Restriction map for
pFN21AB6107
##################################
# NO CUT ENZYMES ( 93 enzymes) #
##################################
AatII AccIII AclI AflII AflIII
Alw44I Aor51HI ApaLI AscI AseI
AsnI AspI BalI BbrPI BcgI
BciVI BclI BfrI BglI BglII
BsaAI BseAI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrGI Bst1107I BstXI BstZ17I
ClaI CpoI DrdI DsaI EagI
EarI EclXI Eco47III Eco52I EcoRV
EcoT22I FbaI FseI HindIII HpaI
Ksp632I KspI MaeII MfeI MluI
MluNI MroI MscI MunI NaeI
NcoI NdeI NgoMIV NotI NruI
NsiI PacI PflFI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SapI SbfI
SfiI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI SspI SwaI Tth111I
XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 59 enzymes) #
###################################
Acc65I 617 GGTACC G^GTACC
AccI 1336 GTCGAC GT^[AC][GT]AC
AcyI 487 GGCGCC G[GA]^CG[CT]C
AhdI 103 GACTTGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1690 CCTGAGG CC^T[ACGT]AGG
Asp700I 90 GAACAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 617 GGTACC G^GTACC
AspEI 103 GACTTGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1016 TGCGCA TGC^GCA
AvrII 306 CCTAGG C^CTAGG
BamHI 18 GGATCC G^GATCC
BbeI 487 GGCGCC GGCGC^C
BbsI 312 GAAGAC GAAGAC(2/6)
BbvCI 1229 CCTCAGC CCTCAGC(-5/-2)
BcnI 607 CCCGG CC^[GC]GG
BlnI 306 CCTAGG C^CTAGG
BlpI 1474 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1474 GCTGAGC GC^T[ACGT]AGC
BpuAI 312 GAAGAC GAAGAC(2/6)
BsaBI 336 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 487 GGCGCC G[GA]^CG[CT]C
BsaI 704 GAGACC GGTCTC(1/5)
BsmAI 704 GAGAC GTCTC(1/5)
BspMI 620 ACCTGC ACCTGC(4/8)
BsrBI 1531 GAGCGG CCGCTC(-3/-3)
BssHII 473 GCGCGC G^CGCGC
BstBI 220 TTCGAA TT^CGAA
BstEII 364 GGTTACC G^GT[ACGT]ACC
BstPI 364 GGTTACC G^GT[ACGT]ACC
Bsu36I 1690 CCTGAGG CC^T[ACGT]AGG
CelII 1474 GCTGAGC GC^T[ACGT]AGC
DraI 1296 TTTAAA TTT^AAA
DraIII 672 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 1534 CGGCCA [CT]^GGCC[GA]
Eam1105I 103 GACTTGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1690 CCTGAGG CC^T[ACGT]AGG
EcoO65I 364 GGTTACC G^GT[ACGT]ACC
EcoRI 1144 GAATTC G^AATTC
FspI 1016 TGCGCA TGC^GCA
Hin1I 487 GGCGCC G[GA]^CG[CT]C
KasI 487 GGCGCC G^GCGCC
KpnI 617 GGTACC GGTAC^C
MamI 336 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 1297 TTAA T^TAA
NarI 487 GGCGCC GG^CGCC
NciI 607 CCCGG CC^[GC]GG
NheI 1258 GCTAGC G^CTAGC
NspV 220 TTCGAA TT^CGAA
PaeR7I 547 CTCGAG C^TCGAG
PpuMI 242 GGGTCCT [GA]G^G[AT]CC[CT]
SalI 1336 GTCGAC G^TCGAC
ScaI 833 AGTACT AGT^ACT
SfoI 487 GGCGCC GGC^GCC
SfuI 220 TTCGAA TT^CGAA
SphI 264 GCATGC GCATG^C
StuI 665 AGGCCT AGG^CCT
Tru9I 1297 TTAA T^TAA
XhoI 547 CTCGAG C^TCGAG
XmnI 90 GAACAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccII 474 CGCG CG^CG
1024 CGCG
AcsI 1144 GAATTC [GA]^AATT[CT]
1286 AAATTC
AgeI 641 ACCGGT A^CCGGT
707 ACCGGT
AlwNI 409 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
1705 CAGTACCTG
ApoI 1144 GAATTC [GA]^AATT[CT]
1286 AAATTC
BfaI 307 CTAG C^TAG
1259 CTAG
BpmI 1395 CTCCAG CTGGAG(16/14)
1236 CTGGAG
BsaWI 641 ACCGGT [AT]^CCGG[AT]
707 ACCGGT
BsrDI 582 GCAATG GCAATG(2/0)
633 GCAATG
BssSI 661 CACGAG CACGAG(-5/-1)
211 CTCGTG
BstAPI 296 GCAACATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
671 GCACCCAGTGC
BstUI 474 CGCG CG^CG
1024 CGCG
BstYI 18 GGATCC [GA]^GATC[CT]
335 AGATCC
HaeII 487 GGCGCC [GA]GCGC^[CT]
1087 AGCGCC
HincII 865 GTCAAC GT[CT]^[GA]AC
1336 GTCGAC
HindII 865 GTCAAC GT[CT]^[GA]AC
1336 GTCGAC
MaeI 307 CTAG C^TAG
1259 CTAG
MflI 18 GGATCC [GA]^GATC[CT]
335 AGATCC
MslI 265 CATGCCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
909 CACCCCAGTG
MvnI 474 CGCG CG^CG
1024 CGCG
NspI 264 GCATGC [GA]CATG^[CT]
1445 ACATGC
PinAI 641 ACCGGT A^CCGGT
707 ACCGGT
PstI 505 CTGCAG CTGCA^G
886 CTGCAG
PvuII 1472 CAGCTG CAG^CTG
1634 CAGCTG
SexAI 1664 ACCAGGT A^CC[AT]GGT
1709 ACCTGGT
SfcI 505 CTGCAG C^T[GA][CT]AG
886 CTGCAG
SmlI 547 CTCGAG C^T[CT][GA]AG
1603 CTCAAG
TfiI 6 GAATC G^A[AT]TC
852 GATTC
XcmI 1244 CCAACCACCACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1397 CCAGATGGACAGTGG
XhoII 18 GGATCC [GA]^GATC[CT]
335 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AciI AfaI AluI AlwI ApaI
AspHI AvaI AvaII BanI BanII
BbvI BmyI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmFI BsoBI
Bsp1286I BsrFI BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI MaeIII
MboI MboII MnlI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
PflMI PleI RsaI SacI Sau3AI
Sau96I ScrFI SfaNI StyI TaqI
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I