(Circular) (Six-base) MAPSORT of: pFN21AB6612.seq Check: 3356 from: 1 to: 6001 pFN21AB6612 6001 bp With 182 enzymes: SgfI * October 20, 2009 10:57 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3811 278 Size: 53 83 186 936 2275 2468 Fragments arranged by size: 2468 2275 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3344 1937 Size: 1407 4594 Acc65I G'GTAC_C Cuts at: 3322 3322 Size: 6001 AclI AA'CG_TT Cuts at: 1563 4128 4501 1563 Size: 2565 373 3063 Fragments arranged by size: 3063 2565 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2169 2679 2801 4064 5448 5716 1632 Size: 537 510 122 1263 1384 268 1917 Fragments arranged by size: 1917 1384 1263 537 510 268 122 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5804 AflIII A'CryG_T Cuts at: 1203 4900 5877 1203 Size: 3697 977 1327 Fragments arranged by size: 3697 1327 977 AgeI A'CCGG_T Cuts at: 4832 4832 Size: 6001 AhdI GACnn_n'nnGTC Cuts at: 4730 4730 Size: 6001 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6001 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6001 AlwNI CAG_nnn'CTG Cuts at: 2473 3158 3744 5316 2473 Size: 685 586 1572 3158 Fragments arranged by size: 3158 1572 685 586 ApaI G_GGCC'C Cuts at: 2013 2490 2652 2013 Size: 477 162 5362 Fragments arranged by size: 5362 477 162 ApaLI G'TGCA_C Cuts at: 4058 5214 4058 Size: 1156 4845 ApoI r'AATT_y Cuts at: 1493 2746 3310 3536 3688 5797 1493 Size: 1253 564 226 152 2109 1697 Fragments arranged by size: 2109 1697 1253 564 226 152 AseI AT'TA_AT Cuts at: 160 1032 4555 160 Size: 872 3523 1606 Fragments arranged by size: 3523 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6001 AvaI C'yCGr_G Cuts at: 1870 1943 2417 2907 3326 1870 Size: 73 474 490 419 4545 Fragments arranged by size: 4545 490 474 419 73 BamHI G'GATC_C Cuts at: 3331 3698 3331 Size: 367 5634 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3322 4777 618 Size: 325 217 627 1535 1455 1842 Fragments arranged by size: 1842 1535 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2013 2490 2643 2652 3320 1391 Size: 622 477 153 9 668 4072 Fragments arranged by size: 4072 668 622 477 153 9 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2922 928 Size: 971 1023 4007 Fragments arranged by size: 4007 1023 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4192 4226 1903 Size: 34 2255 34 3678 Fragments arranged by size: 3678 2255 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2425 2747 3893 5103 2425 Size: 322 1146 1210 3323 Fragments arranged by size: 3323 1210 1146 322 BclI T'GATC_A Cuts at: 1548 1548 Size: 6001 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2028 2607 4612 Size: 107 122 71 631 605 356 579 2005 Cuts at: 4612 136 Size: 1525 Fragments arranged by size: 2005 1525 631 605 579 356 122 107 71 BglII A'GATC_T Cuts at: 2940 5996 2940 Size: 3056 2945 BlpI GC'TnA_GC Cuts at: 3418 3418 Size: 6001 Bme1580I G_kGCm'C Cuts at: 1790 2013 2263 2490 2652 4062 5218 1790 Size: 223 250 227 162 1410 1156 2573 Fragments arranged by size: 2573 1410 1156 250 227 223 162 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6001 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3732 4690 442 Size: 416 53 83 399 24 2315 958 1753 Fragments arranged by size: 2315 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6001 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2612 4661 1239 Size: 1373 2049 2579 Fragments arranged by size: 2579 2049 1373 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2486 4126 4991 5289 2486 Size: 1640 865 298 3198 Fragments arranged by size: 3198 1640 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3236 4664 882 Size: 617 1737 1428 2219 Fragments arranged by size: 2219 1737 1428 617 BsaAI yAC'GTr Cuts at: 493 1204 3792 493 Size: 711 2588 2702 Fragments arranged by size: 2702 2588 711 BsaBI GATnn'nnATC Cuts at: 2045 5995 2045 Size: 3950 2051 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3808 4190 4825 275 Size: 53 83 186 936 2275 382 635 1451 Fragments arranged by size: 2275 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3013 4434 4832 5106 5253 5664 3013 Size: 1421 398 274 147 411 3350 Fragments arranged by size: 3350 1421 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2218 2248 2498 2528 1178 Size: 30 165 30 815 30 250 30 4651 Fragments arranged by size: 4651 815 250 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2503 2780 3052 3161 1186 Size: 1317 277 272 109 4026 Fragments arranged by size: 4026 1317 277 272 109 BseYI C'CCAG_C Cuts at: 2026 2473 3140 5204 5700 2026 Size: 447 667 2064 496 2327 Fragments arranged by size: 2327 2064 667 496 447 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2568 1163 Size: 573 832 4596 Fragments arranged by size: 4596 832 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3451 4212 5240 1540 Size: 275 186 1450 761 1028 2301 Fragments arranged by size: 2301 1450 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2117 2502 2643 4062 4147 5218 2117 Size: 385 141 1419 85 1071 2900 Fragments arranged by size: 2900 1419 1071 385 141 85 BsmI GAATG_Cn' Cuts at: 3512 3605 3512 Size: 93 5908 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2013 2117 2263 2490 2502 2643 2652 Size: 399 223 104 146 227 12 141 9 Cuts at: 2652 3320 4062 4147 5218 1391 Size: 668 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 668 399 227 223 146 141 104 85 12 9 BspEI T'CCGG_A Cuts at: 3013 5664 3013 Size: 2651 3350 BspHI T'CATG_A Cuts at: 3890 5620 3890 Size: 1730 4271 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2312 3356 844 Size: 1468 1044 3489 Fragments arranged by size: 3489 1468 1044 BsrBI CCG'CTC Cuts at: 1394 1823 3888 1394 Size: 429 2065 3507 Fragments arranged by size: 3507 2065 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4496 4670 65 Size: 1183 3248 174 1396 Fragments arranged by size: 3248 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4645 4832 1700 Size: 110 2835 187 2869 Fragments arranged by size: 2869 2835 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6001 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6001 BssSI C'ACGA_G Cuts at: 4061 5073 4061 Size: 1012 4989 BstAPI GCAn_nnn'nTGC Cuts at: 794 2231 794 Size: 1437 4564 BstBI TT'CG_AA Cuts at: 3099 4805 3099 Size: 1706 4295 BstEII G'GTnAC_C Cuts at: 1184 3054 1184 Size: 1870 4131 BstYI r'GATC_y Cuts at: 1972 2344 2940 3331 3698 4083 4100 5552 5638 Size: 372 596 391 367 385 17 1452 86 Cuts at: 5638 5650 5996 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 596 391 385 372 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2013 3226 513 Size: 552 939 9 1213 3288 Fragments arranged by size: 3288 1213 939 552 9 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5262 Fragments arranged by size: 5262 683 56 BtsI GCAGTG_nn' Cuts at: 2251 2433 3517 4311 4331 2251 Size: 182 1084 794 20 3921 Fragments arranged by size: 3921 1084 794 182 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6001 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5966 DraI TTT'AAA Cuts at: 3305 3305 Size: 6001 DraIII CAC_nnn'GTG Cuts at: 2055 2055 Size: 6001 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5008 809 Size: 507 3692 1802 Fragments arranged by size: 3692 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3220 3448 4337 Size: 54 1425 50 275 18 1390 228 889 Cuts at: 4337 5966 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1390 889 275 228 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3448 1537 Size: 275 1636 4090 Fragments arranged by size: 4090 1636 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2757 2838 2931 3931 1353 Size: 1404 81 93 1000 3423 Fragments arranged by size: 3423 1404 1000 93 81 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2855 4588 4962 5108 387 Size: 1064 138 1266 1733 374 146 1280 Fragments arranged by size: 1733 1280 1266 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2641 2641 Size: 6001 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2169 2612 2679 2801 4064 4661 5448 Size: 393 537 443 67 122 1263 597 787 Cuts at: 5448 5716 1239 Size: 268 1524 Fragments arranged by size: 1524 1263 787 597 537 443 393 268 122 67 EcoO109I rG'GnC_Cy Cuts at: 2009 2486 2487 2648 2649 2770 3043 2009 Size: 477 1 161 1 121 273 4967 Fragments arranged by size: 4967 477 273 161 121 1 1 EcoRI G'AATT_C Cuts at: 3310 3688 3310 Size: 378 5623 FspI TGC'GCA Cuts at: 4507 4507 Size: 6001 HaeII r_GCGC'y Cuts at: 1678 5148 1678 Size: 3470 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2498 2530 3163 3195 4642 Size: 32 135 32 926 32 633 32 1447 Cuts at: 4642 4674 4716 4748 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1447 926 633 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3345 3591 669 Size: 988 281 1407 246 3079 Fragments arranged by size: 3079 1407 988 281 246 HindIII A'AGCT_T Cuts at: 748 2792 748 Size: 2044 3957 HpaI GTT'AAC Cuts at: 3591 3591 Size: 6001 KpnI G_GTAC'C Cuts at: 3326 3326 Size: 6001 MfeI C'AATT_G Cuts at: 3600 3600 Size: 6001 MluI A'CGCG_T Cuts at: 5877 5877 Size: 6001 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5115 5299 1305 Size: 3810 184 2007 Fragments arranged by size: 3810 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3222 10 Size: 54 1425 343 1390 2789 Fragments arranged by size: 2789 1425 1390 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3959 4318 4477 518 Size: 648 2793 359 159 2042 Fragments arranged by size: 2793 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2015 2034 2582 3417 4094 5242 5487 1606 Size: 409 19 548 835 677 1148 245 2120 Fragments arranged by size: 2120 1148 835 677 548 409 245 19 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4510 Fragments arranged by size: 4510 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6001 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6001 NotI GC'GGCC_GC Cuts at: 3448 3448 Size: 6001 NspI r_CATG'y Cuts at: 1132 1229 2311 3359 4904 1132 Size: 97 1082 1048 1545 2229 Fragments arranged by size: 2229 1545 1082 1048 97 PasI CC'CwG_GG Cuts at: 1348 2645 3208 1348 Size: 1297 563 4141 Fragments arranged by size: 4141 1297 563 PciI A'CATG_T Cuts at: 4900 4900 Size: 6001 PflMI CCAn_nnn'nTGG Cuts at: 1108 2473 1108 Size: 1365 4636 PmeI GTTT'AAAC Cuts at: 3305 3305 Size: 6001 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4070 4102 5583 5615 4070 Size: 32 1481 32 4456 Fragments arranged by size: 4456 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3043 3043 Size: 6001 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6001 PsiI TTA'TAA Cuts at: 3571 3571 Size: 6001 PspOMI G'GGCC_C Cuts at: 2009 2486 2648 2009 Size: 477 162 5362 Fragments arranged by size: 5362 477 162 PstI C_TGCA'G Cuts at: 830 2581 3353 830 Size: 1751 772 3478 Fragments arranged by size: 3478 1751 772 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6001 PvuII CAG'CTG Cuts at: 2582 2582 Size: 6001 SacI G_AGCT'C Cuts at: 2643 2643 Size: 6001 SacII CC_GC'GG Cuts at: 2016 2016 Size: 6001 SalI G'TCGA_C Cuts at: 1936 3343 1936 Size: 1407 4594 SanDI GG'GwC_CC Cuts at: 3043 3043 Size: 6001 SbfI CC_TGCA'GG Cuts at: 3353 3353 Size: 6001 ScaI AGT'ACT Cuts at: 2296 4249 5961 2296 Size: 1953 1712 2336 Fragments arranged by size: 2336 1953 1712 SfcI C'TryA_G Cuts at: 826 1045 2577 3349 4484 5165 5356 826 Size: 219 1532 772 1135 681 191 1471 Fragments arranged by size: 1532 1471 1135 772 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6001 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6001 SmaI CCC'GGG Cuts at: 1872 3328 1872 Size: 1456 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2501 4105 5006 5268 820 Size: 197 926 558 1604 901 262 1553 Fragments arranged by size: 1604 1553 926 901 558 262 197 SnaBI TAC'GTA Cuts at: 493 3792 493 Size: 3299 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6001 SphI G_CATG'C Cuts at: 1132 2311 3359 1132 Size: 1179 1048 3774 Fragments arranged by size: 3774 1179 1048 SspI AAT'ATT Cuts at: 5 52 3925 5 Size: 47 3873 2081 Fragments arranged by size: 3873 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6001 StyI C'CwwG_G Cuts at: 513 1065 2004 2740 3440 513 Size: 552 939 736 700 3074 Fragments arranged by size: 3074 939 736 700 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4028 4045 4198 4383 4828 325 Size: 1255 2448 17 153 185 445 1498 Fragments arranged by size: 2448 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2294 2952 4247 Size: 274 80 33 51 1443 317 658 1295 Cuts at: 4247 5959 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1295 658 317 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2766 2798 2967 2999 5961 5993 2766 Size: 32 169 32 2962 32 2774 Fragments arranged by size: 2962 2774 169 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6001 XbaI T'CTAG_A Cuts at: 3337 3337 Size: 6001 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6001 XmaI C'CCGG_G Cuts at: 1870 3326 1870 Size: 1456 4545 XmnI GAAnn'nnTTC Cuts at: 2814 4130 2814 Size: 1316 4685 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3809 276 Size: 53 83 186 936 2275 2468 Fragments arranged by size: 2468 2275 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SapI SexAI SfiI SfoI SrfI SwaI XcmI