(Circular) (Six-base) MAPSORT of: pFN21AB5967.seq Check: 3230 from: 1 to: 6259 pFN21AB5967 6259 bp With 182 enzymes: SgfI * April 15, 2010 09:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4069 278 Size: 53 83 186 936 2533 2468 Fragments arranged by size: 2533 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3602 1937 Size: 1665 4594 Acc65I G'GTAC_C Cuts at: 3580 3580 Size: 6259 AclI AA'CG_TT Cuts at: 1563 4386 4759 1563 Size: 2823 373 3063 Fragments arranged by size: 3063 2823 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3434 4322 5706 5974 1632 Size: 1802 888 1384 268 1917 Fragments arranged by size: 1917 1802 1384 888 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6062 AflIII A'CryG_T Cuts at: 1203 3201 5158 6135 1203 Size: 1998 1957 977 1327 Fragments arranged by size: 1998 1957 1327 977 AgeI A'CCGG_T Cuts at: 5090 5090 Size: 6259 AhdI GACnn_n'nnGTC Cuts at: 4988 4988 Size: 6259 AleI CACnn'nnGTG Cuts at: 1166 3245 1166 Size: 2079 4180 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6259 AlwNI CAG_nnn'CTG Cuts at: 3094 3231 4002 5574 3094 Size: 137 771 1572 3779 Fragments arranged by size: 3779 1572 771 137 ApaI G_GGCC'C Cuts at: 2107 3306 2107 Size: 1199 5060 ApaLI G'TGCA_C Cuts at: 2414 3518 4316 5472 2414 Size: 1104 798 1156 3201 Fragments arranged by size: 3201 1156 1104 798 ApoI r'AATT_y Cuts at: 1493 2201 2348 2524 2961 3568 3794 3946 6055 Size: 708 147 176 437 607 226 152 2109 Cuts at: 6055 1493 Size: 1697 Fragments arranged by size: 2109 1697 708 607 437 226 176 152 147 AseI AT'TA_AT Cuts at: 160 1032 4813 160 Size: 872 3781 1606 Fragments arranged by size: 3781 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6259 AvaI C'yCGr_G Cuts at: 1870 1943 2087 2099 2838 3298 3584 1870 Size: 73 144 12 739 460 286 4545 Fragments arranged by size: 4545 739 460 286 144 73 12 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2956 2989 2956 Size: 33 6226 BamHI G'GATC_C Cuts at: 3589 3956 3589 Size: 367 5892 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3098 3443 3535 3580 5035 Size: 325 217 627 1311 345 92 45 1455 Cuts at: 5035 618 Size: 1842 Fragments arranged by size: 1842 1455 1311 627 345 325 217 92 45 BanII G_rGCy'C Cuts at: 1391 2107 2391 2845 3306 3578 1391 Size: 716 284 454 461 272 4072 Fragments arranged by size: 4072 716 461 454 284 272 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5288 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4450 4484 1903 Size: 34 2513 34 3678 Fragments arranged by size: 3678 2513 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4151 5361 4151 Size: 1210 5049 BclI T'GATC_A Cuts at: 1548 2226 1548 Size: 678 5581 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2438 4870 136 Size: 107 122 71 631 605 766 2432 1525 Fragments arranged by size: 2432 1525 766 631 605 122 107 71 BglII A'GATC_T Cuts at: 6254 6254 Size: 6259 BlpI GC'TnA_GC Cuts at: 2385 3676 2385 Size: 1291 4968 Bme1580I G_kGCm'C Cuts at: 1790 2107 2219 2418 3177 3306 3522 4320 5476 Size: 317 112 199 759 129 216 798 1156 Cuts at: 5476 1790 Size: 2573 Fragments arranged by size: 2573 1156 798 759 317 216 199 129 112 BmgBI CAC'GTC Cuts at: 1318 2617 1318 Size: 1299 4960 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2253 3039 3990 Size: 416 53 83 399 24 836 786 951 Cuts at: 3990 4948 442 Size: 958 1753 Fragments arranged by size: 1753 958 951 836 786 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6259 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2069 4919 1239 Size: 830 2850 2579 Fragments arranged by size: 2850 2579 830 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2462 4384 5249 5547 2462 Size: 1922 865 298 3174 Fragments arranged by size: 3174 1922 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4922 882 Size: 617 3423 2219 Fragments arranged by size: 3423 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2413 3204 4050 493 Size: 711 1209 791 846 2702 Fragments arranged by size: 2702 1209 846 791 711 BsaBI GATnn'nnATC Cuts at: 6253 6253 Size: 6259 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4066 4448 5083 275 Size: 53 83 186 936 2533 382 635 1451 Fragments arranged by size: 2533 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4692 5090 5364 5511 5922 4692 Size: 398 274 147 411 5029 Fragments arranged by size: 5029 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2408 2438 3326 3356 3365 Size: 30 165 30 1005 30 888 30 9 Cuts at: 3365 3395 1178 Size: 30 4042 Fragments arranged by size: 4042 1005 888 165 30 30 30 30 30 9 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6259 BseYI C'CCAG_C Cuts at: 2108 2145 3161 3176 3352 5462 5958 2108 Size: 37 1016 15 176 2110 496 2409 Fragments arranged by size: 2409 2110 1016 496 176 37 15 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2593 2720 3217 3269 1163 Size: 573 857 127 497 52 4153 Fragments arranged by size: 4153 857 573 497 127 52 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3709 4470 5498 1540 Size: 275 186 1708 761 1028 2301 Fragments arranged by size: 2301 1708 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2418 2789 2845 3522 4320 4405 5476 2418 Size: 371 56 677 798 85 1071 3201 Fragments arranged by size: 3201 1071 798 677 371 85 56 BsmI GAATG_Cn' Cuts at: 2757 3770 3863 2757 Size: 1013 93 5153 Fragments arranged by size: 5153 1013 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2467 2467 Size: 6259 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2107 2219 2391 2418 2789 2845 3177 Size: 399 317 112 172 27 371 56 332 Cuts at: 3177 3306 3522 3578 4320 4405 5476 1391 Size: 129 216 56 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 371 332 317 216 172 129 112 85 56 56 27 BspEI T'CCGG_A Cuts at: 5922 5922 Size: 6259 BspHI T'CATG_A Cuts at: 4148 5878 4148 Size: 1730 4529 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2076 2335 3614 844 Size: 1232 259 1279 3489 Fragments arranged by size: 3489 1279 1232 259 BsrBI CCG'CTC Cuts at: 1394 1823 3069 3287 4146 1394 Size: 429 1246 218 859 3507 Fragments arranged by size: 3507 1246 859 429 218 BsrDI GCAATG_nn' Cuts at: 65 1248 4754 4928 65 Size: 1183 3506 174 1396 Fragments arranged by size: 3506 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4903 5090 1700 Size: 110 3093 187 2869 Fragments arranged by size: 3093 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6259 BssHII G'CGCG_C Cuts at: 1925 2094 1925 Size: 169 6090 BssSI C'ACGA_G Cuts at: 4319 5331 4319 Size: 1012 5247 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6259 BstBI TT'CG_AA Cuts at: 2081 5063 2081 Size: 2982 3277 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6259 BstYI r'GATC_y Cuts at: 1972 3589 3956 4341 4358 5810 5896 5908 6254 Size: 1617 367 385 17 1452 86 12 346 Cuts at: 6254 1972 Size: 1977 Fragments arranged by size: 1977 1617 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2772 3255 3337 3384 513 Size: 552 939 768 483 82 47 3388 Fragments arranged by size: 3388 939 768 552 483 82 47 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5520 Fragments