(Circular) (Six-base) MAPSORT of: pFN21AB5925.seq Check: 7466 from: 1 to: 6217 pFN21AB5925 6217 bp With 182 enzymes: SgfI * April 15, 2010 09:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2866 2866 Size: 6217 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4027 278 Size: 53 83 186 936 2491 2468 Fragments arranged by size: 2491 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3560 1937 Size: 1623 4594 Acc65I G'GTAC_C Cuts at: 3538 3538 Size: 6217 AclI AA'CG_TT Cuts at: 1563 4344 4717 1563 Size: 2781 373 3063 Fragments arranged by size: 3063 2781 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2075 2635 4280 5664 5932 1632 Size: 443 560 1645 1384 268 1917 Fragments arranged by size: 1917 1645 1384 560 443 268 AfeI AGC'GCT Cuts at: 2954 2954 Size: 6217 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6020 AflIII A'CryG_T Cuts at: 1203 5116 6093 1203 Size: 3913 977 1327 Fragments arranged by size: 3913 1327 977 AgeI A'CCGG_T Cuts at: 5048 5048 Size: 6217 AhdI GACnn_n'nnGTC Cuts at: 4946 4946 Size: 6217 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6217 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6217 AlwNI CAG_nnn'CTG Cuts at: 2725 2974 3960 5532 2725 Size: 249 986 1572 3410 Fragments arranged by size: 3410 1572 986 249 ApaI G_GGCC'C Cuts at: 2325 2688 2812 3489 2325 Size: 363 124 677 5053 Fragments arranged by size: 5053 677 363 124 ApaLI G'TGCA_C Cuts at: 4274 5430 4274 Size: 1156 5061 ApoI r'AATT_y Cuts at: 1493 3526 3752 3904 6013 1493 Size: 2033 226 152 2109 1697 Fragments arranged by size: 2109 2033 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4771 160 Size: 872 3739 1606 Fragments arranged by size: 3739 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6217 AvaI C'yCGr_G Cuts at: 1870 1943 2325 2805 3246 3542 1870 Size: 73 382 480 441 296 4545 Fragments arranged by size: 4545 480 441 382 296 73 BamHI G'GATC_C Cuts at: 3547 3914 3547 Size: 367 5850 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3277 3538 4993 618 Size: 325 217 627 1490 261 1455 1842 Fragments arranged by size: 1842 1490 1455 627 325 261 217 BanII G_rGCy'C Cuts at: 1391 2325 2688 2812 2997 3159 3405 3489 3536 Size: 934 363 124 185 162 246 84 47 Cuts at: 3536 1391 Size: 4072 Fragments arranged by size: 4072 934 363 246 185 162 124 84 47 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5246 BbvCI CC'TCA_GC Cuts at: 2340 3076 2340 Size: 736 5481 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2953 2987 4408 4442 1903 Size: 34 1016 34 1421 34 3678 Fragments arranged by size: 3678 1421 1016 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3353 4109 5319 3353 Size: 756 1210 4251 Fragments arranged by size: 4251 1210 756 BclI T'GATC_A Cuts at: 1548 2739 1548 Size: 1191 5026 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2252 4828 136 Size: 107 122 71 631 605 580 2576 1525 Fragments arranged by size: 2576 1525 631 605 580 122 107 71 BglII A'GATC_T Cuts at: 2352 3204 3354 6212 2352 Size: 852 150 2858 2357 Fragments arranged by size: 2858 2357 852 150 BlpI GC'TnA_GC Cuts at: 3634 3634 Size: 6217 Bme1580I G_kGCm'C Cuts at: 1790 2284 2325 2688 2812 3489 4278 5434 1790 Size: 494 41 363 124 677 789 1156 2573 Fragments arranged by size: 2573 1156 789 677 494 363 124 41 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6217 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2015 2934 3948 Size: 416 53 83 399 24 598 919 1014 Cuts at: 3948 4906 442 Size: 958 1753 Fragments arranged by size: 1753 1014 958 919 598 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6217 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2383 2428 2680 3328 2383 Size: 45 252 648 5272 Fragments arranged by size: 5272 648 252 45 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2164 2589 2696 2792 2879 2924 2987 3079 Size: 925 425 107 96 87 45 63 92 Cuts at: 3079 3158 3323 3480 4877 1239 Size: 79 165 157 1397 2579 Fragments arranged by size: 2579 1397 925 425 165 157 107 96 92 87 79 63 45 Bpu10I CC'TnA_GC Cuts at: 2340 2474 3076 2340 Size: 134 602 5481 Fragments arranged by size: 5481 602 134 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4342 5207 5505 4342 Size: 865 298 5054 Fragments arranged by size: 5054 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4880 882 Size: 617 3381 2219 Fragments arranged by size: 3381 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4008 493 Size: 711 2804 2702 Fragments arranged by size: 2804 2702 711 BsaBI GATnn'nnATC Cuts at: 6211 6211 Size: 6217 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4024 4406 5041 275 Size: 53 83 186 936 2491 382 635 1451 Fragments arranged by size: 2491 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4650 5048 5322 5469 5880 4650 Size: 398 274 147 411 4987 Fragments arranged by size: 4987 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2765 2795 1178 Size: 30 165 30 1362 30 4600 Fragments arranged by size: 4600 1362 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2444 2447 2616 1186 Size: 1258 3 169 4787 Fragments arranged by size: 4787 1258 169 3 BseYI C'CCAG_C Cuts at: 2168 2394 2590 2612 2921 3423 3502 5420 5916 Size: 226 196 22 309 502 79 1918 496 Cuts at: 5916 2168 Size: 2469 Fragments arranged by size: 2469 1918 502 496 309 226 196 79 22 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2442 2513 1163 Size: 573 706 71 4867 Fragments arranged by size: 4867 706 573 71 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3667 4428 5456 1540 Size: 275 186 1666 761 1028 2301 Fragments arranged by size: 2301 1666 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3159 3405 4278 4363 5434 3159 Size: 246 873 85 1071 3942 Fragments arranged by size: 3942 1071 873 246 85 BsmI GAATG_Cn' Cuts at: 3728 3821 3728 Size: 93 6124 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2284 2325 2688 2812 2997 3159 3405 Size: 399 494 41 363 124 185 162 246 Cuts at: 3405 3489 3536 4278 4363 5434 1391 Size: 84 47 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 494 399 363 246 185 162 124 85 84 47 41 BspEI T'CCGG_A Cuts at: 5880 5880 Size: 6217 BspHI T'CATG_A Cuts at: 4106 5836 4106 Size: 1730 4487 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2280 2742 2866 3572 844 Size: 1436 462 124 706 3489 Fragments arranged by size: 3489 1436 706 462 124 BsrBI CCG'CTC Cuts at: 1394 1823 3063 4104 1394 Size: 429 1240 1041 3507 Fragments arranged by size: 3507 1240 1041 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4712 4886 65 Size: 1183 3464 174 1396 Fragments arranged by size: 3464 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2838 4861 5048 1700 Size: 110 1028 2023 187 2869 Fragments arranged by size: 2869 2023 1028 187 110 BsrGI T'GTAC_A Cuts at: 96 3268 96 Size: 3172 3045 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6217 BssSI C'ACGA_G Cuts at: 4277 5289 4277 Size: 1012 5205 BstAPI GCAn_nnn'nTGC Cuts at: 794 3433 794 Size: 2639 3578 BstBI TT'CG_AA Cuts at: 5021 5021 Size: 6217 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6217 BstXI CCAn_nnnn'nTGG Cuts at: 2023 2620 2902 2023 Size: 597 282 5338 Fragments arranged by size: 5338 597 282 BstYI r'GATC_y Cuts at: 1972 2352 3204 3354 3547 3914 4299 4316 5768 Size: 380 852 150 193 367 385 17 1452 Cuts at: 5768 5854 5866 6212 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 852 385 380 367 346 193 150 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2403 2928 2403 Size: 525 5692 BtgI C'CryG_G Cuts at: 513 1065 2583 2830 2989 513 Size: 552 1518 247 159 3741 Fragments arranged by size: 3741 1518 552 247 159 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5478 Fragments arranged by size: 5478 683 56 BtsI GCAGTG_nn' Cuts at: 2496 3733 4527 4547 2496 Size: 1237 794 20 4166 Fragments arranged by size: 4166 1237 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6217 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6182 DraI TTT'AAA Cuts at: 3521 3521 Size: 6217 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5224 809 Size: 507 3908 1802 Fragments arranged by size: 3908 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2253 2546 2798 Size: 54 1425 50 275 18 423 293 252 Cuts at: 2798 3478 3664 4553 6182 8 Size: 680 186 889 1629 43 Fragments arranged by size: 1629 1425 889 680 423 293 275 252 186 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3664 1537 Size: 275 1852 4090 Fragments arranged by size: 4090 1852 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2436 2442 2849 2891 4147 1353 Size: 1083 6 407 42 1256 3423 Fragments arranged by size: 3423 1256 1083 407 42 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2294 3023 4804 5178 5324 387 Size: 1064 138 705 729 1781 374 146 1280 Fragments arranged by size: 1781 1280 1064 729 705 374 146 138 EcoICRI GAG'CTC Cuts at: 3157 3403 3157 Size: 246 5971 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2075 2164 2589 2635 2696 2792 2879 Size: 393 443 89 425 46 61 96 87 Cuts at: 2879 2924 2987 3079 3158 3323 3480 4280 4877 Size: 45 63 92 79 165 157 800 597 Cuts at: 4877 5664 5932 1239 Size: 787 268 1524 Fragments arranged by size: 1524 800 787 597 443 425 393 268 165 157 96 92 89 87 79 63 61 46 45 EcoNI CCTnn'n_nnAGG Cuts at: 3462 3462 Size: 6217 EcoO109I rG'GnC_Cy Cuts at: 2322 2594 2671 2685 2809 3019 3451 3485 3486 Size: 272 77 14 124 210 432 34 1 Cuts at: 3486 2322 Size: 5053 Fragments arranged by size: 5053 432 272 210 124 77 34 14 1 EcoRI G'AATT_C Cuts at: 3526 3904 3526 Size: 378 5839 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2971 2971 Size: 6217 FspI TGC'GCA Cuts at: 4723 4723 Size: 6217 HaeII r_GCGC'y Cuts at: 1678 2956 5364 1678 Size: 1278 2408 2531 Fragments arranged by size: 2531 2408 1278 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4858 4890 4932 4964 1373 Size: 32 135 32 3286 32 42 32 2626 Fragments arranged by size: 3286 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2113 3561 3807 669 Size: 988 281 175 1448 246 3079 Fragments arranged by size: 3079 1448 988 281 246 175 HindIII A'AGCT_T Cuts at: 748 3125 748 Size: 2377 3840 HpaI GTT'AAC Cuts at: 3807 3807 Size: 6217 KpnI G_GTAC'C Cuts at: 3542 3542 Size: 6217 MfeI C'AATT_G Cuts at: 3816 3816 Size: 6217 MluI A'CGCG_T Cuts at: 6093 6093 Size: 6217 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5331 5515 1305 Size: 4026 184 2007 Fragments arranged by size: 4026 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3480 10 Size: 54 1425 343 1648 2747 Fragments arranged by size: 2747 1648 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2021 4175 4534 4693 518 Size: 648 855 2154 359 159 2042 Fragments arranged by size: 2154 2042 855 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2085 2857 2971 2991 3109 3335 3430 3506 Size: 479 772 114 20 118 226 95 76 Cuts at: 3506 3633 4310 5458 5703 1606 Size: 127 677 1148 245 2120 Fragments arranged by size: 2120 1148 772 677 479 245 226 127 118 114 95 76 20 NaeI GCC'GGC Cuts at: 2840 2840 Size: 6217 NcoI C'CATG_G Cuts at: 513 1065 2830 513 Size: 552 1765 3900 Fragments arranged by size: 3900 1765 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6217 NgoMIV G'CCGG_C Cuts at: 2838 2838 Size: 6217 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6217 NotI GC'GGCC_GC Cuts at: 3664 3664 Size: 6217 NspI r_CATG'y Cuts at: 1132 1229 2153 2335 2755 3514 3575 5120 1132 Size: 97 924 182 420 759 61 1545 2229 Fragments arranged by size: 2229 1545 924 759 420 182 97 61 PasI CC'CwG_GG Cuts at: 1348 2096 1348 Size: 748 5469 PciI A'CATG_T Cuts at: 5116 5116 Size: 6217 PflMI CCAn_nnn'nTGG Cuts at: 1108 2974 1108 Size: 1866 4351 PfoI T'CCnGG_A Cuts at: 2673 2982 3457 2673 Size: 309 475 5433 Fragments arranged by size: 5433 475 309 PmeI GTTT'AAAC Cuts at: 3521 3521 Size: 6217 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4286 4318 5799 5831 4286 Size: 32 1481 32 4672 Fragments arranged by size: 4672 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2594 2671 3019 2594 Size: 77 348 5792 Fragments arranged by size: 5792 348 77 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6217 PsiI TTA'TAA Cuts at: 3787 3787 Size: 6217 PspOMI G'GGCC_C Cuts at: 2321 2684 2808 3485 2321 Size: 363 124 677 5053 Fragments arranged by size: 5053 677 363 124 PstI C_TGCA'G Cuts at: 830 2457 2562 2577 2713 3569 830 Size: 1627 105 15 136 856 3478 Fragments arranged by size: 3478 1627 856 136 105 15 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6217 PvuII CAG'CTG Cuts at: 2085 2857 2971 3335 3506 2085 Size: 772 114 364 171 4796 Fragments arranged by size: 4796 772 364 171 114 SacI G_AGCT'C Cuts at: 3159 3405 3159 Size: 246 5971 SacII CC_GC'GG Cuts at: 2992 2992 Size: 6217 SalI G'TCGA_C Cuts at: 1936 3559 1936 Size: 1623 4594 SanDI GG'GwC_CC Cuts at: 2594 2594 Size: 6217 SapI GCTCTTCn'nnn_ Cuts at: 2442 2849 2442 Size: 407 5810 SbfI CC_TGCA'GG Cuts at: 2713 3569 2713 Size: 856 5361 ScaI AGT'ACT Cuts at: 4465 6177 4465 Size: 1712 4505 SfcI C'TryA_G Cuts at: 826 1045 2453 2558 2573 2709 2848 3008 3282 Size: 219 1408 105 15 136 139 160 274 Cuts at: 3282 3350 3565 4700 5381 5572 826 Size: 68 215 1135 681 191 1471 Fragments arranged by size: 1471 1408 1135 681 274 219 215 191 160 139 136 105 68 15 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6217 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6217 SmaI CCC'GGG Cuts at: 1872 2327 2807 3544 1872 Size: 455 480 737 4545 Fragments arranged by size: 4545 737 480 455 SmlI C'TyrA_G Cuts at: 820 1017 1943 4321 5222 5484 820 Size: 197 926 2378 901 262 1553 Fragments arranged by size: 2378 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4008 493 Size: 3515 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6217 SphI G_CATG'C Cuts at: 1132 2153 3514 3575 1132 Size: 1021 1361 61 3774 Fragments arranged by size: 3774 1361 1021 61 SspI AAT'ATT Cuts at: 5 52 4141 5 Size: 47 4089 2081 Fragments arranged by size: 4089 2081 47 StuI AGG'CCT Cuts at: 1517 2201 3468 1517 Size: 684 1267 4266 Fragments arranged by size: 4266 1267 684 StyI C'CwwG_G Cuts at: 513 1065 2208 2830 3469 3656 513 Size: 552 1143 622 639 187 3074 Fragments arranged by size: 3074 1143 639 622 552 187 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2780 2882 3516 4244 4261 4414 4599 Size: 1255 1200 102 634 728 17 153 185 Cuts at: 4599 5044 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1200 728 634 445 185 153 102 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3268 4463 6175 Size: 274 80 33 51 1443 1291 1195 1712 Cuts at: 6175 96 Size: 138 Fragments arranged by size: 1712 1443 1291 1195 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2528 2560 6177 6209 2528 Size: 32 3617 32 2536 Fragments arranged by size: 3617 2536 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6217 XbaI T'CTAG_A Cuts at: 3553 3553 Size: 6217 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2103 3455 2103 Size: 1352 4865 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6217 XmaI C'CCGG_G Cuts at: 1870 2325 2805 3542 1870 Size: 455 480 737 4545 Fragments arranged by size: 4545 737 480 455 XmnI GAAnn'nnTTC Cuts at: 2129 4346 2129 Size: 2217 4000 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4025 276 Size: 53 83 186 936 2491 2468 Fragments arranged by size: 2491 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I DraIII EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SexAI SfiI SfoI SrfI SwaI