnA_GC Cuts at: 3427 3427 Size: 6010 Bme1580I G_kGCm'C Cuts at: 1790 2413 2526 3284 4071 5227 1790 Size: 623 113 758 787 1156 2573 Fragments arranged by size: 2573 1156 787 758 623 113 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6010 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2315 2365 2611 Size: 416 53 83 399 24 898 50 246 Cuts at: 2611 2786 2906 2931 2933 3119 3741 4699 442 Size: 175 120 25 2 186 622 958 1753 Fragments arranged by size: 1753 958 898 622 416 399 246 186 175 120 83 53 50 25 24 2 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6010 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2589 2919 3150 4670 1239 Size: 1350 330 231 1520 2579 Fragments arranged by size: 2579 1520 1350 330 231 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2774 4135 5000 5298 2774 Size: 1361 865 298 3486 Fragments arranged by size: 3486 1361 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3126 4673 882 Size: 617 1627 1547 2219 Fragments arranged by size: 2219 1627 1547 617 BsaAI yAC'GTr Cuts at: 493 1204 3801 493 Size: 711 2597 2702 Fragments arranged by size: 2702 2597 711 BsaBI GATnn'nnATC Cuts at: 6004 6004 Size: 6010 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2797 3817 4199 4834 Size: 53 83 186 936 1264 1020 382 635 Cuts at: 4834 275 Size: 1451 Fragments arranged by size: 1451 1264 1020 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4443 4841 5115 5262 5673 4443 Size: 398 274 147 411 4780 Fragments arranged by size: 4780 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2984 3014 1178 Size: 30 165 30 1581 30 4174 Fragments arranged by size: 4174 1581 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2351 2530 1186 Size: 1165 179 4666 Fragments arranged by size: 4666 1165 179 BseYI C'CCAG_C Cuts at: 2059 2163 2546 2835 3139 5213 5709 2059 Size: 104 383 289 304 2074 496 2360 Fragments arranged by size: 2360 2074 496 383 304 289 104 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2335 1163 Size: 573 599 4838 Fragments arranged by size: 4838 599 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3460 4221 5249 1540 Size: 275 186 1459 761 1028 2301 Fragments arranged by size: 2301 1459 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2277 2472 3255 4071 4156 5227 2277 Size: 195 783 816 85 1071 3060 Fragments arranged by size: 3060 1071 816 783 195 85 BsmI GAATG_Cn' Cuts at: 3045 3521 3614 3045 Size: 476 93 5441 Fragments arranged by size: 5441 476 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2076 2163 2277 2413 2472 2526 2928 Size: 399 286 87 114 136 59 54 402 Cuts at: 2928 3201 3255 3284 3329 4071 4156 5227 1391 Size: 273 54 29 45 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 402 399 286 273 136 114 87 85 59 54 54 45 29 BspEI T'CCGG_A Cuts at: 5673 5673 Size: 6010 BspHI T'CATG_A Cuts at: 3899 5629 3899 Size: 1730 4280 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2138 2639 3365 844 Size: 1294 501 726 3489 Fragments arranged by size: 3489 1294 726 501 BsrBI CCG'CTC Cuts at: 1394 1823 3897 1394 Size: 429 2074 3507 Fragments arranged by size: 3507 2074 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4505 4679 65 Size: 1183 3257 174 1396 Fragments arranged by size: 3257 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3056 4654 4841 1700 Size: 110 1246 1598 187 2869 Fragments arranged by size: 2869 1598 1246 187 110 BsrGI T'GTAC_A Cuts at: 96 2420 96 Size: 2324 3686 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6010 BssSI C'ACGA_G Cuts at: 4070 5082 4070 Size: 1012 4998 BstAPI GCAn_nnn'nTGC Cuts at: 794 2178 794 Size: 1384 4626 BstBI TT'CG_AA Cuts at: 4814 4814 Size: 6010 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6010 BstXI CCAn_nnnn'nTGG Cuts at: 3221 3221 