Restriction map for
pFN21AB5972
(Linear) (Six-base) MAPSORT of: KIBB5972.seq Check: 2213 from: 1 to: 1683
KIBB5972.orf
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 359 990 1683
Size: 359 631 693
Fragments arranged by size:
693 631 359
AleI CACnn'nnGTG
Cuts at: 0 1217 1301 1683
Size: 1217 84 382
Fragments arranged by size:
1217 382 84
AlwNI CAG_nnn'CTG
Cuts at: 0 72 380 1683
Size: 72 308 1303
Fragments arranged by size:
1303 308 72
ApoI r'AATT_y
Cuts at: 0 214 471 930 956 1336 1683
Size: 214 257 459 26 380 347
Fragments arranged by size:
459 380 347 257 214 26
AseI AT'TA_AT
Cuts at: 0 241 245 1683
Size: 241 4 1438
Fragments arranged by size:
1438 241 4
BbsI GAAGACnn'nnnn_
Cuts at: 0 398 922 1383 1683
Size: 398 524 461 300
Fragments arranged by size:
524 461 398 300
BclI T'GATC_A
Cuts at: 0 2 1683
Size: 2 1681
BfrBI ATG'CAT
Cuts at: 0 1159 1683
Size: 1159 524
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 133 1683
Size: 133 1550
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 38 157 1683
Size: 38 119 1526
Fragments arranged by size:
1526 119 38
BsaI GGTCTCn'nnnn_
Cuts at: 0 966 1683
Size: 966 717
BsaWI w'CCGG_w
Cuts at: 0 1354 1683
Size: 1354 329
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 73 205 1683
Size: 73 132 1478
Fragments arranged by size:
1478 132 73
BsmI GAATG_Cn'
Cuts at: 0 149 1156 1683
Size: 149 1007 527
Fragments arranged by size:
1007 527 149
BspHI T'CATG_A
Cuts at: 0 819 903 987 1071 1683
Size: 819 84 84 84 612
Fragments arranged by size:
819 612 84 84 84
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 526 1683
Size: 526 1157
BsrDI GCAATG_nn'
Cuts at: 0 1529 1683
Size: 1529 154
BsrGI T'GTAC_A
Cuts at: 0 95 486 1617 1683
Size: 95 391 1131 66
Fragments arranged by size:
1131 391 95 66
BtgI C'CryG_G
Cuts at: 0 196 1683
Size: 196 1487
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 43 78 432 467 1683
Size: 43 35 354 35 1216
Fragments arranged by size:
1216 354 43 35 35
EarI CTCTTCn'nnn_
Cuts at: 0 335 484 1683
Size: 335 149 1199
Fragments arranged by size:
1199 335 149
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 133 359 990 1683
Size: 133 226 631 693
Fragments arranged by size:
693 631 226 133
EcoO109I rG'GnC_Cy
Cuts at: 0 89 1683
Size: 89 1594
EcoRI G'AATT_C
Cuts at: 0 471 930 956 1683
Size: 471 459 26 727
Fragments arranged by size:
727 471 459 26
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 608 640 1683
Size: 608 32 1043
Fragments arranged by size:
1043 608 32
HincII GTy'rAC
Cuts at: 0 1019 1271 1683
Size: 1019 252 412
Fragments arranged by size:
1019 412 252
HindIII A'AGCT_T
Cuts at: 0 458 1683
Size: 458 1225
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 158 1683
Size: 158 1525
MslI CAynn'nnrTG
Cuts at: 0 656 824 908 992 1065 1076 1217 1301
Size: 656 168 84 84 73 11 141 84
Cuts at: 1301 1683
Size: 382
Fragments arranged by size:
656 382 168 141 84 84 84 73
11
NcoI C'CATG_G
Cuts at: 0 196 1683
Size: 196 1487
NdeI CA'TA_TG
Cuts at: 0 1062 1683
Size: 1062 621
NsiI A_TGCA'T
Cuts at: 0 1161 1683
Size: 1161 522
NspI r_CATG'y
Cuts at: 0 1163 1683
Size: 1163 520
PacI TTA_AT'TAA
Cuts at: 0 245 1683
Size: 245 1438
PasI CC'CwG_GG
Cuts at: 0 12 1683
Size: 12 1671
PpuMI rG'GwC_Cy
Cuts at: 0 89 1683
Size: 89 1594
SfcI C'TryA_G
Cuts at: 0 120 1683
Size: 120 1563
SmlI C'TyrA_G
Cuts at: 0 53 172 1683
Size: 53 119 1511
Fragments arranged by size:
1511 119 53
SspI AAT'ATT
Cuts at: 0 723 1683
Size: 723 960
StyI C'CwwG_G
Cuts at: 0 196 1683
Size: 196 1487
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 856 1683
Size: 856 827
TatI w'GTAC_w
Cuts at: 0 95 486 1617 1683
Size: 95 391 1131 66
Fragments arranged by size:
1131 391 95 66
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 531 563 1683
Size: 531 32 1120
Fragments arranged by size:
1120 531 32
XmnI GAAnn'nnTTC
Cuts at: 0 167 215 261 329 376 460 638 919
Size: 167 48 46 68 47 84 178 281
Cuts at: 919 1003 1087 1255 1322 1423 1683
Size: 84 84 168 67 101 260
Fragments arranged by size:
281 260 178 168 167 101 84 84
84 68 67 48 47 46
Enzymes that do cut:
AcuI AleI AlwNI ApoI AseI BbsI BclI BfrBI
BpmI BpuEI BsaI BsaWI BseRI BsmI BspHI BspMI
BsrDI BsrGI BtgI CspCI EarI Eco57MI EcoO109I EcoRI
Hin4I HincII HindIII MmeI MslI NcoI NdeI NsiI
NspI PacI PasI PpuMI SfcI SmlI SspI StyI
TaqII TatI TstI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AlfI AloI ApaI ApaLI AscI AsiSI
AvaI AvrII BaeI BamHI BanI BanII BbeI BbvCI
BcgI BciVI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI Bpu10I BsaAI BsaBI BsaHI BsaXI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspEI BsrBI
BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgZI BtsI ClaI DraI DraIII DrdI
EaeI EagI EciI EcoICRI EcoNI EcoRV FalI FseI
FspI FspAI HaeII HpaI KasI KpnI MfeI MluI
MscI MspA1I NaeI NarI NgoMIV NheI NotI NruI
PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
StuI SwaI Tth111I XbaI XcmI XhoI XmaI ZraI