(Circular) (Six-base) MAPSORT of: pFN21AB5940.seq Check: 5238 from: 1 to: 6325 pFN21AB5940 6325 bp With 182 enzymes: SgfI * April 15, 2010 09:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2475 2475 Size: 6325 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4135 278 Size: 53 83 186 936 2599 2468 Fragments arranged by size: 2599 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2340 3668 1937 Size: 403 1328 4594 Fragments arranged by size: 4594 1328 403 Acc65I G'GTAC_C Cuts at: 3646 3646 Size: 6325 AclI AA'CG_TT Cuts at: 1563 4452 4825 1563 Size: 2889 373 3063 Fragments arranged by size: 3063 2889 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2441 3204 4388 5772 6040 1632 Size: 809 763 1184 1384 268 1917 Fragments arranged by size: 1917 1384 1184 809 763 268 AfeI AGC'GCT Cuts at: 2151 2151 Size: 6325 AflII C'TTAA_G Cuts at: 820 1017 3023 820 Size: 197 2006 4122 Fragments arranged by size: 4122 2006 197 AflIII A'CryG_T Cuts at: 1203 5224 6201 1203 Size: 4021 977 1327 Fragments arranged by size: 4021 1327 977 AgeI A'CCGG_T Cuts at: 3306 5156 3306 Size: 1850 4475 AhdI GACnn_n'nnGTC Cuts at: 5054 5054 Size: 6325 AleI CACnn'nnGTG Cuts at: 1166 2478 1166 Size: 1312 5013 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6325 AlwNI CAG_nnn'CTG Cuts at: 4068 5640 4068 Size: 1572 4753 ApaI G_GGCC'C Cuts at: 2952 2952 Size: 6325 ApaLI G'TGCA_C Cuts at: 4382 5538 4382 Size: 1156 5169 ApoI r'AATT_y Cuts at: 1493 2311 2712 2763 2849 3124 3333 3634 3860 Size: 818 401 51 86 275 209 301 226 Cuts at: 3860 4012 6121 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 818 401 301 275 226 209 152 86 51 AseI AT'TA_AT Cuts at: 160 1032 4879 160 Size: 872 3847 1606 Fragments arranged by size: 3847 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6325 AvaI C'yCGr_G Cuts at: 1870 1943 2055 2394 2952 3650 1870 Size: 73 112 339 558 698 4545 Fragments arranged by size: 4545 698 558 339 112 73 BamHI G'GATC_C Cuts at: 3655 4022 3655 Size: 367 5958 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2122 3646 5101 618 Size: 325 217 627 335 1524 1455 1842 Fragments arranged by size: 1842 1524 1455 627 335 325 217 BanII G_rGCy'C Cuts at: 1391 2952 3284 3644 1391 Size: 1561 332 360 4072 Fragments arranged by size: 4072 1561 360 332 BbeI G_GCGC'C Cuts at: 2126 2126 Size: 6325 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2505 928 Size: 971 606 4748 Fragments arranged by size: 4748 971 606 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3001 3035 4516 4550 1903 Size: 34 1064 34 1481 34 3678 Fragments arranged by size: 3678 1481 1064 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4217 5427 4217 Size: 1210 5115 BclI T'GATC_A Cuts at: 1548 1548 Size: 6325 BfrBI ATG'CAT Cuts at: 3058 3058 Size: 6325 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4936 136 Size: 107 122 71 631 605 3264 1525 Fragments arranged by size: 3264 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2209 2783 3538 6320 2209 Size: 574 755 2782 2214 Fragments arranged by size: 2782 2214 755 574 BlpI GC'TnA_GC Cuts at: 3351 3742 3351 Size: 391 5934 Bme1580I G_kGCm'C Cuts at: 1790 2952 3251 4386 5542 1790 Size: 1162 299 1135 1156 2573 Fragments arranged by size: 2573 1162 1156 1135 299 BmgBI CAC'GTC Cuts at: 1318 2997 1318 Size: 1679 4646 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2373 2542 4056 Size: 416 53 83 399 24 956 169 1514 Cuts at: 4056 5014 442 Size: 958 1753 Fragments arranged by size: 1753 1514 958 956 416 399 169 