(Circular) (Six-base) MAPSORT of: pFN21AB6124.seq Check: 7606 from: 1 to: 6583 pFN21AB6124 6583 bp With 182 enzymes: SgfI * April 15, 2010 09:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4393 278 Size: 53 83 186 936 2857 2468 Fragments arranged by size: 2857 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3063 3926 1937 Size: 1126 863 4594 Fragments arranged by size: 4594 1126 863 Acc65I G'GTAC_C Cuts at: 2090 2299 3904 2090 Size: 209 1605 4769 Fragments arranged by size: 4769 1605 209 AclI AA'CG_TT Cuts at: 1563 4710 5083 1563 Size: 3147 373 3063 Fragments arranged by size: 3147 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2757 4646 6030 6298 1632 Size: 1125 1889 1384 268 1917 Fragments arranged by size: 1917 1889 1384 1125 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6386 AflIII A'CryG_T Cuts at: 1203 5482 6459 1203 Size: 4279 977 1327 Fragments arranged by size: 4279 1327 977 AgeI A'CCGG_T Cuts at: 5414 5414 Size: 6583 AhdI GACnn_n'nnGTC Cuts at: 5312 5312 Size: 6583 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6583 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6583 AlwNI CAG_nnn'CTG Cuts at: 2528 3836 4326 5898 2528 Size: 1308 490 1572 3213 Fragments arranged by size: 3213 1572 1308 490 ApaI G_GGCC'C Cuts at: 2063 2063 Size: 6583 ApaLI G'TGCA_C Cuts at: 4640 5796 4640 Size: 1156 5427 ApoI r'AATT_y Cuts at: 1493 3218 3892 4118 4270 6379 1493 Size: 1725 674 226 152 2109 1697 Fragments arranged by size: 2109 1725 1697 674 226 152 AseI AT'TA_AT Cuts at: 160 1032 5137 160 Size: 872 4105 1606 Fragments arranged by size: 4105 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6583 AvaI C'yCGr_G Cuts at: 1870 1943 2056 2649 3468 3908 1870 Size: 73 113 593 819 440 4545 Fragments arranged by size: 4545 819 593 440 113 73 BamHI G'GATC_C Cuts at: 3913 4280 3913 Size: 367 6216 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2090 2299 2384 3904 5359 Size: 325 217 627 303 209 85 1520 1455 Cuts at: 5359 618 Size: 1842 Fragments arranged by size: 1842 1520 1455 627 325 303 217 209 85 BanII G_rGCy'C Cuts at: 1391 2063 3902 1391 Size: 672 1839 4072 Fragments arranged by size: 4072 1839 672 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2524 2793 2910 928 Size: 971 625 269 117 4601 Fragments arranged by size: 4601 971 625 269 117 BbvCI CC'TCA_GC Cuts at: 2936 3504 2936 Size: 568 6015 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4774 4808 1903 Size: 34 2837 34 3678 Fragments arranged by size: 3678 2837 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4475 5685 4475 Size: 1210 5373 BclI T'GATC_A Cuts at: 1548 1548 Size: 6583 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5194 136 Size: 107 122 71 631 605 3522 1525 Fragments arranged by size: 3522 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6578 6578 Size: 6583 BlpI GC'TnA_GC Cuts at: 4000 4000 Size: 6583 Bme1580I G_kGCm'C Cuts at: 1790 2063 4644 5800 1790 Size: 273 2581 1156 2573 Fragments arranged by size: 2581 2573 1156 273 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6583 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3777 3851 4314 Size: 416 53 83 399 24 2360 74 463 Cuts at: 4314 5272 442 Size: 958 1753 Fragments arranged by size: 2360 1753 958 463 416 399 83 74 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6583 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2644 3603 2644 Size: 959 5624 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2748 3432 3544 3591 3624 5243 1239 Size: 1509 684 112 47 33 1619 2579 Fragments arranged by size: 2579 1619 1509 684 112 47 33 Bpu10I CC'TnA_GC Cuts at: 2348 2936 3504 3678 2348 Size: 588 568 174 5253 Fragments arranged by size: 5253 588 568 174 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4708 5573 5871 4708 Size: 865 298 5420 Fragments arranged by size: 5420 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5246 882 Size: 617 3747 2219 Fragments arranged by size: 3747 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4374 493 Size: 711 3170 2702 Fragments arranged by size: 3170 2702 711 BsaBI GATnn'nnATC Cuts at: 6577 6577 Size: 6583 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2254 4390 4772 5407 Size: 53 83 186 936 721 2136 382 635 Cuts at: 5407 275 Size: 1451 Fragments arranged by size: 2136 1451 936 721 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5016 5414 5688 5835 6246 5016 Size: 398 274 147 411 5353 Fragments arranged by size: 5353 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6358 Fragments arranged by size: 6358 