Restriction map for
pFN21AB5981
(Linear) (Six-base) MAPSORT of: KIBB5981.seq Check: 7711 from: 1 to: 1383
KIBB5981.orf
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AccI GT'mk_AC
Cuts at: 0 633 1383
Size: 633 750
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 93 231 678 1098 1182 1266 1346 1383
Size: 93 138 447 420 84 84 80 37
Fragments arranged by size:
447 420 138 93 84 84 80 37
AflIII A'CryG_T
Cuts at: 0 418 554 1383
Size: 418 136 829
Fragments arranged by size:
829 418 136
ApoI r'AATT_y
Cuts at: 0 170 1148 1316 1383
Size: 170 978 168 67
Fragments arranged by size:
978 170 168 67
BamHI G'GATC_C
Cuts at: 0 63 1383
Size: 63 1320
BbsI GAAGACnn'nnnn_
Cuts at: 0 149 164 555 975 1383
Size: 149 15 391 420 408
Fragments arranged by size:
420 408 391 149 15
BmgBI CAC'GTC
Cuts at: 0 376 1383
Size: 376 1007
BmrI ACTGGGnnnn_n'
Cuts at: 0 614 1034 1366 1383
Size: 614 420 332 17
Fragments arranged by size:
614 420 332 17
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 308 506 674 758 1094 1178 1262
Size: 64 244 198 168 84 336 84 84
Cuts at: 1262 1346 1383
Size: 84 37
Fragments arranged by size:
336 244 198 168 84 84 84 84
64 37
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 462 1383
Size: 462 921
BsaAI yAC'GTr
Cuts at: 0 421 469 1383
Size: 421 48 914
Fragments arranged by size:
914 421 48
BsaWI w'CCGG_w
Cuts at: 0 904 1383
Size: 904 479
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 61 1383
Size: 61 1322
BsmI GAATG_Cn'
Cuts at: 0 542 676 760 937 1383
Size: 542 134 84 177 446
Fragments arranged by size:
542 446 177 134 84
BsrDI GCAATG_nn'
Cuts at: 0 685 999 1105 1273 1383
Size: 685 314 106 168 110
Fragments arranged by size:
685 314 168 110 106
BsrGI T'GTAC_A
Cuts at: 0 1177 1383
Size: 1177 206
BssSI C'ACGA_G
Cuts at: 0 889 1383
Size: 889 494
BstBI TT'CG_AA
Cuts at: 0 546 798 882 1050 1134 1383
Size: 546 252 84 168 84 249
Fragments arranged by size:
546 252 249 168 84 84
BstYI r'GATC_y
Cuts at: 0 63 1383
Size: 63 1320
BstZ17I GTA'TAC
Cuts at: 0 634 1383
Size: 634 749
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 436 1383
Size: 436 947
BtsI GCAGTG_nn'
Cuts at: 0 315 769 1383
Size: 315 454 614
Fragments arranged by size:
614 454 315
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 663 698 747 782 1383
Size: 663 35 49 35 601
Fragments arranged by size:
663 601 49 35 35
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 93 231 308 506 674 678 758
Size: 64 29 138 77 198 168 4 80
Cuts at: 758 1094 1098 1178 1182 1262 1266 1346 1383
Size: 336 4 80 4 80 4 80 37
Fragments arranged by size:
336 198 168 138 80 80 80 80
77 64 37 29 4 4 4 4
EcoO109I rG'GnC_Cy
Cuts at: 0 334 1064 1383
Size: 334 730 319
Fragments arranged by size:
730 334 319
EcoRI G'AATT_C
Cuts at: 0 1148 1383
Size: 1148 235
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 435 467 1383
Size: 435 32 916
Fragments arranged by size:
916 435 32
HincII GTy'rAC
Cuts at: 0 560 1383
Size: 560 823
HindIII A'AGCT_T
Cuts at: 0 773 1383
Size: 773 610
HpaI GTT'AAC
Cuts at: 0 560 1383
Size: 560 823
NspI r_CATG'y
Cuts at: 0 558 1383
Size: 558 825
PciI A'CATG_T
Cuts at: 0 554 1383
Size: 554 829
PfoI T'CCnGG_A
Cuts at: 0 285 483 1383
Size: 285 198 900
Fragments arranged by size:
900 285 198
PpuMI rG'GwC_Cy
Cuts at: 0 334 1064 1383
Size: 334 730 319
Fragments arranged by size:
730 334 319
PsiI TTA'TAA
Cuts at: 0 1004 1383
Size: 1004 379
SfcI C'TryA_G
Cuts at: 0 84 125 1383
Size: 84 41 1258
Fragments arranged by size:
1258 84 41
SmlI C'TyrA_G
Cuts at: 0 441 1383
Size: 441 942
TatI w'GTAC_w
Cuts at: 0 791 1177 1305 1383
Size: 791 386 128 78
Fragments arranged by size:
791 386 128 78
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 259 1383
Size: 259 1124
XmnI GAAnn'nnTTC
Cuts at: 0 691 775 1111 1148 1195 1279 1316 1383
Size: 691 84 336 37 47 84 37 67
Fragments arranged by size:
691 336 84 84 67 47 37 37
Enzymes that do cut:
AccI AcuI AflIII ApoI BamHI BbsI BmgBI BmrI
BpmI BpuEI BsaAI BsaWI BseRI BsmI BsrDI BsrGI
BssSI BstBI BstYI BstZ17I BtgZI BtsI CspCI Eco57MI
EcoO109I EcoRI Hin4I HincII HindIII HpaI NspI PciI
PfoI PpuMI PsiI SfcI SmlI TatI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BanI BanII BbeI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmtI BplI Bpu10I BsaI BsaBI BsaHI BsaXI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrFI BssHII BstAPI BstEII BstXI Bsu36I
BtgI ClaI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI EcoNI EcoRV FalI FseI FspI FspAI
HaeII KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PflMI PmeI PmlI PpiI
PshAI PspOMI PspXI PsrI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StuI StyI SwaI TaqII TstI Tth111I
XbaI XhoI XmaI ZraI