(Circular) (Six-base) MAPSORT of: pFN21AB5981.seq Check: 1435 from: 1 to: 6088 pFN21AB5981 6088 bp With 182 enzymes: SgfI * April 15, 2010 09:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3898 278 Size: 53 83 186 936 2362 2468 Fragments arranged by size: 2468 2362 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2638 3431 1937 Size: 701 793 4594 Fragments arranged by size: 4594 793 701 Acc65I G'GTAC_C Cuts at: 3409 3409 Size: 6088 AclI AA'CG_TT Cuts at: 1563 4215 4588 1563 Size: 2652 373 3063 Fragments arranged by size: 3063 2652 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2098 2236 2683 3103 3187 3271 3351 4151 Size: 466 138 447 420 84 84 80 800 Cuts at: 4151 5535 5803 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 800 466 447 420 268 138 84 84 80 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5891 AflIII A'CryG_T Cuts at: 1203 2423 2559 4987 5964 1203 Size: 1220 136 2428 977 1327 Fragments arranged by size: 2428 1327 1220 977 136 AgeI A'CCGG_T Cuts at: 4919 4919 Size: 6088 AhdI GACnn_n'nnGTC Cuts at: 4817 4817 Size: 6088 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6088 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6088 AlwNI CAG_nnn'CTG Cuts at: 3831 5403 3831 Size: 1572 4516 ApaLI G'TGCA_C Cuts at: 4145 5301 4145 Size: 1156 4932 ApoI r'AATT_y Cuts at: 1493 2175 3153 3321 3397 3623 3775 5884 1493 Size: 682 978 168 76 226 152 2109 1697 Fragments arranged by size: 2109 1697 978 682 226 168 152 76 AseI AT'TA_AT Cuts at: 160 1032 4642 160 Size: 872 3610 1606 Fragments arranged by size: 3610 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6088 AvaI C'yCGr_G Cuts at: 1870 1943 3413 1870 Size: 73 1470 4545 Fragments arranged by size: 4545 1470 73 BamHI G'GATC_C Cuts at: 2068 3418 3785 2068 Size: 1350 367 4371 Fragments arranged by size: 4371 1350 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3409 4864 618 Size: 325 217 627 1622 1455 1842 Fragments arranged by size: 1842 1622 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3407 1391 Size: 2016 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2154 2169 2560 2980 928 Size: 971 255 15 391 420 4036 Fragments arranged by size: 4036 971 420 391 255 15 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4279 4313 1903 Size: 34 2342 34 3678 Fragments arranged by size: 3678 2342 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3980 5190 3980 Size: 1210 4878 BclI T'GATC_A Cuts at: 1548 1548 Size: 6088 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4699 136 Size: 107 122 71 631 605 3027 1525 Fragments arranged by size: 3027 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6083 6083 Size: 6088 BlpI GC'TnA_GC Cuts at: 3505 3505 Size: 6088 Bme1580I G_kGCm'C Cuts at: 1790 4149 5305 1790 Size: 2359 1156 2573 Fragments arranged by size: 2573 2359 1156 BmgBI CAC'GTC Cuts at: 1318 2381 1318 Size: 1063 5025 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2619 3039 3371 Size: 416 53 83 399 24 1202 420 332 Cuts at: 3371 3819 4777 442 Size: 448 958 1753 Fragments arranged by size: 1753 1202 958 448 420 416 399 332 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6088 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2069 2313 2511 2679 2763 3099 3183 3267 Size: 830 244 198 168 84 336 84 84 Cuts at: 3267 3351 4748 1239 Size: 84 1397 2579 Fragments arranged by size: 2579 1397 830 336 244 198 168 84 84 84 84 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2467 4213 5078 5376 2467 Size: 1746 865 298 3179 Fragments arranged by size: 3179 1746 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4751 882 Size: 617 3252 2219 Fragments arranged by size: 3252 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2426 2474 3879 493 Size: 711 1222 48 1405 2702 Fragments arranged by size: 2702 1405 1222 711 48 BsaBI GATnn'nnATC Cuts at: 6082 6082 Size: 6088 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3895 4277 4912 275 Size: 53 83 186 936 2362 382 635 1451 Fragments arranged by size: 2362 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2909 4521 4919 5193 5340 5751 2909 Size: 1612 398 274 147 411 3246 Fragments arranged by size: 3246 1612 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5863 Fragments arranged by size: 5863 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2066 1186 Size: 880 5208 BseYI C'CCAG_C Cuts at: 5291 5787 5291 Size: 496 5592 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5515 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3538 4299 5327 1540 Size: 275 186 1537 761 1028 2301 Fragments arranged by size: 2301 1537 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4149 4234 5305 4149 Size: 85 1071 4932 Fragments arranged by size: 4932 1071 85 BsmI GAATG_Cn' Cuts at: 2547 2681 2765 2942 3599 3692 2547 Size: 134 84 177 657 93 4943 Fragments arranged by size: 4943 657 177 134 93 84 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3407 4149 4234 5305 1391 Size: 399 1617 742 85 1071 2174 Fragments arranged by size: 2174 1617 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5751 5751 Size: 6088 BspHI T'CATG_A Cuts at: 3977 5707 3977 Size: 1730 4358 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3443 844 Size: 2599 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3975 1394 Size: 429 2152 3507 Fragments arranged by size: 3507 2152 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2690 3004 3110 3278 4583 4757 65 Size: 1183 1442 314 106 168 1305 174 1396 Fragments arranged by size: 1442 1396 1305 1183 314 174 168 106 BsrFI r'CCGG_y Cuts at: 1700 1810 4732 4919 1700 Size: 110 2922 187 2869 Fragments arranged by size: 2922 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3182 96 Size: 3086 3002 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6088 BssSI C'ACGA_G Cuts at: 2894 4148 5160 2894 Size: 1254 1012 3822 Fragments arranged by size: 3822 1254 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6088 BstBI TT'CG_AA Cuts at: 2551 2803 2887 3055 3139 4892 2551 Size: 252 84 168 84 1753 3747 Fragments arranged by size: 3747 1753 252 168 84 84 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6088 BstYI r'GATC_y Cuts at: 1972 2068 3418 3785 4170 4187 5639 5725 5737 Size: 96 1350 367 385 17 1452 86 12 Cuts at: 5737 6083 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1350 385 367 346 96 86 17 12 BstZ17I GTA'TAC Cuts at: 2639 2639 Size: 6088 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4597 Fragments arranged by size: 4597 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2441 487 Size: 683 56 1215 4134 Fragments arranged by size: 4134 1215 683 56 BtsI GCAGTG_nn' Cuts at: 2320 2774 3604 4398 4418 2320 Size: 454 830 794 20 3990 Fragments arranged by size: 3990 830 794 454 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6088 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2668 2703 2752 2787 529 Size: 35 2104 35 49 35 3830 Fragments arranged by size: 3830 2104 49 35 35 35 DraI TTT'AAA Cuts at: 3392 3392 Size: 6088 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5095 809 Size: 507 3779 1802 Fragments arranged by size: 3779 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3535 4424 6053 Size: 54 1425 50 275 18 1705 889 1629 Cuts at: 6053 8 Size: 43 Fragments arranged by size: 1705 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3535 1537 Size: 275 1723 4090 Fragments arranged by size: 4090 1723 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4018 1353 Size: 2665 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4675 5049 5195 387 Size: 1064 138 3086 374 146 1280 Fragments arranged by size: 3086 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2069 2098 2236 2313 2511 2679 2683 Size: 393 437 29 138 77 198 168 4 Cuts at: 2683 2763 3099 3103 3183 3187 3267 3271 3351 Size: 80 336 4 80 4 80 4 80 Cuts at: 3351 4151 4748 5535 5803 1239 Size: 800 597 787 268 1524 Fragments arranged by size: 1524 800 787 597 437 393 336 268 198 168 138 80 80 80 80 77 29 4 4 4 4 EcoO109I rG'GnC_Cy Cuts at: 2339 3069 2339 Size: 730 5358 EcoRI G'AATT_C Cuts at: 3153 3397 3775 3153 Size: 244 378 5466 Fragments arranged by size: 5466 378 244 FspI TGC'GCA Cuts at: 4594 4594 Size: 6088 HaeII r_GCGC'y Cuts at: 1678 5235 1678 Size: 3557 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2440 2472 4729 4761 4803 Size: 32 135 32 868 32 2257 32 42 Cuts at: 4803 4835 1373 Size: 32 2626 Fragments arranged by size: 2626 2257 868 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2565 3432 3678 669 Size: 988 281 627 867 246 3079 Fragments arranged by size: 3079 988 867 627 281 246 HindIII A'AGCT_T Cuts at: 748 2778 748 Size: 2030 4058 HpaI GTT'AAC Cuts at: 2565 3678 2565 Size: 1113 4975 KpnI G_GTAC'C Cuts at: 3413 3413 Size: 6088 MfeI C'AATT_G Cuts at: 3687 3687 Size: 6088 MluI A'CGCG_T Cuts at: 5964 5964 Size: 6088 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5202 5386 1305 Size: 3897 184 2007 Fragments arranged by size: 3897 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4266 Fragments arranged by size: 4266 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4046 4405 4564 518 Size: 648 2880 359 159 2042 Fragments arranged by size: 2880 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3504 4181 5329 5574 1606 Size: 1898 677 1148 245 2120 Fragments arranged by size: 2120 1898 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4597 Fragments arranged by size: 4597 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6088 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6088 NotI GC'GGCC_GC Cuts at: 3535 3535 Size: 6088 NspI r_CATG'y Cuts at: 1132 1229 2563 3446 4991 1132 Size: 97 1334 883 1545 2229 Fragments arranged by size: 2229 1545 1334 883 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6088 PciI A'CATG_T Cuts at: 2559 4987 2559 Size: 2428 3660 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6088 PfoI T'CCnGG_A Cuts at: 2290 2488 2290 Size: 198 5890 PmeI GTTT'AAAC Cuts at: 3392 3392 Size: 6088 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4157 4189 5670 5702 4157 Size: 32 1481 32 4543 Fragments arranged by size: 4543 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2339 3069 2339 Size: 730 5358 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6088 PsiI TTA'TAA Cuts at: 3009 3658 3009 Size: 649 5439 PstI C_TGCA'G Cuts at: 830 3440 830 Size: 2610 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6088 SalI G'TCGA_C Cuts at: 1936 3430 1936 Size: 1494 4594 SbfI CC_TGCA'GG Cuts at: 3440 3440 Size: 6088 ScaI AGT'ACT Cuts at: 4336 6048 4336 Size: 1712 4376 SfcI C'TryA_G Cuts at: 826 1045 2089 2130 3436 4571 5252 5443 826 Size: 219 1044 41 1306 1135 681 191 1471 Fragments arranged by size: 1471 1306 1135 1044 681 219 191 41 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6088 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6088 SmaI CCC'GGG Cuts at: 1872 3415 1872 Size: 1543 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2446 4192 5093 5355 820 Size: 197 926 503 1746 901 262 1553 Fragments arranged by size: 1746 1553 926 901 503 262 197 SnaBI TAC'GTA Cuts at: 493 3879 493 Size: 3386 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6088 SphI G_CATG'C Cuts at: 1132 3446 1132 Size: 2314 3774 SspI AAT'ATT Cuts at: 5 52 4012 5 Size: 47 3960 2081 Fragments arranged by size: 3960 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6088 StyI C'CwwG_G Cuts at: 513 1065 2004 3527 513 Size: 552 939 1523 3074 Fragments arranged by size: 3074 1523 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4115 4132 4285 4470 4915 325 Size: 1255 2535 17 153 185 445 1498 Fragments arranged by size: 2535 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2796 3182 3310 Size: 274 80 33 51 1443 819 386 128 Cuts at: 3310 4334 6046 96 Size: 1024 1712 138 Fragments arranged by size: 1712 1443 1024 819 386 274 138 128 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6048 6080 6048 Size: 32 6056 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6088 XbaI T'CTAG_A Cuts at: 3424 3424 Size: 6088 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2264 2011 Size: 253 5835 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6088 XmaI C'CCGG_G Cuts at: 1870 3413 1870 Size: 1543 4545 XmnI GAAnn'nnTTC Cuts at: 2696 2780 3116 3153 3200 3284 3321 4217 2696 Size: 84 336 37 47 84 37 896 4567 Fragments arranged by size: 4567 896 336 84 84 47 37 37 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3896 276 Size: 53 83 186 936 2362 2468 Fragments arranged by size: 2468 2362 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI