(Circular) (Six-base) MAPSORT of: pFN21AB6102.seq Check: 7024 from: 1 to: 6547 pFN21AB6102 6547 bp With 182 enzymes: SgfI * April 15, 2010 09:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3820 3820 Size: 6547 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4357 278 Size: 53 83 186 936 2821 2468 Fragments arranged by size: 2821 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3890 1937 Size: 1953 4594 Acc65I G'GTAC_C Cuts at: 3868 3868 Size: 6547 AclI AA'CG_TT Cuts at: 1563 4674 5047 1563 Size: 3111 373 3063 Fragments arranged by size: 3111 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2891 4610 5994 6262 1632 Size: 1259 1719 1384 268 1917 Fragments arranged by size: 1917 1719 1384 1259 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6350 AflIII A'CryG_T Cuts at: 1203 5446 6423 1203 Size: 4243 977 1327 Fragments arranged by size: 4243 1327 977 AgeI A'CCGG_T Cuts at: 5378 5378 Size: 6547 AhdI GACnn_n'nnGTC Cuts at: 5276 5276 Size: 6547 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6547 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2862 1184 Size: 1678 4869 AlwNI CAG_nnn'CTG Cuts at: 2644 3594 3673 4290 5862 2644 Size: 950 79 617 1572 3329 Fragments arranged by size: 3329 1572 950 617 79 ApaLI G'TGCA_C Cuts at: 2720 4604 5760 2720 Size: 1884 1156 3507 Fragments arranged by size: 3507 1884 1156 ApoI r'AATT_y Cuts at: 1493 2504 3381 3856 4082 4234 6343 1493 Size: 1011 877 475 226 152 2109 1697 Fragments arranged by size: 2109 1697 1011 877 475 226 152 AseI AT'TA_AT Cuts at: 160 1032 5101 160 Size: 872 4069 1606 Fragments arranged by size: 4069 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6547 AvaI C'yCGr_G Cuts at: 1870 1943 2613 2873 3872 1870 Size: 73 670 260 999 4545 Fragments arranged by size: 4545 999 670 260 73 BamHI G'GATC_C Cuts at: 3877 4244 3877 Size: 367 6180 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2113 2279 3648 3868 5323 Size: 325 217 627 326 166 1369 220 1455 Cuts at: 5323 618 Size: 1842 Fragments arranged by size: 1842 1455 1369 627 326 325 220 217 166 BanII G_rGCy'C Cuts at: 1391 3689 3731 3866 1391 Size: 2298 42 135 4072 Fragments arranged by size: 4072 2298 135 42 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5576 BbvCI CC'TCA_GC Cuts at: 3221 3417 3221 Size: 196 6351 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2594 2628 3777 3811 4738 4772 1903 Size: 34 657 34 1149 34 927 34 3678 Fragments arranged by size: 3678 1149 927 657 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3320 4439 5649 3320 Size: 1119 1210 4218 Fragments arranged by size: 4218 1210 1119 BclI T'GATC_A Cuts at: 1548 1548 Size: 6547 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3693 5158 136 Size: 107 122 71 631 605 2021 1465 1525 Fragments arranged by size: 2021 1525 1465 631 605 122 107 71 BglII A'GATC_T Cuts at: 6542 6542 Size: 6547 BlpI GC'TnA_GC Cuts at: 2649 3964 2649 Size: 1315 5232 Bme1580I G_kGCm'C Cuts at: 1790 2284 2724 2862 3762 4608 5764 1790 Size: 494 440 138 900 846 1156 2573 Fragments arranged by size: 2573 1156 900 846 494 440 138 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6547 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2632 2736 3649 Size: 416 53 83 399 24 1215 104 913 Cuts at: 3649 4278 5236 442 Size: 629 958 1753 Fragments arranged by size: 1753 1215 958 913 629 416 399 104 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6547 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3262 3262 Size: 6547 