(Circular) (Six-base) MAPSORT of: pFN21AB6136.seq Check: 6026 from: 1 to: 6622 pFN21AB6136 6622 bp With 182 enzymes: SgfI * April 15, 2010 09:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2671 3337 2671 Size: 666 5956 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4432 278 Size: 53 83 186 936 2896 2468 Fragments arranged by size: 2896 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3965 1937 Size: 2028 4594 Acc65I G'GTAC_C Cuts at: 2973 3131 3943 2973 Size: 158 812 5652 Fragments arranged by size: 5652 812 158 AclI AA'CG_TT Cuts at: 1563 4749 5122 1563 Size: 3186 373 3063 Fragments arranged by size: 3186 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2199 2463 3026 3354 4685 6069 6337 1632 Size: 567 264 563 328 1331 1384 268 1917 Fragments arranged by size: 1917 1384 1331 567 563 328 268 264 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6425 AflIII A'CryG_T Cuts at: 1203 5521 6498 1203 Size: 4318 977 1327 Fragments arranged by size: 4318 1327 977 AgeI A'CCGG_T Cuts at: 5453 5453 Size: 6622 AhdI GACnn_n'nnGTC Cuts at: 2458 2950 5351 2458 Size: 492 2401 3729 Fragments arranged by size: 3729 2401 492 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6622 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6622 AlwNI CAG_nnn'CTG Cuts at: 3127 3632 4365 5937 3127 Size: 505 733 1572 3812 Fragments arranged by size: 3812 1572 733 505 ApaI G_GGCC'C Cuts at: 2362 3365 3649 3779 2362 Size: 1003 284 130 5205 Fragments arranged by size: 5205 1003 284 130 ApaLI G'TGCA_C Cuts at: 4679 5835 4679 Size: 1156 5466 ApoI r'AATT_y Cuts at: 1493 2198 2432 2654 3931 4157 4309 6418 1493 Size: 705 234 222 1277 226 152 2109 1697 Fragments arranged by size: 2109 1697 1277 705 234 226 222 152 AseI AT'TA_AT Cuts at: 160 1032 5176 160 Size: 872 4144 1606 Fragments arranged by size: 4144 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6622 AvaI C'yCGr_G Cuts at: 1870 1943 3650 3710 3780 3947 1870 Size: 73 1707 60 70 167 4545 Fragments arranged by size: 4545 1707 167 73 70 60 BamHI G'GATC_C Cuts at: 3097 3952 4319 3097 Size: 855 367 5400 Fragments arranged by size: 5400 855 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2042 2973 3036 3131 3590 Size: 325 217 627 255 931 63 95 459 Cuts at: 3590 3794 3943 5398 618 Size: 204 149 1455 1842 Fragments arranged by size: 1842 1455 931 627 459 325 255 217 204 149 95 63 BanII G_rGCy'C Cuts at: 1391 2137 2323 2362 2719 3365 3426 3649 3657 Size: 746 186 39 357 646 61 223 8 Cuts at: 3657 3718 3779 3941 1391 Size: 61 61 162 4072 Fragments arranged by size: 4072 746 646 357 223 186 162 61 61 61 39 8 BbeI G_GCGC'C Cuts at: 3040 3594 3040 Size: 554 6068 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5651 BbvCI CC'TCA_GC Cuts at: 2484 2913 3012 3548 2484 Size: 429 99 536 5558 Fragments arranged by size: 5558 536 429 99 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3691 3725 4813 4847 1903 Size: 34 1754 34 1088 34 3678 Fragments arranged by size: 3678 1754 1088 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3386 4514 5724 3386 Size: 1128 1210 4284 Fragments arranged by size: 4284 1210 1128 BclI T'GATC_A Cuts at: 1548 3004 1548 Size: 1456 5166 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2147 2480 3599 Size: 107 122 71 631 605 475 333 1119 Cuts at: 3599 3866 5233 136 Size: 267 1367 1525 Fragments arranged by size: 1525 1367 1119 631 605 475 333 267 122 107 71 BglII A'GATC_T Cuts at: 6617 6617 Size: 6622 BlpI GC'TnA_GC Cuts at: 4039 4039 Size: 6622 Bme1580I G_kGCm'C Cuts at: 1790 2073 2239 2362 3365 3649 3779 4683 5839 Size: 283 166 123 1003 284 130 904 1156 Cuts at: 5839 1790 Size: 2573 Fragments arranged by size: 2573 1156 1003 904 284 283 166 130 123 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6622 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2365 3368 4353 Size: 416 53 83 399 24 948 1003 985 Cuts at: 4353 5311 442 Size: 958 1753 Fragments arranged by size: 1753 1003 985 958 948 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6622 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2170 2170 Size: 6622 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2400 2711 3212 3641 3764 3835 5282 1239 Size: 1161 311 501 429 123 71 1447 2579 Fragments arranged by size: 2579 1447 1161 501 429 311 123 71 Bpu10I CC'TnA_GC Cuts at: 2484 2718 2913 3012 3102 3548 2484 Size: 234 195 99 90 446 5558 Fragments arranged by size: 5558 446 234 195 99 90 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4747 5612 5910 4747 Size: 865 298 5459 Fragments arranged by size: 5459 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5285 882 Size: 617 3786 2219 Fragments arranged by size: 3786 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4413 493 Size: 711 3209 2702 Fragments arranged by size: 3209 2702 711 BsaBI GATnn'nnATC Cuts at: 2565 6616 2565 Size: 4051 2571 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3037 3591 4429 4811 Size: 53 83 186 936 1504 554 838 382 Cuts at: 4811 5446 275 Size: 635 1451 Fragments arranged by size: 1504 1451 936 838 635 554 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2696 3672 5055 5453 5727 5874 6285 2696 Size: 976 1383 398 274 147 411 3033 Fragments arranged by size: 3033 1383 976 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2373 2403 2501 2531 3440 Size: 30 165 30 970 30 98 30 909 Cuts at: 3440 3470 1178 Size: 30 4330 Fragments arranged by size: 4330 970 909 165 98 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2522 2591 2906 2999 3002 1186 Size: 1336 69 315 93 3 4806 Fragments arranged by size: 4806 1336 315 93 69 3 BseYI C'CCAG_C Cuts at: 2478 2521 3365 5825 6321 2478 Size: 43 844 2460 496 2779 Fragments arranged by size: 2779 2460 844 496 43 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2721 3110 3494 1163 Size: 573 985 389 384 4291 Fragments arranged by size: 4291 985 573 389 384 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2551 4072 4833 5861 1540 Size: 275 186 550 1521 761 1028 2301 Fragments arranged by size: 2301 1521 1028 761 550 275 186 BsiHKAI G_wGCw'C Cuts at: 2719 3426 3657 3738 3821 4683 4768 5839 2719 Size: 707 231 81 83 862 85 1071 3502 Fragments arranged by size: 3502 1071 862 707 231 85 83 81 BsmI GAATG_Cn' Cuts at: 2039 2221 4133 4226 2039 Size: 182 1912 93 4435 Fragments arranged by size: 4435 1912 182 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2073 2137 2239 2323 2362 2719 3365 Size: 399 283 64 102 84 39 357 646 Cuts at: 3365 3426 3649 3657 3718 3738 3779 3821 3941 Size: 61 223 8 61 20 41 42 120 Cuts at: 3941 4683 4768 5839 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 646 399 357 283 223 120 102 85 84 64 61 61 42 41 39 20 8 BspEI T'CCGG_A Cuts at: 6285 6285 Size: 6622 BspHI T'CATG_A Cuts at: 2055 4511 6241 2055 Size: 2456 1730 2436 Fragments arranged by size: 2456 2436 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2671 3063 3337 3977 844 Size: 1827 392 274 640 3489 Fragments arranged by size: 3489 1827 640 392 274 BsrBI CCG'CTC Cuts at: 1394 1823 2053 4509 1394 Size: 429 230 2456 3507 Fragments arranged by size: 3507 2456 429 230 BsrDI GCAATG_nn' Cuts at: 65 1248 2016 3176 5117 5291 65 Size: 1183 768 1160 1941 174 1396 Fragments arranged by size: 1941 1396 1183 1160 768 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5266 5453 1700 Size: 110 3456 187 2869 Fragments arranged by size: 3456 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6622 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6622 BssSI C'ACGA_G Cuts at: 4682 5694 4682 Size: 1012 5610 BstAPI GCAn_nnn'nTGC Cuts at: 794 3632 3699 794 Size: 2838 67 3717 Fragments arranged by size: 3717 2838 67 BstBI TT'CG_AA Cuts at: 5426 5426 Size: 6622 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6622 BstXI CCAn_nnnn'nTGG Cuts at: 2164 2164 Size: 6622 BstYI r'GATC_y Cuts at: 1972 2581 3097 3383 3883 3952 4319 4704 4721 Size: 609 516 286 500 69 367 385 17 Cuts at: 4721 6173 6259 6271 6617 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 609 516 500 385 367 346 286 86 69 17 12 Bsu36I CC'TnA_GG Cuts at: 2532 2574 2532 Size: 42 6580 BtgI C'CryG_G Cuts at: 513 1065 2004 3525 513 Size: 552 939 1521 3610 Fragments arranged by size: 3610 1521 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3870 487 Size: 683 56 2644 3239 Fragments arranged by size: 3239 2644 683 56 BtsI GCAGTG_nn' Cuts at: 3482 4138 4932 4952 3482 Size: 656 794 20 5152 Fragments arranged by size: 5152 794 656 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6622 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3487 3522 529 Size: 35 2923 35 3629 Fragments arranged by size: 3629 2923 35 35 DraI TTT'AAA Cuts at: 3926 3926 Size: 6622 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2422 5629 809 Size: 507 1106 3207 1802 Fragments arranged by size: 3207 1802 1106 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2595 2610 3600 Size: 54 1425 50 275 18 765 15 990 Cuts at: 3600 4069 4958 6587 8 Size: 469 889 1629 43 Fragments arranged by size: 1629 1425 990 889 765 469 275 54 50 43 18 15 EagI C'GGCC_G Cuts at: 1537 1812 4069 1537 Size: 275 2257 4090 Fragments arranged by size: 4090 2257 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3431 4552 1353 Size: 2078 1121 3423 Fragments arranged by size: 3423 2078 1121 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2291 5209 5583 5729 387 Size: 1064 138 702 2918 374 146 1280 Fragments arranged by size: 2918 1280 1064 702 374 146 138 EcoICRI GAG'CTC Cuts at: 2717 3424 3655 2717 Size: 707 231 5684 Fragments arranged by size: 5684 707 231 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2199 2400 2463 2711 3026 3212 3354 Size: 393 567 201 63 248 315 186 142 Cuts at: 3354 3641 3764 3835 4685 5282 6069 6337 1239 Size: 287 123 71 850 597 787 268 1524 Fragments arranged by size: 1524 850 787 597 567 393 315 287 268 248 201 186 142 123 71 63 EcoNI CCTnn'n_nnAGG Cuts at: 2096 3044 3917 2096 Size: 948 873 4801 Fragments arranged by size: 4801 948 873 EcoO109I rG'GnC_Cy Cuts at: 2089 3052 3362 3646 3748 3775 3776 2089 Size: 963 310 284 102 27 1 4935 Fragments arranged by size: 4935 963 310 284 102 27 1 EcoRI G'AATT_C Cuts at: 2198 2432 3931 4309 2198 Size: 234 1499 378 4511 Fragments arranged by size: 4511 1499 378 234 FspI TGC'GCA Cuts at: 5128 5128 Size: 6622 HaeII r_GCGC'y Cuts at: 1678 3040 3230 3594 5769 1678 Size: 1362 190 364 2175 2531 Fragments arranged by size: 2531 2175 1362 364 190 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2444 2476 2936 2968 3440 Size: 32 135 32 872 32 460 32 472 Cuts at: 3440 3472 5263 5295 5337 5369 1373 Size: 32 1791 32 42 32 2626 Fragments arranged by size: 2626 1791 872 472 460 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2779 3966 4212 669 Size: 988 281 841 1187 246 3079 Fragments arranged by size: 3079 1187 988 841 281 246 HindIII A'AGCT_T Cuts at: 748 3114 748 Size: 2366 4256 HpaI GTT'AAC Cuts at: 4212 4212 Size: 6622 KasI G'GCGC_C Cuts at: 3036 3590 3036 Size: 554 6068 KpnI G_GTAC'C Cuts at: 2977 3135 3947 2977 Size: 158 812 5652 Fragments arranged by size: 5652 812 158 MfeI C'AATT_G Cuts at: 4221 4221 Size: 6622 MluI A'CGCG_T Cuts at: 6498 6498 Size: 6622 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2868 3783 5736 5920 1305 Size: 1563 915 1953 184 2007 Fragments arranged by size: 2007 1953 1563 915 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2612 3602 10 Size: 54 1425 343 780 990 3030 Fragments arranged by size: 3030 1425 990 780 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 4580 4939 5098 518 Size: 648 843 2571 359 159 2042 Fragments arranged by size: 2571 2042 843 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2378 2478 3086 3437 3542 3699 4038 4715 Size: 772 100 608 351 105 157 339 677 Cuts at: 4715 5863 6108 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 772 677 608 351 339 245 157 105 100 NarI GG'CG_CC Cuts at: 3037 3591 3037 Size: 554 6068 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5131 