(Circular) (Six-base) MAPSORT of: pFN21AB6123.seq Check: 1879 from: 1 to: 6463 pFN21AB6123 6463 bp With 182 enzymes: SgfI * April 15, 2010 09:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4273 278 Size: 53 83 186 936 2737 2468 Fragments arranged by size: 2737 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2106 2791 3806 1937 Size: 169 685 1015 4594 Fragments arranged by size: 4594 1015 685 169 Acc65I G'GTAC_C Cuts at: 3784 3784 Size: 6463 AclI AA'CG_TT Cuts at: 1563 4590 4963 1563 Size: 3027 373 3063 Fragments arranged by size: 3063 3027 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2087 2156 2379 2502 2670 4526 5910 6178 Size: 455 69 223 123 168 1856 1384 268 Cuts at: 6178 1632 Size: 1917 Fragments arranged by size: 1917 1856 1384 455 268 223 168 123 69 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6266 AflIII A'CryG_T Cuts at: 1203 5362 6339 1203 Size: 4159 977 1327 Fragments arranged by size: 4159 1327 977 AgeI A'CCGG_T Cuts at: 5294 5294 Size: 6463 AhdI GACnn_n'nnGTC Cuts at: 5192 5192 Size: 6463 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6463 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3102 3106 1184 Size: 1918 4 4541 Fragments arranged by size: 4541 1918 4 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2108 2140 2108 Size: 32 6431 AlwNI CAG_nnn'CTG Cuts at: 4206 5778 4206 Size: 1572 4891 ApaLI G'TGCA_C Cuts at: 2021 2536 4520 5676 2021 Size: 515 1984 1156 2808 Fragments arranged by size: 2808 1984 1156 515 ApoI r'AATT_y Cuts at: 1493 2385 2701 2728 2885 3772 3998 4150 6259 Size: 892 316 27 157 887 226 152 2109 Cuts at: 6259 1493 Size: 1697 Fragments arranged by size: 2109 1697 892 887 316 226 157 152 27 AseI AT'TA_AT Cuts at: 160 1032 5017 160 Size: 872 3985 1606 Fragments arranged by size: 3985 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6463 AvaI C'yCGr_G Cuts at: 1870 1943 3788 1870 Size: 73 1845 4545 Fragments arranged by size: 4545 1845 73 BamHI G'GATC_C Cuts at: 3793 4160 3793 Size: 367 6096 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2084 3784 5239 618 Size: 325 217 627 297 1700 1455 1842 Fragments arranged by size: 1842 1700 1455 627 325 297 217 BanII G_rGCy'C Cuts at: 1391 2043 2316 3782 1391 Size: 652 273 1466 4072 Fragments arranged by size: 4072 1466 652 273 BbeI G_GCGC'C Cuts at: 2088 2088 Size: 6463 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3491 928 Size: 971 1592 3900 Fragments arranged by size: 3900 1592 971 BbvCI CC'TCA_GC Cuts at: 3426 3426 Size: 6463 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2716 2750 4654 4688 1903 Size: 34 779 34 1904 34 3678 Fragments arranged by size: 3678 1904 779 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3374 4355 5565 3374 Size: 981 1210 4272 Fragments arranged by size: 4272 1210 981 BclI T'GATC_A Cuts at: 1548 1548 Size: 6463 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2993 3009 5074 Size: 107 122 71 631 605 1321 16 2065 Cuts at: 5074 136 Size: 1525 Fragments arranged by size: 2065 1525 1321 631 605 122 107 71 16 BglII A'GATC_T Cuts at: 2815 3462 6458 2815 Size: 647 2996 2820 Fragments arranged by size: 2996 2820 647 BlpI GC'TnA_GC Cuts at: 3880 3880 Size: 6463 Bme1580I G_kGCm'C Cuts at: 1790 2025 2540 3680 4524 5680 1790 Size: 235 515 1140 844 1156 2573 Fragments arranged by size: 2573 1156 1140 844 515 235 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6463 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4194 5152 442 Size: 416 53 83 399 24 2777 958 1753 Fragments arranged by size: 2777 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6463 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2385 5123 1239 Size: 1146 2738 2579 Fragments arranged by size: 2738 2579 1146 Bpu10I CC'TnA_GC Cuts at: 3426 3426 Size: 6463 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2201 3582 4588 5453 5751 2201 Size: 1381 1006 865 298 2913 Fragments arranged by size: 2913 1381 1006 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3689 5126 882 Size: 617 2190 1437 2219 Fragments arranged by size: 2219 2190 1437 617 BsaAI yAC'GTr Cuts at: 493 1204 4254 493 Size: 711 3050 2702 Fragments arranged by size: 3050 2702 711 BsaBI GATnn'nnATC Cuts at: 2196 2970 6457 2196 Size: 774 3487 2202 Fragments arranged by size: 3487 2202 774 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2085 4270 4652 5287 Size: 53 83 186 936 552 2185 382 635 Cuts at: 5287 275 Size: 1451 Fragments arranged by size: 2185 1451 936 635 552 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4896 5294 5568 5715 6126 4896 Size: 398 274 147 411 5233 Fragments arranged by size: 5233 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2358 2388 1178 Size: 30 165 30 955 30 5253 Fragments arranged by size: 5253 955 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2125 2535 1186 Size: 939 410 5114 Fragments arranged by size: 5114 939 410 BseYI C'CCAG_C Cuts at: 5666 6162 5666 Size: 496 5967 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5890 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3913 4674 5702 1540 Size: 275 186 1912 761 1028 2301 Fragments arranged by size: 2301 1912 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2025 2540 3758 4524 4609 5680 2025 Size: 515 1218 766 85 1071 2808 Fragments arranged by size: 2808 1218 1071 766 515 85 BsmI GAATG_Cn' Cuts at: 2076 2728 3355 3974 4067 2076 Size: 652 627 619 93 4472 Fragments arranged by size: 4472 652 627 619 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2325 2325 Size: 6463 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2025 2043 2316 2540 3680 3758 3782 Size: 399 235 18 273 224 1140 78 24 Cuts at: 3782 4524 4609 5680 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1140 1071 742 399 273 235 224 85 78 24 18 BspEI T'CCGG_A Cuts at: 6126 6126 Size: 6463 BspHI T'CATG_A Cuts at: 3033 4352 6082 3033 Size: 1319 1730 3414 Fragments arranged by size: 3414 1730 1319 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3818 844 Size: 2974 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4350 1394 Size: 429 2527 3507 Fragments arranged by size: 3507 2527 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4958 5132 65 Size: 1183 3710 174 1396 Fragments arranged by size: 3710 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2129 5107 5294 1700 Size: 110 319 2978 187 2869 Fragments arranged by size: 2978 2869 319 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6463 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6463 BssSI C'ACGA_G Cuts at: 3102 4523 5535 3102 Size: 1421 1012 4030 Fragments arranged by size: 4030 1421 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6463 BstBI TT'CG_AA Cuts at: 5267 5267 Size: 6463 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6463 BstYI r'GATC_y Cuts at: 1972 2185 2815 3271 3462 3793 4160 4545 4562 Size: 213 630 456 191 331 367 385 17 Cuts at: 4562 6014 6100 6112 6458 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 630 456 385 367 346 331 213 191 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2976 2976 Size: 6463 BtgI C'CryG_G Cuts at: 513 1065 2004 3017 3672 513 Size: 552 939 1013 655 3304 Fragments arranged by size: 3304 1013 939 655 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5724 Fragments arranged by size: 5724 683 56 BtsI GCAGTG_nn' Cuts at: 2634 3235 3589 3979 4773 4793 2634 Size: 601 354 390 794 20 4304 Fragments arranged by size: 4304 794 601 390 354 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6463 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6428 DraI TTT'AAA Cuts at: 3767 3767 Size: 6463 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5470 809 Size: 507 4154 1802 Fragments arranged by size: 4154 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2633 3910 4799 Size: 54 1425 50 275 18 803 1277 889 Cuts at: 4799 6428 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1277 889 803 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3910 1537 Size: 275 2098 4090 Fragments arranged by size: 4090 2098 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2586 3708 4393 1353 Size: 1233 1122 685 3423 Fragments arranged by size: 3423 1233 1122 685 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2655 3011 5050 5424 5570 387 Size: 1064 138 1066 356 2039 374 146 1280 Fragments arranged by size: 2039 1280 1066 1064 374 356 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2087 2156 2379 2385 2502 2670 4526 Size: 393 455 69 223 6 117 168 1856 Cuts at: 4526 5123 5910 6178 1239 Size: 597 787 268 1524 Fragments arranged by size: 1856 1524 787 597 455 393 268 223 168 117 69 6 EcoO109I rG'GnC_Cy Cuts at: 3109 3109 Size: 6463 EcoRI G'AATT_C Cuts at: 2728 3772 4150 2728 Size: 1044 378 5041 Fragments arranged by size: 5041 1044 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2706 2706 Size: 6463 FspI TGC'GCA Cuts at: 4969 4969 Size: 6463 HaeII r_GCGC'y Cuts at: 1678 2088 5610 1678 Size: 410 3522 2531 Fragments arranged by size: 3522 2531 410 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2358 2390 2603 2635 3052 Size: 32 135 32 786 32 213 32 417 Cuts at: 3052 3084 3256 3273 3288 3305 3718 3750 5104 Size: 32 172 17 15 17 413 32 1354 Cuts at: 5104 5136 5178 5210 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1354 786 417 413 213 172 135 42 32 32 32 32 32 32 32 32 17 17 15 HincII GTy'rAC Cuts at: 669 1657 1938 2792 3807 4053 669 Size: 988 281 854 1015 246 3079 Fragments arranged by size: 3079 1015 988 854 281 246 HindIII A'AGCT_T Cuts at: 748 2688 748 Size: 1940 4523 HpaI GTT'AAC Cuts at: 4053 4053 Size: 6463 KasI G'GCGC_C Cuts at: 2084 2084 Size: 6463 KpnI G_GTAC'C Cuts at: 3788 3788 Size: 6463 MfeI C'AATT_G Cuts at: 2295 4062 2295 Size: 1767 4696 MluI A'CGCG_T Cuts at: 6339 6339 Size: 6463 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3038 3173 5577 5761 1305 Size: 1733 135 2404 184 2007 Fragments arranged by size: 2404 2007 1733 184 135 MscI TGG'CCA Cuts at: 10 64 1489 1832 2635 10 Size: 54 1425 343 803 3838 Fragments arranged by size: 3838 1425 803 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 4421 4780 4939 518 Size: 648 843 2412 359 159 2042 Fragments arranged by size: 2412 2042 843 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2100 3879 4556 5704 5949 1606 Size: 494 1779 677 1148 245 2120 Fragments arranged by size: 2120 1779 1148 677 494 245 NaeI GCC'GGC Cuts at: 2131 2131 Size: 6463 NarI GG'CG_CC Cuts at: 2085 2085 Size: 6463 NcoI C'CATG_G Cuts at: 513 1065 2004 3017 3672 513 Size: 552 939 1013 655 3304 Fragments arranged by size: 3304 1013 939 655 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6463 NgoMIV G'CCGG_C Cuts at: 2129 2129 Size: 6463 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6463 NotI GC'GGCC_GC Cuts at: 3910 3910 Size: 6463 NspI r_CATG'y Cuts at: 1132 1229 3083 3821 5366 1132 Size: 97 1854 738 1545 2229 Fragments arranged by size: 2229 1854 1545 738 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6463 PciI A'CATG_T Cuts at: 5362 5362 Size: 6463 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6463 PfoI T'CCnGG_A Cuts at: 2112 2112 Size: 6463 PmeI GTTT'AAAC Cuts at: 3767 3767 Size: 6463 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4532 4564 6045 6077 4532 Size: 32 1481 32 4918 Fragments arranged by size: 4918 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3109 3109 Size: 6463 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6463 PsiI TTA'TAA Cuts at: 2454 4033 2454 Size: 1579 4884 PstI C_TGCA'G Cuts at: 830 2629 3559 3815 830 Size: 1799 930 256 3478 Fragments arranged by size: 3478 1799 930 256 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6463 SalI G'TCGA_C Cuts at: 1936 2790 3805 1936 Size: 854 1015 4594 Fragments arranged by size: 4594 1015 854 SapI GCTCTTCn'nnn_ Cuts at: 2586 2586 Size: 6463 SbfI CC_TGCA'GG Cuts at: 3815 3815 Size: 6463 ScaI AGT'ACT Cuts at: 4711 6423 4711 Size: 1712 4751 SfcI C'TryA_G Cuts at: 826 1045 2625 3555 3811 4946 5627 5818 826 Size: 219 1580 930 256 1135 681 191 1471 Fragments arranged by size: 1580 1471 1135 930 681 256 219 191 SfoI GGC'GCC Cuts at: 2086 2086 Size: 6463 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6463 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6463 SmaI CCC'GGG Cuts at: 1872 3790 1872 Size: 1918 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2180 3597 4567 5468 5730 820 Size: 197 926 237 1417 970 901 262 1553 Fragments arranged by size: 1553 1417 970 926 901 262 237 197 SnaBI TAC'GTA Cuts at: 493 4254 493 Size: 3761 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6463 SphI G_CATG'C Cuts at: 1132 3083 3821 1132 Size: 1951 738 3774 Fragments arranged by size: 3774 1951 738 SspI AAT'ATT Cuts at: 5 52 4387 5 Size: 47 4335 2081 Fragments arranged by size: 4335 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6463 StyI C'CwwG_G Cuts at: 513 1065 2004 2960 3017 3672 3902 513 Size: 552 939 956 57 655 230 3074 Fragments arranged by size: 3074 956 939 655 552 230 57 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2683 4490 4507 4660 4845 5290 325 Size: 1255 1103 1807 17 153 185 445 1498 Fragments arranged by size: 1807 1498 1255 1103 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4709 6421 96 Size: 274 80 33 51 1443 2732 1712 138 Fragments arranged by size: 2732 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6423 6455 6423 Size: 32 6431 Tth111I GACn'n_nGTC Cuts at: 1316 2757 3495 1316 Size: 1441 738 4284 Fragments arranged by size: 4284 1441 738 XbaI T'CTAG_A Cuts at: 3799 3799 Size: 6463 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6463 XmaI C'CCGG_G Cuts at: 1870 3788 1870 Size: 1918 4545 XmnI GAAnn'nnTTC Cuts at: 4592 4592 Size: 6463 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4271 276 Size: 53 83 186 936 2737 2468 Fragments arranged by size: 2737 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BfrBI BplI BsiWI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI XcmI