(Circular) (Six-base) MAPSORT of: pFN21AB5926.seq Check: 1989 from: 1 to: 6313 pFN21AB5926 6313 bp With 182 enzymes: SgfI * April 15, 2010 09:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3376 3376 Size: 6313 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4123 278 Size: 53 83 186 936 2587 2468 Fragments arranged by size: 2587 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3656 1937 Size: 1719 4594 Acc65I G'GTAC_C Cuts at: 3634 3634 Size: 6313 AclI AA'CG_TT Cuts at: 1563 4440 4813 1563 Size: 2877 373 3063 Fragments arranged by size: 3063 2877 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2583 2909 3605 4376 5760 6028 1632 Size: 951 326 696 771 1384 268 1917 Fragments arranged by size: 1917 1384 951 771 696 326 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6116 AflIII A'CryG_T Cuts at: 1203 5212 6189 1203 Size: 4009 977 1327 Fragments arranged by size: 4009 1327 977 AgeI A'CCGG_T Cuts at: 5144 5144 Size: 6313 AhdI GACnn_n'nnGTC Cuts at: 5042 5042 Size: 6313 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6313 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3062 1184 Size: 1878 4435 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2285 2317 2567 2599 2285 Size: 32 250 32 5999 Fragments arranged by size: 5999 250 32 32 AlwNI CAG_nnn'CTG Cuts at: 2101 2561 2884 2887 3245 3532 4056 5628 2101 Size: 460 323 3 358 287 524 1572 2786 Fragments arranged by size: 2786 1572 524 460 358 323 287 3 ApaI G_GGCC'C Cuts at: 3611 3611 Size: 6313 ApaLI G'TGCA_C Cuts at: 4370 5526 4370 Size: 1156 5157 ApoI r'AATT_y Cuts at: 1493 3622 3848 4000 6109 1493 Size: 2129 226 152 2109 1697 Fragments arranged by size: 2129 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4867 160 Size: 872 3835 1606 Fragments arranged by size: 3835 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6313 AvaI C'yCGr_G Cuts at: 1870 1943 2195 3638 1870 Size: 73 252 1443 4545 Fragments arranged by size: 4545 1443 252 73 BamHI G'GATC_C Cuts at: 3117 3643 4010 3117 Size: 526 367 5420 Fragments arranged by size: 5420 526 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2053 3634 5089 618 Size: 325 217 627 266 1581 1455 1842 Fragments arranged by size: 1842 1581 1455 627 325 266 217 BanII G_rGCy'C Cuts at: 1391 2028 2175 2346 3611 3632 1391 Size: 637 147 171 1265 21 4072 Fragments arranged by size: 4072 1265 637 171 147 21 BbeI G_GCGC'C Cuts at: 2057 2057 Size: 6313 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2897 928 Size: 971 998 4344 Fragments arranged by size: 4344 998 971 BbvCI CC'TCA_GC Cuts at: 2037 3006 3074 3136 2037 Size: 969 68 62 5214 Fragments arranged by size: 5214 969 68 62 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4504 4538 1903 Size: 34 2567 34 3678 Fragments arranged by size: 3678 2567 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4205 5415 4205 Size: 1210 5103 BclI T'GATC_A Cuts at: 1548 1548 Size: 6313 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2526 4924 136 Size: 107 122 71 631 605 854 2398 1525 Fragments arranged by size: 2398 1525 854 631 605 122 107 71 BglII A'GATC_T Cuts at: 3324 6308 3324 Size: 2984 3329 BlpI GC'TnA_GC Cuts at: 3730 3730 Size: 6313 Bme1580I G_kGCm'C Cuts at: 1790 2410 2992 3611 4374 5530 1790 Size: 620 582 619 763 1156 2573 Fragments arranged by size: 2573 1156 763 620 619 582 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6313 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2191 2927 4044 Size: 416 53 83 399 24 774 736 1117 Cuts at: 4044 5002 442 Size: 958 1753 Fragments arranged by size: 1753 1117 958 774 736 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6313 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2090 2267 2538 3209 3311 4973 1239 Size: 851 177 271 671 102 1662 2579 Fragments