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2620 2623 1186 Size: 1434 3 5146 Fragments arranged by size: 5146 1434 3 BseYI C'CCAG_C Cuts at: 3473 5786 6282 3473 Size: 2313 496 3774 Fragments arranged by size: 3774 2313 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2827 1163 Size: 573 1091 4919 Fragments arranged by size: 4919 1091 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4033 4794 5822 1540 Size: 275 186 2032 761 1028 2301 Fragments arranged by size: 2301 2032 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2734 4644 4729 5800 2734 Size: 1910 85 1071 3517 Fragments arranged by size: 3517 1910 1071 85 BsmI GAATG_Cn' Cuts at: 2965 4094 4187 2965 Size: 1129 93 5361 Fragments arranged by size: 5361 1129 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2063 2734 3902 4644 4729 5800 1391 Size: 399 273 671 1168 742 85 1071 2174 Fragments arranged by size: 2174 1168 1071 742 671 399 273 85 BspEI T'CCGG_A Cuts at: 6246 6246 Size: 6583 BspHI T'CATG_A Cuts at: 3273 3813 4472 6202 3273 Size: 540 659 1730 3654 Fragments arranged by size: 3654 1730 659 540 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2079 2598 2816 3938 844 Size: 1235 519 218 1122 3489 Fragments arranged by size: 3489 1235 1122 519 218 BsrBI CCG'CTC Cuts at: 1394 1823 2656 4470 1394 Size: 429 833 1814 3507 Fragments arranged by size: 3507 1814 833 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2322 3807 5078 5252 65 Size: 1183 1074 1485 1271 174 1396 Fragments arranged by size: 1485 1396 1271 1183 1074 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5227 5414 1700 Size: 110 3417 187 2869 Fragments arranged by size: 3417 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6583 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6583 BssSI C'ACGA_G Cuts at: 3577 4643 5655 3577 Size: 1066 1012 4505 Fragments arranged by size: 4505 1066 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6583 BstBI TT'CG_AA Cuts at: 5387 5387 Size: 6583 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6583 BstXI CCAn_nnnn'nTGG Cuts at: 2101 2890 3340 2101 Size: 789 450 5344 Fragments arranged by size: 5344 789 450 BstYI r'GATC_y Cuts at: 1972 2538 3913 4280 4665 4682 6134 6220 6232 Size: 566 1375 367 385 17 1452 86 12 Cuts at: 6232 6578 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1375 566 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3872 513 Size: 552 939 1868 3224 Fragments arranged by size: 3224 1868 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3525 487 Size: 683 56 2299 3545 Fragments arranged by size: 3545 2299 683 56 BtsI GCAGTG_nn' Cuts at: 4099 4893 4913 4099 Size: 794 20 5769 Fragments arranged by size: 5769 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6583 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6548 DraI TTT'AAA Cuts at: 3223 3627 3887 3223 Size: 404 260 5919 Fragments arranged by size: 5919 404 260 DraIII CAC_nnn'GTG Cuts at: 3120 3120 Size: 6583 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5590 809 Size: 507 4274 1802 Fragments arranged by size: 4274 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2876 3875 4030 Size: 54 1425 50 275 18 1046 999 155 Cuts at: 4030 4919 6548 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1046 999 889 275 155 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4030 1537 Size: 275 2218 4090 Fragments arranged by size: 4090 2218 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3607 4513 1353 Size: 2254 906 3423 Fragments arranged by size: 3423 2254 906 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5170 5544 5690 387 Size: 1064 138 3581 374 146 1280 Fragments arranged by size: 3581 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2748 2757 3432 3544 3591 3624 4646 Size: 393 1116 9 675 112 47 33 1022 Cuts at: 4646 5243 6030 6298 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1116 1022 787 675 597 393 268 112 47 33 9 EcoO109I rG'GnC_Cy Cuts at: 2060 3352 3432 2060 Size: 1292 80 5211 Fragments arranged by size: 5211 1292 80 EcoRI G'AATT_C Cuts at: 3892 4270 3892 Size: 378 6205 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2530 2530 Size: 6583 FspI TGC'GCA Cuts at: 5089 5089 Size: 6583 HaeII r_GCGC'y Cuts at: 1678 5730 1678 Size: 4052 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2036 2068 2786 2818 3755 Size: 32 135 32 464 32 718 32 937 Cuts at: 3755 3787 5224 5256 5298 5330 1373 Size: 32 1437 32 42 32 2626 Fragments arranged by size: 2626 1437 937 718 464 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3856 3927 4173 669 Size: 988 281 1918 71 