(Circular) (Six-base) MAPSORT of: pFN21AB5982.seq Check: 6011 from: 1 to: 6409 pFN21AB5982 6409 bp With 182 enzymes: SgfI * April 15, 2010 09:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4219 278 Size: 53 83 186 936 2683 2468 Fragments arranged by size: 2683 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3752 1937 Size: 1815 4594 Acc65I G'GTAC_C Cuts at: 3730 3730 Size: 6409 AclI AA'CG_TT Cuts at: 1563 4536 4909 1563 Size: 2973 373 3063 Fragments arranged by size: 3063 2973 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2327 2514 2601 4472 5856 6124 1632 Size: 695 187 87 1871 1384 268 1917 Fragments arranged by size: 1917 1871 1384 695 268 187 87 AfeI AGC'GCT Cuts at: 3197 3674 3197 Size: 477 5932 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6212 AflIII A'CryG_T Cuts at: 1203 2293 5308 6285 1203 Size: 1090 3015 977 1327 Fragments arranged by size: 3015 1327 1090 977 AgeI A'CCGG_T Cuts at: 5240 5240 Size: 6409 AhdI GACnn_n'nnGTC Cuts at: 5138 5138 Size: 6409 AleI CACnn'nnGTG Cuts at: 1166 2442 1166 Size: 1276 5133 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6409 AlwNI CAG_nnn'CTG Cuts at: 4152 5724 4152 Size: 1572 4837 ApaLI G'TGCA_C Cuts at: 4466 5622 4466 Size: 1156 5253 ApoI r'AATT_y Cuts at: 1493 2259 2565 3325 3718 3944 4096 6205 1493 Size: 766 306 760 393 226 152 2109 1697 Fragments arranged by size: 2109 1697 766 760 393 306 226 152 AseI AT'TA_AT Cuts at: 160 1032 4963 160 Size: 872 3931 1606 Fragments arranged by size: 3931 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6409 AvaI C'yCGr_G Cuts at: 1870 1943 3249 3734 1870 Size: 73 1306 485 4545 Fragments arranged by size: 4545 1306 485 73 AvrII C'CTAG_G Cuts at: 2833 2833 Size: 6409 BamHI G'GATC_C Cuts at: 3739 4106 3739 Size: 367 6042 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3257 3730 5185 618 Size: 325 217 627 1470 473 1455 1842 Fragments arranged by size: 1842 1470 1455 627 473 325 217 BanII G_rGCy'C Cuts at: 1391 2202 2830 3728 1391 Size: 811 628 898 4072 Fragments arranged by size: 4072 898 811 628 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5438 BbvCI CC'TCA_GC Cuts at: 2466 2466 Size: 6409 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4600 4634 1903 Size: 34 2663 34 3678 Fragments arranged by size: 3678 2663 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4301 5511 4301 Size: 1210 5199 BclI T'GATC_A Cuts at: 1548 1548 Size: 6409 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5020 136 Size: 107 122 71 631 605 3348 1525 Fragments arranged by size: 3348 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6404 6404 Size: 6409 BlpI GC'TnA_GC Cuts at: 2718 3826 2718 Size: 1108 5301 Bme1580I G_kGCm'C Cuts at: 1790 4470 5626 1790 Size: 2680 1156 2573 Fragments arranged by size: 2680 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6409 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2620 4140 5098 Size: 416 53 83 399 24 1203 1520 958 Cuts at: 5098 442 Size: 1753 Fragments arranged by size: 1753 1520 1203 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6409 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3141 3177 5069 1239 Size: 1902 36 1892 2579 Fragments arranged by size: 2579 1902 1892 36 Bpu10I CC'TnA_GC Cuts at: 2466 2466 Size: 6409 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4534 5399 5697 4534 Size: 865 298 5246 Fragments arranged by size: 5246 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5072 882 Size: 617 3573 2219 Fragments arranged by size: 3573 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4200 493 Size: 711 2996 2702 Fragments arranged by size: 2996 2702 711 BsaBI GATnn'nnATC Cuts at: 6403 6403 Size: 6409 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3178 4216 4598 5233 Size: 53 83 186 936 1645 1038 382 635 Cuts at: 5233 275 Size: 1451 Fragments arranged by size: 1645 1451 1038 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2991 4842 5240 5514 5661 6072 2991 Size: 1851 398 274 147 411 3328 Fragments arranged by size: 3328 1851 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6184 Fragments arranged by size: 6184 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6409 BseYI C'CCAG_C Cuts at: 3229 3642 3669 5612 6108 3229 Size: 413 27 1943 496 3530 Fragments arranged by size: 3530 1943 496 413 27 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3420 1163 Size: 573 1684 4152 Fragments arranged by size: 4152 1684 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3859 4620 5648 1540 Size: 275 186 1858 761 1028 2301 Fragments arranged by size: 2301 1858 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2202 4470 4555 5626 2202 Size: 2268 85 1071 2985 Fragments arranged by size: 2985 2268 1071 85 BsmI GAATG_Cn' Cuts at: 3920 4013 3920 Size: 93 6316 BsmBI CGTCTCn'nnnn_ Cuts at: 2953 2953 Size: 6409 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2202 2830 3728 4470 4555 5626 1391 Size: 399 412 628 898 742 85 1071 2174 Fragments arranged by size: 2174 1071 898 742 628 412 399 85 BspEI T'CCGG_A Cuts at: 6072 6072 Size: 6409 BspHI T'CATG_A Cuts at: 2986 4298 6028 2986 Size: 1312 1730 3367 Fragments arranged by size: 3367 1730 1312 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2664 3764 844 Size: 1820 1100 3489 Fragments arranged by size: 3489 1820 1100 BsrBI CCG'CTC Cuts at: 1394 1823 2635 4296 1394 Size: 429 812 1661 3507 Fragments arranged by size: 3507 1661 812 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4904 5078 65 Size: 1183 3656 174 1396 Fragments arranged by size: 3656 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5053 5240 1700 Size: 110 3243 187 2869 Fragments arranged by size: 3243 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2277 2311 96 Size: 2181 34 4194 Fragments arranged by size: 4194 2181 34 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6409 BssSI C'ACGA_G Cuts at: 4469 5481 4469 Size: 1012 5397 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6409 BstBI TT'CG_AA Cuts at: 5213 5213 Size: 6409 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6409 BstXI CCAn_nnnn'nTGG Cuts at: 2896 3133 2896 Size: 237 6172 BstYI r'GATC_y Cuts at: 1972 2752 2994 3739 4106 4491 4508 5960 6046 Size: 780 242 745 367 385 17 1452 86 Cuts at: 6046 6058 6404 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 780 745 385 367 346 242 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2081 2081 Size: 6409 BtgI C'CryG_G Cuts at: 513 1065 2004 2253 3660 513 Size: 552 939 249 1407 3262 Fragments arranged by size: 3262 1407 939 552 249 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5670 Fragments arranged by size: 5670 683 56 BtsI GCAGTG_nn' Cuts at: 3925 4719 4739 3925 Size: 794 20 5595 Fragments arranged by size: 5595 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6409 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6374 DraI TTT'AAA Cuts at: 3713 3713 Size: 6409 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5416 809 Size: 507 4100 1802 Fragments arranged by size: 4100 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3019 3273 3381 Size: 54 1425 50 275 18 1189 254 108 Cuts at: 3381 3856 4745 6374 8 Size: 475 889 1629 43 Fragments arranged by size: 1629 1425 1189 889 475 275 254 108 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3856 1537 Size: 275 2044 4090 Fragments arranged by size: 4090 2044 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2081 2505 2577 4339 1353 Size: 728 424 72 1762 3423 Fragments arranged by size: 3423 1762 728 424 72 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4996 5370 5516 387 Size: 1064 138 3407 374 146 1280 Fragments arranged by size: 3407 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2200 2200 Size: 6409 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2327 2514 2601 3141 3177 4472 5069 Size: 393 695 187 87 540 36 1295 597 Cuts at: 5069 5856 6124 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1295 787 695 597 540 393 268 187 87 36 EcoNI CCTnn'n_nnAGG Cuts at: 3351 3351 Size: 6409 EcoO109I rG'GnC_Cy Cuts at: 2813 2813 Size: 6409 EcoRI G'AATT_C Cuts at: 3718 4096 3718 Size: 378 6031 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2339 2339 Size: 6409 FspI TGC'GCA Cuts at: 4915 4915 Size: 6409 HaeII r_GCGC'y Cuts at: 1678 3199 3676 5556 1678 Size: 1521 477 1880 2531 Fragments arranged by size: 2531 1880 1521 477 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2029 2061 2812 2844 3129 Size: 32 135 32 457 32 751 32 285 Cuts at: 3129 3161 3277 3309 5050 5082 5124 5156 1373 Size: 32 116 32 1741 32 42 32 2626 Fragments arranged by size: 2626 1741 751 457 285 135 116 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2227 2449 3753 3999 669 Size: 988 281 289 222 1304 246 3079 Fragments arranged by size: 3079 1304 988 289 281 246 222 HindIII A'AGCT_T Cuts at: 748 748 Size: 6409 HpaI GTT'AAC Cuts at: 3999 3999 Size: 6409 KpnI G_GTAC'C Cuts at: 3734 3734 Size: 6409 MfeI C'AATT_G Cuts at: 2440 3576 4008 2440 Size: 1136 432 4841 Fragments arranged by size: 4841 1136 432 MluI A'CGCG_T Cuts at: 6285 6285 Size: 6409 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2373 3130 5523 5707 1305 Size: 1068 757 2393 184 2007 Fragments arranged by size: 2393 2007 1068 757 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3275 3383 10 Size: 54 1425 343 1443 108 3036 Fragments arranged by size: 3036 1443 1425 343 108 54 MslI CAynn'nnrTG Cuts at: 518 1166 2442 2894 3188 3429 3518 4367 4726 Size: 648 1276 452 294 241 89 849 359 Cuts at: 4726 4885 518 Size: 159 2042 Fragments arranged by size: 2042 1276 849 648 452 359 294 241 159 89 MspA1I CmG'CkG Cuts at: 1606 2465 2733 3642 3825 4502 5650 5895 1606 Size: 859 268 909 183 677 1148 245 2120 Fragments arranged by size: 2120 1148 909 859 677 268 245 183 NcoI C'CATG_G Cuts at: 513 1065 2004 2253 3660 513 Size: 552 939 249 1407 3262 Fragments arranged by size: 3262 1407 939 552 249 NdeI CA'TA_TG Cuts at: 387 387 Size: 6409 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6409 NotI GC'GGCC_GC Cuts at: 3856 3856 Size: 6409 NspI r_CATG'y Cuts at: 1132 1229 2297 2624 3229 3767 5312 1132 Size: 97 1068 327 605 538 1545 2229 Fragments arranged by size: 2229 1545 1068 605 538 327 97 PasI CC'CwG_GG Cuts at: 1348 3133 1348 Size: 1785 4624 PciI A'CATG_T Cuts at: 2293 5308 2293 Size: 3015 3394 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6409 PfoI T'CCnGG_A Cuts at: 3500 3500 Size: 6409 PmeI GTTT'AAAC Cuts at: 3713 3713 Size: 6409 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4478 4510 5991 6023 4478 Size: 32 1481 32 4864 Fragments arranged by size: 4864 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2813 2813 Size: 6409 PshAI GACnn'nnGTC Cuts at: 1613 3509 1613 Size: 1896 4513 PsiI TTA'TAA Cuts at: 3979 3979 Size: 6409 PstI C_TGCA'G Cuts at: 830 2332 3761 830 Size: 1502 1429 3478 Fragments arranged by size: 3478 1502 1429 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6409 PvuII CAG'CTG Cuts at: 2465 3642 2465 Size: 1177 5232 SacI G_AGCT'C Cuts at: 2202 2202 Size: 6409 SalI G'TCGA_C Cuts at: 1936 3751 1936 Size: 1815 4594 SanDI GG'GwC_CC Cuts at: 2813 2813 Size: 6409 SapI GCTCTTCn'nnn_ Cuts at: 2081 2577 2081 Size: 496 5913 SbfI CC_TGCA'GG Cuts at: 3761 3761 Size: 6409 ScaI AGT'ACT Cuts at: 4657 6369 4657 Size: 1712 4697 SfcI C'TryA_G Cuts at: 826 1045 2164 2328 2660 2734 3757 4892 5573 Size: 219 1119 164 332 74 1023 1135 681 Cuts at: 5573 5764 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1119 1023 681 332 219 191 164 74 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6409 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6409 SmaI CCC'GGG Cuts at: 1872 3251 3736 1872 Size: 1379 485 4545 Fragments arranged by size: 4545 1379 485 SmlI C'TyrA_G Cuts at: 820 1017 1943 4513 5414 5676 820 Size: 197 926 2570 901 262 1553 Fragments arranged by size: 2570 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4200 493 Size: 3707 2702 SpeI A'CTAG_T Cuts at: 152 2192 152 Size: 2040 4369 SphI G_CATG'C Cuts at: 1132 2624 3229 3767 1132 Size: 1492 605 538 3774 Fragments arranged by size: 3774 1492 605 538 SspI AAT'ATT Cuts at: 5 52 4333 5 Size: 47 4281 2081 Fragments arranged by size: 4281 2081 47 StuI AGG'CCT Cuts at: 1517 2788 1517 Size: 1271 5138 StyI C'CwwG_G Cuts at: 513 1065 2004 2233 2253 2833 3660 3848 513 Size: 552 939 229 20 580 827 188 3074 Fragments arranged by size: 3074 939 827 580 552 229 188 20 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2602 4436 4453 4606 4791 5236 325 Size: 1255 1022 1834 17 153 185 445 1498 Fragments arranged by size: 1834 1498 1255 1022 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2277 2311 4655 Size: 274 80 33 51 1443 300 34 2344 Cuts at: 4655 6367 96 Size: 1712 138 Fragments arranged by size: 2344 1712 1443 300 274 138 80 51 34 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3255 3287 3452 3484 6369 6401 3255 Size: 32 165 32 2885 32 3263 Fragments arranged by size: 3263 2885 165 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3111 1316 Size: 1795 4614 XbaI T'CTAG_A Cuts at: 3049 3745 3049 Size: 696 5713 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6409 XmaI C'CCGG_G Cuts at: 1870 3249 3734 1870 Size: 1379 485 4545 Fragments arranged by size: 4545 1379 485 XmnI GAAnn'nnTTC Cuts at: 2132 2322 4538 2132 Size: 190 2216 4003 Fragments arranged by size: 4003 2216 190 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4217 276 Size: 53 83 186 936 2683 2468 Fragments arranged by size: 2683 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI BaeI BbeI BfrBI BplI BsiWI BstZ17I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SexAI SfiI SfoI SrfI SwaI XcmI