(Circular) (Six-base) MAPSORT of: pFN21AB5990.seq Check: 2969 from: 1 to: 6532 pFN21AB5990 6532 bp With 182 enzymes: SgfI * April 15, 2010 09:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2016 3215 2016 Size: 1199 5333 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2746 4342 278 Size: 53 83 186 936 1210 1596 2468 Fragments arranged by size: 2468 1596 1210 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2384 3875 1937 Size: 447 1491 4594 Fragments arranged by size: 4594 1491 447 Acc65I G'GTAC_C Cuts at: 3853 3853 Size: 6532 AclI AA'CG_TT Cuts at: 1563 2676 3439 4659 5032 1563 Size: 1113 763 1220 373 3063 Fragments arranged by size: 3063 1220 1113 763 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4595 5979 6247 1632 Size: 2963 1384 268 1917 Fragments arranged by size: 2963 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6335 AflIII A'CryG_T Cuts at: 1203 5431 6408 1203 Size: 4228 977 1327 Fragments arranged by size: 4228 1327 977 AgeI A'CCGG_T Cuts at: 5363 5363 Size: 6532 AhdI GACnn_n'nnGTC Cuts at: 5261 5261 Size: 6532 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6532 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6532 AlwNI CAG_nnn'CTG Cuts at: 2764 4275 5847 2764 Size: 1511 1572 3449 Fragments arranged by size: 3449 1572 1511 ApaLI G'TGCA_C Cuts at: 4589 5745 4589 Size: 1156 5376 ApoI r'AATT_y Cuts at: 1493 2480 3841 4067 4219 6328 1493 Size: 987 1361 226 152 2109 1697 Fragments arranged by size: 2109 1697 1361 987 226 152 AseI AT'TA_AT Cuts at: 160 1032 5086 160 Size: 872 4054 1606 Fragments arranged by size: 4054 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6532 AvaI C'yCGr_G Cuts at: 1870 1943 3327 3857 1870 Size: 73 1384 530 4545 Fragments arranged by size: 4545 1384 530 73 AvrII C'CTAG_G Cuts at: 3502 3502 Size: 6532 BamHI G'GATC_C Cuts at: 3128 3862 4229 3128 Size: 734 367 5431 Fragments arranged by size: 5431 734 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2228 3853 5308 618 Size: 325 217 627 441 1625 1455 1842 Fragments arranged by size: 1842 1625 1455 627 441 325 217 BanII G_rGCy'C Cuts at: 1391 3851 1391 Size: 2460 4072 BbeI G_GCGC'C Cuts at: 2232 2232 Size: 6532 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3358 3716 928 Size: 971 1459 358 3744 Fragments arranged by size: 3744 1459 971 358 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2756 2790 4723 4757 1903 Size: 34 819 34 1933 34 3678 Fragments arranged by size: 3678 1933 819 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4424 5634 4424 Size: 1210 5322 BclI T'GATC_A Cuts at: 1548 1548 Size: 6532 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5143 136 Size: 107 122 71 631 605 3471 1525 Fragments arranged by size: 3471 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6527 6527 Size: 6532 BlpI GC'TnA_GC Cuts at: 3566 3949 3566 Size: 383 6149 Bme1580I G_kGCm'C Cuts at: 1790 4593 5749 1790 Size: 2803 1156 2573 Fragments arranged by size: 2803 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6532 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4263 5221 442 Size: 416 53 83 399 24 2846 958 1753 Fragments arranged by size: 2846 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6532 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2432 3594 5192 1239 Size: 1193 1162 1598 2579 Fragments arranged by size: 2579 1598 1193 1162 Bpu10I CC'TnA_GC Cuts at: 2561 2561 Size: 6532 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4657 5522 5820 4657 Size: 865 298 5369 Fragments arranged by size: 5369 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2082 2923 3612 5195 882 Size: 617 583 841 689 1583 2219 Fragments arranged by size: 2219 1583 841 689 617 583 BsaAI yAC'GTr Cuts at: 493 1204 4323 493 Size: 711 3119 2702 Fragments arranged by size: 3119 2702 711 BsaBI GATnn'nnATC Cuts at: 6526 6526 Size: 6532 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2229 2743 4339 4721 Size: 53 83 186 936 696 514 1596 382 Cuts at: 4721 5356 275 Size: 635 1451 Fragments arranged by size: 1596 1451 936 696 635 514 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4965 5363 5637 5784 6195 4965 Size: 398 274 147 411 5302 Fragments arranged by size: 5302 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3066 3096 3305 3335 1178 Size: 30 165 30 1663 30 209 30 4375 Fragments arranged by size: 4375 1663 209 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2435 3411 1186 Size: 1249 976 4307 Fragments arranged by size: 4307 1249 976 BseYI C'CCAG_C Cuts at: 5735 6231 5735 Size: 496 6036 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2062 3004 3531 1163 Size: 573 326 942 527 4164 Fragments arranged by size: 4164 942 573 527 326 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3982 4743 5771 1540 Size: 275 186 1981 761 1028 2301 Fragments arranged by size: 2301 1981 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4593 4678 5749 4593 Size: 85 1071 5376 Fragments arranged by size: 5376 1071 85 BsmI GAATG_Cn' Cuts at: 2520 2855 4043 4136 2520 Size: 335 1188 93 4916 Fragments arranged by size: 4916 1188 335 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3851 4593 4678 5749 1391 Size: 399 2061 742 85 1071 2174 Fragments arranged by size: 2174 2061 1071 742 399 85 BspEI T'CCGG_A Cuts at: 6195 6195 Size: 6532 BspHI T'CATG_A Cuts at: 4421 6151 4421 Size: 1730 4802 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2016 2131 3215 3887 844 Size: 1172 115 1084 672 3489 Fragments arranged by size: 3489 1172 1084 672 115 BsrBI CCG'CTC Cuts at: 1394 1823 4419 1394 Size: 429 2596 3507 Fragments arranged by size: 3507 2596 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2419 5027 5201 65 Size: 1183 1171 2608 174 1396 Fragments arranged by size: 2608 1396 1183 1171 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5176 5363 1700 Size: 110 3366 187 2869 Fragments arranged by size: 3366 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6532 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6532 BssSI C'ACGA_G Cuts at: 4592 5604 4592 Size: 1012 5520 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6532 BstBI TT'CG_AA Cuts at: 5336 5336 Size: 6532 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6532 BstXI CCAn_nnnn'nTGG Cuts at: 2803 2803 Size: 6532 BstYI r'GATC_y Cuts at: 1972 2500 3128 3862 4229 4614 4631 6083 6169 Size: 528 628 734 367 385 17 1452 86 Cuts at: 6169 6181 6527 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 734 628 528 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2385 2385 Size: 6532 Bsu36I CC'TnA_GG Cuts at: 3450 3450 Size: 6532 BtgI C'CryG_G Cuts at: 513 1065 2004 2933 513 Size: 552 939 929 4112 Fragments arranged by size: 4112 939 929 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5793 Fragments arranged by size: 5793 683 56 BtsI GCAGTG_nn' Cuts at: 2689 2712 2992 3190 3577 3660 4048 4842 4862 Size: 23 280 198 387 83 388 794 20 Cuts at: 4862 2689 Size: 4359 Fragments arranged by size: 4359 794 388 387 280 198 83 23 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6532 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6497 DraI TTT'AAA Cuts at: 3836 3836 Size: 6532 DraIII CAC_nnn'GTG Cuts at: 3815 3815 Size: 6532 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5539 809 Size: 507 4223 1802 Fragments arranged by size: 4223 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3262 3618 3979 Size: 54 1425 50 275 18 1432 356 361 Cuts at: 3979 4868 6497 8 Size: 889 1629 43 Fragments arranged by size: 1629 1432 1425 889 361 356 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3979 1537 Size: 275 2167 4090 Fragments arranged by size: 4090 2167 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2400 2430 2610 4462 1353 Size: 1047 30 180 1852 3423 Fragments arranged by size: 3423 1852 1047 180 30 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5119 5493 5639 387 Size: 1064 138 3530 374 146 1280 Fragments arranged by size: 3530 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2432 3594 4595 5192 5979 6247 1239 Size: 393 800 1162 1001 597 787 268 1524 Fragments arranged by size: 1524 1162 1001 800 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 3486 3486 Size: 6532 EcoRI G'AATT_C Cuts at: 2480 3841 4219 2480 Size: 1361 378 4793 Fragments arranged by size: 4793 1361 378 FspI TGC'GCA Cuts at: 2024 5038 2024 Size: 3014 3518 HaeII r_GCGC'y Cuts at: 1678 2232 5679 1678 Size: 554 3447 2531 Fragments arranged by size: 3447 2531 554 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2642 2674 3066 3098 3116 Size: 32 135 32 1070 32 392 32 18 Cuts at: 3116 3148 3260 3289 3292 3321 3401 3406 3433 Size: 32 112 29 3 29 80 5 27 Cuts at: 3433 3438 5173 5205 5247 5279 1373 Size: 5 1735 32 42 32 2626 Fragments arranged by size: 2626 1735 1070 392 135 112 80 42 32 32 32 32 32 32 32 29 29 27 18 5 5 3 HincII GTy'rAC Cuts at: 669 1657 1938 3876 4122 669 Size: 988 281 1938 246 3079 Fragments arranged by size: 3079 1938 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6532 HpaI GTT'AAC Cuts at: 4122 4122 Size: 6532 KasI G'GCGC_C Cuts at: 2228 2228 Size: 6532 KpnI G_GTAC'C Cuts at: 3857 3857 Size: 6532 MfeI C'AATT_G Cuts at: 4131 4131 Size: 6532 MluI A'CGCG_T Cuts at: 6408 6408 Size: 6532 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5646 5830 1305 Size: 4341 184 2007 Fragments arranged by size: 4341 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3264 3620 10 Size: 54 1425 343 1432 356 2922 Fragments arranged by size: 2922 1432 1425 356 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2545 4490 4849 5008 518 Size: 648 1379 1945 359 159 2042 Fragments arranged by size: 2042 1945 1379 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2076 2435 2452 3948 4625 5773 6018 1606 Size: 470 359 17 1496 677 1148 245 2120 Fragments arranged by size: 2120 1496 1148 677 470 359 245 17 NarI GG'CG_CC Cuts at: 2229 2229 Size: 6532 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5041 Fragments arranged by size: 5041 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6532 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6532 NotI GC'GGCC_GC Cuts at: 3979 3979 Size: 6532 NspI r_CATG'y Cuts at: 1132 1229 2134 2774 3890 5435 1132 Size: 97 905 640 1116 1545 2229 Fragments arranged by size: 2229 1545 1116 905 640 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6532 PciI A'CATG_T Cuts at: 5431 5431 Size: 6532 PflMI CCAn_nnn'nTGG Cuts at: 1108 3036 3603 3744 1108 Size: 1928 567 141 3896 Fragments arranged by size: 3896 1928 567 141 PmeI GTTT'AAAC Cuts at: 3836 3836 Size: 6532 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4601 4633 6114 6146 4601 Size: 32 1481 32 4987 Fragments arranged by size: 4987 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3486 3486 Size: 6532 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6532 PsiI TTA'TAA Cuts at: 4102 4102 Size: 6532 PstI C_TGCA'G Cuts at: 830 2769 3212 3884 830 Size: 1939 443 672 3478 Fragments arranged by size: 3478 1939 672 443 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6532 PvuII CAG'CTG Cuts at: 2435 2452 2435 Size: 17 6515 SalI G'TCGA_C Cuts at: 1936 3874 1936 Size: 1938 4594 SanDI GG'GwC_CC Cuts at: 3486 3486 Size: 6532 SbfI CC_TGCA'GG Cuts at: 3212 3884 3212 Size: 672 5860 ScaI AGT'ACT Cuts at: 2961 3234 4780 6492 2961 Size: 273 1546 1712 3001 Fragments arranged by size: 3001 1712 1546 273 SfcI C'TryA_G Cuts at: 826 1045 2269 2765 3208 3396 3471 3537 3558 Size: 219 1224 496 443 188 75 66 21 Cuts at: 3558 3880 5015 5696 5887 826 Size: 322 1135 681 191 1471 Fragments arranged by size: 1471 1224 1135 681 496 443 322 219 191 188 75 66 21 SfoI GGC'GCC Cuts at: 2230 2230 Size: 6532 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6532 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6532 SmaI CCC'GGG Cuts at: 1872 3859 1872 Size: 1987 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4636 5537 5799 820 Size: 197 926 2693 901 262 1553 Fragments arranged by size: 2693 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4323 493 Size: 3830 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6532 SphI G_CATG'C Cuts at: 1132 2134 3890 1132 Size: 1002 1756 3774 Fragments arranged by size: 3774 1756 1002 SspI AAT'ATT Cuts at: 5 52 4456 5 Size: 47 4404 2081 Fragments arranged by size: 4404 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6532 StyI C'CwwG_G Cuts at: 513 1065 2004 2083 2166 2921 3502 3971 513 Size: 552 939 79 83 755 581 469 3074 Fragments arranged by size: 3074 939 755 581 552 469 83 79 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2597 3248 4559 4576 4729 4914 5359 Size: 1255 1017 651 1311 17 153 185 445 Cuts at: 5359 325 Size: 1498 Fragments arranged by size: 1498 1311 1255 1017 651 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2959 3232 4778 Size: 274 80 33 51 1443 982 273 1546 Cuts at: 4778 6490 96 Size: 1712 138 Fragments arranged by size: 1712 1546 1443 982 274 273 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3305 3337 6492 6524 3305 Size: 32 3155 32 3313 Fragments arranged by size: 3313 3155 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6532 XbaI T'CTAG_A Cuts at: 3868 3868 Size: 6532 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6532 XmaI C'CCGG_G Cuts at: 1870 3857 1870 Size: 1987 4545 XmnI GAAnn'nnTTC Cuts at: 2112 4661 2112 Size: 2549 3983 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2744 4340 276 Size: 53 83 186 936 1210 1596 2468 Fragments arranged by size: 2468 1596 1210 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI BaeI BbvCI BfrBI BplI BsiWI BsmBI EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SrfI SwaI XcmI