246 3079 Fragments arranged by size: 3079 1918 988 281 246 71 HindIII A'AGCT_T Cuts at: 748 2836 748 Size: 2088 4495 HpaI GTT'AAC Cuts at: 4173 4173 Size: 6583 KpnI G_GTAC'C Cuts at: 2094 2303 3908 2094 Size: 209 1605 4769 Fragments arranged by size: 4769 1605 209 MfeI C'AATT_G Cuts at: 2952 4182 2952 Size: 1230 5353 MluI A'CGCG_T Cuts at: 6459 6459 Size: 6583 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5697 5881 1305 Size: 4392 184 2007 Fragments arranged by size: 4392 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2878 10 Size: 54 1425 343 1046 3715 Fragments arranged by size: 3715 1425 1046 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2888 2970 3141 4541 4900 5059 518 Size: 648 1722 82 171 1400 359 159 2042 Fragments arranged by size: 2042 1722 1400 648 359 171 159 82 MspA1I CmG'CkG Cuts at: 1606 2940 3880 3999 4676 5824 6069 1606 Size: 1334 940 119 677 1148 245 2120 Fragments arranged by size: 2120 1334 1148 940 677 245 119 NcoI C'CATG_G Cuts at: 513 1065 2004 3872 513 Size: 552 939 1868 3224 Fragments arranged by size: 3224 1868 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6583 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6583 NotI GC'GGCC_GC Cuts at: 4030 4030 Size: 6583 NspI r_CATG'y Cuts at: 1132 1229 2310 3403 3941 5486 1132 Size: 97 1081 1093 538 1545 2229 Fragments arranged by size: 2229 1545 1093 1081 538 97 PasI CC'CwG_GG Cuts at: 1348 2705 1348 Size: 1357 5226 PciI A'CATG_T Cuts at: 5482 5482 Size: 6583 PflMI CCAn_nnn'nTGG Cuts at: 1108 2020 3391 1108 Size: 912 1371 4300 Fragments arranged by size: 4300 1371 912 PfoI T'CCnGG_A Cuts at: 2321 3339 3607 2321 Size: 1018 268 5297 Fragments arranged by size: 5297 1018 268 PmeI GTTT'AAAC Cuts at: 3887 3887 Size: 6583 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4652 4684 6165 6197 4652 Size: 32 1481 32 5038 Fragments arranged by size: 5038 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3352 3432 3352 Size: 80 6503 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6583 PsiI TTA'TAA Cuts at: 4153 4153 Size: 6583 PspOMI G'GGCC_C Cuts at: 2059 2059 Size: 6583 PstI C_TGCA'G Cuts at: 830 3935 830 Size: 3105 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6583 PvuII CAG'CTG Cuts at: 2940 2940 Size: 6583 SalI G'TCGA_C Cuts at: 1936 3925 1936 Size: 1989 4594 SanDI GG'GwC_CC Cuts at: 3352 3352 Size: 6583 SbfI CC_TGCA'GG Cuts at: 3935 3935 Size: 6583 ScaI AGT'ACT Cuts at: 4831 6543 4831 Size: 1712 4871 SfcI C'TryA_G Cuts at: 826 1045 2548 2749 3931 5066 5747 5938 826 Size: 219 1503 201 1182 1135 681 191 1471 Fragments arranged by size: 1503 1471 1182 1135 681 219 201 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6583 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6583 SmaI CCC'GGG Cuts at: 1872 2058 2651 3470 3910 1872 Size: 186 593 819 440 4545 Fragments arranged by size: 4545 819 593 440 186 SmlI C'TyrA_G Cuts at: 820 1017 1943 4687 5588 5850 820 Size: 197 926 2744 901 262 1553 Fragments arranged by size: 2744 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4374 493 Size: 3881 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6583 SphI G_CATG'C Cuts at: 1132 3403 3941 1132 Size: 2271 538 3774 Fragments arranged by size: 3774 2271 538 SspI AAT'ATT Cuts at: 5 52 3131 4507 5 Size: 47 3079 1376 2081 Fragments arranged by size: 3079 2081 1376 47 StuI AGG'CCT Cuts at: 1517 2770 1517 Size: 1253 5330 StyI C'CwwG_G Cuts at: 513 1065 2004 2203 3281 3288 3766 3872 4022 Size: 552 939 199 1078 7 478 106 150 Cuts at: 4022 513 Size: 3074 Fragments arranged by size: 3074 1078 939 552 478 199 150 106 7 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2514 2896 3833 4610 4627 4780 4965 Size: 1255 934 382 937 777 17 153 185 Cuts at: 4965 5410 325 Size: 445 1498 Fragments arranged by size: 1498 1255 937 934 777 445 382 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4829 6541 96 Size: 274 80 33 51 1443 2852 1712 138 Fragments arranged by size: 2852 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6543 6575 6543 Size: 32 6551 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6583 XbaI T'CTAG_A Cuts at: 3919 3919 Size: 6583 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6583 XmaI C'CCGG_G Cuts at: 1870 2056 2649 3468 3908 1870 Size: 186 593 819 440 4545 Fragments arranged by size: 4545 819 593 440 186 XmnI GAAnn'nnTTC Cuts at: 3132 4712 3132 Size: 1580 5003 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4391 276 Size: 53 83 186 936 2857 2468 Fragments arranged by size: 2857 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI