(Circular) (Six-base) MAPSORT of: pFN21AB6118.seq Check: 8587 from: 1 to: 6343 pFN21AB6118 6343 bp With 182 enzymes: SgfI * April 15, 2010 09:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2398 2398 Size: 6343 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4153 278 Size: 53 83 186 936 2617 2468 Fragments arranged by size: 2617 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2532 3686 1937 Size: 595 1154 4594 Fragments arranged by size: 4594 1154 595 Acc65I G'GTAC_C Cuts at: 3664 3664 Size: 6343 AclI AA'CG_TT Cuts at: 1563 4470 4843 1563 Size: 2907 373 3063 Fragments arranged by size: 3063 2907 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4406 5790 6058 1632 Size: 2774 1384 268 1917 Fragments arranged by size: 2774 1917 1384 268 AfeI AGC'GCT Cuts at: 2304 2304 Size: 6343 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6146 AflIII A'CryG_T Cuts at: 1203 5242 6219 1203 Size: 4039 977 1327 Fragments arranged by size: 4039 1327 977 AgeI A'CCGG_T Cuts at: 5174 5174 Size: 6343 AhdI GACnn_n'nnGTC Cuts at: 5072 5072 Size: 6343 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6343 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3048 1184 Size: 1864 4479 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2645 2677 2645 Size: 32 6311 AlwNI CAG_nnn'CTG Cuts at: 2185 2353 3363 4086 5658 2185 Size: 168 1010 723 1572 2870 Fragments arranged by size: 2870 1572 1010 723 168 ApaI G_GGCC'C Cuts at: 2994 3232 2994 Size: 238 6105 ApaLI G'TGCA_C Cuts at: 2411 2498 4400 5556 2411 Size: 87 1902 1156 3198 Fragments arranged by size: 3198 1902 1156 87 ApoI r'AATT_y Cuts at: 1493 3029 3632 3652 3878 4030 6139 1493 Size: 1536 603 20 226 152 2109 1697 Fragments arranged by size: 2109 1697 1536 603 226 152 20 AseI AT'TA_AT Cuts at: 160 1032 4897 160 Size: 872 3865 1606 Fragments arranged by size: 3865 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6343 AvaI C'yCGr_G Cuts at: 1870 1943 2053 2986 3611 3668 1870 Size: 73 110 933 625 57 4545 Fragments arranged by size: 4545 933 625 110 73 57 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2916 2949 2916 Size: 33 6310 BamHI G'GATC_C Cuts at: 2217 3673 4040 2217 Size: 1456 367 4520 Fragments arranged by size: 4520 1456 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2057 2388 2698 3018 3161 Size: 325 217 627 270 331 310 320 143 Cuts at: 3161 3664 5119 618 Size: 503 1455 1842 Fragments arranged by size: 1842 1455 627 503 331 325 320 310 270 217 143 BanII G_rGCy'C Cuts at: 1391 2597 2751 2994 3232 3662 1391 Size: 1206 154 243 238 430 4072 Fragments arranged by size: 4072 1206 430 243 238 154 BbeI G_GCGC'C Cuts at: 2061 2392 2061 Size: 331 6012 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3456 928 Size: 971 1557 3815 Fragments arranged by size: 3815 1557 971 BbvCI CC'TCA_GC Cuts at: 3130 3475 3130 Size: 345 5998 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2497 2531 2534 2568 4534 4568 1903 Size: 34 560 34 3 34 1966 34 3678 Fragments arranged by size: 3678 1966 560 34 34 34 34 3 BciVI GTATCCnnnnn_n' Cuts at: 4235 5445 4235 Size: 1210 5133 BclI T'GATC_A Cuts at: 1548 1548 Size: 6343 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2386 3016 3434 Size: 107 122 71 631 605 714 630 418 Cuts at: 3434 4954 136 Size: 1520 1525 Fragments arranged by size: 1525 1520 714 631 630 605 418 122 107 71 BglII A'GATC_T Cuts at: 6338 6338 Size: 6343 BlpI GC'TnA_GC Cuts at: 2295 3760 2295 Size: 1465 4878 Bme1580I G_kGCm'C Cuts at: 1790 2415 2502 2696 2703 2994 3021 3166 3174 Size: 625 87 194 7 291 27 145 8 Cuts at: 3174 3232 4404 5560 1790 Size: 58 1172 1156 2573 Fragments arranged by size: 2573 1172 1156 625 291 194 145 87 58 27 8 7 BmgBI CAC'GTC Cuts at: 1318 3244 1318 Size: 1926 4417 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3300 3373 4074 Size: 