Size: 6010 BstYI r'GATC_y Cuts at: 1972 3340 3707 4092 4109 5561 5647 5659 6005 Size: 1368 367 385 17 1452 86 12 346 Cuts at: 6005 1972 Size: 1977 Fragments arranged by size: 1977 1452 1368 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 3031 513 Size: 552 1966 3492 Fragments arranged by size: 3492 1966 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2070 487 Size: 683 56 844 4427 Fragments arranged by size: 4427 844 683 56 BtsI GCAGTG_nn' Cuts at: 2425 2500 2610 2651 2882 3526 4320 4340 2425 Size: 75 110 41 231 644 794 20 4095 Fragments arranged by size: 4095 794 644 231 110 75 41 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6010 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5975 DraI TTT'AAA Cuts at: 3314 3314 Size: 6010 DraIII CAC_nnn'GTG Cuts at: 2574 2574 Size: 6010 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3123 5017 809 Size: 507 1807 1894 1802 Fragments arranged by size: 1894 1807 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2688 3457 4346 Size: 54 1425 50 275 18 858 769 889 Cuts at: 4346 5975 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 858 769 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3457 1537 Size: 275 1645 4090 Fragments arranged by size: 4090 1645 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2072 2744 3168 3940 1353 Size: 719 672 424 772 3423 Fragments arranged by size: 3423 772 719 672 424 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4597 4971 5117 387 Size: 1064 138 3008 374 146 1280 Fragments arranged by size: 3008 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2470 2470 Size: 6010 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2481 2589 2919 3150 4073 4670 5457 Size: 393 849 108 330 231 923 597 787 Cuts at: 5457 5725 1239 Size: 268 1524 Fragments arranged by size: 1524 923 849 787 597 393 330 268 231 108 EcoNI CCTnn'n_nnAGG Cuts at: 3274 3274 Size: 6010 EcoO109I rG'GnC_Cy Cuts at: 2048 2410 2813 3125 3181 3280 2048 Size: 362 403 312 56 99 4778 Fragments arranged by size: 4778 403 362 312 99 56 EcoRI G'AATT_C Cuts at: 2260 3319 3697 2260 Size: 1059 378 4573 Fragments arranged by size: 4573 1059 378 FspI TGC'GCA Cuts at: 4516 4516 Size: 6010 HaeII r_GCGC'y Cuts at: 1678 2553 5157 1678 Size: 875 2604 2531 Fragments arranged by size: 2604 2531 875 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2772 2804 4651 4683 4725 Size: 32 135 32 1200 32 1847 32 42 Cuts at: 4725 4757 1373 Size: 32 2626 Fragments arranged by size: 2626 1847 1200 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3354 3600 669 Size: 988 281 1416 246 3079 Fragments arranged by size: 3079 1416 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6010 HpaI GTT'AAC Cuts at: 3600 3600 Size: 6010 KpnI G_GTAC'C Cuts at: 3039 3335 3039 Size: 296 5714 MfeI C'AATT_G Cuts at: 3609 3609 Size: 6010 MluI A'CGCG_T Cuts at: 5886 5886 Size: 6010 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2097 2646 2982 5124 5308 1305 Size: 792 549 336 2142 184 2007 Fragments arranged by size: 2142 2007 792 549 336 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4188 Fragments arranged by size: 4188 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2681 3258 3968 4327 4486 518 Size: 648 1515 577 710 359 159 2042 Fragments arranged by size: 2042 1515 710 648 577 359 159 MspA1I CmG'CkG Cuts at: 1606 2187 2302 2379 2556 3426 4103 5251 5496 Size: 581 115 77 177 870 677 1148 245 Cuts at: 5496 1606 Size: 2120 Fragments arranged by size: 2120 1148 870 677 581 245 177 115 77 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5458 NdeI CA'TA_TG Cuts at: 387 2110 387 Size: 1723 4287 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6010 NotI GC'GGCC_GC Cuts at: 3457 3457 Size: 6010 NspI r_CATG'y Cuts