(Circular) (Six-base) MAPSORT of: pFN21AB6119.seq Check: 2168 from: 1 to: 6574 pFN21AB6119 6574 bp With 182 enzymes: SgfI * April 15, 2010 09:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2823 4384 278 Size: 53 83 186 936 1287 1561 2468 Fragments arranged by size: 2468 1561 1287 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3917 1937 Size: 1980 4594 Acc65I G'GTAC_C Cuts at: 3895 3895 Size: 6574 AclI AA'CG_TT Cuts at: 1563 4701 5074 1563 Size: 3138 373 3063 Fragments arranged by size: 3138 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2058 4637 6021 6289 1632 Size: 426 2579 1384 268 1917 Fragments arranged by size: 2579 1917 1384 426 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6377 AflIII A'CryG_T Cuts at: 1203 2017 5473 6450 1203 Size: 814 3456 977 1327 Fragments arranged by size: 3456 1327 977 814 AgeI A'CCGG_T Cuts at: 5405 5405 Size: 6574 AhdI GACnn_n'nnGTC Cuts at: 5303 5303 Size: 6574 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6574 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6574 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3746 3778 3746 Size: 32 6542 AlwNI CAG_nnn'CTG Cuts at: 4317 5889 4317 Size: 1572 5002 ApaLI G'TGCA_C Cuts at: 4631 5787 4631 Size: 1156 5418 ApoI r'AATT_y Cuts at: 1493 2080 2139 2204 2685 2789 3883 4109 4261 Size: 587 59 65 481 104 1094 226 152 Cuts at: 4261 6370 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1094 587 481 226 152 104 65 59 AseI AT'TA_AT Cuts at: 160 1032 5128 160 Size: 872 4096 1606 Fragments arranged by size: 4096 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6574 AvaI C'yCGr_G Cuts at: 1870 1943 3899 1870 Size: 73 1956 4545 Fragments arranged by size: 4545 1956 73 BamHI G'GATC_C Cuts at: 2590 3904 4271 2590 Size: 1314 367 4893 Fragments arranged by size: 4893 1314 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3626 3735 3895 5350 618 Size: 325 217 627 1839 109 160 1455 1842 Fragments arranged by size: 1842 1839 1455 627 325 217 160 109 BanII G_rGCy'C Cuts at: 1391 2046 2750 3764 3893 1391 Size: 655 704 1014 129 4072 Fragments arranged by size: 4072 1014 704 655 129 BbeI G_GCGC'C Cuts at: 3739 3739 Size: 6574 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2220 2309 928 Size: 971 321 89 5193 Fragments arranged by size: 5193 971 321 89 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4765 4799 1903 Size: 34 2828 34 3678 Fragments arranged by size: 3678 2828 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2044 3844 4466 5676 2044 Size: 1800 622 1210 2942 Fragments arranged by size: 2942 1800 1210 622 BclI T'GATC_A Cuts at: 1548 3799 1548 Size: 2251 4323 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5185 136 Size: 107 122 71 631 605 3513 1525 Fragments arranged by size: 3513 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2518 6569 2518 Size: 4051 2523 BlpI GC'TnA_GC Cuts at: 3777 3991 3777 Size: 214 6360 Bme1580I G_kGCm'C Cuts at: 1790 3631 4635 5791 1790 Size: 1841 1004 1156 2573 Fragments arranged by size: 2573 1841 1156 1004 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6574 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3766 4305 5263 Size: 416 53 83 399 24 2349 539 958 Cuts at: 5263 442 Size: 1753 Fragments arranged by size: 2349 1753 958 539 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6574 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2433 3360 5234 1239 Size: 1194 927 1874 2579 Fragments arranged by size: 2579 1874 1194 927 Bpu10I CC'TnA_GC Cuts at: 2652 2652 Size: 6574 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4699 5564 5862 4699 Size: 865 298 5411 Fragments arranged by size: 5411 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2609 5237 882 Size: 617 1110 2628 2219 Fragments arranged by size: 2628 2219 1110 617 BsaAI yAC'GTr Cuts at: 493 1204 3656 4365 493 Size: 711 2452 709 2702 Fragments arranged by size: 2702 2452 711 709 BsaBI GATnn'nnATC Cuts at: 2991 6568 2991 Size: 3577 2997 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2820 3736 4381 4763 Size: 53 83 186 936 1287 916 645 382 Cuts at: 4763 5398 275 Size: 635 1451 Fragments arranged by size: 1451 1287 936 916 645 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2839 5007 5405 5679 5826 6237 2839 Size: 2168 398 274 147 411 3176 Fragments arranged by size: 3176 2168 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3746 3776 1178 Size: 30 165 30 2343 30 3976 Fragments arranged by size: 3976 2343 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3522 3540 3597 3636 1186 Size: 2336 18 57 39 4124 Fragments arranged by size: 4124 2336 57 39 18 BseYI C'CCAG_C Cuts at: 5777 6273 5777 Size: 496 6078 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6001 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4024 4785 5813 1540 Size: 275 186 2023 761 1028 2301 Fragments arranged by size: 2301 2023 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4635 4720 5791 4635 Size: 85 1071 5418 Fragments arranged by size: 5418 1071 85 BsmI GAATG_Cn' Cuts at: 4085 4178 4085 Size: 93 6481 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2046 2750 3631 3764 3893 4635 4720 Size: 399 256 704 881 133 129 742 85 Cuts at: 4720 5791 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 881 742 704 399 256 133 129 85 BspEI T'CCGG_A Cuts at: 6237 6237 Size: 6574 BspHI T'CATG_A Cuts at: 2298 2680 4463 6193 2298 Size: 382 1783 1730 2679 Fragments arranged by size: 2679 1783 1730 382 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3929 844 Size: 3085 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3787 4461 1394 Size: 429 1964 674 3507 Fragments arranged by size: 3507 1964 674 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2090 3771 5069 5243 65 Size: 1183 842 1681 1298 174 1396 Fragments arranged by size: 1681 1396 1298 1183 842 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3566 5218 5405 1700 Size: 110 1756 1652 187 2869 Fragments arranged by size: 2869 1756 1652 187 110 BsrGI T'GTAC_A Cuts at: 96 2636 96 Size: 2540 4034 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6574 BssSI C'ACGA_G Cuts at: 4634 5646 4634 Size: 1012 5562 BstAPI GCAn_nnn'nTGC Cuts at: 794 3518 794 Size: 2724 3850 BstBI TT'CG_AA Cuts at: 5378 5378 Size: 6574 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6574 BstYI r'GATC_y Cuts at: 1972 2508 2518 2572 2590 2986 3451 3904 4271 Size: 536 10 54 18 396 465 453 367 Cuts at: 4271 4656 4673 6125 6211 6223 6569 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 536 465 453 396 385 367 346 86 54 18 17 12 10 Bsu36I CC'TnA_GG Cuts at: 2997 2997 Size: 6574 BtgI C'CryG_G Cuts at: 513 1065 2004 2046 3556 3646 3673 513 Size: 552 939 42 1510 90 27 3414 Fragments arranged by size: 3414 1510 939 552 90 42 27 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5835 Fragments arranged by size: 5835 683 56 BtsI GCAGTG_nn' Cuts at: 4090 4884 4904 4090 Size: 794 20 5760 Fragments arranged by size: 5760 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6574 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6539 DraI TTT'AAA Cuts at: 2199 2245 3878 2199 Size: 46 1633 4895 Fragments arranged by size: 4895 1633 46 DraIII CAC_nnn'GTG Cuts at: 2168 2168 Size: 6574 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5581 809 Size: 507 4265 1802 Fragments arranged by size: 4265 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3670 4021 4910 Size: 54 1425 50 275 18 1840 351 889 Cuts at: 4910 6539 8 Size: 1629 43 Fragments arranged by size: 1840 1629 1425 889 351 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4021 1537 Size: 275 2209 4090 Fragments arranged by size: 4090 2209 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2034 3051 4504 1353 Size: 681 1017 1453 3423 Fragments arranged by size: 3423 1453 1017 681 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5161 5535 5681 387 Size: 1064 138 3572 374 146 1280 Fragments arranged by size: 3572 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2058 2433 3360 4637 5234 6021 6289 Size: 393 426 375 927 1277 597 787 268 Cuts at: 6289 1239 Size: 1524 Fragments arranged by size: 1524 1277 927 787 597 426 393 375 268 EcoO109I rG'GnC_Cy Cuts at: 3587 3587 Size: 6574 EcoRI G'AATT_C Cuts at: 2204 3883 4261 2204 Size: 1679 378 4517 Fragments arranged by size: 4517 1679 378 EcoRV GAT'ATC Cuts at: 3371 3371 Size: 6574 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2401 2401 Size: 6574 FspI TGC'GCA Cuts at: 5080 5080 Size: 6574 HaeII r_GCGC'y Cuts at: 1678 3739 5721 1678 Size: 2061 1982 2531 Fragments arranged by size: 2531 2061 1982 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3571 3603 3733 3765 5215 Size: 32 135 32 1999 32 130 32 1450 Cuts at: 5215 5247 5289 5321 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1999 1450 135 130 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3918 4164 669 Size: 988 281 1980 246 3079 Fragments arranged by size: 3079 1980 988 281 246 HindIII A'AGCT_T Cuts at: 748 2193 748 Size: 1445 5129 HpaI GTT'AAC Cuts at: 4164 4164 Size: 6574 KasI G'GCGC_C Cuts at: 3735 3735 Size: 6574 KpnI G_GTAC'C Cuts at: 3899 3899 Size: 6574 MfeI C'AATT_G Cuts at: 4173 4173 Size: 6574 MluI A'CGCG_T Cuts at: 6450 6450 Size: 6574 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2935 3353 3463 5688 5872 1305 Size: 1630 418 110 2225 184 2007 Fragments arranged by size: 2225 2007 1630 418 184 110 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4752 Fragments arranged by size: 4752 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2022 2706 3138 4532 4891 5050 518 Size: 648 856 684 432 1394 359 159 2042 Fragments arranged by size: 2042 1394 856 684 648 432 359 159 MspA1I CmG'CkG Cuts at: 1606 2900 3558 3648 3675 3990 4667 5815 6060 Size: 1294 658 90 27 315 677 1148 245 Cuts at: 6060 1606 Size: 2120 Fragments arranged by size: 2120 1294 1148 677 658 315 245 90 27 NarI GG'CG_CC Cuts at: 3736 3736 Size: 6574 NcoI C'CATG_G Cuts at: 513 1065 2004 2046 513 Size: 552 939 42 5041 Fragments arranged by size: 5041 939 552 42 NdeI CA'TA_TG Cuts at: 387 3318 387 Size: 2931 3643 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6574 NotI GC'GGCC_GC Cuts at: 4021 4021 Size: 6574 NspI r_CATG'y Cuts at: 1132 1229 2021 3932 5477 1132 Size: 97 792 1911 1545 2229 Fragments arranged by size: 2229 1911 1545 792 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6574 PciI A'CATG_T Cuts at: 2017 5473 2017 Size: 3456 3118 PflMI CCAn_nnn'nTGG Cuts at: 1108 2600 3461 1108 Size: 1492 861 4221 Fragments arranged by size: 4221 1492 861 PfoI T'CCnGG_A Cuts at: 3589 3589 Size: 6574 PmeI GTTT'AAAC Cuts at: 3878 3878 Size: 6574 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3746 3778 4643 4675 6156 6188 3746 Size: 32 865 32 1481 32 4132 Fragments arranged by size: 4132 1481 865 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3587 3587 Size: 6574 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6574 PsiI TTA'TAA Cuts at: 4144 4144 Size: 6574 PstI C_TGCA'G Cuts at: 830 2065 3926 830 Size: 1235 1861 3478 Fragments arranged by size: 3478 1861 1235 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6574 PvuII CAG'CTG Cuts at: 2900 2900 Size: 6574 SacII CC_GC'GG Cuts at: 3559 3649 3676 3559 Size: 90 27 6457 Fragments arranged by size: 6457 90 27 SalI G'TCGA_C Cuts at: 1936 3916 1936 Size: 1980 4594 SapI GCTCTTCn'nnn_ Cuts at: 2034 2034 Size: 6574 SbfI CC_TGCA'GG Cuts at: 3926 3926 Size: 6574 ScaI AGT'ACT Cuts at: 4822 6534 4822 Size: 1712 4862 SfcI C'TryA_G Cuts at: 826 1045 2010 2061 2979 3922 5057 5738 5929 Size: 219 965 51 918 943 1135 681 191 Cuts at: 5929 826 Size: 1471 Fragments arranged by size: 1471 1135 965 943 918 681 219 191 51 SfoI GGC'GCC Cuts at: 3737 3737 Size: 6574 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6574 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6574 SmaI CCC'GGG Cuts at: 1872 3901 1872 Size: 2029 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4678 5579 5841 820 Size: 197 926 2735 901 262 1553 Fragments arranged by size: 2735 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4365 493 Size: 3872 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6574 SphI G_CATG'C Cuts at: 1132 3932 1132 Size: 2800 3774 SspI AAT'ATT Cuts at: 5 52 3205 4498 5 Size: 47 3153 1293 2081 Fragments arranged by size: 3153 2081 1293 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6574 StyI C'CwwG_G Cuts at: 513 1065 2004 2046 2769 3790 4013 513 Size: 552 939 42 723 1021 223 3074 Fragments arranged by size: 3074 1021 939 723 552 223 42 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4601 4618 4771 4956 5401 325 Size: 1255 3021 17 153 185 445 1498 Fragments arranged by size: 3021 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2636 4820 6532 Size: 274 80 33 51 1443 659 2184 1712 Cuts at: 6532 96 Size: 138 Fragments arranged by size: 2184 1712 1443 659 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6534 6566 6534 Size: 32 6542 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6574 XbaI T'CTAG_A Cuts at: 3910 3910 Size: 6574 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3434 3434 Size: 6574 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6574 XmaI C'CCGG_G Cuts at: 1870 3899 1870 Size: 2029 4545 XmnI GAAnn'nnTTC Cuts at: 2082 2538 2778 3474 4703 2082 Size: 456 240 696 1229 3953 Fragments arranged by size: 3953 1229 696 456 240 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2821 4382 276 Size: 53 83 186 936 1287 1561 2468 Fragments arranged by size: 2468 1561 1287 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I EcoICRI EcoNI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SanDI SexAI SfiI SrfI SwaI