(Circular) (Six-base) MAPSORT of: pFN21AB5973.seq Check: 982 from: 1 to: 6262 pFN21AB5973 6262 bp With 182 enzymes: SgfI * April 15, 2010 09:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4072 278 Size: 53 83 186 936 2536 2468 Fragments arranged by size: 2536 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2145 2157 3605 1937 Size: 208 12 1448 4594 Fragments arranged by size: 4594 1448 208 12 Acc65I G'GTAC_C Cuts at: 3583 3583 Size: 6262 AclI AA'CG_TT Cuts at: 1563 4389 4762 1563 Size: 2826 373 3063 Fragments arranged by size: 3063 2826 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2849 4325 5709 5977 1632 Size: 1217 1476 1384 268 1917 Fragments arranged by size: 1917 1476 1384 1217 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6065 AflIII A'CryG_T Cuts at: 1203 5161 6138 1203 Size: 3958 977 1327 Fragments arranged by size: 3958 1327 977 AgeI A'CCGG_T Cuts at: 5093 5093 Size: 6262 AhdI GACnn_n'nnGTC Cuts at: 4991 4991 Size: 6262 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6262 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6262 AlwNI CAG_nnn'CTG Cuts at: 2360 3315 4005 5577 2360 Size: 955 690 1572 3045 Fragments arranged by size: 3045 1572 955 690 ApaI G_GGCC'C Cuts at: 2121 2974 2121 Size: 853 5409 ApaLI G'TGCA_C Cuts at: 4319 5475 4319 Size: 1156 5106 ApoI r'AATT_y Cuts at: 1493 3571 3797 3949 6058 1493 Size: 2078 226 152 2109 1697 Fragments arranged by size: 2109 2078 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4816 160 Size: 872 3784 1606 Fragments arranged by size: 3784 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6262 AvaI C'yCGr_G Cuts at: 1870 1943 3404 3587 1870 Size: 73 1461 183 4545 Fragments arranged by size: 4545 1461 183 73 BamHI G'GATC_C Cuts at: 3592 3959 3592 Size: 367 5895 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2137 2350 2598 3269 3282 Size: 325 217 627 350 213 248 671 13 Cuts at: 3282 3389 3506 3583 5038 618 Size: 107 117 77 1455 1842 Fragments arranged by size: 1842 1455 671 627 350 325 248 217 213 117 107 77 13 BanII G_rGCy'C Cuts at: 1391 2121 2196 2718 2729 2745 2974 3581 1391 Size: 730 75 522 11 16 229 607 4072 Fragments arranged by size: 4072 730 607 522 229 75 16 11 BbeI G_GCGC'C Cuts at: 2141 2602 3273 3393 3510 2141 Size: 461 671 120 117 4893 Fragments arranged by size: 4893 671 461 120 117 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3322 928 Size: 971 1423 3868 Fragments arranged by size: 3868 1423 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4453 4487 1903 Size: 34 2516 34 3678 Fragments arranged by size: 3678 2516 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2018 4154 5364 2018 Size: 2136 1210 2916 Fragments arranged by size: 2916 2136 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6262 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2129 3215 4873 Size: 107 122 71 631 605 457 1086 1658 Cuts at: 4873 136 Size: 1525 Fragments arranged by size: 1658 1525 1086 631 605 457 122 107 71 BglII A'GATC_T Cuts at: 6257 6257 Size: 6262 BlpI GC'TnA_GC Cuts at: 3096 3679 3096 Size: 583 5679 Bme1580I G_kGCm'C Cuts at: 1790 2121 2974 3486 4323 5479 1790 Size: 331 853 512 837 1156 2573 Fragments arranged by size: 2573 1156 853 837 512 331 BmgBI CAC'GTC Cuts at: 1318 2625 3240 1318 Size: 1307 615 4340 Fragments arranged by size: 4340 1307 615 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2041 3325 3993 Size: 416 53 83 399 24 624 1284 668 Cuts at: 3993 4951 442 Size: 958 1753 Fragments arranged by size: 1753 1284 958 668 624 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6262 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2215 2215 Size: 6262 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2789 2882 2984 3110 4922 1239 Size: 1550 93 102 126 1812 2579 Fragments arranged by size: 2579 1812 1550 126 102 93 Bpu10I CC'TnA_GC Cuts at: 2072 3158 2072 Size: 1086 5176 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2459 2588 2705 3173 4387 5252 5550 2459 Size: 129 117 468 1214 865 298 3171 Fragments arranged by size: 3171 1214 865 468 298 129 117 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2453 2575 4925 882 Size: 617 954 122 2350 2219 Fragments arranged by size: 2350 2219 954 617 122 BsaAI yAC'GTr Cuts at: 493 1204 2079 2263 4053 493 Size: 711 875 184 1790 2702 Fragments arranged by size: 2702 1790 875 711 184 BsaBI GATnn'nnATC Cuts at: 6256 6256 Size: 6262 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2138 2217 2282 2599 Size: 53 83 186 936 605 79 65 317 Cuts at: 2599 3077 3270 3390 3492 3507 4069 4451 5086 Size: 478 193 120 102 15 562 382 635 Cuts at: 5086 275 Size: 1451 Fragments arranged by size: 1451 936 635 605 562 478 382 317 193 186 120 102 83 79 65 53 15 BsaWI w'CCGG_w Cuts at: 2090 2916 4695 5093 5367 5514 5925 2090 Size: 826 1779 398 274 147 411 2427 Fragments arranged by size: 2427 1779 826 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2018 2048 3275 3305 1178 Size: 30 165 30 615 30 1227 30 4135 Fragments arranged by size: 4135 1227 615 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2026 2693 2696 2699 2711 2714 2726 2771 Size: 840 667 3 3 12 3 12 45 Cuts at: 2771 1186 Size: 4677 Fragments arranged by size: 4677 840 667 45 12 12 3 3 3 BseYI C'CCAG_C Cuts at: 2212 3055 3424 3496 5465 5961 2212 Size: 843 369 72 1969 496 2513 Fragments arranged by size: 2513 1969 843 496 369 72 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3435 1163 Size: 573 1699 3990 Fragments arranged by size: 3990 1699 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2205 3000 3712 4473 5501 1540 Size: 275 186 204 795 712 761 1028 2301 Fragments arranged by size: 2301 1028 795 761 712 275 204 186 BsiHKAI G_wGCw'C Cuts at: 2078 2196 2496 2729 4323 4408 5479 2078 Size: 118 300 233 1594 85 1071 2861 Fragments arranged by size: 2861 1594 1071 300 233 118 85 BsmI GAATG_Cn' Cuts at: 3773 3866 3773 Size: 93 6169 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2078 2121 2196 2496 2718 2729 2745 Size: 399 288 43 75 300 222 11 16 Cuts at: 2745 2974 3486 3581 4323 4408 5479 1391 Size: 229 512 95 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 512 399 300 288 229 222 95 85 75 43 16 11 BspEI T'CCGG_A Cuts at: 2090 2916 5925 2090 Size: 826 3009 2427 Fragments arranged by size: 3009 2427 826 BspHI T'CATG_A Cuts at: 4151 5881 4151 Size: 1730 4532 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3617 844 Size: 2773 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4149 1394 Size: 429 2326 3507 Fragments arranged by size: 3507 2326 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4757 4931 65 Size: 1183 3509 174 1396 Fragments arranged by size: 3509 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2140 2166 2595 2815 3067 3539 4906 Size: 110 330 26 429 220 252 472 1367 Cuts at: 4906 5093 1700 Size: 187 2869 Fragments arranged by size: 2869 1367 472 429 330 252 220 187 110 26 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6262 BssHII G'CGCG_C Cuts at: 1925 2183 2591 2910 1925 Size: 258 408 319 5277 Fragments arranged by size: 5277 408 319 258 BssSI C'ACGA_G Cuts at: 2189 4322 5334 2189 Size: 2133 1012 3117 Fragments arranged by size: 3117 2133 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2082 794 Size: 1288 4974 BstBI TT'CG_AA Cuts at: 5066 5066 Size: 6262 BstEII G'GTnAC_C Cuts at: 1184 3298 1184 Size: 2114 4148 BstYI r'GATC_y Cuts at: 1972 2330 3592 3959 4344 4361 5813 5899 5911 Size: 358 1262 367 385 17 1452 86 12 Cuts at: 5911 6257 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1262 385 367 358 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2905 3036 3347 2905 Size: 131 311 5820 Fragments arranged by size: 5820 311 131 BtgI C'CryG_G Cuts at: 513 1065 2205 2421 2633 2745 2931 3204 513 Size: 552 1140 216 212 112 186 273 3571 Fragments arranged by size: 3571 1140 552 273 216 212 186 112 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5523 Fragments arranged by size: 5523 683 56 BtsI GCAGTG_nn' Cuts at: 3011 3778 4572 4592 3011 Size: 767 794 20 4681 Fragments arranged by size: 4681 794 767 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6262 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6227 DraI TTT'AAA Cuts at: 3566 3566 Size: 6262 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2891 5269 809 Size: 507 1575 2378 1802 Fragments arranged by size: 2378 1802 1575 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2058 2175 2202 Size: 54 1425 50 275 18 228 117 27 Cuts at: 2202 2538 2627 2928 2997 3709 4598 6227 8 Size: 336 89 301 69 712 889 1629 43 Fragments arranged by size: 1629 1425 889 712 336 301 275 228 117 89 69 54 50 43 27 18 EagI C'GGCC_G Cuts at: 1537 1812 2202 2997 3709 1537 Size: 275 390 795 712 4090 Fragments arranged by size: 4090 795 712 390 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4192 1353 Size: 2839 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2123 2253 2275 2955 3462 4849 Size: 1064 138 534 130 22 680 507 1387 Cuts at: 4849 5223 5369 387 Size: 374 146 1280 Fragments arranged by size: 1387 1280 1064 680 534 507 374 146 138 130 22 EcoICRI GAG'CTC Cuts at: 2194 2727 2194 Size: 533 5729 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2789 2849 2882 2984 3110 4325 4922 Size: 393 1157 60 33 102 126 1215 597 Cuts at: 4922 5709 5977 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1215 1157 787 597 393 268 126 102 60 33 EcoNI CCTnn'n_nnAGG Cuts at: 2407 2564 2606 3533 2407 Size: 157 42 927 5136 Fragments arranged by size: 5136 927 157 42 EcoO109I rG'GnC_Cy Cuts at: 2228 2344 2959 2970 3200 2228 Size: 116 615 11 230 5290 Fragments arranged by size: 5290 615 230 116 11 EcoRI G'AATT_C Cuts at: 3571 3949 3571 Size: 378 5884 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2854 2854 Size: 6262 FspI TGC'GCA Cuts at: 2337 2647 4768 2337 Size: 310 2121 3831 Fragments arranged by size: 3831 2121 310 HaeII r_GCGC'y Cuts at: 1678 2141 2213 2602 3196 3273 3393 3510 5409 Size: 463 72 389 594 77 120 117 1899 Cuts at: 5409 1678 Size: 2531 Fragments arranged by size: 2531 1899 594 463 389 120 117 77 72 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3161 3193 4903 4935 4977 Size: 32 135 32 1589 32 1710 32 42 Cuts at: 4977 5009 1373 Size: 32 2626 Fragments arranged by size: 2626 1710 1589 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3606 3852 669 Size: 988 281 1668 246 3079 Fragments arranged by size: 3079 1668 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6262 HpaI GTT'AAC Cuts at: 3852 3852 Size: 6262 KasI G'GCGC_C Cuts at: 2137 2598 3269 3389 3506 2137 Size: 461 671 120 117 4893 Fragments arranged by size: 4893 671 461 120 117 KpnI G_GTAC'C Cuts at: 3587 3587 Size: 6262 MfeI C'AATT_G Cuts at: 3861 3861 Size: 6262 MluI A'CGCG_T Cuts at: 6138 6138 Size: 6262 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5376 5560 1305 Size: 4071 184 2007 Fragments arranged by size: 4071 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2060 2177 2540 10 Size: 54 1425 343 228 117 363 3732 Fragments arranged by size: 3732 1425 363 343 228 117 54 MslI CAynn'nnrTG Cuts at: 518 1166 2552 4220 4579 4738 518 Size: 648 1386 1668 359 159 2042 Fragments arranged by size: 2042 1668 1386 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2207 2360 2430 2635 2800 2933 3137 3206 Size: 601 153 70 205 165 133 204 69 Cuts at: 3206 3500 3678 4355 5503 5748 1606 Size: 294 178 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 601 294 245 205 204 178 165 153 133 70 69 NaeI GCC'GGC Cuts at: 2168 2597 2817 3069 3541 2168 Size: 429 220 252 472 4889 Fragments arranged by size: 4889 472 429 252 220 NarI GG'CG_CC Cuts at: 2138 2599 3270 3390 3507 2138 Size: 461 671 120 117 4893 Fragments arranged by size: 4893 671 461 120 117 NcoI C'CATG_G Cuts at: 513 1065 2421 513 Size: 552 1356 4354 Fragments arranged by size: 4354 1356 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6262 NgoMIV G'CCGG_C Cuts at: 2166 2595 2815 3067 3539 2166 Size: 429 220 252 472 4889 Fragments arranged by size: 4889 472 429 252 220 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6262 NotI GC'GGCC_GC Cuts at: 3709 3709 Size: 6262 NspI r_CATG'y Cuts at: 1132 1229 3187 3620 5165 1132 Size: 97 1958 433 1545 2229 Fragments arranged by size: 2229 1958 1545 433 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6262 PciI A'CATG_T Cuts at: 5161 5161 Size: 6262 PflMI CCAn_nnn'nTGG Cuts at: 1108 2848 1108 Size: 1740 4522 PfoI T'CCnGG_A Cuts at: 3396 3396 Size: 6262 PmeI GTTT'AAAC Cuts at: 3566 3566 Size: 6262 PmlI CAC'GTG Cuts at: 2079 2079 Size: 6262 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4331 4363 5844 5876 4331 Size: 32 1481 32 4717 Fragments arranged by size: 4717 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2228 2959 3200 2228 Size: 731 241 5290 Fragments arranged by size: 5290 731 241 PshAI GACnn'nnGTC Cuts at: 1613 2856 1613 Size: 1243 5019 PsiI TTA'TAA Cuts at: 3832 3832 Size: 6262 PspOMI G'GGCC_C Cuts at: 2117 2970 2117 Size: 853 5409 PstI C_TGCA'G Cuts at: 830 2490 3505 3614 830 Size: 1660 1015 109 3478 Fragments arranged by size: 3478 1660 1015 109 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6262 PvuII CAG'CTG Cuts at: 3500 3500 Size: 6262 SacI G_AGCT'C Cuts at: 2196 2729 2196 Size: 533 5729 SacII CC_GC'GG Cuts at: 2208 2636 2934 3207 2208 Size: 428 298 273 5263 Fragments arranged by size: 5263 428 298 273 SalI G'TCGA_C Cuts at: 1936 3604 1936 Size: 1668 4594 SanDI GG'GwC_CC Cuts at: 2228 2228 Size: 6262 SbfI CC_TGCA'GG Cuts at: 3614 3614 Size: 6262 ScaI AGT'ACT Cuts at: 4510 6222 4510 Size: 1712 4550 SexAI A'CCwGG_T Cuts at: 2576 2576 Size: 6262 SfcI C'TryA_G Cuts at: 826 1045 2486 3501 3610 4745 5426 5617 826 Size: 219 1441 1015 109 1135 681 191 1471 Fragments arranged by size: 1471 1441 1135 1015 681 219 191 109 SfoI GGC'GCC Cuts at: 2139 2600 3271 3391 3508 2139 Size: 461 671 120 117 4893 Fragments arranged by size: 4893 671 461 120 117 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6262 SgrAI Cr'CCGG_yG Cuts at: 1700 2595 2815 1700 Size: 895 220 5147 Fragments arranged by size: 5147 895 220 SmaI CCC'GGG Cuts at: 1872 3406 3589 1872 Size: 1534 183 4545 Fragments arranged by size: 4545 1534 183 SmlI C'TyrA_G Cuts at: 820 1017 1943 2474 2603 2720 3188 4366 5267 Size: 197 926 531 129 117 468 1178 901 Cuts at: 5267 5529 820 Size: 262 1553 Fragments arranged by size: 1553 1178 926 901 531 468 262 197 129 117 SnaBI TAC'GTA Cuts at: 493 4053 493 Size: 3560 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6262 SphI G_CATG'C Cuts at: 1132 3187 3620 1132 Size: 2055 433 3774 Fragments arranged by size: 3774 2055 433 SspI AAT'ATT Cuts at: 5 52 4186 5 Size: 47 4134 2081 Fragments arranged by size: 4134 2081 47 StuI AGG'CCT Cuts at: 1517 2480 2903 3087 3371 1517 Size: 963 423 184 284 4408 Fragments arranged by size: 4408 963 423 284 184 StyI C'CwwG_G Cuts at: 513 1065 2316 2421 2517 3196 3384 3534 3701 Size: 552 1251 105 96 679 188 150 167 Cuts at: 3701 513 Size: 3074 Fragments arranged by size: 3074 1251 679 552 188 167 150 105 96 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3307 4289 4306 4459 4644 5089 325 Size: 1255 1727 982 17 153 185 445 1498 Fragments arranged by size: 1727 1498 1255 982 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4508 6220 96 Size: 274 80 33 51 1443 2531 1712 138 Fragments arranged by size: 2531 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3275 3307 6222 6254 3275 Size: 32 2915 32 3283 Fragments arranged by size: 3283 2915 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3326 1316 Size: 2010 4252 XbaI T'CTAG_A Cuts at: 3598 3598 Size: 6262 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6262 XmaI C'CCGG_G Cuts at: 1870 3404 3587 1870 Size: 1534 183 4545 Fragments arranged by size: 4545 1534 183 XmnI GAAnn'nnTTC Cuts at: 4391 4391 Size: 6262 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4070 276 Size: 53 83 186 936 2536 2468 Fragments arranged by size: 2536 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I DraIII EcoRV FseI FspAI NruI NsiI PacI PspXI PsrI RsrII SapI SfiI SrfI SwaI XcmI