Fragments arranged by size: 5131 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6622 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6622 NotI GC'GGCC_GC Cuts at: 4069 4069 Size: 6622 NspI r_CATG'y Cuts at: 1132 1229 3760 3980 5525 1132 Size: 97 2531 220 1545 2229 Fragments arranged by size: 2531 2229 1545 220 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6622 PciI A'CATG_T Cuts at: 5521 5521 Size: 6622 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6622 PmeI GTTT'AAAC Cuts at: 3926 3926 Size: 6622 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4691 4723 6204 6236 4691 Size: 32 1481 32 5077 Fragments arranged by size: 5077 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3052 3052 Size: 6622 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6622 PsiI TTA'TAA Cuts at: 2409 4192 2409 Size: 1783 4839 PspOMI G'GGCC_C Cuts at: 2358 3361 3645 3775 2358 Size: 1003 284 130 5205 Fragments arranged by size: 5205 1003 284 130 PspXI vC'TCGA_Gb Cuts at: 3650 3650 Size: 6622 PstI C_TGCA'G Cuts at: 830 2208 2589 3261 3974 830 Size: 1378 381 672 713 3478 Fragments arranged by size: 3478 1378 713 672 381 PvuI CG_AT'CG Cuts at: 2001 2551 2001 Size: 550 6072 PvuII CAG'CTG Cuts at: 2378 3086 3437 3542 3699 2378 Size: 708 351 105 157 5301 Fragments arranged by size: 5301 708 351 157 105 SacI G_AGCT'C Cuts at: 2719 3426 3657 2719 Size: 707 231 5684 Fragments arranged by size: 5684 707 231 SalI G'TCGA_C Cuts at: 1936 3964 1936 Size: 2028 4594 SapI GCTCTTCn'nnn_ Cuts at: 3431 3431 Size: 6622 SbfI CC_TGCA'GG Cuts at: 2208 3974 2208 Size: 1766 4856 ScaI AGT'ACT Cuts at: 4870 6582 4870 Size: 1712 4910 SfcI C'TryA_G Cuts at: 826 1045 2204 2585 2844 3257 3970 5105 5786 Size: 219 1159 381 259 413 713 1135 681 Cuts at: 5786 5977 826 Size: 191 1471 Fragments arranged by size: 1471 1159 1135 713 681 413 381 259 219 191 SfoI GGC'GCC Cuts at: 3038 3592 3038 Size: 554 6068 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6622 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6622 SmaI CCC'GGG Cuts at: 1872 3782 3949 1872 Size: 1910 167 4545 Fragments arranged by size: 4545 1910 167 SmlI C'TyrA_G Cuts at: 820 1017 1943 3650 4726 5627 5889 820 Size: 197 926 1707 1076 901 262 1553 Fragments arranged by size: 1707 1553 1076 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4413 493 Size: 3920 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6622 SphI G_CATG'C Cuts at: 1132 3980 1132 Size: 2848 3774 SspI AAT'ATT Cuts at: 5 52 2492 4546 5 Size: 47 2440 2054 2081 Fragments arranged by size: 2440 2081 2054 47 StuI AGG'CCT Cuts at: 1517 2113 3269 1517 Size: 596 1156 4870 Fragments arranged by size: 4870 1156 596 StyI C'CwwG_G Cuts at: 513 1065 2004 4061 513 Size: 552 939 2057 3074 Fragments arranged by size: 3074 2057 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2093 3152 4649 4666 4819 5004 5449 Size: 1255 513 1059 1497 17 153 185 445 Cuts at: 5449 325 Size: 1498 Fragments arranged by size: 1498 1497 1255 1059 513 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4868 6580 96 Size: 274 80 33 51 1443 2891 1712 138 Fragments arranged by size: 2891 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2373 2405 2501 2533 6582 6614 2373 Size: 32 96 32 4049 32 2381 Fragments arranged by size: 4049 2381 96 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6622 XbaI T'CTAG_A Cuts at: 3958 3958 Size: 6622 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2321 2605 2321 Size: 284 6338 XhoI C'TCGA_G Cuts at: 1943 3650 1943 Size: 1707 4915 XmaI C'CCGG_G Cuts at: 1870 3780 3947 1870 Size: 1910 167 4545 Fragments arranged by size: 4545 1910 167 XmnI GAAnn'nnTTC Cuts at: 4751 4751 Size: 6622 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4430 276 Size: 53 83 186 936 2896 2468 Fragments arranged by size: 2896 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I DraIII EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PsrI RsrII SacII SanDI SexAI SfiI SrfI SwaI