(Circular) (Six-base) MAPSORT of: pFN21AB5974.seq Check: 1901 from: 1 to: 6385 pFN21AB5974 6385 bp With 182 enzymes: SgfI * April 15, 2010 09:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4195 278 Size: 53 83 186 936 2659 2468 Fragments arranged by size: 2659 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3185 3728 1937 Size: 1248 543 4594 Fragments arranged by size: 4594 1248 543 Acc65I G'GTAC_C Cuts at: 3706 3706 Size: 6385 AclI AA'CG_TT Cuts at: 1563 4512 4885 1563 Size: 2949 373 3063 Fragments arranged by size: 3063 2949 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2066 2292 2308 2998 3099 4448 5832 6100 Size: 434 226 16 690 101 1349 1384 268 Cuts at: 6100 1632 Size: 1917 Fragments arranged by size: 1917 1384 1349 690 434 268 226 101 16 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6188 AflIII A'CryG_T Cuts at: 1203 5284 6261 1203 Size: 4081 977 1327 Fragments arranged by size: 4081 1327 977 AgeI A'CCGG_T Cuts at: 5216 5216 Size: 6385 AhdI GACnn_n'nnGTC Cuts at: 5114 5114 Size: 6385 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6385 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6385 AlwNI CAG_nnn'CTG Cuts at: 3602 4128 5700 3602 Size: 526 1572 4287 Fragments arranged by size: 4287 1572 526 ApaLI G'TGCA_C Cuts at: 3633 4442 5598 3633 Size: 809 1156 4420 Fragments arranged by size: 4420 1156 809 ApoI r'AATT_y Cuts at: 1493 2240 2704 3694 3920 4072 6181 1493 Size: 747 464 990 226 152 2109 1697 Fragments arranged by size: 2109 1697 990 747 464 226 152 AseI AT'TA_AT Cuts at: 160 1032 4939 160 Size: 872 3907 1606 Fragments arranged by size: 3907 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6385 AvaI C'yCGr_G Cuts at: 1870 1943 3710 1870 Size: 73 1767 4545 Fragments arranged by size: 4545 1767 73 BamHI G'GATC_C Cuts at: 2329 3715 4082 2329 Size: 1386 367 4632 Fragments arranged by size: 4632 1386 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3706 5161 618 Size: 325 217 627 1919 1455 1842 Fragments arranged by size: 1919 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3704 1391 Size: 2313 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3440 928 Size: 971 1541 3873 Fragments arranged by size: 3873 1541 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4576 4610 1903 Size: 34 2639 34 3678 Fragments arranged by size: 3678 2639 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4277 5487 4277 Size: 1210 5175 BclI T'GATC_A Cuts at: 1548 3034 1548 Size: 1486 4899 BfrBI ATG'CAT Cuts at: 2426 2738 2426 Size: 312 6073 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4996 136 Size: 107 122 71 631 605 337 2987 1525 Fragments arranged by size: 2987 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 2303 6380 2303 Size: 4077 2308 BlpI GC'TnA_GC Cuts at: 3802 3802 Size: 6385 Bme1580I G_kGCm'C Cuts at: 1790 2534 3637 4446 5602 1790 Size: 744 1103 809 1156 2573 Fragments arranged by size: 2573 1156 1103 809 744 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6385 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2543 3090 4116 Size: 416 53 83 399 24 1126 547 1026 Cuts at: 4116 5074 442 Size: 958 1753 Fragments arranged by size: 1753 1126 1026 958 547 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3207 1056 Size: 2151 4234 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2619 2863 3157 3624 5045 1239 Size: 1380 244 294 467 1421 2579 Fragments arranged by size: 2579 1421 1380 467 294 244 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2550 4510 5375 5673 2550 Size: 1960 865 298 3262 Fragments arranged by size: 3262 1960 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5048 882 Size: 617 3549 2219 Fragments arranged by size: 3549 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4176 493 Size: 711 2972 2702 Fragments arranged by size: 2972 2702 711 BsaBI GATnn'nnATC Cuts at: 6379 6379 Size: 6385 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4192 4574 5209 275 Size: 53 83 186 936 2659 382 635 1451 Fragments arranged by size: 2659 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4818 5216 5490 5637 6048 4818 Size: 398 274 147 411 5155 Fragments arranged by size: 5155 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3421 3451 1178 Size: 30 165 30 2018 30 4112 Fragments arranged by size: 4112 2018 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2048 2631 3387 1186 Size: 862 583 756 4184 Fragments arranged by size: 4184 862 756 583 BseYI C'CCAG_C Cuts at: 5588 6084 5588 Size: 496 5889 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2490 2501 3228 1163 Size: 573 754 11 727 4320 Fragments arranged by size: 4320 754 727 573 11 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2136 3835 4596 5624 1540 Size: 275 186 135 1699 761 1028 2301 Fragments arranged by size: 2301 1699 1028 761 275 186 135 BsiHKAI G_wGCw'C Cuts at: 3637 4446 4531 5602 3637 Size: 809 85 1071 4420 Fragments arranged by size: 4420 1071 809 85 BsmI GAATG_Cn' Cuts at: 3896 3989 3896 Size: 93 6292 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2534 3637 3704 4446 4531 5602 1391 Size: 399 744 1103 67 742 85 1071 2174 Fragments arranged by size: 2174 1103 1071 744 742 399 85 67 BspEI T'CCGG_A Cuts at: 6048 6048 Size: 6385 BspHI T'CATG_A Cuts at: 4274 6004 4274 Size: 1730 4655 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3740 844 Size: 2896 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4272 1394 Size: 429 2449 3507 Fragments arranged by size: 3507 2449 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3217 4880 5054 65 Size: 1183 1969 1663 174 1396 Fragments arranged by size: 1969 1663 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2066 5029 5216 1700 Size: 110 256 2963 187 2869 Fragments arranged by size: 2963 2869 256 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6385 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6385 BssSI C'ACGA_G Cuts at: 4445 5457 4445 Size: 1012 5373 BstAPI GCAn_nnn'nTGC Cuts at: 794 2723 2993 794 Size: 1929 270 4186 Fragments arranged by size: 4186 1929 270 BstBI TT'CG_AA Cuts at: 5189 5189 Size: 6385 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6385 BstXI CCAn_nnnn'nTGG Cuts at: 2410 2410 Size: 6385 BstYI r'GATC_y Cuts at: 1972 2303 2329 3715 4082 4467 4484 5936 6022 Size: 331 26 1386 367 385 17 1452 86 Cuts at: 6022 6034 6380 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1386 385 367 346 331 86 26 17 12 BstZ17I GTA'TAC Cuts at: 3186 3186 Size: 6385 Bsu36I CC'TnA_GG Cuts at: 2469 2469 Size: 6385 BtgI C'CryG_G Cuts at: 513 1065 2004 3417 513 Size: 552 939 1413 3481 Fragments arranged by size: 3481 1413 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5646 Fragments arranged by size: 5646 683 56 BtsI GCAGTG_nn' Cuts at: 2513 3396 3901 4695 4715 2513 Size: 883 505 794 20 4183 Fragments arranged by size: 4183 883 794 505 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6385 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6350 DraI TTT'AAA Cuts at: 3689 3689 Size: 6385 DraIII CAC_nnn'GTG Cuts at: 2197 2197 Size: 6385 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5392 809 Size: 507 4076 1802 Fragments arranged by size: 4076 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2010 3832 4721 Size: 54 1425 50 275 18 180 1822 889 Cuts at: 4721 6350 8 Size: 1629 43 Fragments arranged by size: 1822 1629 1425 889 275 180 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3832 1537 Size: 275 2020 4090 Fragments arranged by size: 4090 2020 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4315 1353 Size: 2962 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4972 5346 5492 387 Size: 1064 138 3383 374 146 1280 Fragments arranged by size: 3383 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2066 2292 2308 2619 2863 2998 3099 Size: 393 434 226 16 311 244 135 101 Cuts at: 3099 3157 3624 4448 5045 5832 6100 1239 Size: 58 467 824 597 787 268 1524 Fragments arranged by size: 1524 824 787 597 467 434 393 311 268 244 226 135 101 58 16 EcoO109I rG'GnC_Cy Cuts at: 2439 2439 Size: 6385 EcoRI G'AATT_C Cuts at: 2240 2704 3694 4072 2240 Size: 464 990 378 4553 Fragments arranged by size: 4553 990 464 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3129 3129 Size: 6385 FspI TGC'GCA Cuts at: 4891 4891 Size: 6385 HaeII r_GCGC'y Cuts at: 1678 5532 1678 Size: 3854 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2288 2320 2370 2402 2600 Size: 32 135 32 716 32 50 32 198 Cuts at: 2600 2632 5026 5058 5100 5132 1373 Size: 32 2394 32 42 32 2626 Fragments arranged by size: 2626 2394 716 198 135 50 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2125 2649 3729 3975 669 Size: 988 281 187 524 1080 246 3079 Fragments arranged by size: 3079 1080 988 524 281 246 187 HindIII A'AGCT_T Cuts at: 748 748 Size: 6385 HpaI GTT'AAC Cuts at: 2125 3975 2125 Size: 1850 4535 KpnI G_GTAC'C Cuts at: 3710 3710 Size: 6385 MfeI C'AATT_G Cuts at: 3984 3984 Size: 6385 MluI A'CGCG_T Cuts at: 6261 6261 Size: 6385 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3677 5499 5683 1305 Size: 2372 1822 184 2007 Fragments arranged by size: 2372 2007 1822 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4563 Fragments arranged by size: 4563 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2259 3410 4343 4702 4861 518 Size: 648 1093 1151 933 359 159 2042 Fragments arranged by size: 2042 1151 1093 933 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2933 3383 3599 3801 4478 5626 5871 1606 Size: 1327 450 216 202 677 1148 245 2120 Fragments arranged by size: 2120 1327 1148 677 450 245 216 202 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4894 Fragments arranged by size: 4894 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6385 NheI G'CTAG_C Cuts at: 1052 3203 1052 Size: 2151 4234 NotI GC'GGCC_GC Cuts at: 3832 3832 Size: 6385 NsiI A_TGCA'T Cuts at: 2428 2740 2428 Size: 312 6073 NspI r_CATG'y Cuts at: 1132 1229 3415 3743 5288 1132 Size: 97 2186 328 1545 2229 Fragments arranged by size: 2229 2186 1545 328 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6385 PciI A'CATG_T Cuts at: 5284 5284 Size: 6385 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6385 PmeI GTTT'AAAC Cuts at: 3689 3689 Size: 6385 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4454 4486 5967 5999 4454 Size: 32 1481 32 4840 Fragments arranged by size: 4840 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2439 2439 Size: 6385 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6385 PsiI TTA'TAA Cuts at: 3955 3955 Size: 6385 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2887 2919 2887 Size: 32 6353 PstI C_TGCA'G Cuts at: 830 2977 3166 3737 830 Size: 2147 189 571 3478 Fragments arranged by size: 3478 2147 571 189 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6385 PvuII CAG'CTG Cuts at: 2933 3599 2933 Size: 666 5719 SalI G'TCGA_C Cuts at: 1936 3727 1936 Size: 1791 4594 SbfI CC_TGCA'GG Cuts at: 3737 3737 Size: 6385 ScaI AGT'ACT Cuts at: 4633 6345 4633 Size: 1712 4673 SfcI C'TryA_G Cuts at: 826 1045 2655 2814 2973 3162 3442 3733 4868 Size: 219 1610 159 159 189 280 291 1135 Cuts at: 4868 5549 5740 826 Size: 681 191 1471 Fragments arranged by size: 1610 1471 1135 681 291 280 219 191 189 159 159 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6385 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6385 SmaI CCC'GGG Cuts at: 1872 3712 1872 Size: 1840 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2565 4489 5390 5652 820 Size: 197 926 622 1924 901 262 1553 Fragments arranged by size: 1924 1553 926 901 622 262 197 SnaBI TAC'GTA Cuts at: 493 4176 493 Size: 3683 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6385 SphI G_CATG'C Cuts at: 1132 3415 3743 1132 Size: 2283 328 3774 Fragments arranged by size: 3774 2283 328 SspI AAT'ATT Cuts at: 5 52 4309 5 Size: 47 4257 2081 Fragments arranged by size: 4257 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6385 StyI C'CwwG_G Cuts at: 513 1065 2004 3824 513 Size: 552 939 1820 3074 Fragments arranged by size: 3074 1820 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4412 4429 4582 4767 5212 325 Size: 1255 2832 17 153 185 445 1498 Fragments arranged by size: 2832 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4631 6343 96 Size: 274 80 33 51 1443 2654 1712 138 Fragments arranged by size: 2654 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3421 3453 6345 6377 3421 Size: 32 2892 32 3429 Fragments arranged by size: 3429 2892 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6385 XbaI T'CTAG_A Cuts at: 3721 3721 Size: 6385 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2816 2816 Size: 6385 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6385 XmaI C'CCGG_G Cuts at: 1870 3710 1870 Size: 1840 4545 XmnI GAAnn'nnTTC Cuts at: 4514 4514 Size: 6385 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4193 276 Size: 53 83 186 936 2659 2468 Fragments arranged by size: 2659 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI