(Circular) (Six-base) MAPSORT of: pFN21AB6107.seq Check: 8714 from: 1 to: 6445 pFN21AB6107 6445 bp With 182 enzymes: SgfI * April 15, 2010 09:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4255 278 Size: 53 83 186 936 2719 2468 Fragments arranged by size: 2719 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3342 3788 1937 Size: 1405 446 4594 Fragments arranged by size: 4594 1405 446 Acc65I G'GTAC_C Cuts at: 2622 3766 2622 Size: 1144 5301 AclI AA'CG_TT Cuts at: 1563 4572 4945 1563 Size: 3009 373 3063 Fragments arranged by size: 3063 3009 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2504 3510 4508 5892 6160 1632 Size: 872 1006 998 1384 268 1917 Fragments arranged by size: 1917 1384 1006 998 872 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6248 AflIII A'CryG_T Cuts at: 1203 5344 6321 1203 Size: 4141 977 1327 Fragments arranged by size: 4141 1327 977 AgeI A'CCGG_T Cuts at: 2646 2712 5276 2646 Size: 66 2564 3815 Fragments arranged by size: 3815 2564 66 AhdI GACnn_n'nnGTC Cuts at: 2113 5174 2113 Size: 3061 3384 AleI CACnn'nnGTG Cuts at: 1166 2918 1166 Size: 1752 4693 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6445 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3080 3112 3080 Size: 32 6413 AlwNI CAG_nnn'CTG Cuts at: 2419 3715 4188 5760 2419 Size: 1296 473 1572 3104 Fragments arranged by size: 3104 1572 1296 473 ApaI G_GGCC'C Cuts at: 2080 2613 2753 2080 Size: 533 140 5772 Fragments arranged by size: 5772 533 140 ApaLI G'TGCA_C Cuts at: 4502 5658 4502 Size: 1156 5289 ApoI r'AATT_y Cuts at: 1493 3149 3291 3754 3980 4132 6241 1493 Size: 1656 142 463 226 152 2109 1697 Fragments arranged by size: 2109 1697 1656 463 226 152 142 AseI AT'TA_AT Cuts at: 160 1032 4999 160 Size: 872 3967 1606 Fragments arranged by size: 3967 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6445 AvaI C'yCGr_G Cuts at: 1870 1943 2034 2174 2543 2552 3533 3770 1870 Size: 73 91 140 369 9 981 237 4545 Fragments arranged by size: 4545 981 369 237 140 91 73 9 AvrII C'CTAG_G Cuts at: 2311 2311 Size: 6445 BamHI G'GATC_C Cuts at: 2023 3775 4142 2023 Size: 1752 367 4326 Fragments arranged by size: 4326 1752 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2048 2492 2622 3766 5221 Size: 325 217 627 261 444 130 1144 1455 Cuts at: 5221 618 Size: 1842 Fragments arranged by size: 1842 1455 1144 627 444 325 261 217 130 BanII G_rGCy'C Cuts at: 1391 2020 2080 2295 2553 2613 2697 2753 2889 Size: 629 60 215 258 60 84 56 136 Cuts at: 2889 3764 1391 Size: 875 4072 Fragments arranged by size: 4072 875 629 258 215 136 84 60 60 56 BbeI G_GCGC'C Cuts at: 2496 2496 Size: 6445 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2324 928 Size: 971 425 5049 Fragments arranged by size: 5049 971 425 BbvCI CC'TCA_GC Cuts at: 3235 3235 Size: 6445 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4636 4670 1903 Size: 34 2699 34 3678 Fragments arranged by size: 3678 2699 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4337 5547 4337 Size: 1210 5235 BclI T'GATC_A Cuts at: 1548 1548 Size: 6445 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5056 136 Size: 107 122 71 631 605 3384 1525 Fragments arranged by size: 3384 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6440 6440 Size: 6445 BlpI GC'TnA_GC Cuts at: 3480 3862 3480 Size: 382 6063 Bme1580I G_kGCm'C Cuts at: 1790 2080 2613 2753 4506 5662 1790 Size: 290 533 140 1753 1156 2573 Fragments arranged by size: 2573 1753 1156 533 290 140 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6445 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2254 2674 2890 Size: 416 53 83 399 24 837 420 216 Cuts at: 2890 2912 3213 4176 5134 442 Size: 22 301 963 958 1753 Fragments arranged by size: 1753 963 958 837 420 416 399 301 216 83 53 24 22 BmtI G_CTAG'C Cuts at: 1056 3267 1056 Size: 2211 4234 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2578 2578 Size: 6445 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3262 3385 5105 1239 Size: 2023 123 1720 2579 Fragments arranged by size: 2579 2023 1720 123 Bpu10I CC'TnA_GC Cuts at: 3235 3316 3235 Size: 81 6364 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3593 4570 5435 5733 3593 Size: 977 865 298 4305 Fragments arranged by size: 4305 977 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2703 5108 882 Size: 617 1204 2405 2219 Fragments arranged by size: 2405 2219 1204 617 BsaAI yAC'GTr Cuts at: 493 1204 4236 493 Size: 711 3032 2702 Fragments arranged by size: 3032 2702 711 BsaBI GATnn'nnATC Cuts at: 2345 6439 2345 Size: 4094 2351 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2493 4252 4634 5269 Size: 53 83 186 936 960 1759 382 635 Cuts at: 5269 275 Size: 1451 Fragments arranged by size: 1759 1451 960 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2646 2712 4878 5276 5550 5697 6108 2646 Size: 66 2166 398 274 147 411 2983 Fragments arranged by size: 2983 2166 411 398 274 147 66 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2120 2150 2282 2312 2540 Size: 30 165 30 717 30 132 30 228 Cuts at: 2540 2570 2925 2955 3080 3110 1178 Size: 30 355 30 125 30 4513 Fragments arranged by size: 4513 717 355 228 165 132 125 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2303 2561 2867 2933 3088 1186 Size: 1117 258 306 66 155 4543 Fragments arranged by size: 4543 1117 306 258 155 66 BseYI C'CCAG_C Cuts at: 3641 5648 6144 3641 Size: 2007 496 3942 Fragments arranged by size: 3942 2007 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2659 2671 3534 3657 1163 Size: 573 923 12 863 123 3951 Fragments arranged by size: 3951 923 863 573 123 12 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3895 4656 5684 1540 Size: 275 186 1894 761 1028 2301 Fragments arranged by size: 2301 1894 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2020 2553 2889 3248 4506 4591 5662 2020 Size: 533 336 359 1258 85 1071 2803 Fragments arranged by size: 2803 1258 1071 533 359 336 85 BsmI GAATG_Cn' Cuts at: 3956 4049 3956 Size: 93 6352 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2020 2080 2295 2553 2613 2697 2753 Size: 399 230 60 215 258 60 84 56 Cuts at: 2753 2889 3248 3764 4506 4591 5662 1391 Size: 136 359 516 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 516 399 359 258 230 215 136 85 84 60 60 56 BspEI T'CCGG_A Cuts at: 6108 6108 Size: 6445 BspHI T'CATG_A Cuts at: 4334 6064 4334 Size: 1730 4715 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2634 3800 844 Size: 1790 1166 3489 Fragments arranged by size: 3489 1790 1166 BsrBI CCG'CTC Cuts at: 1394 1823 3538 4332 1394 Size: 429 1715 794 3507 Fragments arranged by size: 3507 1715 794 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2594 2645 4940 5114 65 Size: 1183 1346 51 2295 174 1396 Fragments arranged by size: 2295 1396 1346 1183 174 51 BsrFI r'CCGG_y Cuts at: 1700 1810 2148 2273 2646 2712 2970 5089 5276 Size: 110 338 125 373 66 258 2119 187 Cuts at: 5276 1700 Size: 2869 Fragments arranged by size: 2869 2119 373 338 258 187 125 110 66 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6445 BssHII G'CGCG_C Cuts at: 1925 2478 1925 Size: 553 5892 BssSI C'ACGA_G Cuts at: 2216 2666 4505 5517 2216 Size: 450 1839 1012 3144 Fragments arranged by size: 3144 1839 1012 450 BstAPI GCAn_nnn'nTGC Cuts at: 794 2307 2682 794 Size: 1513 375 4557 Fragments arranged by size: 4557 1513 375 BstBI TT'CG_AA Cuts at: 2226 5249 2226 Size: 3023 3422 BstEII G'GTnAC_C Cuts at: 1184 2369 1184 Size: 1185 5260 BstYI r'GATC_y Cuts at: 1972 2023 2340 3775 4142 4527 4544 5996 6082 Size: 51 317 1435 367 385 17 1452 86 Cuts at: 6082 6094 6440 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1435 385 367 346 317 86 51 17 12 Bsu36I CC'TnA_GG Cuts at: 3696 3696 Size: 6445 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4954 Fragments arranged by size: 4954 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2482 2555 2794 487 Size: 683 56 1256 73 239 4138 Fragments arranged by size: 4138 1256 683 239 73 56 BtsI GCAGTG_nn' Cuts at: 2387 2410 2663 3517 3961 4755 4775 2387 Size: 23 253 854 444 794 20 4057 Fragments arranged by size: 4057 854 794 444 253 23 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6445 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6410 DraI TTT'AAA Cuts at: 3303 3749 3303 Size: 446 5999 DraIII CAC_nnn'GTG Cuts at: 2682 2682 Size: 6445 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5452 809 Size: 507 4136 1802 Fragments arranged by size: 4136 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3539 3892 4781 Size: 54 1425 50 275 18 1709 353 889 Cuts at: 4781 6410 8 Size: 1629 43 Fragments arranged by size: 1709 1629 1425 889 353 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3892 1537 Size: 275 2080 4090 Fragments arranged by size: 4090 2080 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4375 1353 Size: 3022 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3005 5032 5406 5552 387 Size: 1064 138 1416 2027 374 146 1280 Fragments arranged by size: 2027 1416 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2018 2551 2887 2018 Size: 533 336 5576 Fragments arranged by size: 5576 533 336 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2504 3262 3385 3510 4508 5105 5892 Size: 393 872 758 123 125 998 597 787 Cuts at: 5892 6160 1239 Size: 268 1524 Fragments arranged by size: 1524 998 872 787 758 597 393 268 125 123 EcoNI CCTnn'n_nnAGG Cuts at: 2084 2255 3066 2084 Size: 171 811 5463 Fragments arranged by size: 5463 811 171 EcoO109I rG'GnC_Cy Cuts at: 2076 2077 2248 2749 2076 Size: 1 171 501 5772 Fragments arranged by size: 5772 501 171 1 EcoRI G'AATT_C Cuts at: 3149 3754 4132 3149 Size: 605 378 5462 Fragments arranged by size: 5462 605 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2116 2857 3028 2116 Size: 741 171 5533 Fragments arranged by size: 5533 741 171 FspI TGC'GCA Cuts at: 3023 4951 3023 Size: 1928 4517 HaeII r_GCGC'y Cuts at: 1678 2496 3096 5592 1678 Size: 818 600 2496 2531 Fragments arranged by size: 2531 2496 818 600 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2099 2131 2433 2465 2925 Size: 32 135 32 527 32 302 32 460 Cuts at: 2925 2957 3218 3250 3329 3361 3578 3610 5086 Size: 32 261 32 79 32 217 32 1476 Cuts at: 5086 5118 5160 5192 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1476 527 460 302 261 217 135 79 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2872 3343 3789 4035 669 Size: 988 281 934 471 446 246 3079 Fragments arranged by size: 3079 988 934 471 446 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6445 HpaI GTT'AAC Cuts at: 4035 4035 Size: 6445 KasI G'GCGC_C Cuts at: 2492 2492 Size: 6445 KpnI G_GTAC'C Cuts at: 2626 3770 2626 Size: 1144 5301 MfeI C'AATT_G Cuts at: 4044 4044 Size: 6445 MluI A'CGCG_T Cuts at: 6321 6321 Size: 6445 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5559 5743 1305 Size: 4254 184 2007 Fragments arranged by size: 4254 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4623 Fragments arranged by size: 4623 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2274 2918 4403 4762 4921 518 Size: 648 1108 644 1485 359 159 2042 Fragments arranged by size: 2042 1485 1108 648 644 359 159 MspA1I CmG'CkG Cuts at: 1606 2332 3479 3641 3861 4538 5686 5931 1606 Size: 726 1147 162 220 677 1148 245 2120 Fragments arranged by size: 2120 1148 1147 726 677 245 220 162 NarI GG'CG_CC Cuts at: 2493 2493 Size: 6445 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4954 Fragments arranged by size: 4954 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6445 NheI G'CTAG_C Cuts at: 1052 3263 1052 Size: 2211 4234 NotI GC'GGCC_GC Cuts at: 3892 3892 Size: 6445 NspI r_CATG'y Cuts at: 1132 1229 2273 3454 3803 5348 1132 Size: 97 1044 