Restriction map for
pFN21AB6620
(Linear) (Six-base) MAPSORT of: KIBB6620.seq Check: 7662 from: 1 to: 1305
KIBB6620.orf
With 182 enzymes: SgfI *
October 7, 2009 14:21 ..
Acc65I G'GTAC_C
Cuts at: 0 1030 1305
Size: 1030 275
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 476 1305
Size: 476 829
AflIII A'CryG_T
Cuts at: 0 244 1305
Size: 244 1061
AleI CACnn'nnGTG
Cuts at: 0 1253 1305
Size: 1253 52
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1271 1305
Size: 1271 34
AlwNI CAG_nnn'CTG
Cuts at: 0 607 879 1305
Size: 607 272 426
Fragments arranged by size:
607 426 272
ApaI G_GGCC'C
Cuts at: 0 408 1279 1305
Size: 408 871 26
Fragments arranged by size:
871 408 26
ApoI r'AATT_y
Cuts at: 0 255 1305
Size: 255 1050
AvaI C'yCGr_G
Cuts at: 0 328 409 1003 1189 1305
Size: 328 81 594 186 116
Fragments arranged by size:
594 328 186 116 81
BanI G'GyrC_C
Cuts at: 0 96 193 951 1030 1229 1255 1305
Size: 96 97 758 79 199 26 50
Fragments arranged by size:
758 199 97 96 79 50 26
BanII G_rGCy'C
Cuts at: 0 71 158 408 467 923 1196 1279 1305
Size: 71 87 250 59 456 273 83 26
Fragments arranged by size:
456 273 250 87 83 71 59 26
BbsI GAAGACnn'nnnn_
Cuts at: 0 367 720 1305
Size: 367 353 585
Fragments arranged by size:
585 367 353
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 319 353 1305
Size: 319 34 952
Fragments arranged by size:
952 319 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 921 1305
Size: 921 384
BglI GCCn_nnn'nGGC
Cuts at: 0 191 449 1305
Size: 191 258 856
Fragments arranged by size:
856 258 191
Bme1580I G_kGCm'C
Cuts at: 0 408 521 1279 1305
Size: 408 113 758 26
Fragments arranged by size:
758 408 113 26
BmrI ACTGGGnnnn_n'
Cuts at: 0 310 360 606 781 901 926 928 1114
Size: 310 50 246 175 120 25 2 186
Cuts at: 1114 1305
Size: 191
Fragments arranged by size:
310 246 191 186 175 120 50 25
2
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 584 914 1145 1305
Size: 584 330 231 160
Fragments arranged by size:
584 330 231 160
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 769 1305
Size: 769 536
BsaI GGTCTCn'nnnn_
Cuts at: 0 1121 1305
Size: 1121 184
BsaHI Gr'CG_yC
Cuts at: 0 792 1305
Size: 792 513
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 979 1009 1305
Size: 979 30 296
Fragments arranged by size:
979 296 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 346 525 1305
Size: 346 179 780
Fragments arranged by size:
780 346 179
BseYI C'CCAG_C
Cuts at: 0 54 158 541 830 1134 1305
Size: 54 104 383 289 304 171
Fragments arranged by size:
383 304 289 171 104 54
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 330 1305
Size: 330 975
BsiHKAI G_wGCw'C
Cuts at: 0 272 467 1250 1305
Size: 272 195 783 55
Fragments arranged by size:
783 272 195 55
BsmI GAATG_Cn'
Cuts at: 0 1040 1305
Size: 1040 265
Bsp1286I G_dGCh'C
Cuts at: 0 71 158 272 408 467 521 923 1196
Size: 71 87 114 136 59 54 402 273
Cuts at: 1196 1250 1279 1305
Size: 54 29 26
Fragments arranged by size:
402 273 136 114 87 71 59 54
54 29 26
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 133 634 1305
Size: 133 501 671
Fragments arranged by size:
671 501 133
BsrFI r'CCGG_y
Cuts at: 0 1051 1305
Size: 1051 254
BsrGI T'GTAC_A
Cuts at: 0 415 1305
Size: 415 890
BstAPI GCAn_nnn'nTGC
Cuts at: 0 173 1305
Size: 173 1132
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1216 1305
Size: 1216 89
BtgI C'CryG_G
Cuts at: 0 1026 1305
Size: 1026 279
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 65 1305
Size: 65 1240
BtsI GCAGTG_nn'
Cuts at: 0 420 495 605 646 877 1305
Size: 420 75 110 41 231 428
Fragments arranged by size:
428 420 231 110 75 41
DraIII CAC_nnn'GTG
Cuts at: 0 569 1305
Size: 569 736
DrdI GACnn_nn'nnGTC
Cuts at: 0 1118 1305
Size: 1118 187
EaeI y'GGCC_r
Cuts at: 0 683 1305
Size: 683 622
EarI CTCTTCn'nnn_
Cuts at: 0 67 739 1163 1305
Size: 67 672 424 142
Fragments arranged by size:
672 424 142 67
EcoICRI GAG'CTC
Cuts at: 0 465 1305
Size: 465 840
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 476 584 914 1145 1305
Size: 476 108 330 231 160
Fragments arranged by size:
476 330 231 160 108
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1269 1305
Size: 1269 36
EcoO109I rG'GnC_Cy
Cuts at: 0 43 405 808 1120 1176 1275 1305
Size: 43 362 403 312 56 99 30
Fragments arranged by size:
403 362 312 99 56 43 30
EcoRI G'AATT_C
Cuts at: 0 255 1305
Size: 255 1050
HaeII r_GCGC'y
Cuts at: 0 548 1305
Size: 548 757
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 767 799 1305
Size: 767 32 506
Fragments arranged by size:
767 506 32
KpnI G_GTAC'C
Cuts at: 0 1034 1305
Size: 1034 271
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 92 641 977 1305
Size: 92 549 336 328
Fragments arranged by size:
549 336 328 92
MslI CAynn'nnrTG
Cuts at: 0 676 1253 1305
Size: 676 577 52
Fragments arranged by size:
676 577 52
MspA1I CmG'CkG
Cuts at: 0 182 297 374 551 1305
Size: 182 115 77 177 754
Fragments arranged by size:
754 182 177 115 77
NdeI CA'TA_TG
Cuts at: 0 105 1305
Size: 105 1200
NspI r_CATG'y
Cuts at: 0 248 1305
Size: 248 1057
PciI A'CATG_T
Cuts at: 0 244 1305
Size: 244 1061
PflMI CCAn_nnn'nTGG
Cuts at: 0 585 1305
Size: 585 720
PfoI T'CCnGG_A
Cuts at: 0 224 1122 1305
Size: 224 898 183
Fragments arranged by size:
898 224 183
PpuMI rG'GwC_Cy
Cuts at: 0 808 1120 1176 1305
Size: 808 312 56 129
Fragments arranged by size:
808 312 129 56
PspOMI G'GGCC_C
Cuts at: 0 404 1275 1305
Size: 404 871 30
Fragments arranged by size:
871 404 30
PspXI vC'TCGA_Gb
Cuts at: 0 328 409 1305
Size: 328 81 896
Fragments arranged by size:
896 328 81
PstI C_TGCA'G
Cuts at: 0 130 766 1305
Size: 130 636 539
Fragments arranged by size:
636 539 130
PvuII CAG'CTG
Cuts at: 0 297 1305
Size: 297 1008
SacI G_AGCT'C
Cuts at: 0 467 1305
Size: 467 838
SanDI GG'GwC_CC
Cuts at: 0 808 1176 1305
Size: 808 368 129
Fragments arranged by size:
808 368 129
SapI GCTCTTCn'nnn_
Cuts at: 0 739 1305
Size: 739 566
SbfI CC_TGCA'GG
Cuts at: 0 130 1305
Size: 130 1175
ScaI AGT'ACT
Cuts at: 0 1103 1305
Size: 1103 202
SexAI A'CCwGG_T
Cuts at: 0 565 1305
Size: 565 740
SfcI C'TryA_G
Cuts at: 0 126 762 818 1305
Size: 126 636 56 487
Fragments arranged by size:
636 487 126 56
SgrAI Cr'CCGG_yG
Cuts at: 0 1051 1305
Size: 1051 254
SmaI CCC'GGG
Cuts at: 0 1005 1191 1305
Size: 1005 186 114
Fragments arranged by size:
1005 186 114
SmlI C'TyrA_G
Cuts at: 0 328 409 784 1305
Size: 328 81 375 521
Fragments arranged by size:
521 375 328 81
StyI C'CwwG_G
Cuts at: 0 10 579 1279 1305
Size: 10 569 700 26
Fragments arranged by size:
700 569 26 10
TatI w'GTAC_w
Cuts at: 0 415 1101 1305
Size: 415 686 204
Fragments arranged by size:
686 415 204
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 678 1033 1213 1305
Size: 678 355 180 92
Fragments arranged by size:
678 355 180 92
XhoI C'TCGA_G
Cuts at: 0 328 409 1305
Size: 328 81 896
Fragments arranged by size:
896 328 81
XmaI C'CCGG_G
Cuts at: 0 1003 1189 1305
Size: 1003 186 116
Fragments arranged by size:
1003 186 116
Enzymes that do cut:
Acc65I AcuI AflIII AleI AlfI AlwNI ApaI ApoI
AvaI BanI BanII BbsI BcgI BciVI BglI Bme1580I
BmrI BpmI BpuEI BsaI BsaHI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI Bsp1286I BspMI BsrFI BsrGI BstAPI
BstXI BtgI BtgZI BtsI DraIII DrdI EaeI EarI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI HaeII Hin4I KpnI
MmeI MslI MspA1I NdeI NspI PciI PflMI PfoI
PpuMI PspOMI PspXI PstI PvuII SacI SanDI SapI
SbfI ScaI SexAI SfcI SgrAI SmaI SmlI StyI
TatI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AgeI AhdI
AloI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BbvCI BclI BfrBI BglII BlpI BmgBI BmtI
BplI Bpu10I BsaAI BsaBI BsaWI BsiEI BsiWI BsmBI
BspEI BspHI BsrBI BsrDI BssHII BssSI BstBI BstEII
BstYI BstZ17I Bsu36I ClaI CspCI DraI EagI EciI
EcoRV FalI FseI FspI FspAI HincII HindIII HpaI
KasI MfeI MluI MscI NaeI NarI NcoI NgoMIV
NheI NotI NruI NsiI PacI PasI PmeI PmlI
PpiI PshAI PsiI PsrI PvuI RsrII SacII SalI
SfiI SfoI SgfI SnaBI SpeI SphI SrfI SspI
StuI SwaI TaqII TstI Tth111I XbaI XmnI ZraI