416 53 83 399 24 1883 73 701 Cuts at: 4074 5032 442 Size: 958 1753 Fragments arranged by size: 1883 1753 958 701 416 399 83 73 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6343 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2741 5003 1239 Size: 1502 2262 2579 Fragments arranged by size: 2579 2262 1502 Bpu10I CC'TnA_GC Cuts at: 3130 3475 3130 Size: 345 5998 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4468 5333 5631 4468 Size: 865 298 5180 Fragments arranged by size: 5180 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2057 3292 5006 882 Size: 617 558 1235 1714 2219 Fragments arranged by size: 2219 1714 1235 617 558 BsaAI yAC'GTr Cuts at: 493 1204 4134 493 Size: 711 2930 2702 Fragments arranged by size: 2930 2702 711 BsaBI GATnn'nnATC Cuts at: 6337 6337 Size: 6343 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2058 2261 2389 2474 Size: 53 83 186 936 525 203 128 85 Cuts at: 2474 4150 4532 5167 275 Size: 1676 382 635 1451 Fragments arranged by size: 1676 1451 936 635 525 382 203 186 128 85 83 53 BsaWI w'CCGG_w Cuts at: 2066 2586 2997 4776 5174 5448 5595 6006 2066 Size: 520 411 1779 398 274 147 411 2403 Fragments arranged by size: 2403 1779 520 411 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2078 2108 3191 3221 1178 Size: 30 165 30 675 30 1083 30 4300 Fragments arranged by size: 4300 1083 675 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2048 2345 2605 2701 3118 3194 3463 3520 Size: 862 297 260 96 417 76 269 57 Cuts at: 3520 1186 Size: 4009 Fragments arranged by size: 4009 862 417 297 269 260 96 76 57 BseYI C'CCAG_C Cuts at: 2178 2597 3499 3506 5546 6042 2178 Size: 419 902 7 2040 496 2479 Fragments arranged by size: 2479 2040 902 496 419 7 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2345 3285 1163 Size: 573 609 940 4221 Fragments arranged by size: 4221 940 609 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2419 3793 4554 5582 1540 Size: 275 186 418 1374 761 1028 2301 Fragments arranged by size: 2301 1374 1028 761 418 275 186 BsiHKAI G_wGCw'C Cuts at: 2415 2502 2751 2927 4404 4489 5560 2415 Size: 87 249 176 1477 85 1071 3198 Fragments arranged by size: 3198 1477 1071 249 176 87 85 BsmI GAATG_Cn' Cuts at: 3854 3947 3854 Size: 93 6250 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2415 2502 2597 2696 2703 2751 2927 Size: 399 625 87 95 99 7 48 176 Cuts at: 2927 2994 3021 3166 3174 3232 3662 4404 4489 Size: 67 27 145 8 58 430 742 85 Cuts at: 4489 5560 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 625 430 399 176 145 99 95 87 85 67 58 48 27 8 7 BspEI T'CCGG_A Cuts at: 2586 2997 6006 2586 Size: 411 3009 2923 Fragments arranged by size: 3009 2923 411 BspHI T'CATG_A Cuts at: 4232 5962 4232 Size: 1730 4613 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2398 2442 3698 844 Size: 1554 44 1256 3489 Fragments arranged by size: 3489 1554 1256 44 BsrBI CCG'CTC Cuts at: 1394 1823 2971 4230 1394 Size: 429 1148 1259 3507 Fragments arranged by size: 3507 1259 1148 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4838 5012 65 Size: 1183 3590 174 1396 Fragments arranged by size: 3590 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2418 4987 5174 1700 Size: 110 608 2569 187 2869 Fragments arranged by size: 2869 2569 608 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6343 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6343 BssSI C'ACGA_G Cuts at: 2098 2750 3486 4403 5415 2098 Size: 652 736 917 1012 3026 Fragments arranged by size: 3026 1012 917 736 652 BstAPI GCAn_nnn'nTGC Cuts at: 794 2353 3299 794 Size: 1559 946 3838 Fragments arranged by size: 3838 1559 946 BstBI TT'CG_AA Cuts at: 5147 5147 Size: 6343 BstEII G'GTnAC_C Cuts at: 1184 3415 1184 Size: 2231 4112 BstXI CCAn_nnnn'nTGG Cuts at: 2948 2948 Size: 6343 BstYI r'GATC_y Cuts at: 1972 2217 2349 2826 