Restriction map for
pFN21AB5990
(Linear) (Six-base) MAPSORT of: KIBB5990.seq Check: 2304 from: 1 to: 1827
KIBB5990.orf
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 11 1210 1827
Size: 11 1199 617
Fragments arranged by size:
1199 617 11
AatII G_ACGT'C
Cuts at: 0 741 1827
Size: 741 1086
AccI GT'mk_AC
Cuts at: 0 379 1827
Size: 379 1448
AclI AA'CG_TT
Cuts at: 0 671 1434 1827
Size: 671 763 393
Fragments arranged by size:
763 671 393
AlwNI CAG_nnn'CTG
Cuts at: 0 759 1827
Size: 759 1068
ApoI r'AATT_y
Cuts at: 0 475 1827
Size: 475 1352
AvaI C'yCGr_G
Cuts at: 0 1322 1827
Size: 1322 505
AvrII C'CTAG_G
Cuts at: 0 1497 1827
Size: 1497 330
BamHI G'GATC_C
Cuts at: 0 1123 1827
Size: 1123 704
BanI G'GyrC_C
Cuts at: 0 223 1827
Size: 223 1604
BbeI G_GCGC'C
Cuts at: 0 227 1827
Size: 227 1600
BbsI GAAGACnn'nnnn_
Cuts at: 0 1353 1711 1827
Size: 1353 358 116
Fragments arranged by size:
1353 358 116
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 751 785 1827
Size: 751 34 1042
Fragments arranged by size:
1042 751 34
BlpI GC'TnA_GC
Cuts at: 0 1561 1827
Size: 1561 266
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 427 1589 1827
Size: 427 1162 238
Fragments arranged by size:
1162 427 238
Bpu10I CC'TnA_GC
Cuts at: 0 556 1827
Size: 556 1271
BsaI GGTCTCn'nnnn_
Cuts at: 0 77 918 1607 1827
Size: 77 841 689 220
Fragments arranged by size:
841 689 220 77
BsaHI Gr'CG_yC
Cuts at: 0 224 738 1827
Size: 224 514 1089
Fragments arranged by size:
1089 514 224
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1061 1091 1300 1330 1827
Size: 1061 30 209 30 497
Fragments arranged by size:
1061 497 209 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 430 1406 1827
Size: 430 976 421
Fragments arranged by size:
976 430 421
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 57 999 1526 1827
Size: 57 942 527 301
Fragments arranged by size:
942 527 301 57
BsmI GAATG_Cn'
Cuts at: 0 515 850 1827
Size: 515 335 977
Fragments arranged by size:
977 515 335
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 11 126 1210 1827
Size: 11 115 1084 617
Fragments arranged by size:
1084 617 115 11
BsrDI GCAATG_nn'
Cuts at: 0 414 1827
Size: 414 1413
BstXI CCAn_nnnn'nTGG
Cuts at: 0 798 1827
Size: 798 1029
BstYI r'GATC_y
Cuts at: 0 495 1123 1827
Size: 495 628 704
Fragments arranged by size:
704 628 495
BstZ17I GTA'TAC
Cuts at: 0 380 1827
Size: 380 1447
Bsu36I CC'TnA_GG
Cuts at: 0 1445 1827
Size: 1445 382
BtgI C'CryG_G
Cuts at: 0 928 1827
Size: 928 899
BtsI GCAGTG_nn'
Cuts at: 0 684 707 987 1185 1572 1655 1827
Size: 684 23 280 198 387 83 172
Fragments arranged by size:
684 387 280 198 172 83 23
DraIII CAC_nnn'GTG
Cuts at: 0 1810 1827
Size: 1810 17
EaeI y'GGCC_r
Cuts at: 0 1257 1613 1827
Size: 1257 356 214
Fragments arranged by size:
1257 356 214
EarI CTCTTCn'nnn_
Cuts at: 0 395 425 605 1827
Size: 395 30 180 1222
Fragments arranged by size:
1222 395 180 30
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 427 1589 1827
Size: 427 1162 238
Fragments arranged by size:
1162 427 238
EcoO109I rG'GnC_Cy
Cuts at: 0 1481 1827
Size: 1481 346
EcoRI G'AATT_C
Cuts at: 0 475 1827
Size: 475 1352
FspI TGC'GCA
Cuts at: 0 19 1827
Size: 19 1808
HaeII r_GCGC'y
Cuts at: 0 227 1827
Size: 227 1600
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 637 669 1061 1093 1111 1143 1255 1284
Size: 637 32 392 32 18 32 112 29
Cuts at: 1284 1287 1316 1396 1401 1428 1433 1827
Size: 3 29 80 5 27 5 394
Fragments arranged by size:
637 394 392 112 80 32 32 32
29 29 27 18 5 5 3
KasI G'GCGC_C
Cuts at: 0 223 1827
Size: 223 1604
MscI TGG'CCA
Cuts at: 0 1259 1615 1827
Size: 1259 356 212
Fragments arranged by size:
1259 356 212
MslI CAynn'nnrTG
Cuts at: 0 540 1827
Size: 540 1287
MspA1I CmG'CkG
Cuts at: 0 71 430 447 1827
Size: 71 359 17 1380
Fragments arranged by size:
1380 359 71 17
NarI GG'CG_CC
Cuts at: 0 224 1827
Size: 224 1603
NspI r_CATG'y
Cuts at: 0 129 769 1827
Size: 129 640 1058
Fragments arranged by size:
1058 640 129
PflMI CCAn_nnn'nTGG
Cuts at: 0 1031 1598 1739 1827
Size: 1031 567 141 88
Fragments arranged by size:
1031 567 141 88
PpuMI rG'GwC_Cy
Cuts at: 0 1481 1827
Size: 1481 346
PstI C_TGCA'G
Cuts at: 0 764 1207 1827
Size: 764 443 620
Fragments arranged by size:
764 620 443
PvuII CAG'CTG
Cuts at: 0 430 447 1827
Size: 430 17 1380
Fragments arranged by size:
1380 430 17
SanDI GG'GwC_CC
Cuts at: 0 1481 1827
Size: 1481 346
SbfI CC_TGCA'GG
Cuts at: 0 1207 1827
Size: 1207 620
ScaI AGT'ACT
Cuts at: 0 956 1229 1827
Size: 956 273 598
Fragments arranged by size:
956 598 273
SfcI C'TryA_G
Cuts at: 0 264 760 1203 1391 1466 1532 1553 1827
Size: 264 496 443 188 75 66 21 274
Fragments arranged by size:
496 443 274 264 188 75 66 21
SfoI GGC'GCC
Cuts at: 0 225 1827
Size: 225 1602
SphI G_CATG'C
Cuts at: 0 129 1827
Size: 129 1698
StyI C'CwwG_G
Cuts at: 0 78 161 916 1497 1827
Size: 78 83 755 581 330
Fragments arranged by size:
755 581 330 83 78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 592 1243 1827
Size: 592 651 584
Fragments arranged by size:
651 592 584
TatI w'GTAC_w
Cuts at: 0 954 1227 1827
Size: 954 273 600
Fragments arranged by size:
954 600 273
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1300 1332 1827
Size: 1300 32 495
Fragments arranged by size:
1300 495 32
XmnI GAAnn'nnTTC
Cuts at: 0 107 1827
Size: 107 1720
ZraI GAC'GTC
Cuts at: 0 739 1827
Size: 739 1088
Enzymes that do cut:
AarI AatII AccI AclI AlwNI ApoI AvaI AvrII
BamHI BanI BbeI BbsI BcgI BlpI BpmI Bpu10I
BsaI BsaHI BsaXI BseRI BsgI BsmI BspMI BsrDI
BstXI BstYI BstZ17I Bsu36I BtgI BtsI DraIII EaeI
EarI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I KasI
MscI MslI MspA1I NarI NspI PflMI PpuMI PstI
PvuII SanDI SbfI ScaI SfcI SfoI SphI StyI
TaqII TatI TstI XmnI ZraI
Enzymes that do not cut:
Acc65I AcuI AfeI AflII AflIII AgeI AhdI AleI
AlfI AloI ApaI ApaLI AscI AseI AsiSI BaeI
BanII BbvCI BciVI BclI BfrBI BglI BglII Bme1580I
BmgBI BmrI BmtI BplI BpuEI BsaAI BsaBI BsaWI
BseYI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspEI BspHI
BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BtgZI ClaI CspCI DraI DrdI EagI EciI EcoICRI
EcoNI EcoRV FalI FseI FspAI HincII HindIII HpaI
KpnI MfeI MluI MmeI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PciI PfoI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SacI SacII SalI SapI SexAI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI SspI
StuI SwaI Tth111I XbaI XcmI XhoI XmaI