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2223 3232 5207 1239 Size: 984 1009 1975 2579 Fragments arranged by size: 2579 1975 1009 984 Bpu10I CC'TnA_GC Cuts at: 2793 3221 3417 2793 Size: 428 196 5923 Fragments arranged by size: 5923 428 196 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2552 4672 5537 5835 2552 Size: 2120 865 298 3264 Fragments arranged by size: 3264 2120 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3250 5210 882 Size: 617 1751 1960 2219 Fragments arranged by size: 2219 1960 1751 617 BsaAI yAC'GTr Cuts at: 493 1204 3331 4338 493 Size: 711 2127 1007 2702 Fragments arranged by size: 2702 2127 1007 711 BsaBI GATnn'nnATC Cuts at: 6541 6541 Size: 6547 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4354 4736 5371 275 Size: 53 83 186 936 2821 382 635 1451 Fragments arranged by size: 2821 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4980 5378 5652 5799 6210 4980 Size: 398 274 147 411 5317 Fragments arranged by size: 5317 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2773 2803 1178 Size: 30 165 30 1370 30 4922 Fragments arranged by size: 4922 1370 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2253 2311 2767 2823 3551 3721 1186 Size: 1067 58 456 56 728 170 4012 Fragments arranged by size: 4012 1067 728 456 170 58 56 BseYI C'CCAG_C Cuts at: 2084 3761 3766 5750 6246 2084 Size: 1677 5 1984 496 2385 Fragments arranged by size: 2385 1984 1677 496 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2319 2588 3291 1163 Size: 573 583 269 703 4419 Fragments arranged by size: 4419 703 583 573 269 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3707 3997 4758 5786 1540 Size: 275 186 1706 290 761 1028 2301 Fragments arranged by size: 2301 1706 1028 761 290 275 186 BsiHKAI G_wGCw'C Cuts at: 2374 2724 3463 4608 4693 5764 2374 Size: 350 739 1145 85 1071 3157 Fragments arranged by size: 3157 1145 1071 739 350 85 BsmI GAATG_Cn' Cuts at: 4058 4151 4058 Size: 93 6454 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2284 2374 2724 2862 3463 3689 3731 Size: 399 494 90 350 138 601 226 42 Cuts at: 3731 3762 3866 4608 4693 5764 1391 Size: 31 104 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 601 494 399 350 226 138 104 90 85 42 31 BspEI T'CCGG_A Cuts at: 6210 6210 Size: 6547 BspHI T'CATG_A Cuts at: 2865 4436 6166 2865 Size: 1571 1730 3246 Fragments arranged by size: 3246 1730 1571 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2586 3681 3820 3902 844 Size: 1742 1095 139 82 3489 Fragments arranged by size: 3489 1742 1095 139 82 BsrBI CCG'CTC Cuts at: 1394 1823 3646 4434 1394 Size: 429 1823 788 3507 Fragments arranged by size: 3507 1823 788 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2251 2314 2431 2679 3292 5042 5216 Size: 1183 1003 63 117 248 613 1750 174 Cuts at: 5216 65 Size: 1396 Fragments arranged by size: 1750 1396 1183 1003 613 248 174 117 63 BsrFI r'CCGG_y Cuts at: 1700 1810 3702 5191 5378 1700 Size: 110 1892 1489 187 2869 Fragments arranged by size: 2869 1892 1489 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6547 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6547 BssSI C'ACGA_G Cuts at: 4607 5619 4607 Size: 1012 5535 BstAPI GCAn_nnn'nTGC Cuts at: 794 2690 3509 3594 794 Size: 1896 819 85 3747 Fragments arranged by size: 3747 1896 819 85 BstBI TT'CG_AA Cuts at: 5351 5351 Size: 6547 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6547 BstXI CCAn_nnnn'nTGG Cuts at: 2084 3005 2084 Size: 921 5626 BstYI r'GATC_y Cuts at: 1972 2159 3877 4244 4629 4646 6098 6184 