3673 4040 4425 4442 5894 Size: 245 132 477 847 367 385 17 1452 Cuts at: 5894 5980 5992 6338 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 847 477 385 367 346 245 132 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2564 2564 Size: 6343 BtgI C'CryG_G Cuts at: 513 1065 2004 3105 513 Size: 552 939 1101 3751 Fragments arranged by size: 3751 1101 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5604 Fragments arranged by size: 5604 683 56 BtsI GCAGTG_nn' Cuts at: 2110 2369 2925 3395 3859 4653 4673 2110 Size: 259 556 470 464 794 20 3780 Fragments arranged by size: 3780 794 556 470 464 259 20 ClaI AT'CG_AT Cuts at: 1554 3213 1554 Size: 1659 4684 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6308 DraI TTT'AAA Cuts at: 3647 3647 Size: 6343 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2071 5350 809 Size: 507 755 3279 1802 Fragments arranged by size: 3279 1802 755 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2416 3196 3790 Size: 54 1425 50 275 18 586 780 594 Cuts at: 3790 4679 6308 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 780 594 586 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2416 3790 1537 Size: 275 604 1374 4090 Fragments arranged by size: 4090 1374 604 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2115 2337 4273 1353 Size: 762 222 1936 3423 Fragments arranged by size: 3423 1936 762 222 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2290 4930 5304 5450 387 Size: 1064 138 701 2640 374 146 1280 Fragments arranged by size: 2640 1280 1064 701 374 146 138 EcoICRI GAG'CTC Cuts at: 2749 2749 Size: 6343 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2741 4406 5003 5790 6058 1239 Size: 393 1109 1665 597 787 268 1524 Fragments arranged by size: 1665 1524 1109 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2189 2327 2834 2189 Size: 138 507 5698 Fragments arranged by size: 5698 507 138 EcoO109I rG'GnC_Cy Cuts at: 2131 2990 3113 3228 2131 Size: 859 123 115 5246 Fragments arranged by size: 5246 859 123 115 EcoRI G'AATT_C Cuts at: 3632 3652 4030 3632 Size: 20 378 5945 Fragments arranged by size: 5945 378 20 FspI TGC'GCA Cuts at: 4849 4849 Size: 6343 HaeII r_GCGC'y Cuts at: 1678 2061 2295 2306 2392 2797 5490 1678 Size: 383 234 11 86 405 2693 2531 Fragments arranged by size: 2693 2531 405 383 234 86 11 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4984 5016 5058 5090 1373 Size: 32 135 32 3412 32 42 32 2626 Fragments arranged by size: 3412 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3687 3933 669 Size: 988 281 1749 246 3079 Fragments arranged by size: 3079 1749 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6343 HpaI GTT'AAC Cuts at: 3933 3933 Size: 6343 KasI G'GCGC_C Cuts at: 2057 2388 2057 Size: 331 6012 KpnI G_GTAC'C Cuts at: 3668 3668 Size: 6343 MfeI C'AATT_G Cuts at: 3942 3942 Size: 6343 MluI A'CGCG_T Cuts at: 6219 6219 Size: 6343 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2100 2664 5457 5641 1305 Size: 795 564 2793 184 2007 Fragments arranged by size: 2793 2007 795 564 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3198 10 Size: 54 1425 343 1366 3155 Fragments arranged by size: 3155 1425 1366 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3184 4301 4660 4819 518 Size: 648 2018 1117 359 159 2042 Fragments arranged by size: 2042 2018 1117 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2236 2359 2578 3134 3759 4436 5584 5829 Size: 630 123 219 556 625 677 1148 245 Cuts at: 5829 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 630 625 556 245 219 123 NarI GG'CG_CC Cuts at: 2058 2389 2058 Size: 331 6012 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4852 Fragments arranged by size: 4852 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6343 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6343 NotI GC'GGCC_GC Cuts at: 3790 3790 Size: 6343 NspI r_CATG'y Cuts at: 1132 1229 3586 3701 5246 1132 Size: 97 2357 115 1545 2229 Fragments arranged by size: 2357 2229 1545 115 97 PasI CC'CwG_GG Cuts at: 1348 2168 2468 3167 3596 1348 Size: 820 300 699 429 4095 Fragments arranged by size: 4095 820 699 429 300 PciI A'CATG_T Cuts at: 5242 5242 Size: 6343 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6343 PmeI GTTT'AAAC Cuts at: 3647 3647 Size: 6343 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4412 4444 5925 5957 4412 Size: 32 1481 32 4798 Fragments arranged by size: 4798 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2131 2131 Size: 6343 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6343 PsiI TTA'TAA Cuts at: 3913 3913 Size: 6343 PspOMI G'GGCC_C Cuts at: 2990 3228 2990 Size: 238 6105 PstI C_TGCA'G Cuts at: 830 2190 2328 2358 2376 2444 3320 3695 830 Size: 1360 138 30 18 68 876 375 3478 Fragments arranged by size: 3478 1360 876 375 138 68 30 18 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6343 PvuII CAG'CTG Cuts at: 2359 2578 3134 2359 Size: 219 556 5568 Fragments arranged by size: 5568 556 219 SacI G_AGCT'C Cuts at: 2751 2751 Size: 6343 SalI G'TCGA_C Cuts at: 1936 3685 1936 Size: 1749 4594 SapI GCTCTTCn'nnn_ Cuts at: 2337 2337 Size: 6343 SbfI CC_TGCA'GG Cuts at: 2190 2328 3695 2190 Size: 138 1367 4838 Fragments arranged by size: 4838 1367 138 ScaI AGT'ACT Cuts at: 4591 6303 4591 Size: 1712 4631 SfcI C'TryA_G Cuts at: 826 1045 2080 2186 2324 2354 2372 2440 2834 Size: 219 1035 106 138 30 18 68 394 Cuts at: 2834 3316 3691 4826 5507 5698 826 Size: 482 375 1135 681 191 1471 Fragments arranged by size: 1471 1135 1035 681 482 394 375 219 191 138 106 68 30 18 SfoI GGC'GCC Cuts at: 2059 2390 2059 Size: 331 6012 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6343 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6343 SmaI CCC'GGG Cuts at: 1872 2988 3613 3670 1872 Size: 1116 625 57 4545 Fragments arranged by size: 4545 1116 625 57 SmlI C'TyrA_G Cuts at: 820 1017 1943 4447 5348 5610 820 Size: 197 926 2504 901 262 1553 Fragments arranged by size: 2504 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4134 493 Size: 3641 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6343 SphI G_CATG'C Cuts at: 1132 3701 1132 Size: 2569 3774 SspI AAT'ATT Cuts at: 5 52 4267 5 Size: 47 4215 2081 Fragments arranged by size: 4215 2081 47 StuI AGG'CCT Cuts at: 1517 3006 3255 3428 1517 Size: 1489 249 173 4432 Fragments arranged by size: 4432 1489 249 173 StyI C'CwwG_G Cuts at: 513 1065 2004 2286 3782 513 Size: 552 939 282 1496 3074 Fragments arranged by size: 3074 1496 939 552 282 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3319 4370 4387 4540 4725 5170 325 Size: 1255 1739 1051 17 153 185 445 1498 Fragments arranged by size: 1739 1498 1255 1051 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2878 4589 6301 Size: 274 80 33 51 1443 901 1711 1712 Cuts at: 6301 96 Size: 138 Fragments arranged by size: 1712 1711 1443 901 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2078 2110 3191 3223 3365 3397 6303 6335 2078 Size: 32 1081 32 142 32 2906 32 2086 Fragments arranged by size: 2906 2086 1081 142 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2204 1316 Size: 888 5455 XbaI T'CTAG_A Cuts at: 3679 3679 Size: 6343 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2647 2647 Size: 6343 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6343 XmaI C'CCGG_G Cuts at: 1870 2986 3611 3668 1870 Size: 1116 625 57 4545 Fragments arranged by size: 4545 1116 625 57 XmnI GAAnn'nnTTC Cuts at: 4472 4472 Size: 6343 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4151 276 Size: 53 83 186 936 2617 2468 Fragments arranged by size: 2617 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacII SanDI SexAI SfiI SrfI SwaI