Restriction map for
pFN21AB6136
(Linear) (Six-base) MAPSORT of: KIBB6136.seq Check: 8327 from: 1 to: 1917
KIBB6136.orf
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 666 1332 1917
Size: 666 666 585
Fragments arranged by size:
666 666 585
Acc65I G'GTAC_C
Cuts at: 0 968 1126 1917
Size: 968 158 791
Fragments arranged by size:
968 791 158
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 194 458 1021 1349 1917
Size: 194 264 563 328 568
Fragments arranged by size:
568 563 328 264 194
AhdI GACnn_n'nnGTC
Cuts at: 0 453 945 1917
Size: 453 492 972
Fragments arranged by size:
972 492 453
AlwNI CAG_nnn'CTG
Cuts at: 0 1122 1627 1917
Size: 1122 505 290
Fragments arranged by size:
1122 505 290
ApaI G_GGCC'C
Cuts at: 0 357 1360 1644 1774 1917
Size: 357 1003 284 130 143
Fragments arranged by size:
1003 357 284 143 130
ApoI r'AATT_y
Cuts at: 0 193 427 649 1917
Size: 193 234 222 1268
Fragments arranged by size:
1268 234 222 193
AvaI C'yCGr_G
Cuts at: 0 1645 1705 1775 1917
Size: 1645 60 70 142
Fragments arranged by size:
1645 142 70 60
BamHI G'GATC_C
Cuts at: 0 1092 1917
Size: 1092 825
BanI G'GyrC_C
Cuts at: 0 37 968 1031 1126 1585 1789 1917
Size: 37 931 63 95 459 204 128
Fragments arranged by size:
931 459 204 128 95 63 37
BanII G_rGCy'C
Cuts at: 0 132 318 357 714 1360 1421 1644 1652
Size: 132 186 39 357 646 61 223 8
Cuts at: 1652 1713 1774 1917
Size: 61 61 143
Fragments arranged by size:
646 357 223 186 143 132 61 61
61 39 8
BbeI G_GCGC'C
Cuts at: 0 1035 1589 1917
Size: 1035 554 328
Fragments arranged by size:
1035 554 328
BbvCI CC'TCA_GC
Cuts at: 0 479 908 1007 1543 1917
Size: 479 429 99 536 374
Fragments arranged by size:
536 479 429 374 99
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1686 1720 1917
Size: 1686 34 197
Fragments arranged by size:
1686 197 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1381 1917
Size: 1381 536
BclI T'GATC_A
Cuts at: 0 999 1917
Size: 999 918
BglI GCCn_nnn'nGGC
Cuts at: 0 142 475 1594 1861 1917
Size: 142 333 1119 267 56
Fragments arranged by size:
1119 333 267 142 56
Bme1580I G_kGCm'C
Cuts at: 0 68 234 357 1360 1644 1774 1917
Size: 68 166 123 1003 284 130 143
Fragments arranged by size:
1003 284 166 143 130 123 68
BmrI ACTGGGnnnn_n'
Cuts at: 0 360 1363 1917
Size: 360 1003 554
Fragments arranged by size:
1003 554 360
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 165 1917
Size: 165 1752
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 395 706 1207 1636 1759 1830 1917
Size: 395 311 501 429 123 71 87
Fragments arranged by size:
501 429 395 311 123 87 71
Bpu10I CC'TnA_GC
Cuts at: 0 479 713 908 1007 1097 1543 1917
Size: 479 234 195 99 90 446 374
Fragments arranged by size:
479 446 374 234 195 99 90
BsaBI GATnn'nnATC
Cuts at: 0 560 1917
Size: 560 1357
BsaHI Gr'CG_yC
Cuts at: 0 1032 1586 1917
Size: 1032 554 331
Fragments arranged by size:
1032 554 331
BsaWI w'CCGG_w
Cuts at: 0 691 1667 1917
Size: 691 976 250
Fragments arranged by size:
976 691 250
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 368 398 496 526 1435 1465 1917
Size: 368 30 98 30 909 30 452
Fragments arranged by size:
909 452 368 98 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 517 586 901 994 997 1917
Size: 517 69 315 93 3 920
Fragments arranged by size:
920 517 315 93 69 3
BseYI C'CCAG_C
Cuts at: 0 473 516 1360 1917
Size: 473 43 844 557