arranged by size: 5520 683 56 BtsI GCAGTG_nn' Cuts at: 2649 2725 2939 3416 3775 4569 4589 2649 Size: 76 214 477 359 794 20 4319 Fragments arranged by size: 4319 794 477 359 214 76 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6259 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6224 DraI TTT'AAA Cuts at: 3563 3563 Size: 6259 DraIII CAC_nnn'GTG Cuts at: 2212 2560 3047 3517 2212 Size: 348 487 470 4954 Fragments arranged by size: 4954 487 470 348 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5266 809 Size: 507 3950 1802 Fragments arranged by size: 3950 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2401 3206 Size: 54 1425 50 275 18 177 394 805 Cuts at: 3206 3330 3706 4595 6224 8 Size: 124 376 889 1629 43 Fragments arranged by size: 1629 1425 889 805 394 376 275 177 124 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3706 1537 Size: 275 1894 4090 Fragments arranged by size: 4090 1894 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4189 1353 Size: 2836 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2263 4846 5220 5366 387 Size: 1064 138 674 2583 374 146 1280 Fragments arranged by size: 2583 1280 1064 674 374 146 138 EcoICRI GAG'CTC Cuts at: 2843 2843 Size: 6259 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2069 3434 4322 4919 5706 5974 1239 Size: 393 437 1365 888 597 787 268 1524 Fragments arranged by size: 1524 1365 888 787 597 437 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 3106 3106 Size: 6259 EcoO109I rG'GnC_Cy Cuts at: 2103 2104 2868 2911 3091 3302 3303 2103 Size: 1 764 43 180 211 1 5059 Fragments arranged by size: 5059 764 211 180 43 1 1 EcoRI G'AATT_C Cuts at: 3568 3946 3568 Size: 378 5881 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3234 3234 Size: 6259 FspI TGC'GCA Cuts at: 4765 4765 Size: 6259 HaeII r_GCGC'y Cuts at: 1678 5406 1678 Size: 3728 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2541 2573 4900 4932 4974 Size: 32 135 32 969 32 2327 32 42 Cuts at: 4974 5006 1373 Size: 32 2626 Fragments arranged by size: 2626 2327 969 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3603 3849 669 Size: 988 281 1665 246 3079 Fragments arranged by size: 3079 1665 988 281 246 HindIII A'AGCT_T Cuts at: 748 3132 748 Size: 2384 3875 HpaI GTT'AAC Cuts at: 3849 3849 Size: 6259 KpnI G_GTAC'C Cuts at: 3584 3584 Size: 6259 MfeI C'AATT_G Cuts at: 3858 3858 Size: 6259 MluI A'CGCG_T Cuts at: 6135 6135 Size: 6259 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2835 5373 5557 1305 Size: 1530 2538 184 2007 Fragments arranged by size: 2538 2007 1530 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3208 3332 10 Size: 54 1425 343 1376 124 2937 Fragments arranged by size: 2937 1425 1376 343 124 54 MslI CAynn'nnrTG Cuts at: 518 1166 2222 3245 4217 4576 4735 518 Size: 648 1056 1023 972 359 159 2042 Fragments arranged by size: 2042 1056 1023 972 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3127 3195 3231 3352 3675 4352 5500 5745 Size: 1521 68 36 121 323 677 1148 245 Cuts at: 5745 1606 Size: 2120 Fragments arranged by size: 2120 1521 1148 677 323 245 121 68 36 NcoI C'CATG_G Cuts at: 513 1065 2004 3255 3337 513 Size: 552 939 1251 82 3435 Fragments arranged by size: 3435 1251 939 552 82 NdeI CA'TA_TG Cuts at: 387 387 Size: 6259 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6259 NotI GC'GGCC_GC Cuts at: 3706 3706 Size: 6259 NspI r_CATG'y Cuts at: 1132 1229 3142 3217 3617 5162 1132 Size: 97 1913 75 400 1545 2229 Fragments arranged by size: 2229 1913 1545 400 97 75 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6259 PciI A'CATG_T Cuts at: 5158 5158 Size: 6259 PflMI CCAn_nnn'nTGG Cuts at: 1108 2212 3400 1108 Size: 1104 1188 3967 Fragments arranged by size: 3967 1188 1104 PmeI GTTT'AAAC Cuts at: 3563 3563 Size: 6259 PmlI CAC'GTG Cuts at: 3204 3204 Size: 6259 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3350 3382 4328 4360 5841 5873 3350 Size: 32 946 32 1481 32 3736 Fragments arranged by size: 3736 1481 946 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2868 2911 2868 Size: 43 6216 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6259 PsiI TTA'TAA Cuts at: 3829 3829 Size: 6259 PspOMI G'GGCC_C Cuts at: 2103 3302 2103 Size: 1199 5060 PstI C_TGCA'G Cuts at: 830 3435 3611 830 Size: 2605 176 3478 Fragments arranged by size: 3478 2605 176 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6259 PvuII CAG'CTG Cuts at: 3195 3231 3352 3195 Size: 36 121 6102 Fragments arranged by size: 6102 121 36 SacI G_AGCT'C Cuts at: 2845 2845 Size: 6259 SalI G'TCGA_C Cuts at: 1936 3601 1936 Size: 1665 4594 SanDI GG'GwC_CC Cuts at: 2868 2868 Size: 6259 SbfI CC_TGCA'GG Cuts at: 3611 3611 Size: 6259 ScaI AGT'ACT Cuts at: 4507 6219 4507 Size: 1712 4547 SfcI C'TryA_G Cuts at: 826 1045 3106 3223 3431 3607 4742 5423 5614 Size: 219 2061 117 208 176 1135 681 191 Cuts at: 5614 826 Size: 1471 Fragments arranged by size: 2061 1471 1135 681 219 208 191 176 117 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6259 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6259 SmaI CCC'GGG Cuts at: 1872 3586 1872 Size: 1714 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2477 2838 4363 5264 5526 820 Size: 197 926 534 361 1525 901 262 1553 Fragments arranged by size: 1553 1525 926 901 534 361 262 197 SnaBI TAC'GTA Cuts at: 493 4050 493 Size: 3557 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6259 SphI G_CATG'C Cuts at: 1132 3217 3617 1132 Size: 2085 400 3774 Fragments arranged by size: 3774 2085 400 SspI AAT'ATT Cuts at: 5 52 4183 5 Size: 47 4131 2081 Fragments arranged by size: 4131 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6259 StyI C'CwwG_G Cuts at: 513 1065 2004 2235 3255 3337 3485 3698 513 Size: 552 939 231 1020 82 148 213 3074 Fragments arranged by size: 3074 1020 939 552 231 213 148 82 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2159 2433 2941 3058 3086 4286 4303 Size: 1255 579 274 508 117 28 1200 17 Cuts at: 4303 4456 4641 5086 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1255 1200 579 508 445 274 185 153 117 28 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2301 4505 6217 Size: 274 80 33 51 1443 324 2204 1712 Cuts at: 6217 96 Size: 138 Fragments arranged by size: 2204 1712 1443 324 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2154 2186 2408 2440 2995 3027 3365 3397 6219 Size: 32 222 32 555 32 338 32 2822 Cuts at: 6219 6251 2154 Size: 32 2162 Fragments arranged by size: 2822 2162 555 338 222 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3115 1316 Size: 1799 4460 XbaI T'CTAG_A Cuts at: 3595 3595 Size: 6259 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2494 2885 2494 Size: 391 5868 XhoI C'TCGA_G Cuts at: 1943 2838 1943 Size: 895 5364 XmaI C'CCGG_G Cuts at: 1870 3584 1870 Size: 1714 4545 XmnI GAAnn'nnTTC Cuts at: 4388 4388 Size: 6259 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4067 276 Size: 53 83 186 936 2533 2468 Fragments arranged by size: 2533 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PspXI PsrI RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI