(Circular) (Six-base) MAPSORT of: pFN21AB6615.seq Check: 3536 from: 1 to: 5941 pFN21AB6615 5941 bp With 182 enzymes: SgfI * October 20, 2009 10:57 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2410 2410 Size: 5941 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3751 278 Size: 53 83 186 936 2215 2468 Fragments arranged by size: 2468 2215 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3284 1937 Size: 1347 4594 Acc65I G'GTAC_C Cuts at: 3262 3262 Size: 5941 AclI AA'CG_TT Cuts at: 1563 4068 4441 1563 Size: 2505 373 3063 Fragments arranged by size: 3063 2505 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4004 5388 5656 1632 Size: 2372 1384 268 1917 Fragments arranged by size: 2372 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5744 AflIII A'CryG_T Cuts at: 1203 4840 5817 1203 Size: 3637 977 1327 Fragments arranged by size: 3637 1327 977 AgeI A'CCGG_T Cuts at: 4772 4772 Size: 5941 AhdI GACnn_n'nnGTC Cuts at: 4670 4670 Size: 5941 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5941 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2432 1184 Size: 1248 4693 AlwNI CAG_nnn'CTG Cuts at: 2053 2383 3684 5256 2053 Size: 330 1301 1572 2738 Fragments arranged by size: 2738 1572 1301 330 ApaI G_GGCC'C Cuts at: 2176 3064 2176 Size: 888 5053 ApaLI G'TGCA_C Cuts at: 3998 5154 3998 Size: 1156 4785 ApoI r'AATT_y Cuts at: 1493 3250 3476 3628 5737 1493 Size: 1757 226 152 2109 1697 Fragments arranged by size: 2109 1757 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4495 160 Size: 872 3463 1606 Fragments arranged by size: 3463 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5941 AvaI C'yCGr_G Cuts at: 1870 1943 2747 2876 3266 1870 Size: 73 804 129 390 4545 Fragments arranged by size: 4545 804 390 129 73 BamHI G'GATC_C Cuts at: 3271 3638 3271 Size: 367 5574 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2336 2696 3262 4717 618 Size: 325 217 627 549 360 566 1455 1842 Fragments arranged by size: 1842 1455 627 566 549 360 325 217 BanII G_rGCy'C Cuts at: 1391 2067 2176 2198 3064 3260 1391 Size: 676 109 22 866 196 4072 Fragments arranged by size: 4072 866 676 196 109 22 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2899 928 Size: 971 1000 3970 Fragments arranged by size: 3970 1000 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3220 3254 4132 4166 1903 Size: 34 50 34 1199 34 878 34 3678 Fragments arranged by size: 3678 1199 878 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3833 5043 3833 Size: 1210 4731 BclI T'GATC_A Cuts at: 1548 1548 Size: 5941 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2062 2306 2880 Size: 107 122 71 631 605 390 244 574 Cuts at: 2880 3059 3068 4552 136 Size: 179 9 1484 1525 Fragments arranged by size: 1525 1484 631 605 574 390 244 179 122 107 71 9 BglII A'GATC_T Cuts at: 5936 5936 Size: 5941 BlpI GC'TnA_GC Cuts at: 2972 3358 2972 Size: 386 5555 Bme1580I G_kGCm'C Cuts at: 1790 2176 3064 3128 4002 5158 1790 Size: 386 888 64 874 1156 2573 Fragments arranged by size: 2573 1156 888 874 386 64 BmgBI CAC'GTC Cuts at: 1318 2629 1318 Size: 1311 4630 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2408 3036 3672 Size: 416 53 83 399 24 991 628 636 Cuts at: 3672 4630 442 Size: 958 1753 Fragments arranged by size: 1753 991 958 636 628 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2453 2636 1056 Size: 1397 183 4361 Fragments arranged by size: 4361 1397 183 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2051 2295 2405 3119 4601 1239 Size: 812 244 110 714 1482 2579 Fragments arranged by size: 2579 1482 812 714 244 110 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2144 2974 4066 4931 5229 2144 Size: 830 1092 865 298 2856 Fragments arranged by size: 2856 1092 865 830 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4604 882 Size: 617 3105 2219 Fragments arranged by size: 3105 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3732 493 Size: 711 2528 2702 Fragments arranged by size: 2702 2528 711 BsaBI GATnn'nnATC Cuts at: 5935 5935 Size: 5941 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3748 4130 4765 275 Size: 53 83 186 936 2215 382 635 1451 Fragments arranged by size: 2215 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4374 4772 5046 5193 5604 4374 Size: 398 274 147 411 4711 Fragments arranged by size: 4711 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2991 3021 1178 Size: 30 165 30 1588 30 4098 Fragments arranged by size: 4098 1588 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2791 1186 Size: 1605 4336 BseYI C'CCAG_C Cuts at: 2437 2453 2683 3106 3121 3227 5144 5640 2437 Size: 16 230 423 15 106 1917 496 2738 Fragments arranged by size: 2738 1917 496 423 230 106 16 15 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2066 2396 2438 2538 1163 Size: 573 330 330 42 100 4566 Fragments arranged by size: 4566 573 330 330 100 42 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2394 3391 4152 5180 1540 Size: 275 186 393 997 761 1028 2301 Fragments arranged by size: 2301 1028 997 761 393 275 186 BsiHKAI G_wGCw'C Cuts at: 2673 4002 4087 5158 2673 Size: 1329 85 1071 3456 Fragments arranged by size: 3456 1329 1071 85 BsmI GAATG_Cn' Cuts at: 3452 3545 3452 Size: 93 5848 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2067 2176 2198 2673 3064 3128 3260 Size: 399 277 109 22 475 391 64 132 Cuts at: 3260 4002 4087 5158 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 475 399 391 277 132 109 85 64 22 BspEI T'CCGG_A Cuts at: 5604 5604 Size: 5941 BspHI T'CATG_A Cuts at: 3830 5560 3830 Size: 1730 4211 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2410 3296 844 Size: 1566 886 3489 Fragments arranged by size: 3489 1566 886 BsrBI CCG'CTC Cuts at: 1394 1823 3828 1394 Size: 429 2005 3507 Fragments arranged by size: 3507 2005 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4436 4610 65 Size: 1183 3188 174 1396 Fragments arranged by size: 3188 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2030 2099 2389 4585 4772 1700 Size: 110 220 69 290 2196 187 2869 Fragments arranged by size: 2869 2196 290 220 187 110 69 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5941 BssHII G'CGCG_C Cuts at: 1925 2460 1925 Size: 535 5406 BssSI C'ACGA_G Cuts at: 2488 4001 5013 2488 Size: 1513 1012 3416 Fragments arranged by size: 3416 1513 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2053 2620 794 Size: 1259 567 4115 Fragments arranged by size: 4115 1259 567 BstBI TT'CG_AA Cuts at: 4745 4745 Size: 5941 BstEII G'GTnAC_C Cuts at: 1184 3007 1184 Size: 1823 4118 BstYI r'GATC_y Cuts at: 1972 3271 3638 4023 4040 5492 5578 5590 5936 Size: 1299 367 385 17 1452 86 12 346 Cuts at: 5936 1972 Size: 1977 Fragments arranged by size: 1977 1452 1299 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2150 2535 2731 513 Size: 552 1085 385 196 3723 Fragments arranged by size: 3723 1085 552 385 196 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2776 487 Size: 683 56 1550 3652 Fragments arranged by size: 3652 1550 683 56 BtsI GCAGTG_nn' Cuts at: 3457 4251 4271 3457 Size: 794 20 5127 Fragments arranged by size: 5127 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5941 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5906 DraI TTT'AAA Cuts at: 3245 3245 Size: 5941 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4948 809 Size: 507 3632 1802 Fragments arranged by size: 3632 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2028 2130 2281 Size: 54 1425 50 275 18 198 102 151 Cuts at: 2281 2391 2492 2532 2823 3090 3132 3388 4277 Size: 110 101 40 291 267 42 256 889 Cuts at: 4277 5906 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 291 275 267 256 198 151 110 102 101 54 50 43 42 40 18 EagI C'GGCC_G Cuts at: 1537 1812 2391 3388 1537 Size: 275 579 997 4090 Fragments arranged by size: 4090 997 579 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2721 3871 1353 Size: 1368 1150 3423 Fragments arranged by size: 3423 1368 1150 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2861 4528 4902 5048 387 Size: 1064 138 1272 1667 374 146 1280 Fragments arranged by size: 1667 1280 1272 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2051 2295 2405 3119 4004 4601 5388 Size: 393 419 244 110 714 885 597 787 Cuts at: 5388 5656 1239 Size: 268 1524 Fragments arranged by size: 1524 885 787 714 597 419 393 268 244 110 EcoNI CCTnn'n_nnAGG Cuts at: 2045 2045 Size: 5941 EcoO109I rG'GnC_Cy Cuts at: 2050 2173 2205 2814 2881 3060 3061 2050 Size: 123 32 609 67 179 1 4930 Fragments arranged by size: 4930 609 179 123 67 32 1 EcoRI G'AATT_C Cuts at: 3250 3628 3250 Size: 378 5563 FseI GG_CCGG'CC Cuts at: 2034 2034 Size: 5941 FspI TGC'GCA Cuts at: 4447 4447 Size: 5941 HaeII r_GCGC'y Cuts at: 1678 2474 5088 1678 Size: 796 2614 2531 Fragments arranged by size: 2614 2531 796 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2965 2991 2997 3023 4582 Size: 32 135 32 1393 26 6 26 1559 Cuts at: 4582 4614 4656 4688 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1559 1393 135 42 32 32 32 32 26 26 6 HincII GTy'rAC Cuts at: 669 1657 1938 3285 3531 669 Size: 988 281 1347 246 3079 Fragments arranged by size: 3079 1347 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5941 HpaI GTT'AAC Cuts at: 3531 3531 Size: 5941 KpnI G_GTAC'C Cuts at: 3266 3266 Size: 5941 MfeI C'AATT_G Cuts at: 3540 3540 Size: 5941 MluI A'CGCG_T Cuts at: 5817 5817 Size: 5941 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5055 5239 1305 Size: 3750 184 2007 Fragments arranged by size: 3750 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2132 2283 2825 3092 10 Size: 54 1425 343 300 151 542 267 2859 Fragments arranged by size: 2859 1425 542 343 300 267 151 54 MslI CAynn'nnrTG Cuts at: 518 1166 3029 3899 4258 4417 518 Size: 648 1863 870 359 159 2042 Fragments arranged by size: 2042 1863 870 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2026 2179 2303 2380 2537 3049 3357 4034 Size: 420 153 124 77 157 512 308 677 Cuts at: 4034 5182 5427 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 512 420 308 245 157 153 124 77 NaeI GCC'GGC Cuts at: 2032 2101 2391 2032 Size: 69 290 5582 Fragments arranged by size: 5582 290 69 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5389 NdeI CA'TA_TG Cuts at: 387 387 Size: 5941 NgoMIV G'CCGG_C Cuts at: 2030 2099 2389 2030 Size: 69 290 5582 Fragments arranged by size: 5582 290 69 NheI G'CTAG_C Cuts at: 1052 2449 2632 1052 Size: 1397 183 4361 Fragments arranged by size: 4361 1397 183 NotI GC'GGCC_GC Cuts at: 3388 3388 Size: 5941 NspI r_CATG'y Cuts at: 1132 1229 3299 4844 1132 Size: 97 2070 1545 2229 Fragments arranged by size: 2229 2070 1545 97 PasI CC'CwG_GG Cuts at: 1348 2806 2923 3057 1348 Size: 1458 117 134 4232 Fragments arranged by size: 4232 1458 134 117 PciI A'CATG_T Cuts at: 4840 4840 Size: 5941 PflMI CCAn_nnn'nTGG Cuts at: 1108 2526 2832 1108 Size: 1418 306 4217 Fragments arranged by size: 4217 1418 306 PfoI T'CCnGG_A Cuts at: 2272 2272 Size: 5941 PmeI GTTT'AAAC Cuts at: 3245 3245 Size: 5941 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4010 4042 5523 5555 4010 Size: 32 1481 32 4396 Fragments arranged by size: 4396 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2814 2814 Size: 5941 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5941 PsiI TTA'TAA Cuts at: 3511 3511 Size: 5941 PspOMI G'GGCC_C Cuts at: 2172 3060 2172 Size: 888 5053 PstI C_TGCA'G Cuts at: 830 2988 3044 3069 3293 830 Size: 2158 56 25 224 3478 Fragments arranged by size: 3478 2158 224 56 25 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5941 PvuII CAG'CTG Cuts at: 2380 3049 2380 Size: 669 5272 SacII CC_GC'GG Cuts at: 2538 2538 Size: 5941 SalI G'TCGA_C Cuts at: 1936 3283 1936 Size: 1347 4594 SbfI CC_TGCA'GG Cuts at: 3069 3293 3069 Size: 224 5717 ScaI AGT'ACT Cuts at: 4189 5901 4189 Size: 1712 4229 SexAI A'CCwGG_T Cuts at: 2040 2040 Size: 5941 SfcI C'TryA_G Cuts at: 826 1045 2984 3040 3065 3079 3289 4424 5105 Size: 219 1939 56 25 14 210 1135 681 Cuts at: 5105 5296 826 Size: 191 1471 Fragments arranged by size: 1939 1471 1135 681 219 210 191 56 25 14 SfiI GGCCn_nnn'nGGCC Cuts at: 3059 3068 3059 Size: 9 5932 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5941 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5941 SmaI CCC'GGG Cuts at: 1872 3268 1872 Size: 1396 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2159 2953 4045 4946 5208 820 Size: 197 926 216 794 1092 901 262 1553 Fragments arranged by size: 1553 1092 926 901 794 262 216 197 SnaBI TAC'GTA Cuts at: 493 3732 493 Size: 3239 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5941 SphI G_CATG'C Cuts at: 1132 3299 1132 Size: 2167 3774 SspI AAT'ATT Cuts at: 5 52 3865 5 Size: 47 3813 2081 Fragments arranged by size: 3813 2081 47 StuI AGG'CCT Cuts at: 1517 3003 3141 1517 Size: 1486 138 4317 Fragments arranged by size: 4317 1486 138 StyI C'CwwG_G Cuts at: 513 1065 2133 2901 3380 513 Size: 552 1068 768 479 3074 Fragments arranged by size: 3074 1068 768 552 479 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2200 2324 2675 2762 2798 3241 3968 Size: 1255 620 124 351 87 36 443 727 Cuts at: 3968 3985 4138 4323 4768 325 Size: 17 153 185 445 1498 Fragments arranged by size: 1498 1255 727 620 445 443 351 185 153 124 87 36 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4187 5899 96 Size: 274 80 33 51 1443 2210 1712 138 Fragments arranged by size: 2210 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5901 5933 5901 Size: 32 5909 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5941 XbaI T'CTAG_A Cuts at: 3277 3277 Size: 5941 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2350 2527 2350 Size: 177 5764 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5941 XmaI C'CCGG_G Cuts at: 1870 3266 1870 Size: 1396 4545 XmnI GAAnn'nnTTC Cuts at: 4070 4070 Size: 5941 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3749 276 Size: 53 83 186 936 2215 2468 Fragments arranged by size: 2468 2215 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SanDI SapI SfoI SrfI SwaI