(Circular) (Six-base) MAPSORT of: pFN21AB6101.seq Check: 6939 from: 1 to: 6436 pFN21AB6101 6436 bp With 182 enzymes: SgfI * April 15, 2010 09:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2859 2877 4246 278 Size: 53 83 186 936 1323 18 1369 2468 Fragments arranged by size: 2468 1369 1323 936 186 83 53 18 AccI GT'mk_AC Cuts at: 1937 2836 3051 3779 1937 Size: 899 215 728 4594 Fragments arranged by size: 4594 899 728 215 Acc65I G'GTAC_C Cuts at: 3757 3757 Size: 6436 AclI AA'CG_TT Cuts at: 1563 2092 4563 4936 1563 Size: 529 2471 373 3063 Fragments arranged by size: 3063 2471 529 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3338 4499 5883 6151 1632 Size: 1706 1161 1384 268 1917 Fragments arranged by size: 1917 1706 1384 1161 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6239 AflIII A'CryG_T Cuts at: 1203 3177 5335 6312 1203 Size: 1974 2158 977 1327 Fragments arranged by size: 2158 1974 1327 977 AgeI A'CCGG_T Cuts at: 5267 5267 Size: 6436 AhdI GACnn_n'nnGTC Cuts at: 5165 5165 Size: 6436 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6436 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2499 1184 Size: 1315 5121 AlwNI CAG_nnn'CTG Cuts at: 3122 4179 5751 3122 Size: 1057 1572 3807 Fragments arranged by size: 3807 1572 1057 ApaI G_GGCC'C Cuts at: 3405 3405 Size: 6436 ApaLI G'TGCA_C Cuts at: 4493 5649 4493 Size: 1156 5280 ApoI r'AATT_y Cuts at: 1493 3745 3971 4123 6232 1493 Size: 2252 226 152 2109 1697 Fragments arranged by size: 2252 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4990 160 Size: 872 3958 1606 Fragments arranged by size: 3958 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6436 AvaI C'yCGr_G Cuts at: 1870 1943 2860 3672 3761 1870 Size: 73 917 812 89 4545 Fragments arranged by size: 4545 917 812 89 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3166 3199 3166 Size: 33 6403 BamHI G'GATC_C Cuts at: 3265 3572 3766 4133 3265 Size: 307 194 367 5568 Fragments arranged by size: 5568 367 307 194 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2021 3689 3757 5212 618 Size: 325 217 627 234 1668 68 1455 1842 Fragments arranged by size: 1842 1668 1455 627 325 234 217 68 BanII G_rGCy'C Cuts at: 1391 2016 2810 3395 3405 3755 1391 Size: 625 794 585 10 350 4072 Fragments arranged by size: 4072 794 625 585 350 10 BbeI G_GCGC'C Cuts at: 2025 2025 Size: 6436 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5465 BbvCI CC'TCA_GC Cuts at: 2539 2539 Size: 6436 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2356 2390 3276 3310 3429 3463 3474 Size: 34 419 34 886 34 119 34 11 Cuts at: 3474 3508 4627 4661 1903 Size: 34 1119 34 3678 Fragments arranged by size: 3678 1119 886 419 119 34 34 34 34 34 34 11 BciVI GTATCCnnnnn_n' Cuts at: 3194 4328 5538 3194 Size: 1134 1210 4092 Fragments arranged by size: 4092 1210 1134 BclI T'GATC_A Cuts at: 1548 1548 Size: 6436 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2486 5047 136 Size: 107 122 71 631 605 814 2561 1525 Fragments arranged by size: 2561 1525 814 631 605 122 107 71 BglII A'GATC_T Cuts at: 3315 3372 6431 3315 Size: 57 3059 3320 Fragments arranged by size: 3320 3059 57 BlpI GC'TnA_GC Cuts at: 2469 3853 2469 Size: 1384 5052 Bme1580I G_kGCm'C Cuts at: 1790 3405 4497 5653 1790 Size: 1615 1092 1156 2573 Fragments arranged by size: 2573 1615 1156 1092 BmgBI CAC'GTC Cuts at: 1318 2428 1318 Size: 1110 5326 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3209 3408 4167 Size: 416 53 83 399 24 1792 199 759 Cuts at: 4167 5125 442 Size: 958 1753 Fragments arranged by size: 1792 1753 958 759 416 