6196 Size: 187 1718 367 385 17 1452 86 12 Cuts at: 6196 6542 1972 Size: 346 1977 Fragments arranged by size: 1977 1718 1452 385 367 346 187 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2050 2050 Size: 6547 BtgI C'CryG_G Cuts at: 513 1065 2004 3543 3691 513 Size: 552 939 1539 148 3369 Fragments arranged by size: 3369 1539 939 552 148 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2135 2670 3780 487 Size: 683 56 909 535 1110 3254 Fragments arranged by size: 3254 1110 909 683 535 56 BtsI GCAGTG_nn' Cuts at: 2605 3595 4063 4857 4877 2605 Size: 990 468 794 20 4275 Fragments arranged by size: 4275 990 794 468 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6547 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6512 DraI TTT'AAA Cuts at: 3851 3851 Size: 6547 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3000 5554 809 Size: 507 1684 2554 1802 Fragments arranged by size: 2554 1802 1684 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2023 2619 3565 Size: 54 1425 50 275 18 193 596 946 Cuts at: 3565 3679 3694 3704 3994 4883 6512 8 Size: 114 15 10 290 889 1629 43 Fragments arranged by size: 1629 1425 946 889 596 290 275 193 114 54 50 43 18 15 10 EagI C'GGCC_G Cuts at: 1537 1812 3704 3994 1537 Size: 275 1892 290 4090 Fragments arranged by size: 4090 1892 290 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2729 2754 2910 4477 1353 Size: 1376 25 156 1567 3423 Fragments arranged by size: 3423 1567 1376 156 25 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3733 5134 5508 5654 387 Size: 1064 138 2144 1401 374 146 1280 Fragments arranged by size: 2144 1401 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2223 2891 3232 4610 5207 5994 6262 Size: 393 591 668 341 1378 597 787 268 Cuts at: 6262 1239 Size: 1524 Fragments arranged by size: 1524 1378 787 668 597 591 393 341 268 EcoNI CCTnn'n_nnAGG Cuts at: 3736 3736 Size: 6547 EcoRI G'AATT_C Cuts at: 2504 3856 4234 2504 Size: 1352 378 4817 Fragments arranged by size: 4817 1352 378 FseI GG_CCGG'CC Cuts at: 3706 3706 Size: 6547 FspI TGC'GCA Cuts at: 5053 5053 Size: 6547 HaeII r_GCGC'y Cuts at: 1678 3136 3157 5694 1678 Size: 1458 21 2537 2531 Fragments arranged by size: 2537 2531 1458 21 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2728 2760 3767 3799 5188 Size: 32 135 32 1156 32 1007 32 1389 Cuts at: 5188 5220 5262 5294 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1389 1156 1007 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3891 4137 669 Size: 988 281 1953 246 3079 Fragments arranged by size: 3079 1953 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6547 HpaI GTT'AAC Cuts at: 4137 4137 Size: 6547 KpnI G_GTAC'C Cuts at: 3872 3872 Size: 6547 MfeI C'AATT_G Cuts at: 2351 2496 2784 4146 2351 Size: 145 288 1362 4752 Fragments arranged by size: 4752 1362 288 145 MluI A'CGCG_T Cuts at: 6423 6423 Size: 6547 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5661 5845 1305 Size: 4356 184 2007 Fragments arranged by size: 4356 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2025 3681 10 Size: 54 1425 343 193 1656 2876 Fragments arranged by size: 2876 1656 1425 343 193 54 MslI CAynn'nnrTG Cuts at: 518 1166 2717 2727 4505 4864 5023 518 Size: 648 1551 10 1778 359 159 2042 Fragments arranged by size: 2042 1778 1551 648 359 159 10 MspA1I CmG'CkG Cuts at: 1606 2593 3709 3963 4640 5788 6033 1606 Size: 987 1116 254 677 1148 245 2120 Fragments arranged by size: 2120 1148 1116 987 677 254 245 NaeI GCC'GGC Cuts at: 3704 3704 Size: 6547 