Fragments arranged by size:
844 557 473 43
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 716 1105 1489 1917
Size: 716 389 384 428
Fragments arranged by size:
716 428 389 384
BsiEI CG_ry'CG
Cuts at: 0 546 1917
Size: 546 1371
BsiHKAI G_wGCw'C
Cuts at: 0 714 1421 1652 1733 1816 1917
Size: 714 707 231 81 83 101
Fragments arranged by size:
714 707 231 101 83 81
BsmI GAATG_Cn'
Cuts at: 0 34 216 1917
Size: 34 182 1701
Fragments arranged by size:
1701 182 34
Bsp1286I G_dGCh'C
Cuts at: 0 68 132 234 318 357 714 1360 1421
Size: 68 64 102 84 39 357 646 61
Cuts at: 1421 1644 1652 1713 1733 1774 1816 1917
Size: 223 8 61 20 41 42 101
Fragments arranged by size:
646 357 223 102 101 84 68 64
61 61 42 41 39 20 8
BspHI T'CATG_A
Cuts at: 0 50 1917
Size: 50 1867
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 666 1058 1332 1917
Size: 666 392 274 585
Fragments arranged by size:
666 585 392 274
BsrBI CCG'CTC
Cuts at: 0 48 1917
Size: 48 1869
BsrDI GCAATG_nn'
Cuts at: 0 11 1171 1917
Size: 11 1160 746
Fragments arranged by size:
1160 746 11
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1627 1694 1917
Size: 1627 67 223
Fragments arranged by size:
1627 223 67
BstXI CCAn_nnnn'nTGG
Cuts at: 0 159 1917
Size: 159 1758
BstYI r'GATC_y
Cuts at: 0 576 1092 1378 1878 1917
Size: 576 516 286 500 39
Fragments arranged by size:
576 516 500 286 39
Bsu36I CC'TnA_GG
Cuts at: 0 527 569 1917
Size: 527 42 1348
Fragments arranged by size:
1348 527 42
BtgI C'CryG_G
Cuts at: 0 1520 1917
Size: 1520 397
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1865 1917
Size: 1865 52
BtsI GCAGTG_nn'
Cuts at: 0 1477 1917
Size: 1477 440
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1482 1517 1917
Size: 1482 35 400
Fragments arranged by size:
1482 400 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 417 1917
Size: 417 1500
EaeI y'GGCC_r
Cuts at: 0 590 605 1595 1917
Size: 590 15 990 322
Fragments arranged by size:
990 590 322 15
EarI CTCTTCn'nnn_
Cuts at: 0 1426 1917
Size: 1426 491
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 286 1917
Size: 286 1631
EcoICRI GAG'CTC
Cuts at: 0 712 1419 1650 1917
Size: 712 707 231 267
Fragments arranged by size:
712 707 267 231
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 194 395 458 706 1021 1207 1349 1636
Size: 194 201 63 248 315 186 142 287
Cuts at: 1636 1759 1830 1917
Size: 123 71 87
Fragments arranged by size:
315 287 248 201 194 186 142 123
87 71 63
EcoNI CCTnn'n_nnAGG
Cuts at: 0 91 1039 1917
Size: 91 948 878
Fragments arranged by size:
948 878 91
EcoO109I rG'GnC_Cy
Cuts at: 0 84 1047 1357 1641 1743 1770 1771 1917
Size: 84 963 310 284 102 27 1 146
Fragments arranged by size:
963 310 284 146 102 84 27 1
EcoRI G'AATT_C
Cuts at: 0 193 427 1917
Size: 193 234 1490
Fragments arranged by size:
1490 234 193
HaeII r_GCGC'y
Cuts at: 0 1035 1225 1589 1917
Size: 1035 190 364 328
Fragments arranged by size:
1035 364 328 190
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 439 471 931 963 1435 1467 1917
Size: 439 32 460 32 472 32 450
Fragments arranged by size:
472 460 450 439 32 32 32
HincII GTy'rAC
Cuts at: 0 774 1917
Size: 774 1143
HindIII A'AGCT_T
Cuts at: 0 1109 1917
Size: 1109 808
KasI G'GCGC_C
Cuts at: 0 1031 1585 1917
Size: 1031 554 332
Fragments arranged by size:
1031 554 332
KpnI G_GTAC'C
Cuts at: 0 972 1130 1917
Size: 972 158 787
Fragments arranged by size:
972 787 158
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 863 1778 1917
Size: 863 915 139
Fragments arranged by size:
915 863 139
MscI TGG'CCA
Cuts at: 0 607 1597 1917
Size: 607 990 320
Fragments arranged by size:
990 607 320
MspA1I CmG'CkG
Cuts at: 0 373 473 1081 1432 1537 1694 1917
Size: 373 100 608 351 105 157 223
Fragments arranged by size:
608 373 351 223 157 105 100
NarI GG'CG_CC
Cuts at: 0 1032 1586 1917
Size: 1032 554 331
Fragments arranged by size:
1032 554 331
NspI r_CATG'y
Cuts at: 0 1755 1917
Size: 1755 162
PpuMI rG'GwC_Cy
Cuts at: 0 1047 1917
Size: 1047 870
PsiI TTA'TAA
Cuts at: 0 404 1917
Size: 404 1513
PspOMI G'GGCC_C
Cuts at: 0 353 1356 1640 1770 1917
Size: 353 1003 284 130 147
Fragments arranged by size:
1003 353 284 147 130
PspXI vC'TCGA_Gb
Cuts at: 0 1645 1917
Size: 1645 272
PstI C_TGCA'G
Cuts at: 0 203 584 1256 1917
Size: 203 381 672 661
Fragments arranged by size:
672 661 381 203
PvuI CG_AT'CG
Cuts at: 0 546 1917
Size: 546 1371
PvuII CAG'CTG
Cuts at: 0 373 1081 1432 1537 1694 1917
Size: 373 708 351 105 157 223
Fragments arranged by size:
708 373 351 223 157 105
SacI G_AGCT'C
Cuts at: 0 714 1421 1652 1917
Size: 714 707 231 265
Fragments arranged by size:
714 707 265 231
SapI GCTCTTCn'nnn_
Cuts at: 0 1426 1917
Size: 1426 491
SbfI CC_TGCA'GG
Cuts at: 0 203 1917
Size: 203 1714
SfcI C'TryA_G
Cuts at: 0 199 580 839 1252 1917
Size: 199 381 259 413 665
Fragments arranged by size:
665 413 381 259 199
SfoI GGC'GCC
Cuts at: 0 1033 1587 1917
Size: 1033 554 330
Fragments arranged by size:
1033 554 330
SmaI CCC'GGG
Cuts at: 0 1777 1917
Size: 1777 140
SmlI C'TyrA_G
Cuts at: 0 1645 1917
Size: 1645 272
SspI AAT'ATT
Cuts at: 0 487 1917
Size: 487 1430
StuI AGG'CCT
Cuts at: 0 108 1264 1917
Size: 108 1156 653
Fragments arranged by size:
1156 653 108
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 88 1147 1917
Size: 88 1059 770
Fragments arranged by size:
1059 770 88
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 368 400 496 528 1917
Size: 368 32 96 32 1389
Fragments arranged by size:
1389 368 96 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 316 600 1917
Size: 316 284 1317
Fragments arranged by size:
1317 316 284
XhoI C'TCGA_G
Cuts at: 0 1645 1917
Size: 1645 272
XmaI C'CCGG_G
Cuts at: 0 1775 1917
Size: 1775 142
Enzymes that do cut:
AarI Acc65I AcuI AhdI AlwNI ApaI ApoI AvaI
BamHI BanI BanII BbeI BbvCI BcgI BciVI BclI
BglI Bme1580I BmrI BplI BpmI Bpu10I BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspHI BspMI BsrBI BsrDI BstAPI BstXI BstYI
Bsu36I BtgI BtgZI BtsI CspCI DrdI EaeI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI HaeII Hin4I
HincII HindIII KasI KpnI MmeI MscI MspA1I NarI
NspI PpuMI PsiI PspOMI PspXI PstI PvuI PvuII
SacI SapI SbfI SfcI SfoI SmaI SmlI SspI
StuI TaqII TstI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AflIII AgeI AleI
AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI
BbsI BfrBI BglII BlpI BmgBI BmtI BpuEI BsaI
BsaAI BsiWI BsmBI BspEI BsrFI BsrGI BssHII BssSI
BstBI BstEII BstZ17I ClaI DraI DraIII EagI EcoRV
FalI FseI FspI FspAI HpaI MfeI MluI MslI
NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PflMI PfoI PmeI PmlI PpiI
PshAI PsrI RsrII SacII SalI SanDI ScaI SexAI
SfiI SgfI SgrAI SnaBI SpeI SphI SrfI StyI
SwaI TatI Tth111I XbaI XmnI ZraI