1181 349 1545 2229 Fragments arranged by size: 2229 1545 1181 1044 349 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6445 PciI A'CATG_T Cuts at: 5344 5344 Size: 6445 PflMI CCAn_nnn'nTGG Cuts at: 1108 2244 3259 3676 1108 Size: 1136 1015 417 3877 Fragments arranged by size: 3877 1136 1015 417 PfoI T'CCnGG_A Cuts at: 2281 2281 Size: 6445 PmeI GTTT'AAAC Cuts at: 3749 3749 Size: 6445 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3080 3112 4514 4546 6027 6059 3080 Size: 32 1402 32 1481 32 3466 Fragments arranged by size: 3466 1481 1402 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2248 2248 Size: 6445 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6445 PsiI TTA'TAA Cuts at: 2964 4015 2964 Size: 1051 5394 PspOMI G'GGCC_C Cuts at: 2076 2609 2749 2076 Size: 533 140 5772 Fragments arranged by size: 5772 533 140 PspXI vC'TCGA_Gb Cuts at: 2552 2552 Size: 6445 PstI C_TGCA'G Cuts at: 830 2514 2895 3797 830 Size: 1684 381 902 3478 Fragments arranged by size: 3478 1684 902 381 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6445 PvuII CAG'CTG Cuts at: 3479 3641 3479 Size: 162 6283 SacI G_AGCT'C Cuts at: 2020 2553 2889 2020 Size: 533 336 5576 Fragments arranged by size: 5576 533 336 SalI G'TCGA_C Cuts at: 1936 3341 3787 1936 Size: 1405 446 4594 Fragments arranged by size: 4594 1405 446 SbfI CC_TGCA'GG Cuts at: 3797 3797 Size: 6445 ScaI AGT'ACT Cuts at: 2840 4693 6405 2840 Size: 1853 1712 2880 Fragments arranged by size: 2880 1853 1712 SexAI A'CCwGG_T Cuts at: 3669 3714 3669 Size: 45 6400 SfcI C'TryA_G Cuts at: 826 1045 2510 2891 3793 4928 5609 5800 826 Size: 219 1465 381 902 1135 681 191 1471 Fragments arranged by size: 1471 1465 1135 902 681 381 219 191 SfoI GGC'GCC Cuts at: 2494 2494 Size: 6445 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6445 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6445 SmaI CCC'GGG Cuts at: 1872 3772 1872 Size: 1900 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2552 3608 4549 5450 5712 820 Size: 197 926 609 1056 941 901 262 1553 Fragments arranged by size: 1553 1056 941 926 901 609 262 197 SnaBI TAC'GTA Cuts at: 493 4236 493 Size: 3743 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6445 SphI G_CATG'C Cuts at: 1132 2273 3803 1132 Size: 1141 1530 3774 Fragments arranged by size: 3774 1530 1141 SspI AAT'ATT Cuts at: 5 52 4369 5 Size: 47 4317 2081 Fragments arranged by size: 4317 2081 47 StuI AGG'CCT Cuts at: 1517 2672 1517 Size: 1155 5290 StyI C'CwwG_G Cuts at: 513 1065 2004 2311 2429 3441 3884 513 Size: 552 939 307 118 1012 443 3074 Fragments arranged by size: 3074 1012 939 552 443 307 118 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2693 2773 3052 4472 4489 4642 4827 Size: 1255 1113 80 279 1420 17 153 185 Cuts at: 4827 5272 325 Size: 445 1498 Fragments arranged by size: 1498 1420 1255 1113 445 279 185 153 80 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2166 2757 2838 Size: 274 80 33 51 1443 189 591 81 Cuts at: 2838 4691 6403 96 Size: 1853 1712 138 Fragments arranged by size: 1853 1712 1443 591 274 189 138 81 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2282 2314 2540 2572 6405 6437 2282 Size: 32 226 32 3833 32 2290 Fragments arranged by size: 3833 2290 226 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6445 XbaI T'CTAG_A Cuts at: 3781 3781 Size: 6445 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3256 3409 3256 Size: 153 6292 XhoI C'TCGA_G Cuts at: 1943 2552 1943 Size: 609 5836 XmaI C'CCGG_G Cuts at: 1870 3770 1870 Size: 1900 4545 XmnI GAAnn'nnTTC Cuts at: 2099 4574 2099 Size: 2475 3970 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4253 276 Size: 53 83 186 936 2719 2468 Fragments arranged by size: 2719 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI BaeI BfrBI BsiWI BsmBI BstXI BstZ17I EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PsrI RsrII SacII SanDI SapI SfiI SrfI SwaI