Restriction map for
pFN21AB6619
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# NO CUT ENZYMES ( 107 enzymes) #
##################################
Acc65I AccIII AclI AflII AflIII
AhdI Alw44I AlwI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspI AvrII BamHI BbrPI
BbvCI BcgI BciVI BclI BfaI
BfrI BglII BlnI BsaAI BseAI
BsiWI BsmI BspDI BspEI BspHI
BspLU11I BsrBI BssHII BstAPI BstBI
BstXI BstYI ClaI CpoI DraI
DrdI Eam1105I Eco47III EcoNI EcoRV
EcoT22I FbaI HgaI HincII HindII
HpaI KpnI KspI MaeI MfeI
MflI MluI MroI MseI MunI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PflFI PflMI
PmaCI PmeI PmlI PshBI Psp1406I
PstI PvuI RcaI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI SwaI Tru9I
Tth111I Van91I XbaI XcmI XhoII
XmaCI XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AatII 88 GACGTC GACGT^C
AccI 963 GTATAC GT^[AC][GT]AC
AccII 771 CGCG CG^CG
AlwNI 282 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1188 CCTCAGG CC^T[ACGT]AGG
Asp700I 810 GAACCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 917 TGCGCA TGC^GCA
BbeI 349 GGCGCC GGCGC^C
BbvI 392 GCTGC GCAGC(8/12)
BglI 97 GCCTATCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 504 GATTCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 1083 CGGCCG CG[GA][CT]^CG
BsmBI 1210 CGTCTC CGTCTC(1/5)
Bsp1407I 1073 TGTACA T^GTACA
BspMI 1047 GCAGGT ACCTGC(4/8)
BsrGI 1073 TGTACA T^GTACA
Bst1107I 963 GTATAC GTA^TAC
BstEII 702 GGTGACC G^GT[ACGT]ACC
BstPI 702 GGTGACC G^GT[ACGT]ACC
BstUI 771 CGCG CG^CG
BstZ17I 963 GTATAC GTA^TAC
Bsu36I 1188 CCTCAGG CC^T[ACGT]AGG
DraIII 48 CACCTCGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 1083 CGGCCG C^GGCCG
EarI 748 CTCTTC CTCTTC(1/4)
EclXI 1083 CGGCCG C^GGCCG
Eco52I 1083 CGGCCG C^GGCCG
Eco81I 1188 CCTCAGG CC^T[ACGT]AGG
EcoO65I 702 GGTGACC G^GT[ACGT]ACC
EcoRI 445 GAATTC G^AATTC
FseI 221 GGCCGGCC GGCCGG^CC
FspI 917 TGCGCA TGC^GCA
HindIII 157 AAGCTT A^AGCTT
KasI 349 GGCGCC G^GCGCC
Ksp632I 748 CTCTTC CTCTTC(1/4)
MamI 504 GATTCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 771 CGCG CG^CG
NaeI 222 GCCGGC GCC^GGC
NarI 349 GGCGCC GG^CGCC
NcoI 902 CCATGG C^CATGG
NgoMIV 222 GCCGGC G^CCGGC
PaeR7I 667 CTCGAG C^TCGAG
PleI 1090 GAGTC GAGTC(4/5)
PshAI 705 GACCAACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 390 CAGCTG CAG^CTG
SapI 747 GCTCTTC GCTCTTC(1/4)
SfcI 30 CTACAG C^T[GA][CT]AG
SfoI 349 GGCGCC GGC^GCC
SspBI 1073 TGTACA T^GTACA
TseI 392 GCTGC G^C[AT]GC
XhoI 667 CTCGAG C^TCGAG
XmnI 810 GAACCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AcyI 88 GACGTC G[GA]^CG[CT]C
349 GGCGCC
AgeI 455 ACCGGT A^CCGGT
967 ACCGGT
AspHI 385 GAGCTC G[AT]GC[AT]^C
745 GAGCTC
AvaI 667 CTCGAG C^[CT]CG[GA]G
833 CCCGAG
BalI 104 TGGCCA TGG^CCA
114 TGGCCA
BanII 385 GAGCTC G[GA]GC[CT]^C
745 GAGCTC
BbsI 124 GAAGAC GAAGAC(2/6)
624 GAAGAC
BlpI 387 GCTCAGC GC^T[ACGT]AGC
952 GCTGAGC
Bpu1102I 387 GCTCAGC GC^T[ACGT]AGC
952 GCTGAGC
BpuAI 124 GAAGAC GAAGAC(2/6)
624 GAAGAC
BsaHI 88 GACGTC G[GA]^CG[CT]C
349 GGCGCC
BsaI 847 GAGACC GGTCTC(1/5)
613 GGTCTC
BseRI 208 GAGGAG GAGGAG(10/8)
1114 GAGGAG
BsgI 1045 GTGCAG GTGCAG(16/14)
1135 GTGCAG
BsiHKAI 385 GAGCTC G[AT]GC[AT]^C
745 GAGCTC
BsoBI 667 CTCGAG C^[CT]CG[GA]G
833 CCCGAG
BsrI 402 ACTGG ACTGG(1/-1)
231 CCAGT
BssSI 51 CTCGTG CACGAG(-5/-1)
61 CTCGTG
CelII 387 GCTCAGC GC^T[ACGT]AGC
952 GCTGAGC
DpnII 370 GATC ^GATC
672 GATC
EcoT14I 740 CCTTGG C^C[AT][AT]GG
902 CCATGG
HaeII 349 GGCGCC [GA]GCGC^[CT]
603 AGCGCC
Hin1I 88 GACGTC G[GA]^CG[CT]C
349 GGCGCC
MboI 370 GATC ^GATC
672 GATC
MluNI 104 TGGCCA TGG^CCA
114 TGGCCA
MscI 104 TGGCCA TGG^CCA
114 TGGCCA
MspA1I 319 CAGCGG C[AC]G^C[GT]G
390 CAGCTG
NdeII 370 GATC ^GATC
672 GATC
PinAI 455 ACCGGT A^CCGGT
967 ACCGGT
PpuMI 632 AGGACCC [GA]G^G[AT]CC[CT]
689 GGGTCCC
SacI 385 GAGCTC GAGCT^C
745 GAGCTC
Sau3AI 370 GATC ^GATC
672 GATC
SmlI 667 CTCGAG C^T[CT][GA]AG
1060 CTCAAG
StuI 893 AGGCCT AGG^CCT
933 AGGCCT
StyI 740 CCTTGG C^C[AT][AT]GG
902 CCATGG
TfiI 504 GATTC G^A[AT]TC
578 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AvaII BanI BcnI BmyI BpmI
BsaJI BsaWI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrDI BsrFI BssKI
BstF5I BstNI Cac8I CfoI Cfr10I
Cfr13I DdeI DraII DsaI EaeI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeII MaeIII
MboII MnlI MslI MspI MvaI
MwoI NciI NlaIII NlaIV RsaI
Sau96I ScrFI SfaNI TaqI Tsp45I
Tsp509I TspRI TthHB8I