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6325 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4985 1239 Size: 3746 2579 Bpu10I CC'TnA_GC Cuts at: 2253 2253 Size: 6325 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4450 5315 5613 4450 Size: 865 298 5162 Fragments arranged by size: 5162 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2949 4988 882 Size: 617 1450 2039 2219 Fragments arranged by size: 2219 2039 1450 617 BsaAI yAC'GTr Cuts at: 493 1204 4116 493 Size: 711 2912 2702 Fragments arranged by size: 2912 2702 711 BsaBI GATnn'nnATC Cuts at: 6319 6319 Size: 6325 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2109 2123 4132 4514 Size: 53 83 186 936 576 14 2009 382 Cuts at: 4514 5149 275 Size: 635 1451 Fragments arranged by size: 2009 1451 936 635 576 382 186 83 53 14 BsaWI w'CCGG_w Cuts at: 2164 2221 3306 4758 5156 5430 5577 5988 2164 Size: 57 1085 1452 398 274 147 411 2501 Fragments arranged by size: 2501 1452 1085 411 398 274 147 57 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6100 Fragments arranged by size: 6100 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6325 BseYI C'CCAG_C Cuts at: 3000 3312 5528 6024 3000 Size: 312 2216 496 3301 Fragments arranged by size: 3301 2216 496 312 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2123 2994 1163 Size: 573 387 871 4494 Fragments arranged by size: 4494 871 573 387 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3775 4536 5564 1540 Size: 275 186 1774 761 1028 2301 Fragments arranged by size: 2301 1774 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2466 2905 3284 4386 4471 5542 2466 Size: 439 379 1102 85 1071 3249 Fragments arranged by size: 3249 1102 1071 439 379 85 BsmI GAATG_Cn' Cuts at: 3836 3929 3836 Size: 93 6232 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2466 2905 2952 3251 3284 3644 4386 Size: 399 676 439 47 299 33 360 742 Cuts at: 4386 4471 5542 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 676 439 399 360 299 85 47 33 BspEI T'CCGG_A Cuts at: 2164 2221 5988 2164 Size: 57 3767 2501 Fragments arranged by size: 3767 2501 57 BspHI T'CATG_A Cuts at: 4214 5944 4214 Size: 1730 4595 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2475 3680 844 Size: 1631 1205 3489 Fragments arranged by size: 3489 1631 1205 BsrBI CCG'CTC Cuts at: 1394 1823 4212 1394 Size: 429 2389 3507 Fragments arranged by size: 3507 2389 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3144 4820 4994 65 Size: 1183 1896 1676 174 1396 Fragments arranged by size: 1896 1676 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2125 3306 4969 5156 1700 Size: 110 315 1181 1663 187 2869 Fragments arranged by size: 2869 1663 1181 315 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6325 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6325 BssSI C'ACGA_G Cuts at: 4385 5397 4385 Size: 1012 5313 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6325 BstBI TT'CG_AA Cuts at: 5129 5129 Size: 6325 BstEII G'GTnAC_C Cuts at: 1184 3004 1184 Size: 1820 4505 BstXI CCAn_nnnn'nTGG Cuts at: 2084 3295 2084 Size: 1211 5114 BstYI r'GATC_y Cuts at: 1972 2209 2783 3538 3655 4022 4407 4424 5876 Size: 237 574 755 117 367 385 17 1452 Cuts at: 5876 5962 5974 6320 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 755 574 385 367 346 237 117 86 17 12 BstZ17I GTA'TAC Cuts at: 2341 2341 Size: 6325 BtgI C'CryG_G Cuts at: 513 1065 2176 2825 3317 513 Size: 552 1111 649 492 3521 Fragments arranged by size: 3521 1111 649 552 492 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5586 Fragments arranged by size: 5586 683 56 BtsI GCAGTG_nn' Cuts at: 3264 3841 4635 4655 3264 Size: 577 794 20 4934 Fragments arranged by size: 4934 794 577 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6325 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6290 DraI TTT'AAA Cuts at: 2596 3629 2596 Size: 1033 5292 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5332 809 Size: 507 4016 1802 Fragments arranged by size: 4016 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2070 3520 3772 Size: 54 1425 50 275 18 240 1450 252 Cuts at: 3772 4661 6290 8 Size: 889 1629 43 Fragments arranged by size: 1629 1450 1425 889 275 252 240 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3772 1537 Size: 275 1960 4090 Fragments arranged by size: 4090 1960 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2976 3277 4255 1353 Size: 1623 301 978 3423 Fragments arranged by size: 3423 1623 978 301 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4912 5286 5432 387 Size: 1064 138 3323 374 146 1280 Fragments arranged by size: 3323 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3282 3282 Size: 6325 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2441 3204 4388 4985 5772 6040 1239 Size: 393 809 763 1184 597 787 268 1524 Fragments arranged by size: 1524 1184 809 787 763 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2475 2475 Size: 6325 EcoO109I rG'GnC_Cy Cuts at: 2172 2948 2949 2172 Size: 776 1 5548 Fragments arranged by size: 5548 776 1 EcoRI G'AATT_C Cuts at: 3124 3634 4012 3124 Size: 510 378 5437 Fragments arranged by size: 5437 510 378 FspI TGC'GCA Cuts at: 4831 4831 Size: 6325 HaeII r_GCGC'y Cuts at: 1678 2037 2091 2126 2153 5472 1678 Size: 359 54 35 27 3319 2531 Fragments arranged by size: 3319 2531 359 54 35 27 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4966 4998 5040 5072 1373 Size: 32 135 32 3394 32 42 32 2626 Fragments arranged by size: 3394 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3669 3915 669 Size: 988 281 1731 246 3079 Fragments arranged by size: 3079 1731 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6325 HpaI GTT'AAC Cuts at: 3915 3915 Size: 6325 KasI G'GCGC_C Cuts at: 2122 2122 Size: 6325 KpnI G_GTAC'C Cuts at: 3650 3650 Size: 6325 MfeI C'AATT_G Cuts at: 3924 3924 Size: 6325 MluI A'CGCG_T Cuts at: 6201 6201 Size: 6325 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2553 5439 5623 1305 Size: 1248 2886 184 2007 Fragments arranged by size: 2886 2007 1248 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3522 10 Size: 54 1425 343 1690 2813 Fragments arranged by size: 2813 1690 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2478 3293 3606 4283 4642 4801 518 Size: 648 1312 815 313 677 359 159 2042 Fragments arranged by size: 2042 1312 815 677 648 359 313 159 MspA1I CmG'CkG Cuts at: 1606 2068 2178 2410 2448 2827 3040 3550 3741 Size: 462 110 232 38 379 213 510 191 Cuts at: 3741 4418 5566 5811 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 510 462 379 245 232 213 191 110 38 NaeI GCC'GGC Cuts at: 2127 2127 Size: 6325 NarI GG'CG_CC Cuts at: 2123 2123 Size: 6325 NcoI C'CATG_G Cuts at: 513 1065 3317 513 Size: 552 2252 3521 Fragments arranged by size: 3521 2252 552 NdeI CA'TA_TG Cuts at: 387 2625 387 Size: 2238 4087 NgoMIV G'CCGG_C Cuts at: 2125 2125 Size: 6325 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6325 NotI GC'GGCC_GC Cuts at: 3772 3772 Size: 6325 NsiI A_TGCA'T Cuts at: 3060 3060 Size: 6325 NspI r_CATG'y Cuts at: 1132 1229 2732 3037 3683 5228 1132 Size: 97 1503 305 646 1545 2229 Fragments arranged by size: 2229 1545 1503 646 305 97 PasI CC'CwG_GG Cuts at: 1348 2095 1348 Size: 747 5578 PciI A'CATG_T Cuts at: 5224 5224 Size: 6325 PflMI CCAn_nnn'nTGG Cuts at: 1108 2119 2509 3511 1108 Size: 1011 390 1002 3922 Fragments arranged by size: 3922 1011 1002 390 PmeI GTTT'AAAC Cuts at: 3629 3629 Size: 6325 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4394 4426 5907 5939 4394 Size: 32 1481 32 4780 Fragments arranged by size: 4780 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2172 2172 Size: 6325 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6325 PsiI TTA'TAA Cuts at: 3895 3895 Size: 6325 PspOMI G'GGCC_C Cuts at: 2948 2948 Size: 6325 PstI C_TGCA'G Cuts at: 830 2191 3086 3677 830 Size: 1361 895 591 3478 Fragments arranged by size: 3478 1361 895 591 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6325 PvuII CAG'CTG Cuts at: 2410 2448 3040 3550 2410 Size: 38 592 510 5185 Fragments arranged by size: 5185 592 510 38 SacI G_AGCT'C Cuts at: 3284 3284 Size: 6325 SacII CC_GC'GG Cuts at: 2179 2828 2179 Size: 649 5676 SalI G'TCGA_C Cuts at: 1936 3667 1936 Size: 1731 4594 SanDI GG'GwC_CC Cuts at: 2172 2172 Size: 6325 SapI GCTCTTCn'nnn_ Cuts at: 2976 2976 Size: 6325 SbfI CC_TGCA'GG Cuts at: 3677 3677 Size: 6325 ScaI AGT'ACT Cuts at: 4573 6285 4573 Size: 1712 4613 SfcI C'TryA_G Cuts at: 826 1045 2187 3082 3673 4808 5489 5680 826 Size: 219 1142 895 591 1135 681 191 1471 Fragments arranged by size: 1471 1142 1135 895 681 591 219 191 SfoI GGC'GCC Cuts at: 2124 2124 Size: 6325 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6325 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6325 SmaI CCC'GGG Cuts at: 1872 2057 3652 1872 Size: 185 1595 4545 Fragments arranged by size: 4545 1595 185 SmlI C'TyrA_G Cuts at: 820 1017 1943 2394 3023 4429 5330 5592 820 Size: 197 926 451 629 1406 901 262 1553 Fragments arranged by size: 1553 1406 926 901 629 451 262 197 SnaBI TAC'GTA Cuts at: 493 4116 493 Size: 3623 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6325 SphI G_CATG'C Cuts at: 1132 3037 3683 1132 Size: 1905 646 3774 Fragments arranged by size: 3774 1905 646 SspI AAT'ATT Cuts at: 5 52 4249 5 Size: 47 4197 2081 Fragments arranged by size: 4197 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6325 StyI C'CwwG_G Cuts at: 513 1065 3317 3764 513 Size: 552 2252 447 3074 Fragments arranged by size: 3074 2252 552 447 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2992 4352 4369 4522 4707 5152 325 Size: 1255 1412 1360 17 153 185 445 1498 Fragments arranged by size: 1498 1412 1360 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4571 6283 96 Size: 274 80 33 51 1443 2594 1712 138 Fragments arranged by size: 2594 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6285 6317 6285 Size: 32 6293 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6325 XbaI T'CTAG_A Cuts at: 3338 3661 3338 Size: 323 6002 XhoI C'TCGA_G Cuts at: 1943 2394 1943 Size: 451 5874 XmaI C'CCGG_G Cuts at: 1870 2055 3650 1870 Size: 185 1595 4545 Fragments arranged by size: 4545 1595 185 XmnI GAAnn'nnTTC Cuts at: 4454 4454 Size: 6325 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4133 276 Size: 53 83 186 936 2599 2468 Fragments arranged by size: 2599 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbvCI BplI BsiWI BsmBI Bsu36I DraIII EcoRV FalI FseI FspAI NruI PacI PfoI PmlI PspXI PsrI RsrII SexAI SfiI SrfI SwaI XcmI