(Circular) (Six-base) MAPSORT of: pFN21AB5935.seq Check: 451 from: 1 to: 6229 pFN21AB5935 6229 bp With 182 enzymes: SgfI * April 15, 2010 09:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4039 278 Size: 53 83 186 936 2503 2468 Fragments arranged by size: 2503 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3572 1937 Size: 1635 4594 Acc65I G'GTAC_C Cuts at: 3550 3550 Size: 6229 AclI AA'CG_TT Cuts at: 1563 4356 4729 1563 Size: 2793 373 3063 Fragments arranged by size: 3063 2793 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2517 2763 4292 5676 5944 1632 Size: 885 246 1529 1384 268 1917 Fragments arranged by size: 1917 1529 1384 885 268 246 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6032 AflIII A'CryG_T Cuts at: 1203 2301 5128 6105 1203 Size: 1098 2827 977 1327 Fragments arranged by size: 2827 1327 1098 977 AgeI A'CCGG_T Cuts at: 5060 5060 Size: 6229 AhdI GACnn_n'nnGTC Cuts at: 4958 4958 Size: 6229 AleI CACnn'nnGTG Cuts at: 1166 2636 1166 Size: 1470 4759 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2654 1184 Size: 1470 4759 AlwNI CAG_nnn'CTG Cuts at: 3972 5544 3972 Size: 1572 4657 ApaLI G'TGCA_C Cuts at: 4286 5442 4286 Size: 1156 5073 ApoI r'AATT_y Cuts at: 1493 2753 3454 3538 3764 3916 6025 1493 Size: 1260 701 84 226 152 2109 1697 Fragments arranged by size: 2109 1697 1260 701 226 152 84 AseI AT'TA_AT Cuts at: 160 1032 2076 4783 160 Size: 872 1044 2707 1606 Fragments arranged by size: 2707 1606 1044 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6229 AvaI C'yCGr_G Cuts at: 1870 1943 3554 1870 Size: 73 1611 4545 Fragments arranged by size: 4545 1611 73 BamHI G'GATC_C Cuts at: 2719 3559 3926 2719 Size: 840 367 5022 Fragments arranged by size: 5022 840 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3550 5005 618 Size: 325 217 627 1763 1455 1842 Fragments arranged by size: 1842 1763 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3548 1391 Size: 2157 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5258 BbvCI CC'TCA_GC Cuts at: 2571 2571 Size: 6229 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3422 3456 4420 4454 1903 Size: 34 1485 34 964 34 3678 Fragments arranged by size: 3678 1485 964 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4121 5331 4121 Size: 1210 5019 BclI T'GATC_A Cuts at: 1548 1548 Size: 6229 BfrBI ATG'CAT Cuts at: 3172 3172 Size: 6229 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4840 136 Size: 107 122 71 631 605 3168 1525 Fragments arranged by size: 3168 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6224 6224 Size: 6229 BlpI GC'TnA_GC Cuts at: 3646 3646 Size: 6229 Bme1580I G_kGCm'C Cuts at: 1790 2281 4290 5446 1790 Size: 491 2009 1156 2573 Fragments arranged by size: 2573 2009 1156 491 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6229 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3960 4918 442 Size: 416 53 83 399 24 2543 958 1753 Fragments arranged by size: 2543 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6229 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2403 2828 4889 1239 Size: 1164 425 2061 2579 Fragments arranged by size: 2579 2061 1164 425 Bpu10I CC'TnA_GC Cuts at: 2571 2571 Size: 6229 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4354 5219 5517 4354 Size: 865 298 5066 Fragments arranged by size: 5066 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4892 882 Size: 617 3393 2219 Fragments arranged by size: 3393 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4020 493 Size: 711 2816 2702 Fragments arranged by size: 2816 2702 711 BsaBI GATnn'nnATC Cuts at: 2052 3428 6223 2052 Size: 1376 2795 2058 Fragments arranged by size: 2795 2058 1376 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3238 3280 4036 4418 Size: 53 83 186 936 1705 42 756 382 Cuts at: 4418 5053 275 Size: 635 1451 Fragments arranged by size: 1705 1451 936 756 635 382 186 83 53 42 BsaWI w'CCGG_w Cuts at: 4662 5060 5334 5481 5892 4662 Size: 398 274 147 411 4999 Fragments arranged by size: 4999 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2959 2989 1178 Size: 30 165 30 1556 30 4418 Fragments arranged by size: 4418 1556 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6229 BseYI C'CCAG_C Cuts at: 5432 5928 5432 Size: 496 5733 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2304 1163 Size: 573 568 5088 Fragments arranged by size: 5088 573 568 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3679 4440 5468 1540 Size: 275 186 1678 761 1028 2301 Fragments arranged by size: 2301 1678 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2524 3217 4290 4375 5446 2524 Size: 693 1073 85 1071 3307 Fragments arranged by size: 3307 1073 1071 693 85 BsmI GAATG_Cn' Cuts at: 3740 3833 3740 Size: 93 6136 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2281 2524 3217 3548 4290 4375 5446 Size: 399 491 243 693 331 742 85 1071 Cuts at: 5446 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 693 491 399 331 243 85 BspEI T'CCGG_A Cuts at: 5892 5892 Size: 6229 BspHI T'CATG_A Cuts at: 4118 5848 4118 Size: 1730 4499 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3584 844 Size: 2740 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2620 4116 1394 Size: 429 797 1496 3507 Fragments arranged by size: 3507 1496 797 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4724 4898 65 Size: 1183 3476 174 1396 Fragments arranged by size: 3476 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4873 5060 1700 Size: 110 3063 187 2869 Fragments arranged by size: 3063 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3248 96 Size: 3152 3077 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6229 BssSI C'ACGA_G Cuts at: 3474 4289 5301 3474 Size: 815 1012 4402 Fragments arranged by size: 4402 1012 815 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6229 BstBI TT'CG_AA Cuts at: 5033 5033 Size: 6229 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6229 BstYI r'GATC_y Cuts at: 1972 2719 3163 3559 3926 4311 4328 5780 5866 Size: 747 444 396 367 385 17 1452 86 Cuts at: 5866 5878 6224 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 747 444 396 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2956 2956 Size: 6229 BtgI C'CryG_G Cuts at: 513 1065 2004 3525 513 Size: 552 939 1521 3217 Fragments arranged by size: 3217 1521 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5490 Fragments arranged by size: 5490 683 56 BtsI GCAGTG_nn' Cuts at: 3343 3467 3745 4539 4559 3343 Size: 124 278 794 20 5013 Fragments arranged by size: 5013 794 278 124 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6229 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6194 DraI TTT'AAA Cuts at: 3533 3533 Size: 6229 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5236 809 Size: 507 3920 1802 Fragments arranged by size: 3920 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3676 4565 6194 Size: 54 1425 50 275 18 1846 889 1629 Cuts at: 6194 8 Size: 43 Fragments arranged by size: 1846 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3676 1537 Size: 275 1864 4090 Fragments arranged by size: 4090 1864 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2340 2610 2815 2884 4159 1353 Size: 987 270 205 69 1275 3423 Fragments arranged by size: 3423 1275 987 270 205 69 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4816 5190 5336 387 Size: 1064 138 3227 374 146 1280 Fragments arranged by size: 3227 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2403 2517 2763 2828 4292 4889 5676 Size: 393 771 114 246 65 1464 597 787 Cuts at: 5676 5944 1239 Size: 268 1524 Fragments arranged by size: 1524 1464 787 771 597 393 268 246 114 65 EcoNI CCTnn'n_nnAGG Cuts at: 2535 2535 Size: 6229 EcoRI G'AATT_C Cuts at: 3538 3916 3538 Size: 378 5851 EcoRV GAT'ATC Cuts at: 2453 2462 3274 2453 Size: 9 812 5408 Fragments arranged by size: 5408 812 9 FspI TGC'GCA Cuts at: 4735 4735 Size: 6229 HaeII r_GCGC'y Cuts at: 1678 5376 1678 Size: 3698 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2244 2276 2468 2500 2885 Size: 32 135 32 672 32 192 32 385 Cuts at: 2885 2917 2959 2991 3121 3153 3473 3505 4870 Size: 32 42 32 130 32 320 32 1365 Cuts at: 4870 4902 4944 4976 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1365 672 385 320 192 135 130 42 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2596 2908 3322 3573 3819 669 Size: 988 281 658 312 414 251 246 3079 Fragments arranged by size: 3079 988 658 414 312 281 251 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6229 HpaI GTT'AAC Cuts at: 2908 3819 2908 Size: 911 5318 KpnI G_GTAC'C Cuts at: 3554 3554 Size: 6229 MfeI C'AATT_G Cuts at: 3828 3828 Size: 6229 MluI A'CGCG_T Cuts at: 6105 6105 Size: 6229 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5343 5527 1305 Size: 4038 184 2007 Fragments arranged by size: 4038 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4407 Fragments arranged by size: 4407 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2078 2589 2636 3177 4187 4546 4705 Size: 648 912 511 47 541 1010 359 159 Cuts at: 4705 518 Size: 2042 Fragments arranged by size: 2042 1010 912 648 541 511 359 159 47 MspA1I CmG'CkG Cuts at: 1606 3645 4322 5470 5715 1606 Size: 2039 677 1148 245 2120 Fragments arranged by size: 2120 2039 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 3525 513 Size: 552 939 1521 3217 Fragments arranged by size: 3217 1521 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6229 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6229 NotI GC'GGCC_GC Cuts at: 3676 3676 Size: 6229 NsiI A_TGCA'T Cuts at: 3174 3174 Size: 6229 NspI r_CATG'y Cuts at: 1132 1229 2305 2645 3587 5132 1132 Size: 97 1076 340 942 1545 2229 Fragments arranged by size: 2229 1545 1076 942 340 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6229 PciI A'CATG_T Cuts at: 2301 5128 2301 Size: 2827 3402 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6229 PfoI T'CCnGG_A Cuts at: 2419 2419 Size: 6229 PmeI GTTT'AAAC Cuts at: 3533 3533 Size: 6229 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4298 4330 5811 5843 4298 Size: 32 1481 32 4684 Fragments arranged by size: 4684 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6229 PsiI TTA'TAA Cuts at: 3799 3799 Size: 6229 PstI C_TGCA'G Cuts at: 830 3581 830 Size: 2751 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6229 SalI G'TCGA_C Cuts at: 1936 3571 1936 Size: 1635 4594 SapI GCTCTTCn'nnn_ Cuts at: 2340 2610 2340 Size: 270 5959 SbfI CC_TGCA'GG Cuts at: 3581 3581 Size: 6229 ScaI AGT'ACT Cuts at: 3514 4477 6189 3514 Size: 963 1712 3554 Fragments arranged by size: 3554 1712 963 SfcI C'TryA_G Cuts at: 826 1045 2601 3577 4712 5393 5584 826 Size: 219 1556 976 1135 681 191 1471 Fragments arranged by size: 1556 1471 1135 976 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6229 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6229 SmaI CCC'GGG Cuts at: 1872 3556 1872 Size: 1684 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4333 5234 5496 820 Size: 197 926 2390 901 262 1553 Fragments arranged by size: 2390 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4020 493 Size: 3527 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6229 SphI G_CATG'C Cuts at: 1132 3587 1132 Size: 2455 3774 SspI AAT'ATT Cuts at: 5 52 3230 4153 5 Size: 47 3178 923 2081 Fragments arranged by size: 3178 2081 923 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6229 StyI C'CwwG_G Cuts at: 513 1065 2004 3525 3668 513 Size: 552 939 1521 143 3074 Fragments arranged by size: 3074 1521 939 552 143 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4256 4273 4426 4611 5056 325 Size: 1255 2676 17 153 185 445 1498 Fragments arranged by size: 2676 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2581 3248 3512 Size: 274 80 33 51 1443 604 667 264 Cuts at: 3512 4475 6187 96 Size: 963 1712 138 Fragments arranged by size: 1712 1443 963 667 604 274 264 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6189 6221 6189 Size: 32 6197 Tth111I GACn'n_nGTC Cuts at: 1316 2039 1316 Size: 723 5506 XbaI T'CTAG_A Cuts at: 3160 3565 3160 Size: 405 5824 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6229 XmaI C'CCGG_G Cuts at: 1870 3554 1870 Size: 1684 4545 XmnI GAAnn'nnTTC Cuts at: 4358 4358 Size: 6229 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4037 276 Size: 53 83 186 936 2503 2468 Fragments arranged by size: 2503 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI