Restriction map for
pFN21AB6136
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# NO CUT ENZYMES ( 106 enzymes) #
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AatII AccI AccIII AclI AflII
AflIII AgeI Alw44I Aor51HI ApaLI
AscI AseI AsnI Asp700I AspI
AviII AvrII BbrPI BbsI BfrI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaI BseAI BsiWI BsmBI
Bsp1407I BspDI BspEI BspLU11I BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstZ17I CelII Cfr10I
ClaI CpoI DraI DraIII EagI
EclXI Eco47III Eco52I EcoO65I EcoRV
EcoT14I EcoT22I FseI FspI HpaI
KspI MfeI MluI MroI MseI
MslI MunI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I RsrII SacII SalI ScaI
SexAI SfiI SfuI SgrAI SnaBI
SpeI SphI SspBI StyI SwaI
TfiI Tru9I Tth111I Van91I XbaI
XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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BamHI 1092 GGATCC G^GATCC
BcgI 1697 GCATCAACCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1370 GTATCC GTATCC(6/5)
BclI 999 TGATCA T^GATCA
BfaI 1582 CTAG C^TAG
BsaBI 556 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 543 CGATCG CG[GA][CT]^CG
BspHI 50 TCATGA T^CATGA
BsrBI 46 CCGCTC CCGCTC(-3/-3)
BstXI 152 CCAAGCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI 411 GACTGACTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1520 CCGTGG C^C[GA][CT]GG
EarI 1420 CTCTTC CTCTTC(1/4)
FbaI 999 TGATCA T^GATCA
HgaI 1870 GACGC GACGC(5/10)
HincII 772 GTTGAC GT[CT]^[GA]AC
HindII 772 GTTGAC GT[CT]^[GA]AC
HindIII 1109 AAGCTT A^AGCTT
Ksp632I 1420 CTCTTC CTCTTC(1/4)
MaeI 1582 CTAG C^TAG
MaeII 946 ACGT A^CGT
MamI 556 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NspI 1751 ACATGC [GA]CATG^[CT]
PaeR7I 1645 CTCGAG C^TCGAG
PpuMI 1046 GGGACCT [GA]G^G[AT]CC[CT]
PvuI 543 CGATCG CGAT^CG
RcaI 50 TCATGA T^CATGA
SapI 1419 GCTCTTC GCTCTTC(1/4)
SbfI 198 CCTGCAGG CCTGCA^GG
SmaI 1775 CCCGGG CCC^GGG
SmlI 1645 CTCGAG C^T[CT][GA]AG
Sse8387I 198 CCTGCAGG CCTGCA^GG
SspI 485 AATATT AAT^ATT
XhoI 1645 CTCGAG C^TCGAG
XmaCI 1775 CCCGGG C^CCGGG
XmaI 1775 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 35 enzymes) #
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Acc65I 968 GGTACC G^GTACC
1126 GGTACC
AccII 1872 CGCG CG^CG
1874 CGCG
AcyI 1031 GGCGCC G[GA]^CG[CT]C
1585 GGCGCC
AhdI 448 GACCCCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
940 GACCTTACGTC
AlwNI 1117 CAGTATCTG CAG[ACGT][ACGT][ACGT]^CTG
1622 CAGTAGCTG
AocI 526 CCTGAGG CC^T[ACGT]AGG
568 CCTGAGG
Asp718I 968 GGTACC G^GTACC
1126 GGTACC
AspEI 448 GACCCCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
940 GACCTTACGTC
BalI 605 TGGCCA TGG^CCA
1595 TGGCCA
BbeI 1031 GGCGCC GGCGC^C
1585 GGCGCC
BsaHI 1031 GGCGCC G[GA]^CG[CT]C
1585 GGCGCC
BsaWI 691 TCCGGT [AT]^CCGG[AT]
1667 TCCGGT
BsmAI 409 GAGAC GTCTC(1/5)
1280 GAGAC
BsmI 28 GAATGC GAATGC(1/-1)
210 GAATGC
BsrDI 1172 CATTGC GCAATG(2/0)
4 GCAATG
BstAPI 1621 GCAGTAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1688 GCATCAGCTGC
BstUI 1872 CGCG CG^CG
1874 CGCG
Bsu36I 526 CCTGAGG CC^T[ACGT]AGG
568 CCTGAGG
Eam1105I 448 GACCCCTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
940 GACCTTACGTC
Eco81I 526 CCTGAGG CC^T[ACGT]AGG
568 CCTGAGG
EcoNI 87 CCTTCGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1035 CCTTCAGGAGG
EcoRI 193 GAATTC G^AATTC
427 GAATTC
Hin1I 1031 GGCGCC G[GA]^CG[CT]C
1585 GGCGCC
KasI 1031 GGCGCC G^GCGCC
1585 GGCGCC
KpnI 968 GGTACC GGTAC^C
1126 GGTACC
MluNI 605 TGGCCA TGG^CCA
1595 TGGCCA
MscI 605 TGGCCA TGG^CCA
1595 TGGCCA
MvnI 1872 CGCG CG^CG
1874 CGCG
NarI 1031 GGCGCC GG^CGCC
1585 GGCGCC
SfoI 1031 GGCGCC GGC^GCC
1585 GGCGCC
StuI 106 AGGCCT AGG^CCT
1262 AGGCCT
TaqI 854 TCGA T^CGA
1646 TCGA
Tsp45I 596 GTGAC ^GT[GC]AC
1318 GTGAC
TthHB8I 854 TCGA T^CGA
1646 TCGA
XcmI 309 CCAGAGAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
593 CCAGTGACTTTGTGG
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# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApaI ApoI AspHI AvaI AvaII
BanI BanII BbvCI BbvI BcnI
BglI BmyI BpmI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmFI
BsoBI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII EaeI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeIII
MboI MboII MflI MnlI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI TseI Tsp509I TspRI
XhoII