Restriction map for
pFN21AB5967
(Linear) (Six-base) MAPSORT of: KIBB5967.seq Check: 9598 from: 1 to: 1554
KIBB5967.orf
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1429 1554
Size: 1429 125
AflIII A'CryG_T
Cuts at: 0 1196 1554
Size: 1196 358
AleI CACnn'nnGTG
Cuts at: 0 1240 1554
Size: 1240 314
AlwNI CAG_nnn'CTG
Cuts at: 0 1089 1226 1554
Size: 1089 137 328
Fragments arranged by size:
1089 328 137
ApaI G_GGCC'C
Cuts at: 0 102 1301 1554
Size: 102 1199 253
Fragments arranged by size:
1199 253 102
ApaLI G'TGCA_C
Cuts at: 0 409 1513 1554
Size: 409 1104 41
Fragments arranged by size:
1104 409 41
ApoI r'AATT_y
Cuts at: 0 196 343 519 956 1554
Size: 196 147 176 437 598
Fragments arranged by size:
598 437 196 176 147
AvaI C'yCGr_G
Cuts at: 0 82 94 833 1293 1554
Size: 82 12 739 460 261
Fragments arranged by size:
739 460 261 82 12
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 951 984 1554
Size: 951 33 570
Fragments arranged by size:
951 570 33
BanI G'GyrC_C
Cuts at: 0 1093 1438 1530 1554
Size: 1093 345 92 24
Fragments arranged by size:
1093 345 92 24
BanII G_rGCy'C
Cuts at: 0 102 386 840 1301 1554
Size: 102 284 454 461 253
Fragments arranged by size:
461 454 284 253 102
BclI T'GATC_A
Cuts at: 0 221 1554
Size: 221 1333
BglI GCCn_nnn'nGGC
Cuts at: 0 433 1554
Size: 433 1121
BlpI GC'TnA_GC
Cuts at: 0 380 1554
Size: 380 1174
Bme1580I G_kGCm'C
Cuts at: 0 102 214 413 1172 1301 1517 1554
Size: 102 112 199 759 129 216 37
Fragments arranged by size:
759 216 199 129 112 102 37
BmgBI CAC'GTC
Cuts at: 0 612 1554
Size: 612 942
BmrI ACTGGGnnnn_n'
Cuts at: 0 248 1034 1554
Size: 248 786 520
Fragments arranged by size:
786 520 248
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 1554
Size: 64 1490
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 457 1554
Size: 457 1097
BsaAI yAC'GTr
Cuts at: 0 408 1199 1554
Size: 408 791 355
Fragments arranged by size:
791 408 355
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 403 433 1321 1351 1360 1390 1554
Size: 403 30 888 30 9 30 164
Fragments arranged by size:
888 403 164 30 30 30 9
BseYI C'CCAG_C
Cuts at: 0 103 140 1156 1171 1347 1554
Size: 103 37 1016 15 176 207
Fragments arranged by size:
1016 207 176 103 37 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 588 715 1212 1264 1554
Size: 588 127 497 52 290
Fragments arranged by size:
588 497 290 127 52
BsiHKAI G_wGCw'C
Cuts at: 0 413 784 840 1517 1554
Size: 413 371 56 677 37
Fragments arranged by size:
677 413 371 56 37
BsmI GAATG_Cn'
Cuts at: 0 752 1554
Size: 752 802
BsmBI CGTCTCn'nnnn_
Cuts at: 0 462 1554
Size: 462 1092
Bsp1286I G_dGCh'C
Cuts at: 0 102 214 386 413 784 840 1172 1301
Size: 102 112 172 27 371 56 332 129
Cuts at: 1301 1517 1554
Size: 216 37
Fragments arranged by size:
371 332 216 172 129 112 102 56
37 27
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 71 330 1554
Size: 71 259 1224
Fragments arranged by size:
1224 259 71
BsrBI CCG'CTC
Cuts at: 0 1064 1282 1554
Size: 1064 218 272
Fragments arranged by size:
1064 272 218
BssHII G'CGCG_C
Cuts at: 0 89 1554
Size: 89 1465
BstBI TT'CG_AA
Cuts at: 0 76 1554
Size: 76 1478
BtgI C'CryG_G
Cuts at: 0 767 1250 1332 1379 1554
Size: 767 483 82 47 175
Fragments arranged by size:
767 483 175 82 47
BtsI GCAGTG_nn'
Cuts at: 0 644 720 934 1411 1554
Size: 644 76 214 477 143
Fragments arranged by size:
644 477 214 143 76
DraIII CAC_nnn'GTG
Cuts at: 0 207 555 1042 1512 1554
Size: 207 348 487 470 42
Fragments arranged by size:
487 470 348 207 42
EaeI y'GGCC_r
Cuts at: 0 2 396 1201 1325 1554
Size: 2 394 805 124 229
Fragments arranged by size:
805 394 229 124 2
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 258 1554
Size: 258 1296
EcoICRI GAG'CTC
Cuts at: 0 838 1554
Size: 838 716
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 1429 1554
Size: 64 1365 125
Fragments arranged by size:
1365 125 64
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1101 1554
Size: 1101 453
EcoO109I rG'GnC_Cy
Cuts at: 0 98 99 863 906 1086 1297 1298 1554
Size: 98 1 764 43 180 211 1 256
Fragments arranged by size:
764 256 211 180 98 43 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1229 1554
Size: 1229 325
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 536 568 1554
Size: 536 32 986
Fragments arranged by size:
986 536 32
HindIII A'AGCT_T
Cuts at: 0 1127 1554
Size: 1127 427
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 830 1554
Size: 830 724
MscI TGG'CCA
Cuts at: 0 1203 1327 1554
Size: 1203 124 227
Fragments arranged by size:
1203 227 124
MslI CAynn'nnrTG
Cuts at: 0 217 1240 1554
Size: 217 1023 314
Fragments arranged by size:
1023 314 217
MspA1I CmG'CkG
Cuts at: 0 1122 1190 1226 1347 1554
Size: 1122 68 36 121 207
Fragments arranged by size:
1122 207 121 68 36
NcoI C'CATG_G
Cuts at: 0 1250 1332 1554
Size: 1250 82 222
Fragments arranged by size:
1250 222 82
NspI r_CATG'y
Cuts at: 0 1137 1212 1554
Size: 1137 75 342
Fragments arranged by size:
1137 342 75
PflMI CCAn_nnn'nTGG
Cuts at: 0 207 1395 1554
Size: 207 1188 159
Fragments arranged by size:
1188 207 159
PmlI CAC'GTG
Cuts at: 0 1199 1554
Size: 1199 355
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1345 1377 1554
Size: 1345 32 177
Fragments arranged by size:
1345 177 32
PpuMI rG'GwC_Cy
Cuts at: 0 863 906 1554
Size: 863 43 648
Fragments arranged by size:
863 648 43
PspOMI G'GGCC_C
Cuts at: 0 98 1297 1554
Size: 98 1199 257
Fragments arranged by size:
1199 257 98
PstI C_TGCA'G
Cuts at: 0 1430 1554
Size: 1430 124
PvuII CAG'CTG
Cuts at: 0 1190 1226 1347 1554
Size: 1190 36 121 207
Fragments arranged by size:
1190 207 121 36
SacI G_AGCT'C
Cuts at: 0 840 1554
Size: 840 714
SanDI GG'GwC_CC
Cuts at: 0 863 1554
Size: 863 691
SfcI C'TryA_G
Cuts at: 0 1101 1218 1426 1554
Size: 1101 117 208 128
Fragments arranged by size:
1101 208 128 117
SmlI C'TyrA_G
Cuts at: 0 472 833 1554
Size: 472 361 721
Fragments arranged by size:
721 472 361
SphI G_CATG'C
Cuts at: 0 1212 1554
Size: 1212 342
StyI C'CwwG_G
Cuts at: 0 230 1250 1332 1480 1554
Size: 230 1020 82 148 74
Fragments arranged by size:
1020 230 148 82 74
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 154 428 936 1053 1081 1554
Size: 154 274 508 117 28 473
Fragments arranged by size:
508 473 274 154 117 28
TatI w'GTAC_w
Cuts at: 0 296 1554
Size: 296 1258
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 149 181 403 435 990 1022 1360 1392
Size: 149 32 222 32 555 32 338 32
Cuts at: 1392 1554
Size: 162
Fragments arranged by size:
555 338 222 162 149 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 1110 1554
Size: 1110 444
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 489 880 1554
Size: 489 391 674
Fragments arranged by size:
674 489 391
XhoI C'TCGA_G
Cuts at: 0 833 1554
Size: 833 721
Enzymes that do cut:
AcuI AflIII AleI AlwNI ApaI ApaLI ApoI AvaI
BaeI BanI BanII BclI BglI BlpI Bme1580I BmgBI
BmrI BpmI BpuEI BsaAI BsaXI BseYI BsgI BsiHKAI
BsmI BsmBI Bsp1286I BspMI BsrBI BssHII BstBI BtgI
BtsI DraIII EaeI EciI EcoICRI Eco57MI EcoNI EcoO109I
FalI Hin4I HindIII MmeI MscI MslI MspA1I NcoI
NspI PflMI PmlI PpiI PpuMI PspOMI PstI PvuII
SacI SanDI SfcI SmlI SphI StyI TaqII TatI
TstI Tth111I XcmI XhoI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AlfI AloI AscI AseI AsiSI AvrII BamHI
BbeI BbsI BbvCI BcgI BciVI BfrBI BglII BmtI
BplI Bpu10I BsaI BsaBI BsaHI BsaWI BseRI BsiEI
BsiWI BspEI BspHI BsrDI BsrFI BsrGI BssSI BstAPI
BstEII BstXI BstYI BstZ17I Bsu36I BtgZI ClaI CspCI
DraI DrdI EagI EarI EcoRI EcoRV FseI FspI
FspAI HaeII HincII HpaI KasI KpnI MfeI MluI
NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PfoI PmeI PshAI PsiI PspXI
PsrI PvuI RsrII SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SrfI SspI StuI SwaI XbaI XmaI XmnI ZraI