Restriction map for
pFN21AB5967
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# NO CUT ENZYMES ( 128 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI AlwI
AocI Aor51HI AscI AseI AsnI
Asp700I Asp718I AspEI AviII AvrII
BamHI BbeI BbsI BbvCI BcgI
BciVI BfrI BglII BlnI BpuAI
BsaBI BsaHI BsaI BsaWI BseAI
BseRI BsiEI BsiWI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrFI
BsrGI BssSI Bst1107I BstAPI BstEII
BstPI BstXI BstYI BstZ17I Bsu36I
Cfr10I ClaI CpoI DraI DrdI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT22I FseI FspI HaeII Hin1I
HincII HindII HpaI KasI KpnI
Ksp632I KspI MamI MfeI MflI
MluI MroI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI PacI PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfaNI SfiI SfoI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
SspI StuI SwaI XbaI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 32 enzymes) #
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AccII 90 CGCG CG^CG
AflIII 1196 ACACGT A^C[GA][CT]GT
AspI 1107 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 1197 CACGTG CAC^GTG
BclI 221 TGATCA T^GATCA
BcnI 431 CCGGG CC^[GC]GG
BglI 427 GCCACCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 379 GCTGAGC GC^T[ACGT]AGC
BpmI 43 CTGGAG CTGGAG(16/14)
Bpu1102I 379 GCTGAGC GC^T[ACGT]AGC
BsmBI 456 CGTCTC CGTCTC(1/5)
BsmI 746 GAATGC GAATGC(1/-1)
BssHII 89 GCGCGC G^CGCGC
BstBI 75 TTCGAA TT^CGAA
BstUI 90 CGCG CG^CG
CelII 379 GCTGAGC GC^T[ACGT]AGC
EcoNI 1097 CCTCCTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 221 TGATCA T^GATCA
HindIII 1127 AAGCTT A^AGCTT
MvnI 90 CGCG CG^CG
NciI 431 CCGGG CC^[GC]GG
NspV 75 TTCGAA TT^CGAA
PaeR7I 833 CTCGAG C^TCGAG
PflFI 1107 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1197 CACGTG CAC^GTG
PmlI 1197 CACGTG CAC^GTG
PstI 1426 CTGCAG CTGCA^G
SacI 836 GAGCTC GAGCT^C
SfuI 75 TTCGAA TT^CGAA
SphI 1208 GCATGC GCATG^C
Tth111I 1107 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 833 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 26 enzymes) #
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Alw44I 409 GTGCAC G^TGCAC
1513 GTGCAC
AlwNI 1084 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG
1221 CAGCAGCTG
ApaI 98 GGGCCC GGGCC^C
1297 GGGCCC
ApaLI 409 GTGCAC G^TGCAC
1513 GTGCAC
BalI 1201 TGGCCA TGG^CCA
1325 TGGCCA
BsaAI 406 TACGTG [CT]AC^GT[GA]
1197 CACGTG
BspMI 62 ACCTGC ACCTGC(4/8)
321 ACCTGC
BsrBI 1062 CCGCTC CCGCTC(-3/-3)
1280 CCGCTC
HapII 431 CCGG C^CGG
893 CCGG
HgaI 7 GACGC GACGC(5/10)
52 GACGC
HpaII 431 CCGG C^CGG
893 CCGG
MluNI 1201 TGGCCA TGG^CCA
1325 TGGCCA
MscI 1201 TGGCCA TGG^CCA
1325 TGGCCA
MseI 281 TTAA T^TAA
673 TTAA
MslI 213 CACGGAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1236 CACCACCGTG
MspI 431 CCGG C^CGG
893 CCGG
NcoI 1250 CCATGG C^CATGG
1332 CCATGG
NspI 1133 GCATGT [GA]CATG^[CT]
1208 GCATGC
PflMI 201 CCACGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1389 CCAGCCTGTGG
PpuMI 862 GGGTCCC [GA]G^G[AT]CC[CT]
905 AGGACCT
SmlI 472 CTCAAG C^T[CT][GA]AG
833 CTCGAG
TaqI 76 TCGA T^CGA
834 TCGA
Tru9I 281 TTAA T^TAA
673 TTAA
TthHB8I 76 TCGA T^CGA
834 TCGA
Van91I 201 CCACGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1389 CCAGCCTGTGG
XcmI 482 CCAACAACCACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
873 CCAGTGTGAGAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AspHI AvaI AvaII BanI BanII
BbvI BfaI BmyI BsaJI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HhaI HinP1I HinfI
HphI ItaI MaeI MaeII MaeIII
MboI MboII MnlI MspA1I MvaI
MwoI NdeII NlaIII NlaIV PleI
PvuII RsaI Sau3AI Sau96I ScrFI
SfcI StyI TfiI TseI Tsp45I
Tsp509I TspRI