arranged by size: 2579 1662 851 671 271 177 102 Bpu10I CC'TnA_GC Cuts at: 2037 3006 3074 3136 2037 Size: 969 68 62 5214 Fragments arranged by size: 5214 969 68 62 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4438 5303 5601 4438 Size: 865 298 5150 Fragments arranged by size: 5150 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2695 3029 3515 4976 882 Size: 617 1196 334 486 1461 2219 Fragments arranged by size: 2219 1461 1196 617 486 334 BsaAI yAC'GTr Cuts at: 493 1204 4104 493 Size: 711 2900 2702 Fragments arranged by size: 2900 2702 711 BsaBI GATnn'nnATC Cuts at: 6307 6307 Size: 6313 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2054 4120 4502 5137 Size: 53 83 186 936 521 2066 382 635 Cuts at: 5137 275 Size: 1451 Fragments arranged by size: 2066 1451 936 635 521 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2014 4746 5144 5418 5565 5976 2014 Size: 2732 398 274 147 411 2351 Fragments arranged by size: 2732 2351 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2567 2597 2891 2921 1178 Size: 30 165 30 1164 30 294 30 4570 Fragments arranged by size: 4570 1164 294 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2228 2336 2585 2915 1186 Size: 1042 108 249 330 4584 Fragments arranged by size: 4584 1042 330 249 108 BseYI C'CCAG_C Cuts at: 2152 2166 2367 2515 2586 2966 2991 3316 3532 Size: 14 201 148 71 380 25 325 216 Cuts at: 3532 5516 6012 2152 Size: 1984 496 2453 Fragments arranged by size: 2453 1984 496 380 325 216 201 148 71 25 14 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2246 3022 1163 Size: 573 510 776 4454 Fragments arranged by size: 4454 776 573 510 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2223 2242 3763 4524 5552 1540 Size: 275 186 222 19 1521 761 1028 2301 Fragments arranged by size: 2301 1521 1028 761 275 222 186 19 BsiHKAI G_wGCw'C Cuts at: 2145 3225 4374 4459 5530 2145 Size: 1080 1149 85 1071 2928 Fragments arranged by size: 2928 1149 1080 1071 85 BsmI GAATG_Cn' Cuts at: 3824 3917 3824 Size: 93 6220 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2028 2145 2175 2346 2410 2992 3225 Size: 399 238 117 30 171 64 582 233 Cuts at: 3225 3611 3632 4374 4459 5530 1391 Size: 386 21 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 582 399 386 238 233 171 117 85 64 30 21 BspEI T'CCGG_A Cuts at: 5976 5976 Size: 6313 BspHI T'CATG_A Cuts at: 2689 4202 5932 2689 Size: 1513 1730 3070 Fragments arranged by size: 3070 1730 1513 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3376 3668 844 Size: 2532 292 3489 Fragments arranged by size: 3489 2532 292 BsrBI CCG'CTC Cuts at: 1394 1823 3382 4200 1394 Size: 429 1559 818 3507 Fragments arranged by size: 3507 1559 818 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3085 4808 4982 65 Size: 1183 1837 1723 174 1396 Fragments arranged by size: 1837 1723 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2050 2218 4957 5144 1700 Size: 110 240 168 2739 187 2869 Fragments arranged by size: 2869 2739 240 187 168 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6313 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6313 BssSI C'ACGA_G Cuts at: 4373 5385 4373 Size: 1012 5301 BstAPI GCAn_nnn'nTGC Cuts at: 794 2101 2884 794 Size: 1307 783 4223 Fragments arranged by size: 4223 1307 783 BstBI TT'CG_AA Cuts at: 2801 5117 2801 Size: 2316 3997 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6313 BstXI CCAn_nnnn'nTGG Cuts at: 2594 2974 3463 2594 Size: 380 489 5444 Fragments arranged by size: 5444 489 380 BstYI r'GATC_y Cuts at: 1972 3117 3324 3643 4010 4395 4412 5864 5950 Size: 1145 207 319 367 385 17 1452 86 Cuts at: 5950 5962 6308 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1145 385 367 346 319 207 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2115 2637 3572 2115 Size: 522 935 4856 Fragments arranged by size: 4856 935 522 BtgI C'CryG_G Cuts at: 513 1065 2004 2161 2451 3462 513 Size: 552 939 157 290 1011 3364 Fragments arranged by size: 3364 1011 939 552 290 157 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2310 487 Size: 683 56 1084 4490 Fragments arranged by size: 4490 1084 683 56 BtsI GCAGTG_nn' Cuts at: 3829 4623 4643 3829 Size: 794 20 5499 Fragments arranged by size: 5499 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6313 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6278 DraI TTT'AAA Cuts at: 3617 3617 Size: 6313 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2768 5320 809 Size: 507 1452 2552 1802 Fragments arranged by size: 2552 1802 1452 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2239 2313 2321 Size: 54 1425 50 275 18 409 74 8 Cuts at: 2321 3760 4649 6278 8 Size: 1439 889 1629 43 Fragments arranged by size: 1629 1439 1425 889 409 275 74 54 50 43 18 8 EagI C'GGCC_G Cuts at: 1537 1812 2239 3760 1537 Size: 275 427 1521 4090 Fragments arranged by size: 4090 1521 427 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2562 2616 2639 2670 4243 1353 Size: 1209 54 23 31 1573 3423 Fragments arranged by size: 3423 1573 1209 54 31 23 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4900 5274 5420 387 Size: 1064 138 3311 374 146 1280 Fragments arranged by size: 3311 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2090 2267 2538 2583 2909 3209 3311 Size: 393 458 177 271 45 326 300 102 Cuts at: 3311 3605 4376 4973 5760 6028 1239 Size: 294 771 597 787 268 1524 Fragments arranged by size: 1524 787 771 597 458 393 326 300 294 271 268 177 102 45 EcoNI CCTnn'n_nnAGG Cuts at: 2093 3571 2093 Size: 1478 4835 EcoO109I rG'GnC_Cy Cuts at: 2074 2111 2200 2231 2374 2445 2650 3229 3242 Size: 37 89 31 143 71 205 579 13 Cuts at: 3242 3608 2074 Size: 366 4779 Fragments arranged by size: 4779 579 366 205 143 89 71 37 31 13 EcoRI G'AATT_C Cuts at: 3622 4000 3622 Size: 378 5935 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3610 3610 Size: 6313 FspI TGC'GCA Cuts at: 4819 4819 Size: 6313 HaeII r_GCGC'y Cuts at: 1678 2057 2102 2153 3207 5460 1678 Size: 379 45 51 1054 2253 2531 Fragments arranged by size: 2531 2253 1054 379 51 45 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3105 3137 3487 3519 4954 Size: 32 135 32 1533 32 350 32 1435 Cuts at: 4954 4986 5028 5060 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1533 1435 350 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3657 3903 669 Size: 988 281 1719 246 3079 Fragments arranged by size: 3079 1719 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6313 HpaI GTT'AAC Cuts at: 3903 3903 Size: 6313 KasI G'GCGC_C Cuts at: 2053 2053 Size: 6313 KpnI G_GTAC'C Cuts at: 3638 3638 Size: 6313 MfeI C'AATT_G Cuts at: 3912 3912 Size: 6313 MluI A'CGCG_T Cuts at: 6189 6189 Size: 6313 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5427 5611 1305 Size: 4122 184 2007 Fragments arranged by size: 4122 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4491 Fragments arranged by size: 4491 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3461 4271 4630 4789 518 Size: 648 2295 810 359 159 2042 Fragments arranged by size: 2295 2042 810 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2068 2163 2884 2995 3729 4406 5554 5799 Size: 462 95 721 111 734 677 1148 245 Cuts at: 5799 1606 Size: 2120 Fragments arranged by size: 2120 1148 734 721 677 462 245 111 95 NaeI GCC'GGC Cuts at: 2052 2052 Size: 6313 NarI GG'CG_CC Cuts at: 2054 2054 Size: 6313 NcoI C'CATG_G Cuts at: 513 1065 2004 2451 3462 513 Size: 552 939 447 1011 3364 Fragments arranged by size: 3364 1011 939 552 447 NdeI CA'TA_TG Cuts at: 387 387 Size: 6313 NgoMIV G'CCGG_C Cuts at: 2050 2050 Size: 6313 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6313 NotI GC'GGCC_GC Cuts at: 3760 3760 Size: 6313 NspI r_CATG'y Cuts at: 1132 1229 3671 5216 1132 Size: 97 2442 1545 2229 Fragments arranged by size: 2442 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2030 2058 2410 1348 Size: 682 28 352 5251 Fragments arranged by size: 5251 682 352 28 PciI A'CATG_T Cuts at: 5212 5212 Size: 6313 PflMI CCAn_nnn'nTGG Cuts at: 1108 3532 1108 Size: 2424 3889 PfoI T'CCnGG_A Cuts at: 2856 2856 Size: 6313 PmeI GTTT'AAAC Cuts at: 3617 3617 Size: 6313 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2567 2599 4382 4414 5895 5927 2567 Size: 32 1783 32 1481 32 2953 Fragments arranged by size: 2953 1783 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2374 2445 2650 3242 2374 Size: 71 205 592 5445 Fragments arranged by size: 5445 592 205 71 PshAI GACnn'nnGTC Cuts at: 1613 2184 2241 3021 1613 Size: 571 57 780 4905 Fragments arranged by size: 4905 780 571 57 PsiI TTA'TAA Cuts at: 3883 3883 Size: 6313 PspOMI G'GGCC_C Cuts at: 3607 3607 Size: 6313 PspXI vC'TCGA_Gb Cuts at: 2195 2195 Size: 6313 PstI C_TGCA'G Cuts at: 830 2097 2106 2361 2880 3000 3203 3665 830 Size: 1267 9 255 519 120 203 462 3478 Fragments arranged by size: 3478 1267 519 462 255 203 120 9 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6313 PvuII CAG'CTG Cuts at: 2068 2884 2995 2068 Size: 816 111 5386 Fragments arranged by size: 5386 816 111 SacII CC_GC'GG Cuts at: 2164 2164 Size: 6313 SalI G'TCGA_C Cuts at: 1936 3655 1936 Size: 1719 4594 SanDI GG'GwC_CC Cuts at: 2650 2650 Size: 6313 SbfI CC_TGCA'GG Cuts at: 3665 3665 Size: 6313 ScaI AGT'ACT Cuts at: 4561 6273 4561 Size: 1712 4601 SfcI C'TryA_G Cuts at: 826 1045 2093 2102 2357 2876 2942 2996 3199 Size: 219 1048 9 255 519 66 54 203 Cuts at: 3199 3661 4796 5477 5668 826 Size: 462 1135 681 191 1471 Fragments arranged by size: 1471 1135 1048 681 519 462 255 219 203 191 66 54 9 SfoI GGC'GCC Cuts at: 2055 2055 Size: 6313 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6313 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6313 SmaI CCC'GGG Cuts at: 1872 3640 1872 Size: 1768 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2195 4417 5318 5580 820 Size: 197 926 252 2222 901 262 1553 Fragments arranged by size: 2222 1553 926 901 262 252 197 SnaBI TAC'GTA Cuts at: 493 4104 493 Size: 3611 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6313 SphI G_CATG'C Cuts at: 1132 3671 1132 Size: 2539 3774 SspI AAT'ATT Cuts at: 5 52 4237 5 Size: 47 4185 2081 Fragments arranged by size: 4185 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6313 StyI C'CwwG_G Cuts at: 513 1065 2004 2451 2895 3462 3752 513 Size: 552 939 447 444 567 290 3074 Fragments arranged by size: 3074 939 567 552 447 444 290 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3011 4340 4357 4510 4695 5140 325 Size: 1255 1431 1329 17 153 185 445 1498 Fragments arranged by size: 1498 1431 1329 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4559 6271 96 Size: 274 80 33 51 1443 2582 1712 138 Fragments arranged by size: 2582 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2969 3001 6273 6305 2969 Size: 32 3272 32 2977 Fragments arranged by size: 3272 2977 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6313 XbaI T'CTAG_A Cuts at: 3649 3649 Size: 6313 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3460 3460 Size: 6313 XhoI C'TCGA_G Cuts at: 1943 2195 1943 Size: 252 6061 XmaI C'CCGG_G Cuts at: 1870 3638 1870 Size: 1768 4545 XmnI GAAnn'nnTTC Cuts at: 4442 4442 Size: 6313 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4121 276 Size: 53 83 186 936 2587 2468 Fragments arranged by size: 2587 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FseI FspAI NruI NsiI PacI PmlI PsrI RsrII SacI SapI SexAI SfiI SrfI SwaI