399 199 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2747 1056 Size: 1691 4745 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2480 2480 Size: 6436 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2189 2300 2778 3340 5096 1239 Size: 950 111 478 562 1756 2579 Fragments arranged by size: 2579 1756 950 562 478 111 Bpu10I CC'TnA_GC Cuts at: 2539 2539 Size: 6436 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4561 5426 5724 4561 Size: 865 298 5273 Fragments arranged by size: 5273 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5099 882 Size: 617 3600 2219 Fragments arranged by size: 3600 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4227 493 Size: 711 3023 2702 Fragments arranged by size: 3023 2702 711 BsaBI GATnn'nnATC Cuts at: 2351 6430 2351 Size: 4079 2357 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2022 2412 2856 2874 Size: 53 83 186 936 489 390 444 18 Cuts at: 2874 3489 4243 4625 5260 275 Size: 615 754 382 635 1451 Fragments arranged by size: 1451 936 754 635 615 489 444 390 382 186 83 53 18 BsaWI w'CCGG_w Cuts at: 2750 4869 5267 5541 5688 6099 2750 Size: 2119 398 274 147 411 3087 Fragments arranged by size: 3087 2119 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2095 2125 2792 2822 3407 Size: 30 165 30 692 30 667 30 585 Cuts at: 3407 3437 3669 3699 1178 Size: 30 232 30 3915 Fragments arranged by size: 3915 692 667 585 232 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3690 1186 Size: 2504 3932 BseYI C'CCAG_C Cuts at: 2814 5639 6135 2814 Size: 2825 496 3115 Fragments arranged by size: 3115 2825 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2453 3214 3478 1163 Size: 573 717 761 264 4121 Fragments arranged by size: 4121 761 717 573 264 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2298 2383 3886 4647 5675 1540 Size: 275 186 297 85 1503 761 1028 2301 Fragments arranged by size: 2301 1503 1028 761 297 275 186 85 BsiHKAI G_wGCw'C Cuts at: 2016 2611 4497 4582 5653 2016 Size: 595 1886 85 1071 2799 Fragments arranged by size: 2799 1886 1071 595 85 BsmI GAATG_Cn' Cuts at: 2450 3947 4040 2450 Size: 1497 93 4846 Fragments arranged by size: 4846 1497 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2016 2611 2810 3395 3405 3755 4497 Size: 399 226 595 199 585 10 350 742 Cuts at: 4497 4582 5653 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 595 585 399 350 226 199 85 10 BspEI T'CCGG_A Cuts at: 2750 6099 2750 Size: 3349 3087 BspHI T'CATG_A Cuts at: 4325 6055 4325 Size: 1730 4706 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3791 844 Size: 2947 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4323 1394 Size: 429 2500 3507 Fragments arranged by size: 3507 2500 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4931 5105 65 Size: 1183 3683 174 1396 Fragments arranged by size: 3683 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2293 2543 5080 5267 1700 Size: 110 483 250 2537 187 2869 Fragments arranged by size: 2869 2537 483 250 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6436 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6436 BssSI C'ACGA_G Cuts at: 2819 4496 5508 2819 Size: 1677 1012 3747 Fragments arranged by size: 3747 1677 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6436 BstBI TT'CG_AA Cuts at: 5240 5240 Size: 6436 BstEII G'GTnAC_C Cuts at: 1184 2960 1184 Size: 1776 4660 BstYI r'GATC_y Cuts at: 1972 2753 3265 3315 3372 3572 3724 3766 4133 Size: 781 512 50 57 200 152 42 367 Cuts at: 4133 4518 4535 5987 6073 6085 6431 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 