(Circular) (Six-base) MAPSORT of: pFN21AB6619.seq Check: 4233 from: 1 to: 5941 pFN21AB6619 5941 bp With 182 enzymes: SgfI * October 20, 2009 10:57 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3043 3043 Size: 5941 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2097 3751 278 Size: 53 83 186 936 561 1654 2468 Fragments arranged by size: 2468 1654 936 561 186 83 53 AccI GT'mk_AC Cuts at: 1937 2969 3284 1937 Size: 1032 315 4594 Fragments arranged by size: 4594 1032 315 Acc65I G'GTAC_C Cuts at: 3262 3262 Size: 5941 AclI AA'CG_TT Cuts at: 1563 4068 4441 1563 Size: 2505 373 3063 Fragments arranged by size: 3063 2505 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2096 2180 3221 4004 5388 5656 1632 Size: 464 84 1041 783 1384 268 1917 Fragments arranged by size: 1917 1384 1041 783 464 268 84 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5744 AflIII A'CryG_T Cuts at: 1203 4840 5817 1203 Size: 3637 977 1327 Fragments arranged by size: 3637 1327 977 AgeI A'CCGG_T Cuts at: 2460 2972 4772 2460 Size: 512 1800 3629 Fragments arranged by size: 3629 1800 512 AhdI GACnn_n'nnGTC Cuts at: 4670 4670 Size: 5941 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5941 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3155 1184 Size: 1971 3970 AlwNI CAG_nnn'CTG Cuts at: 2292 3684 5256 2292 Size: 1392 1572 2977 Fragments arranged by size: 2977 1572 1392 ApaLI G'TGCA_C Cuts at: 3998 5154 3998 Size: 1156 4785 ApoI r'AATT_y Cuts at: 1493 2450 2951 2999 3250 3476 3628 5737 1493 Size: 957 501 48 251 226 152 2109 1697 Fragments arranged by size: 2109 1697 957 501 251 226 152 48 AseI AT'TA_AT Cuts at: 160 1032 4495 160 Size: 872 3463 1606 Fragments arranged by size: 3463 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5941 AvaI C'yCGr_G Cuts at: 1870 1943 2672 2838 3266 1870 Size: 73 729 166 428 4545 Fragments arranged by size: 4545 729 428 166 73 BamHI G'GATC_C Cuts at: 3271 3638 3271 Size: 367 5574 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2273 2354 2726 2864 3262 Size: 325 217 627 486 81 372 138 398 Cuts at: 3262 4717 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 486 398 372 325 217 138 81 BanII G_rGCy'C Cuts at: 1391 2394 2754 3260 1391 Size: 1003 360 506 4072 Fragments arranged by size: 4072 1003 506 360 BbeI G_GCGC'C Cuts at: 2358 2358 Size: 5941 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2136 2636 928 Size: 971 237 500 4233 Fragments arranged by size: 4233 971 500 237 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4132 4166 1903 Size: 34 2195 34 3678 Fragments arranged by size: 3678 2195 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3833 5043 3833 Size: 1210 4731 BclI T'GATC_A Cuts at: 1548 1548 Size: 5941 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2108 4552 136 Size: 107 122 71 631 605 436 2444 1525 Fragments arranged by size: 2444 1525 631 605 436 122 107 71 BglII A'GATC_T Cuts at: 5936 5936 Size: 5941 BlpI GC'TnA_GC Cuts at: 2393 2958 3358 2393 Size: 565 400 4976 Fragments arranged by size: 4976 565 400 Bme1580I G_kGCm'C Cuts at: 1790 2276 4002 5158 1790 Size: 486 1726 1156 2573 Fragments arranged by size: 2573 1726 1156 486 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5941 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3672 4630 442 Size: 416 53 83 399 24 2255 958 1753 Fragments arranged by size: 2255 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5941 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2458 2598 2609 3203 4601 1239 Size: 1219 140 11 594 1398 2579 Fragments arranged by size: 2579 1398 1219 594 140 11 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3050 4066 4931 5229 3050 Size: 1016 865 298 3762 Fragments arranged by size: 3762 1016 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2624 2846 4604 882 Size: 617 1125 222 1758 2219 Fragments arranged by size: 2219 1758 1125 617 222 BsaAI yAC'GTr Cuts at: 493 1204 3732 493 Size: 711 2528 2702 Fragments arranged by size: 2702 2528 711 BsaBI GATnn'nnATC Cuts at: 2513 5935 2513 Size: 3422 2519 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2094 2355 3748 4130 Size: 53 83 186 936 561 261 1393 382 Cuts at: 4130 4765 275 Size: 635 1451 Fragments arranged by size: 1451 1393 936 635 561 382 261 186 83 53 BsaWI w'CCGG_w Cuts at: 2382 2460 2972 4374 4772 5046 5193 5604 2382 Size: 78 512 1402 398 274 147 411 2719 Fragments arranged by size: 2719 1402 512 411 398 274 147 78 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2726 2756 1178 Size: 30 165 30 1323 30 4363 Fragments arranged by size: 4363 1323 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2228 3134 1186 Size: 1042 906 3993 Fragments arranged by size: 3993 1042 906 BseYI C'CCAG_C Cuts at: 2322 2641 2857 5144 5640 2322 Size: 319 216 2287 496 2623 Fragments arranged by size: 2623 2287 496 319 216 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3071 3161 1163 Size: 573 1335 90 3943 Fragments arranged by size: 3943 1335 573 90 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3091 3391 4152 5180 1540 Size: 275 186 1090 300 761 1028 2301 Fragments arranged by size: 2301 1090 1028 761 300 275 186 BsiHKAI G_wGCw'C Cuts at: 2394 2754 4002 4087 5158 2394 Size: 360 1248 85 1071 3177 Fragments arranged by size: 3177 1248 1071 360 85 BsmI GAATG_Cn' Cuts at: 3452 3545 3452 Size: 93 5848 BsmBI CGTCTCn'nnnn_ Cuts at: 3221 3221 Size: 5941 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2276 2394 2754 3260 4002 4087 5158 Size: 399 486 118 360 506 742 85 1071 Cuts at: 5158 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 506 486 399 360 118 85 BspEI T'CCGG_A Cuts at: 5604 5604 Size: 5941 BspHI T'CATG_A Cuts at: 3830 5560 3830 Size: 1730 4211 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3043 3296 844 Size: 2199 253 3489 Fragments arranged by size: 3489 2199 253 BsrBI CCG'CTC Cuts at: 1394 1823 3828 1394 Size: 429 2005 3507 Fragments arranged by size: 3507 2005 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2097 2427 2482 4436 4610 65 Size: 1183 849 330 55 1954 174 1396 Fragments arranged by size: 1954 1396 1183 849 330 174 55 BsrFI r'CCGG_y Cuts at: 1700 1810 2227 2460 2972 4585 4772 1700 Size: 110 417 233 512 1613 187 2869 Fragments arranged by size: 2869 1613 512 417 233 187 110 BsrGI T'GTAC_A Cuts at: 96 3078 96 Size: 2982 2959 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5941 BssSI C'ACGA_G Cuts at: 2056 2066 4001 5013 2056 Size: 10 1935 1012 2984 Fragments arranged by size: 2984 1935 1012 10 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5941 BstBI TT'CG_AA Cuts at: 4745 4745 Size: 5941 BstEII G'GTnAC_C Cuts at: 1184 2707 1184 Size: 1523 4418 BstYI r'GATC_y Cuts at: 1972 3271 3638 4023 4040 5492 5578 5590 5936 Size: 1299 367 385 17 1452 86 12 346 Cuts at: 5936 1972 Size: 1977 Fragments arranged by size: 1977 1452 1299 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2970 2970 Size: 5941 Bsu36I CC'TnA_GG Cuts at: 3194 3194 Size: 5941 BtgI C'CryG_G Cuts at: 513 1065 2022 2350 2537 2907 513 Size: 552 957 328 187 370 3547 Fragments arranged by size: 3547 957 552 370 328 187 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2281 3001 487 Size: 683 56 1055 720 3427 Fragments arranged by size: 3427 1055 720 683 56 BtsI GCAGTG_nn' Cuts at: 3010 3231 3457 4251 4271 3010 Size: 221 226 794 20 4680 Fragments arranged by size: 4680 794 226 221 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5941 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5906 DraI TTT'AAA Cuts at: 3245 3245 Size: 5941 DraIII CAC_nnn'GTG Cuts at: 2058 2058 Size: 5941 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4948 809 Size: 507 3632 1802 Fragments arranged by size: 3632 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2109 2119 2225 Size: 54 1425 50 275 18 279 10 106 Cuts at: 2225 2229 3088 3388 4277 5906 8 Size: 4 859 300 889 1629 43 Fragments arranged by size: 1629 1425 889 859 300 279 275 106 54 50 43 18 10 4 EagI C'GGCC_G Cuts at: 1537 1812 3088 3388 1537 Size: 275 1276 300 4090 Fragments arranged by size: 4090 1276 300 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2759 3871 1353 Size: 1406 1112 3423 Fragments arranged by size: 3423 1406 1112 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2996 4528 4902 5048 387 Size: 1064 138 1407 1532 374 146 1280 Fragments arranged by size: 1532 1407 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2392 2752 2392 Size: 360 5581 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2096 2180 2458 2598 2609 3203 3221 Size: 393 464 84 278 140 11 594 18 Cuts at: 3221 4004 4601 5388 5656 1239 Size: 783 597 787 268 1524 Fragments arranged by size: 1524 787 783 597 594 464 393 278 268 140 84 18 11 EcoO109I rG'GnC_Cy Cuts at: 2289 2638 2695 2289 Size: 349 57 5535 Fragments arranged by size: 5535 349 57 EcoRI G'AATT_C Cuts at: 2450 3250 3628 2450 Size: 800 378 4763 Fragments arranged by size: 4763 800 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2149 2149 Size: 5941 FseI GG_CCGG'CC Cuts at: 2231 2231 Size: 5941 FspI TGC'GCA Cuts at: 2924 4447 2924 Size: 1523 4418 HaeII r_GCGC'y Cuts at: 1678 2358 2612 5088 1678 Size: 680 254 2476 2531 Fragments arranged by size: 2531 2476 680 254 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4582 4614 4656 4688 1373 Size: 32 135 32 3010 32 42 32 2626 Fragments arranged by size: 3010 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3285 3531 669 Size: 988 281 1347 246 3079 Fragments arranged by size: 3079 1347 988 281 246 HindIII A'AGCT_T Cuts at: 748 2162 748 Size: 1414 4527 HpaI GTT'AAC Cuts at: 3531 3531 Size: 5941 KasI G'GCGC_C Cuts at: 2354 2354 Size: 5941 KpnI G_GTAC'C Cuts at: 3266 3266 Size: 5941 MfeI C'AATT_G Cuts at: 3540 3540 Size: 5941 MluI A'CGCG_T Cuts at: 5817 5817 Size: 5941 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5055 5239 1305 Size: 3750 184 2007 Fragments arranged by size: 3750 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2111 2121 10 Size: 54 1425 343 279 10 3830 Fragments arranged by size: 3830 1425 343 279 54 10 MslI CAynn'nnrTG Cuts at: 518 1166 2066 2828 3137 3899 4258 4417 518 Size: 648 900 762 309 762 359 159 2042 Fragments arranged by size: 2042 900 762 762 648 359 309 159 MspA1I CmG'CkG Cuts at: 1606 2326 2397 3357 4034 5182 5427 1606 Size: 720 71 960 677 1148 245 2120 Fragments arranged by size: 2120 1148 960 720 677 245 71 NaeI GCC'GGC Cuts at: 2229 2229 Size: 5941 NarI GG'CG_CC Cuts at: 2355 2355 Size: 5941 NcoI C'CATG_G Cuts at: 513 1065 2907 513 Size: 552 1842 3547 Fragments arranged by size: 3547 1842 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5941 NgoMIV G'CCGG_C Cuts at: 2227 2227 Size: 5941 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5941 NotI GC'GGCC_GC Cuts at: 3388 3388 Size: 5941 NspI r_CATG'y Cuts at: 1132 1229 3299 4844 1132 Size: 97 2070 1545 2229 Fragments arranged by size: 2229 2070 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5941 PciI A'CATG_T Cuts at: 4840 4840 Size: 5941 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5941 PfoI T'CCnGG_A Cuts at: 2586 2586 Size: 5941 PmeI GTTT'AAAC Cuts at: 3245 3245 Size: 5941 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2666 2698 4010 4042 5523 5555 2666 Size: 32 1312 32 1481 32 3052 Fragments arranged by size: 3052 1481 1312 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2638 2695 2638 Size: 57 5884 PshAI GACnn'nnGTC Cuts at: 1613 2714 1613 Size: 1101 4840 PsiI TTA'TAA Cuts at: 3511 3511 Size: 5941 PstI C_TGCA'G Cuts at: 830 3293 830 Size: 2463 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5941 PvuII CAG'CTG Cuts at: 2397 2397 Size: 5941 SacI G_AGCT'C Cuts at: 2394 2754 2394 Size: 360 5581 SalI G'TCGA_C Cuts at: 1936 3283 1936 Size: 1347 4594 SanDI GG'GwC_CC Cuts at: 2695 2695 Size: 5941 SapI GCTCTTCn'nnn_ Cuts at: 2759 2759 Size: 5941 SbfI CC_TGCA'GG Cuts at: 3293 3293 Size: 5941 ScaI AGT'ACT Cuts at: 4189 5901 4189 Size: 1712 4229 SfcI C'TryA_G Cuts at: 826 1045 2035 3289 4424 5105 5296 826 Size: 219 990 1254 1135 681 191 1471 Fragments arranged by size: 1471 1254 1135 990 681 219 191 SfoI GGC'GCC Cuts at: 2356 2356 Size: 5941 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5941 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5941 SmaI CCC'GGG Cuts at: 1872 3268 1872 Size: 1396 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2672 3065 4045 4946 5208 820 Size: 197 926 729 393 980 901 262 1553 Fragments arranged by size: 1553 980 926 901 729 393 262 197 SnaBI TAC'GTA Cuts at: 493 3732 493 Size: 3239 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5941 SphI G_CATG'C Cuts at: 1132 3299 1132 Size: 2167 3774 SspI AAT'ATT Cuts at: 5 52 3865 5 Size: 47 3813 2081 Fragments arranged by size: 3813 2081 47 StuI AGG'CCT Cuts at: 1517 2900 2940 1517 Size: 1383 40 4518 Fragments arranged by size: 4518 1383 40 StyI C'CwwG_G Cuts at: 513 1065 2745 2907 3380 513 Size: 552 1680 162 473 3074 Fragments arranged by size: 3074 1680 552 473 162 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2747 3968 3985 4138 4323 4768 325 Size: 1255 1167 1221 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1221 1167 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3078 4187 5899 Size: 274 80 33 51 1443 1101 1109 1712 Cuts at: 5899 96 Size: 138 Fragments arranged by size: 1712 1443 1109 1101 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2042 2074 2726 2758 5901 5933 2042 Size: 32 652 32 3143 32 2050 Fragments arranged by size: 3143 2050 652 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5941 XbaI T'CTAG_A Cuts at: 3277 3277 Size: 5941 XhoI C'TCGA_G Cuts at: 1943 2672 1943 Size: 729 5212 XmaI C'CCGG_G Cuts at: 1870 3266 1870 Size: 1396 4545 XmnI GAAnn'nnTTC Cuts at: 2819 4070 2819 Size: 1251 4690 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2095 3749 276 Size: 53 83 186 936 561 1654 2468 Fragments arranged by size: 2468 1654 936 561 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI EcoNI EcoRV FspAI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SexAI SfiI SrfI SwaI XcmI