Restriction map for
pFN21AB6619
(Linear) (Six-base) MAPSORT of: KIBB6619.seq Check: 6942 from: 1 to: 1236
KIBB6619.orf
With 182 enzymes: SgfI *
October 7, 2009 14:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1038 1236
Size: 1038 198
AatII G_ACGT'C
Cuts at: 0 92 1236
Size: 92 1144
AccI GT'mk_AC
Cuts at: 0 964 1236
Size: 964 272
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 175 1216 1236
Size: 91 84 1041 20
Fragments arranged by size:
1041 91 84 20
AgeI A'CCGG_T
Cuts at: 0 455 967 1236
Size: 455 512 269
Fragments arranged by size:
512 455 269
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1150 1236
Size: 1150 86
AlwNI CAG_nnn'CTG
Cuts at: 0 287 1236
Size: 287 949
ApoI r'AATT_y
Cuts at: 0 445 946 994 1236
Size: 445 501 48 242
Fragments arranged by size:
501 445 242 48
AvaI C'yCGr_G
Cuts at: 0 667 833 1236
Size: 667 166 403
Fragments arranged by size:
667 403 166
BanI G'GyrC_C
Cuts at: 0 268 349 721 859 1236
Size: 268 81 372 138 377
Fragments arranged by size:
377 372 268 138 81
BanII G_rGCy'C
Cuts at: 0 389 749 1236
Size: 389 360 487
Fragments arranged by size:
487 389 360
BbeI G_GCGC'C
Cuts at: 0 353 1236
Size: 353 883
BbsI GAAGACnn'nnnn_
Cuts at: 0 131 631 1236
Size: 131 500 605
Fragments arranged by size:
605 500 131
BglI GCCn_nnn'nGGC
Cuts at: 0 103 1236
Size: 103 1133
BlpI GC'TnA_GC
Cuts at: 0 388 953 1236
Size: 388 565 283
Fragments arranged by size:
565 388 283
Bme1580I G_kGCm'C
Cuts at: 0 271 1236
Size: 271 965
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 453 593 604 1198 1236
Size: 453 140 11 594 38
Fragments arranged by size:
594 453 140 38 11
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1045 1236
Size: 1045 191
BsaI GGTCTCn'nnnn_
Cuts at: 0 619 841 1236
Size: 619 222 395
Fragments arranged by size:
619 395 222
BsaBI GATnn'nnATC
Cuts at: 0 508 1236
Size: 508 728
BsaHI Gr'CG_yC
Cuts at: 0 89 350 1236
Size: 89 261 886
Fragments arranged by size:
886 261 89
BsaWI w'CCGG_w
Cuts at: 0 377 455 967 1236
Size: 377 78 512 269
Fragments arranged by size:
512 377 269 78
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 721 751 1236
Size: 721 30 485
Fragments arranged by size:
721 485 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 223 1129 1236
Size: 223 906 107
Fragments arranged by size:
906 223 107
BseYI C'CCAG_C
Cuts at: 0 317 636 852 1236
Size: 317 319 216 384
Fragments arranged by size:
384 319 317 216
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1066 1156 1236
Size: 1066 90 80
Fragments arranged by size:
1066 90 80
BsiEI CG_ry'CG
Cuts at: 0 1086 1236
Size: 1086 150
BsiHKAI G_wGCw'C
Cuts at: 0 389 749 1236
Size: 389 360 487
Fragments arranged by size:
487 389 360
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1216 1236
Size: 1216 20
Bsp1286I G_dGCh'C
Cuts at: 0 271 389 749 1236
Size: 271 118 360 487
Fragments arranged by size:
487 360 271 118
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1038 1236
Size: 1038 198
BsrDI GCAATG_nn'
Cuts at: 0 92 422 477 1236
Size: 92 330 55 759
Fragments arranged by size:
759 330 92 55
BsrFI r'CCGG_y
Cuts at: 0 222 455 967 1236
Size: 222 233 512 269
Fragments arranged by size:
512 269 233 222
BsrGI T'GTAC_A
Cuts at: 0 1073 1236
Size: 1073 163
BssSI C'ACGA_G
Cuts at: 0 51 61 1236
Size: 51 10 1175
Fragments arranged by size:
1175 51 10
BstEII G'GTnAC_C
Cuts at: 0 702 1236
Size: 702 534
BstZ17I GTA'TAC
Cuts at: 0 965 1236
Size: 965 271
Bsu36I CC'TnA_GG
Cuts at: 0 1189 1236
Size: 1189 47
BtgI C'CryG_G
Cuts at: 0 17 345 532 902 1236
Size: 17 328 187 370 334
Fragments arranged by size:
370 334 328 187 17
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 276 996 1236
Size: 276 720 240
Fragments arranged by size:
720 276 240
BtsI GCAGTG_nn'
Cuts at: 0 1005 1226 1236
Size: 1005 221 10
Fragments arranged by size:
1005 221 10
DraIII CAC_nnn'GTG
Cuts at: 0 53 1236
Size: 53 1183
EaeI y'GGCC_r
Cuts at: 0 104 114 220 224 1083 1236
Size: 104 10 106 4 859 153
Fragments arranged by size:
859 153 106 104 10 4
EagI C'GGCC_G
Cuts at: 0 1083 1236
Size: 1083 153
EarI CTCTTCn'nnn_
Cuts at: 0 754 1236
Size: 754 482
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 991 1236
Size: 991 245
EcoICRI GAG'CTC
Cuts at: 0 387 747 1236
Size: 387 360 489
Fragments arranged by size:
489 387 360
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 175 453 593 604 1198 1216 1236
Size: 91 84 278 140 11 594 18 20
Fragments arranged by size:
594 278 140 91 84 20 18 11
EcoO109I rG'GnC_Cy
Cuts at: 0 284 633 690 1236
Size: 284 349 57 546
Fragments arranged by size:
546 349 284 57
EcoRI G'AATT_C
Cuts at: 0 445 1236
Size: 445 791
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 144 1236
Size: 144 1092
FseI GG_CCGG'CC
Cuts at: 0 226 1236
Size: 226 1010
FspI TGC'GCA
Cuts at: 0 919 1236
Size: 919 317
HaeII r_GCGC'y
Cuts at: 0 353 607 1236
Size: 353 254 629
Fragments arranged by size:
629 353 254
HindIII A'AGCT_T
Cuts at: 0 157 1236
Size: 157 1079
KasI G'GCGC_C
Cuts at: 0 349 1236
Size: 349 887
MscI TGG'CCA
Cuts at: 0 106 116 1236
Size: 106 10 1120
Fragments arranged by size:
1120 106 10
MslI CAynn'nnrTG
Cuts at: 0 61 823 1132 1236
Size: 61 762 309 104
Fragments arranged by size:
762 309 104 61
MspA1I CmG'CkG
Cuts at: 0 321 392 1236
Size: 321 71 844
Fragments arranged by size:
844 321 71
NaeI GCC'GGC
Cuts at: 0 224 1236
Size: 224 1012
NarI GG'CG_CC
Cuts at: 0 350 1236
Size: 350 886
NcoI C'CATG_G
Cuts at: 0 902 1236
Size: 902 334
NgoMIV G'CCGG_C
Cuts at: 0 222 1236
Size: 222 1014
PfoI T'CCnGG_A
Cuts at: 0 581 1236
Size: 581 655
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 661 693 1236
Size: 661 32 543
Fragments arranged by size:
661 543 32
PpuMI rG'GwC_Cy
Cuts at: 0 633 690 1236
Size: 633 57 546
Fragments arranged by size:
633 546 57
PshAI GACnn'nnGTC
Cuts at: 0 709 1236
Size: 709 527
PvuII CAG'CTG
Cuts at: 0 392 1236
Size: 392 844
SacI G_AGCT'C
Cuts at: 0 389 749 1236
Size: 389 360 487
Fragments arranged by size:
487 389 360
SanDI GG'GwC_CC
Cuts at: 0 690 1236
Size: 690 546
SapI GCTCTTCn'nnn_
Cuts at: 0 754 1236
Size: 754 482
SfcI C'TryA_G
Cuts at: 0 30 1236
Size: 30 1206
SfoI GGC'GCC
Cuts at: 0 351 1236
Size: 351 885
SmlI C'TyrA_G
Cuts at: 0 667 1060 1236
Size: 667 393 176
Fragments arranged by size:
667 393 176
StuI AGG'CCT
Cuts at: 0 895 935 1236
Size: 895 40 301
Fragments arranged by size:
895 301 40
StyI C'CwwG_G
Cuts at: 0 740 902 1236
Size: 740 162 334
Fragments arranged by size:
740 334 162
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 742 1236
Size: 742 494
TatI w'GTAC_w
Cuts at: 0 1073 1236
Size: 1073 163
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 37 69 721 753 1236
Size: 37 32 652 32 483
Fragments arranged by size:
652 483 37 32 32
XhoI C'TCGA_G
Cuts at: 0 667 1236
Size: 667 569
XmnI GAAnn'nnTTC
Cuts at: 0 814 1236
Size: 814 422
ZraI GAC'GTC
Cuts at: 0 90 1236
Size: 90 1146
Enzymes that do cut:
AarI AatII AccI AcuI AgeI AlfI AlwNI ApoI
AvaI BanI BanII BbeI BbsI BglI BlpI Bme1580I
BpmI BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrDI
BsrFI BsrGI BssSI BstEII BstZ17I Bsu36I BtgI BtgZI
BtsI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FseI FspI HaeII HindIII KasI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV PfoI
PpiI PpuMI PshAI PvuII SacI SanDI SapI SfcI
SfoI SmlI StuI StyI TaqII TatI TstI XhoI
XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AflIII AhdI AleI AloI
ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BbvCI BcgI BciVI BclI BfrBI BglII BmgBI BmrI
BmtI BplI Bpu10I BsaAI BsiWI BsmI BspEI BspHI
BsrBI BssHII BstAPI BstBI BstXI BstYI ClaI CspCI
DraI DrdI EcoNI EcoRV FspAI Hin4I HincII HpaI
KpnI MfeI MluI MmeI NdeI NheI NotI NruI
NsiI NspI PacI PasI PciI PflMI PmeI PmlI
PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacII
SalI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI SwaI Tth111I XbaI
XcmI XmaI