at: 1132 1229 2253 3368 4913 1132 Size: 97 1024 1115 1545 2229 Fragments arranged by size: 2229 1545 1115 1024 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6010 PciI A'CATG_T Cuts at: 2249 4909 2249 Size: 2660 3350 PflMI CCAn_nnn'nTGG Cuts at: 1108 2590 1108 Size: 1482 4528 PfoI T'CCnGG_A Cuts at: 2229 3127 2229 Size: 898 5112 PmeI GTTT'AAAC Cuts at: 3314 3314 Size: 6010 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4079 4111 5592 5624 4079 Size: 32 1481 32 4465 Fragments arranged by size: 4465 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2813 3125 3181 2813 Size: 312 56 5642 Fragments arranged by size: 5642 312 56 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6010 PsiI TTA'TAA Cuts at: 3580 3580 Size: 6010 PspOMI G'GGCC_C Cuts at: 2409 3280 2409 Size: 871 5139 PspXI vC'TCGA_Gb Cuts at: 2333 2414 2333 Size: 81 5929 PstI C_TGCA'G Cuts at: 830 2135 2771 3362 830 Size: 1305 636 591 3478 Fragments arranged by size: 3478 1305 636 591 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6010 PvuII CAG'CTG Cuts at: 2302 2302 Size: 6010 SacI G_AGCT'C Cuts at: 2472 2472 Size: 6010 SalI G'TCGA_C Cuts at: 1936 3352 1936 Size: 1416 4594 SanDI GG'GwC_CC Cuts at: 2813 3181 2813 Size: 368 5642 SapI GCTCTTCn'nnn_ Cuts at: 2744 2744 Size: 6010 SbfI CC_TGCA'GG Cuts at: 2135 3362 2135 Size: 1227 4783 ScaI AGT'ACT Cuts at: 3108 4258 5970 3108 Size: 1150 1712 3148 Fragments arranged by size: 3148 1712 1150 SexAI A'CCwGG_T Cuts at: 2570 2570 Size: 6010 SfcI C'TryA_G Cuts at: 826 1045 2131 2767 2823 3358 4493 5174 5365 Size: 219 1086 636 56 535 1135 681 191 Cuts at: 5365 826 Size: 1471 Fragments arranged by size: 1471 1135 1086 681 636 535 219 191 56 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6010 SgrAI Cr'CCGG_yG Cuts at: 1700 3056 1700 Size: 1356 4654 SmaI CCC'GGG Cuts at: 1872 3010 3196 3337 1872 Size: 1138 186 141 4545 Fragments arranged by size: 4545 1138 186 141 SmlI C'TyrA_G Cuts at: 820 1017 1943 2333 2414 2789 4114 5015 5277 Size: 197 926 390 81 375 1325 901 262 Cuts at: 5277 820 Size: 1553 Fragments arranged by size: 1553 1325 926 901 390 375 262 197 81 SnaBI TAC'GTA Cuts at: 493 3801 493 Size: 3308 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6010 SphI G_CATG'C Cuts at: 1132 3368 1132 Size: 2236 3774 SspI AAT'ATT Cuts at: 5 52 3934 5 Size: 47 3882 2081 Fragments arranged by size: 3882 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6010 StyI C'CwwG_G Cuts at: 513 1065 2015 2584 3284 3449 513 Size: 552 950 569 700 165 3074 Fragments arranged by size: 3074 950 700 569 552 165 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3317 4037 4054 4207 4392 4837 325 Size: 1255 1737 720 17 153 185 445 1498 Fragments arranged by size: 1737 1498 1255 720 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2420 3106 4256 Size: 274 80 33 51 1443 443 686 1150 Cuts at: 4256 5968 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1150 686 443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5970 6002 5970 Size: 32 5978 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6010 XbaI T'CTAG_A Cuts at: 3346 3346 Size: 6010 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2683 3038 3218 2683 Size: 355 180 5475 Fragments arranged by size: 5475 355 180 XhoI C'TCGA_G Cuts at: 1943 2333 2414 1943 Size: 390 81 5539 Fragments arranged by size: 5539 390 81 XmaI C'CCGG_G Cuts at: 1870 3008 3194 3335 1870 Size: 1138 186 141 4545 Fragments arranged by size: 4545 1138 186 141 XmnI GAAnn'nnTTC Cuts at: 4139 4139 Size: 6010 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3818 276 Size: 53 83 186 936 2284 2468 Fragments arranged by size: 2468 2284 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PsrI RsrII SacII SfiI SfoI SrfI SwaI