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5056 Fragments arranged by size: 5056 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6547 NgoMIV G'CCGG_C Cuts at: 3702 3702 Size: 6547 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6547 NotI GC'GGCC_GC Cuts at: 3994 3994 Size: 6547 NspI r_CATG'y Cuts at: 1132 1229 3588 3905 5450 1132 Size: 97 2359 317 1545 2229 Fragments arranged by size: 2359 2229 1545 317 97 PasI CC'CwG_GG Cuts at: 1348 3263 1348 Size: 1915 4632 PciI A'CATG_T Cuts at: 5446 5446 Size: 6547 PflMI CCAn_nnn'nTGG Cuts at: 1108 2644 1108 Size: 1536 5011 PfoI T'CCnGG_A Cuts at: 2200 2200 Size: 6547 PmeI GTTT'AAAC Cuts at: 3851 3851 Size: 6547 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4616 4648 6129 6161 4616 Size: 32 1481 32 5002 Fragments arranged by size: 5002 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6547 PsiI TTA'TAA Cuts at: 4117 4117 Size: 6547 PstI C_TGCA'G Cuts at: 830 2592 3755 3899 830 Size: 1762 1163 144 3478 Fragments arranged by size: 3478 1762 1163 144 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6547 SalI G'TCGA_C Cuts at: 1936 3889 1936 Size: 1953 4594 SbfI CC_TGCA'GG Cuts at: 3899 3899 Size: 6547 ScaI AGT'ACT Cuts at: 4795 6507 4795 Size: 1712 4835 SfcI C'TryA_G Cuts at: 826 1045 2588 3717 3751 3895 5030 5711 5902 Size: 219 1543 1129 34 144 1135 681 191 Cuts at: 5902 826 Size: 1471 Fragments arranged by size: 1543 1471 1135 1129 681 219 191 144 34 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6547 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6547 SmaI CCC'GGG Cuts at: 1872 2875 3874 1872 Size: 1003 999 4545 Fragments arranged by size: 4545 1003 999 SmlI C'TyrA_G Cuts at: 820 1017 1943 2567 4651 5552 5814 820 Size: 197 926 624 2084 901 262 1553 Fragments arranged by size: 2084 1553 926 901 624 262 197 SnaBI TAC'GTA Cuts at: 493 3331 4338 493 Size: 2838 1007 2702 Fragments arranged by size: 2838 2702 1007 SpeI A'CTAG_T Cuts at: 152 152 Size: 6547 SphI G_CATG'C Cuts at: 1132 3905 1132 Size: 2773 3774 SspI AAT'ATT Cuts at: 5 52 4471 5 Size: 47 4419 2081 Fragments arranged by size: 4419 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6547 StyI C'CwwG_G Cuts at: 513 1065 2004 3986 513 Size: 552 939 1982 3074 Fragments arranged by size: 3074 1982 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2224 3251 4574 4591 4744 4929 5374 Size: 1255 644 1027 1323 17 153 185 445 Cuts at: 5374 325 Size: 1498 Fragments arranged by size: 1498 1323 1255 1027 644 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3400 4793 6505 Size: 274 80 33 51 1443 1423 1393 1712 Cuts at: 6505 96 Size: 138 Fragments arranged by size: 1712 1443 1423 1393 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2121 2153 2521 2553 2773 2805 6507 6539 2121 Size: 32 368 32 220 32 3702 32 2129 Fragments arranged by size: 3702 2129 368 220 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6547 XbaI T'CTAG_A Cuts at: 3883 3883 Size: 6547 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3659 3659 Size: 6547 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6547 XmaI C'CCGG_G Cuts at: 1870 2873 3872 1870 Size: 1003 999 4545 Fragments arranged by size: 4545 1003 999 XmnI GAAnn'nnTTC Cuts at: 2661 4676 2661 Size: 2015 4532 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4355 276 Size: 53 83 186 936 2821 2468 Fragments arranged by size: 2821 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoO109I EcoRV FalI FspAI KasI NarI NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI