Restriction map for
pFN21AB6615
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# NO CUT ENZYMES ( 140 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BamHI BbeI BbrPI BbvCI
BcgI BciVI BclI BfrI BglII
BlnI BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsiWI BsmAI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrGI Bst1107I
BstBI BstXI BstYI BstZ17I Bsu36I
ClaI CpoI DpnII DraI DraIII
DrdI Eam1105I Eco47III Eco81I EcoRI
EcoRV EcoT22I FbaI FspI Hin1I
HincII HindII HindIII HinfI HpaI
KasI KpnI MamI MboI MfeI
MflI MluI MroI MseI MunI
NarI NcoI NdeI NdeII NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PleI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SalI
SapI Sau3AI ScaI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
SspBI SspI SwaI TaqI TfiI
Tru9I Tsp509I Tth111I TthHB8I XbaI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 33 enzymes) #
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AspHI 664 GTGCTC G[AT]GC[AT]^C
BbsI 901 GTCTTC GAAGAC(2/6)
BlpI 966 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 966 GCTGAGC GC^T[ACGT]AGC
BpuAI 901 GTCTTC GAAGAC(2/6)
BseRI 795 CTCCTC GAGGAG(10/8)
BsiEI 386 CGGCCG CG[GA][CT]^CG
BsiHKAI 664 GTGCTC G[AT]GC[AT]^C
BspMI 414 GCAGGT ACCTGC(4/8)
BssHII 455 GCGCGC G^CGCGC
BssSI 483 CTCGTG CACGAG(-5/-1)
BstEII 1002 GGTCACC G^GT[ACGT]ACC
BstPI 1002 GGTCACC G^GT[ACGT]ACC
CelII 966 GCTGAGC GC^T[ACGT]AGC
DdeI 967 CTGAG C^T[ACGT]AG
EagI 386 CGGCCG C^GGCCG
EarI 710 CTCTTC CTCTTC(1/4)
EclXI 386 CGGCCG C^GGCCG
Eco52I 386 CGGCCG C^GGCCG
EcoNI 36 CCTGGTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1002 GGTCACC G^GT[ACGT]ACC
FseI 24 GGCCGGCC GGCCGG^CC
HaeII 465 GGCGCT [GA]GCGC^[CT]
Ksp632I 710 CTCTTC CTCTTC(1/4)
KspI 530 CCGCGG CCGC^GG
MaeII 623 ACGT A^CGT
MslI 1020 CACTGGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PpuMI 808 GGGACCT [GA]G^G[AT]CC[CT]
SacII 530 CCGCGG CCGC^GG
SbfI 1059 CCTGCAGG CCTGCA^GG
SexAI 35 ACCTGGT A^CC[AT]GGT
SfaNI 1029 GATGC GCATC(5/9)
Sse8387I 1059 CCTGCAGG CCTGCA^GG
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# TWO CUT ENZYMES ( 18 enzymes) #
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AlwNI 43 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG
373 CAGCTGCTG
ApaI 167 GGGCCC GGGCC^C
1055 GGGCCC
AvaI 742 CCCGAG C^[CT]CG[GA]G
871 CTCGGG
BanI 331 GGTGCC G^G[CT][GA]CC
691 GGTGCC
BsoBI 742 CCCGAG C^[CT]CG[GA]G
871 CTCGGG
BstAPI 42 GCAGGGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
609 GCACCTTCTGC
EcoT14I 128 CCAAGG C^C[AT][AT]GG
896 CCTTGG
MboII 711 TCTTC GAAGA(8/7)
902 TCTTC
NheI 444 GCTAGC G^CTAGC
627 GCTAGC
NlaIII 339 CATG CATG^
930 CATG
PflMI 515 CCACCTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
821 CCAAGCACTGG
PvuII 373 CAGCTG CAG^CTG
1042 CAGCTG
SfiI 1047 GGCCCCCAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1056 GGCCCTGCAGGCC
SmlI 154 CTCAAG C^T[CT][GA]AG
948 CTTGAG
StuI 996 AGGCCT AGG^CCT
1134 AGGCCT
StyI 128 CCAAGG C^C[AT][AT]GG
896 CCTTGG
Van91I 515 CCACCTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
821 CCAAGCACTGG
XcmI 338 CCATGCAGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
515 CCACCTACTGGCTGG
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# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AccII AciI AfaI AluI AvaII
BalI BanII BbvI BcnI BfaI
BglI BmyI BpmI BsaJI BsgI
BsiYI BslI BsmFI Bsp1286I BsrFI
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DraII
DsaI EaeI EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HpaII HphI ItaI
MaeI MaeIII MluNI MnlI MscI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NgoMIV NlaIV PstI
RsaI Sau96I ScrFI SfcI TseI
Tsp45I TspRI