781 512 385 367 346 200 152 86 57 50 42 17 12 BstZ17I GTA'TAC Cuts at: 2837 2837 Size: 6436 Bsu36I CC'TnA_GG Cuts at: 2977 2977 Size: 6436 BtgI C'CryG_G Cuts at: 513 1065 2508 2583 513 Size: 552 1443 75 4366 Fragments arranged by size: 4366 1443 552 75 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2121 487 Size: 683 56 895 4802 Fragments arranged by size: 4802 895 683 56 BtsI GCAGTG_nn' Cuts at: 3952 4746 4766 3952 Size: 794 20 5622 Fragments arranged by size: 5622 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6436 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6401 DraI TTT'AAA Cuts at: 3740 3740 Size: 6436 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3215 3587 5443 809 Size: 507 1899 372 1856 1802 Fragments arranged by size: 1899 1856 1802 507 372 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2295 2380 2505 Size: 54 1425 50 275 18 465 85 125 Cuts at: 2505 2967 3564 3883 4772 6401 8 Size: 462 597 319 889 1629 43 Fragments arranged by size: 1629 1425 889 597 465 462 319 275 125 85 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2295 2380 3883 1537 Size: 275 483 85 1503 4090 Fragments arranged by size: 4090 1503 483 275 85 EarI CTCTTCn'nnn_ Cuts at: 1353 2032 2050 2592 4366 1353 Size: 679 18 542 1774 3423 Fragments arranged by size: 3423 1774 679 542 18 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5023 5397 5543 387 Size: 1064 138 3434 374 146 1280 Fragments arranged by size: 3434 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2014 2014 Size: 6436 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2189 2300 2778 3338 3340 4499 5096 Size: 393 557 111 478 560 2 1159 597 Cuts at: 5096 5883 6151 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1159 787 597 560 557 478 393 268 111 2 EcoO109I rG'GnC_Cy Cuts at: 2323 2993 2323 Size: 670 5766 EcoRI G'AATT_C Cuts at: 3745 4123 3745 Size: 378 6058 FspI TGC'GCA Cuts at: 4942 4942 Size: 6436 HaeII r_GCGC'y Cuts at: 1678 2025 5583 1678 Size: 347 3558 2531 Fragments arranged by size: 3558 2531 347 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2384 2416 2651 2683 2864 Size: 32 135 32 812 32 235 32 181 Cuts at: 2864 2896 3601 3633 5077 5109 5151 5183 1373 Size: 32 705 32 1444 32 42 32 2626 Fragments arranged by size: 2626 1444 812 705 235 181 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3250 3780 4026 669 Size: 988 281 1312 530 246 3079 Fragments arranged by size: 3079 1312 988 530 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6436 HpaI GTT'AAC Cuts at: 4026 4026 Size: 6436 KasI G'GCGC_C Cuts at: 2021 2021 Size: 6436 KpnI G_GTAC'C Cuts at: 3761 3761 Size: 6436 MfeI C'AATT_G Cuts at: 4035 4035 Size: 6436 MluI A'CGCG_T Cuts at: 6312 6312 Size: 6436 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2292 3450 3726 5550 5734 1305 Size: 987 1158 276 1824 184 2007 Fragments arranged by size: 2007 1824 1158 987 276 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2969 3566 10 Size: 54 1425 343 1137 597 2880 Fragments arranged by size: 2880 1425 1137 597 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3176 4394 4753 4912 518 Size: 648 2010 1218 359 159 2042 Fragments arranged by size: 2042 2010 1218 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3852 4529 5677 5922 1606 Size: 2246 677 1148 245 2120 Fragments arranged by size: 2246 2120 1148 677 245 NaeI GCC'GGC Cuts at: 2545 2545 Size: 6436 NarI GG'CG_CC Cuts at: 2022 2022 Size: 6436 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5884 NdeI CA'TA_TG Cuts at: 387 2926 387 Size: 2539 3897 NgoMIV G'CCGG_C Cuts at: 2543 2543 Size: 6436 NheI G'CTAG_C Cuts at: 1052 2743 1052 Size: 1691 4745 NotI GC'GGCC_GC Cuts at: 3883 3883 Size: 6436 NspI r_CATG'y Cuts at: 1132 1229 3181 3794 5339 1132 Size: 97 1952 613 1545 2229 Fragments arranged by size: 2229 1952 1545 613 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6436 PciI A'CATG_T Cuts at: 3177 5335 3177 Size: 2158 4278 PflMI CCAn_nnn'nTGG Cuts at: 1108 3261 3387 1108 Size: 2153 126 4157 Fragments arranged by size: 4157 2153 126 PfoI T'CCnGG_A Cuts at: 2205 2205 Size: 6436 PmeI GTTT'AAAC Cuts at: 3740 3740 Size: 6436 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4505 4537 6018 6050 4505 Size: 32 1481 32 4891 Fragments arranged by size: 4891 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6436 PsiI TTA'TAA Cuts at: 4006 4006 Size: 6436 PspOMI G'GGCC_C Cuts at: 3401 3401 Size: 6436 PstI C_TGCA'G Cuts at: 830 2053 2331 3788 830 Size: 1223 278 1457 3478 Fragments arranged by size: 3478 1457 1223 278 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6436 SacI G_AGCT'C Cuts at: 2016 2016 Size: 6436 SalI G'TCGA_C Cuts at: 1936 3778 1936 Size: 1842 4594 SbfI CC_TGCA'GG Cuts at: 2053 3788 2053 Size: 1735 4701 ScaI AGT'ACT Cuts at: 3560 4684 6396 3560 Size: 1124 1712 3600 Fragments arranged by size: 3600 1712 1124 SfcI C'TryA_G Cuts at: 826 1045 2049 2327 3166 3529 3784 4919 5600 Size: 219 1004 278 839 363 255 1135 681 Cuts at: 5600 5791 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1004 839 681 363 278 255 219 191 SfoI GGC'GCC Cuts at: 2023 2023 Size: 6436 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6436 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6436 SmaI CCC'GGG Cuts at: 1872 3763 1872 Size: 1891 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4540 5441 5703 820 Size: 197 926 2597 901 262 1553 Fragments arranged by size: 2597 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4227 493 Size: 3734 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6436 SphI G_CATG'C Cuts at: 1132 3794 1132 Size: 2662 3774 SspI AAT'ATT Cuts at: 5 52 4360 5 Size: 47 4308 2081 Fragments arranged by size: 4308 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6436 StyI C'CwwG_G Cuts at: 513 1065 3875 513 Size: 552 2810 3074 Fragments arranged by size: 3074 2810 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2024 2828 3512 3677 4463 4480 4633 Size: 1255 444 804 684 165 786 17 153 Cuts at: 4633 4818 5263 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 804 786 684 445 444 185 165 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3558 4682 6394 Size: 274 80 33 51 1443 1581 1124 1712 Cuts at: 6394 96 Size: 138 Fragments arranged by size: 1712 1581 1443 1124 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2095 2127 3669 3701 6396 6428 2095 Size: 32 1542 32 2695 32 2103 Fragments arranged by size: 2695 2103 1542 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6436 XbaI T'CTAG_A Cuts at: 3772 3772 Size: 6436 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3433 3433 Size: 6436 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6436 XmaI C'CCGG_G Cuts at: 1870 3761 1870 Size: 1891 4545 XmnI GAAnn'nnTTC Cuts at: 4565 4565 Size: 6436 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2857 2875 4244 276 Size: 53 83 186 936 1323 18 1369 2468 Fragments arranged by size: 2468 1369 1323 936 186 83 53 18 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BfrBI BsiWI BsmBI BstXI DraIII EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PpuMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI