Restriction map for
pFN21AB5972
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# NO CUT ENZYMES ( 166 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BalI BamHI BanI BanII BbeI
BbrPI BbvCI BcgI BciVI BcnI
BfaI BfrI BglI BglII BlnI
BlpI BmyI Bpu1102I BsaAI BsaBI
BsaHI BseAI BsgI BsiEI BsiHKAI
BsiWI BsmBI BsmFI BsoBI Bsp1286I
BspDI BspEI BspLU11I BsrBI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstYI
BstZ17I Bsu36I Cac8I CelII CfoI
Cfr10I ClaI CpoI DraI DraIII
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRV FauI FseI FspI HaeII
HaeIII HgaI HhaI Hin1I HinP1I
HpaI KasI KpnI KspI MaeI
MaeII MamI MfeI MflI MluI
MluNI MroI MscI MspA1I MunI
MvnI MwoI NaeI NarI NciI
NgoMIV NheI NotI NruI NspV
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PstI
PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I StuI
SwaI TaqI Tth111I TthHB8I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AlwI 16 GGATC GGATC(4/5)
BbvI 66 GCAGC GCAGC(8/12)
BclI 2 TGATCA T^GATCA
BpmI 112 CTGGAG CTGGAG(16/14)
BsaI 972 GAGACC GGTCTC(1/5)
BsaWI 1354 TCCGGT [AT]^CCGG[AT]
BspMI 517 ACCTGC ACCTGC(4/8)
BsrDI 1530 CATTGC GCAATG(2/0)
BsrI 251 CCAGT ACTGG(1/-1)
BssKI 12 CCAGG ^CC[ACGT]GG
BstNI 12 CCAGG CC^[AT]GG
DraII 88 AGGACCC [GA]G^G[ACGT]CC[CT]
DsaI 196 CCATGG C^C[GA][CT]GG
EcoO109I 88 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoRII 12 CCAGG ^CC[AT]GG
EcoT14I 196 CCATGG C^C[AT][AT]GG
EcoT22I 1157 ATGCAT ATGCA^T
FbaI 2 TGATCA T^GATCA
Fnu4HI 66 GCAGC GC^[ACGT]GC
HapII 1355 CCGG C^CGG
HindIII 458 AAGCTT A^AGCTT
HpaII 1355 CCGG C^CGG
ItaI 66 GCAGC GC^[ACGT]GC
MspI 1355 CCGG C^CGG
MvaI 12 CCAGG CC^[AT]GG
NcoI 196 CCATGG C^CATGG
NdeI 1061 CATATG CA^TATG
NsiI 1157 ATGCAT ATGCA^T
NspI 1159 GCATGT [GA]CATG^[CT]
PacI 241 TTAATTAA TTAAT^TAA
PleI 228 GAGTC GAGTC(4/5)
PpuMI 88 AGGACCC [GA]G^G[AT]CC[CT]
ScrFI 12 CCAGG CC^[ACGT]GG
SfcI 120 CTACAG C^T[GA][CT]AG
SspI 721 AATATT AAT^ATT
StyI 196 CCATGG C^C[AT][AT]GG
TseI 66 GCAGC G^C[AT]GC
Tsp45I 37 GTGAC ^GT[GC]AC
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# TWO CUT ENZYMES ( 24 enzymes) #
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AluI 376 AGCT AG^CT
459 AGCT
AlwNI 67 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG
375 CAGCTTCTG
AseI 240 ATTAAT AT^TAAT
244 ATTAAT
AsnI 240 ATTAAT AT^TAAT
244 ATTAAT
AvaII 75 GGACC G^G[AT]CC
89 GGACC
BseRI 58 GAGGAG GAGGAG(10/8)
190 GAGGAG
BsiYI 92 CCCTGTACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
93 CCTGTACAGGG
BslI 92 CCCTGTACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
93 CCTGTACAGGG
BsmAI 972 GAGAC GTCTC(1/5)
133 GTCTC
BsmI 1150 GAATGC GAATGC(1/-1)
149 GCATTC
Cfr13I 75 GGACC G^G[ACGT]CC
89 GGACC
DpnII 3 GATC ^GATC
17 GATC
EarI 478 CTCTTC CTCTTC(1/4)
340 GAAGAG
HincII 1017 GTCAAC GT[CT]^[GA]AC
1269 GTCAAC
HindII 1017 GTCAAC GT[CT]^[GA]AC
1269 GTCAAC
Ksp632I 478 CTCTTC CTCTTC(1/4)
340 GAAGAG
MaeIII 37 GTGAC ^GT[ACGT]AC
500 GTTAC
MboI 3 GATC ^GATC
17 GATC
NdeII 3 GATC ^GATC
17 GATC
PshBI 240 ATTAAT AT^TAAT
244 ATTAAT
Sau3AI 3 GATC ^GATC
17 GATC
Sau96I 75 GGACC G^G[ACGT]CC
89 GGACC
SmlI 53 CTCAAG C^T[CT][GA]AG
172 CTCAAG
TfiI 168 GATTC G^A[AT]TC
634 GAATC
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# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
#########################################
AcsI AfaI ApoI Asp700I BbsI
BpuAI BsaJI Bsp1407I BspHI BsrGI
BstF5I DdeI EcoRI FokI HinfI
HphI MboII MnlI MseI MslI
NlaIII NlaIV RcaI RsaI SfaNI
SspBI Tru9I Tsp509I TspRI XmnI