Restriction map for
pFN21AB5982
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# NO CUT ENZYMES ( 111 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI Alw44I AlwNI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AviII BamHI BbeI
BbrPI BbsI BcgI BciVI BclI
BfrI BglI BglII BpuAI BsaAI
BsaBI BsaI BseAI BseRI BsiEI
BsiWI BsmI BspDI BspEI BsrDI
BsrFI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I Cfr10I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco52I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HindIII HpaI KasI KpnI
KspI MamI MluI MroI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmlI SnaBI
Sse8387I SspI SwaI Van91I XcmI
XhoI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccII 1696 CGCG CG^CG
AcyI 1172 GGCGTC G[GA]^CG[CT]C
AflIII 288 ACATGT A^C[GA][CT]GT
AocI 75 CCTCAGG CC^T[ACGT]AGG
AspHI 193 GAGCTC G[AT]GC[AT]^C
AspI 1103 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1244 CCCGGG C^[CT]CG[GA]G
AvrII 828 CCTAGG C^CTAGG
BanI 1252 GGTGCC G^G[CT][GA]CC
BbvCI 460 GCTGAGG CCTCAGC(-5/-2)
BlnI 828 CCTAGG C^CTAGG
BlpI 712 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 712 GCTGAGC GC^T[ACGT]AGC
BsaHI 1172 GGCGTC G[GA]^CG[CT]C
BsaWI 986 ACCGGA [AT]^CCGG[AT]
BsgI 1394 GTGCAG GTGCAG(16/14)
BsiHKAI 193 GAGCTC G[AT]GC[AT]^C
BsmBI 954 GAGACG CGTCTC(1/5)
BsoBI 1244 CCCGGG C^[CT]CG[GA]G
BspHI 981 TCATGA T^CATGA
BspLU11I 288 ACATGT A^CATGT
BspMI 650 ACCTGC ACCTGC(4/8)
BsrBI 628 GAGCGG CCGCTC(-3/-3)
BstUI 1696 CGCG CG^CG
Bsu36I 75 CCTCAGG CC^T[ACGT]AGG
CelII 712 GCTGAGC GC^T[ACGT]AGC
DraII 807 GGGACCC [GA]G^G[ACGT]CC[CT]
Eco81I 75 CCTCAGG CC^T[ACGT]AGG
EcoNI 1342 CCTCATACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 807 GGGACCC [GA]G^G[ACGT]CC[CT]
HgaI 1173 GCGTC GACGC(5/10)
Hin1I 1172 GGCGTC G[GA]^CG[CT]C
MseI 1318 TTAA T^TAA
MvnI 1696 CGCG CG^CG
PflFI 1103 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 807 GGGACCC [GA]G^G[AT]CC[CT]
PshAI 1500 GACAACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 323 CTGCAG CTGCA^G
RcaI 981 TCATGA T^CATGA
SacI 193 GAGCTC GAGCT^C
SmaI 1244 CCCGGG CCC^GGG
SpeI 187 ACTAGT A^CTAGT
StuI 781 AGGCCT AGG^CCT
TaqI 1631 TCGA T^CGA
Tru9I 1318 TTAA T^TAA
Tth111I 1103 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1631 TCGA T^CGA
XbaI 1044 TCTAGA T^CTAGA
XmaCI 1244 CCCGGG C^CCGGG
XmaI 1244 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 40 enzymes) #
###################################
Aor51HI 1190 AGCGCT AGC^GCT
1667 AGCGCT
Asp700I 123 GAAAGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
313 GAACTGCTTC
AvaII 390 GGACC G^G[AT]CC
808 GGACC
BalI 1268 TGGCCA TGG^CCA
1376 TGGCCA
BanII 193 GAGCTC G[GA]GC[CT]^C
821 GGGCTC
BcnI 1244 CCCGG CC^[GC]GG
1245 CCGGG
BmyI 193 GAGCTC G[AGT]GC[ACT]^C
821 GGGCTC
BpmI 1115 CTGGAG CTGGAG(16/14)
1151 CTGGAG
BsiYI 812 CCAGGACAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1342 CCTCATACAGG
BslI 812 CCAGGACAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1342 CCTCATACAGG
BsmFI 807 GGGAC GGGAC(10/14)
975 GTCCC
Bsp1286I 193 GAGCTC G[AGT]GC[ACT]^C
821 GGGCTC
Bsp1407I 272 TGTACA T^GTACA
306 TGTACA
BsrGI 272 TGTACA T^GTACA
306 TGTACA
BstXI 884 CCATTGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1121 CCACCACCCTGG
BstYI 747 GGATCT [GA]^GATC[CT]
989 GGATCT
CfoI 1191 GCGC GCG^C
1668 GCGC
DsaI 248 CCATGG C^C[GA][CT]GG
1655 CCATGG
Eco47III 1190 AGCGCT AGC^GCT
1667 AGCGCT
FauI 1022 CCCGC CCCGC(4/6)
630 GCGGG
HaeII 1190 AGCGCT [GA]GCGC^[CT]
1667 AGCGCT
HhaI 1191 GCGC GCG^C
1668 GCGC
HinP1I 1191 GCGC G^CGC
1668 GCGC
HincII 220 GTTGAC GT[CT]^[GA]AC
442 GTTGAC
HindII 220 GTTGAC GT[CT]^[GA]AC
442 GTTGAC
HphI 609 GGTGA GGTGA(8/7)
1508 TCACC
MaeII 957 ACGT A^CGT
995 ACGT
MfeI 435 CAATTG C^AATTG
1571 CAATTG
MflI 747 GGATCT [GA]^GATC[CT]
989 GGATCT
MluNI 1268 TGGCCA TGG^CCA
1376 TGGCCA
MscI 1268 TGGCCA TGG^CCA
1376 TGGCCA
MunI 435 CAATTG C^AATTG
1571 CAATTG
NciI 1244 CCCGG CC^[GC]GG
1245 CCGGG
NcoI 248 CCATGG C^CATGG
1655 CCATGG
PvuII 458 CAGCTG CAG^CTG
1635 CAGCTG
SapI 81 GAAGAGC GCTCTTC(1/4)
577 GAAGAGC
SphI 615 GCATGC GCATG^C
1220 GCATGC
SspBI 272 TGTACA T^GTACA
306 TGTACA
XhoII 747 GGATCT [GA]^GATC[CT]
989 GGATCT
XmnI 123 GAAAGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
313 GAACTGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI BbvI BfaI BsaJI BsmAI
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII EaeI EarI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HinfI HpaII ItaI Ksp632I
MaeI MaeIII MboI MboII MnlI
MslI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV NspI PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TfiI TseI Tsp45I
Tsp509I TspRI