Restriction map for
pFN21AB5892
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# NO CUT ENZYMES ( 138 enzymes) #
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AatII Acc65I AclI AflII AgeI
Aor51HI ApaI AscI AseI AsnI
Asp718I AspI AvaII AviII AvrII
BalI BamHI BanI BbeI BbrPI
BcgI BclI BfrI BglI BlnI
BlpI Bpu1102I BsaI BsgI BsiEI
BsiWI Bsp1407I BspDI BspHI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I CelII Cfr10I Cfr13I
ClaI CpoI DraI DraII DraIII
DrdI DsaI EaeI EagI EarI
EclXI Eco47III Eco52I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FbaI FseI FspI HpaI HphI
KasI KpnI Ksp632I KspI MfeI
MluI MluNI MscI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI Sau96I SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI StuI StyI
SwaI Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccI 73 GTCTAC GT^[AC][GT]AC
AccII 145 CGCG CG^CG
AccIII 774 TCCGGA T^CCGGA
AcyI 130 GACGCC G[GA]^CG[CT]C
AfaI 1024 GTAC GT^AC
AflIII 391 ACATGT A^C[GA][CT]GT
AhdI 1165 GACAACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1298 GTGCAC G^TGCAC
AocI 637 CCTCAGG CC^T[ACGT]AGG
ApaLI 1298 GTGCAC G^TGCAC
AspEI 1165 GACAACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1298 GTGCAC G[AT]GC[AT]^C
BanII 581 GAGCCC G[GA]GC[CT]^C
BbvCI 1249 CCTCAGC CCTCAGC(-5/-2)
BciVI 1046 GGATAC GTATCC(6/5)
BcnI 104 CCCGG CC^[GC]GG
BsaAI 1025 TACGTG [CT]AC^GT[GA]
BsaBI 613 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 130 GACGCC G[GA]^CG[CT]C
BsaWI 774 TCCGGA [AT]^CCGG[AT]
BseAI 774 TCCGGA T^CCGGA
BsiHKAI 1298 GTGCAC G[AT]GC[AT]^C
BsmBI 1411 GAGACG CGTCTC(1/5)
BspEI 774 TCCGGA T^CCGGA
BspLU11I 391 ACATGT A^CATGT
BstUI 145 CGCG CG^CG
Bsu36I 637 CCTCAGG CC^T[ACGT]AGG
CfoI 57 GCGC GCG^C
Eam1105I 1165 GACAACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 637 CCTCAGG CC^T[ACGT]AGG
FauI 995 GCGGG CCCGC(4/6)
HaeII 56 AGCGCC [GA]GCGC^[CT]
HaeIII 721 GGCC GG^CC
HgaI 130 GACGC GACGC(5/10)
HhaI 57 GCGC GCG^C
Hin1I 130 GACGCC G[GA]^CG[CT]C
HinP1I 57 GCGC G^CGC
HincII 1354 GTCAAC GT[CT]^[GA]AC
HindII 1354 GTCAAC GT[CT]^[GA]AC
MamI 613 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 774 TCCGGA T^CCGGA
MslI 1563 CACTTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 145 CGCG CG^CG
NciI 104 CCCGG CC^[GC]GG
NspI 391 ACATGT [GA]CATG^[CT]
RsaI 1024 GTAC GT^AC
SspI 436 AATATT AAT^ATT
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AlwI 1371 GATCC GGATC(4/5)
819 GGATC
AlwNI 898 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
1177 CAGCCCCTG
AvaI 82 CCCGAG C^[CT]CG[GA]G
154 CCCGAG
BbsI 521 GAAGAC GAAGAC(2/6)
1402 GAAGAC
BglII 89 AGATCT A^GATCT
600 AGATCT
BmyI 581 GAGCCC G[AGT]GC[ACT]^C
1298 GTGCAC
BpuAI 521 GAAGAC GAAGAC(2/6)
1402 GAAGAC
BseRI 85 GAGGAG GAGGAG(10/8)
115 GAGGAG
BsmFI 79 GTCCC GGGAC(10/14)
1173 GTCCC
BsmI 951 GAATGC GAATGC(1/-1)
1500 GAATGC
BsoBI 82 CCCGAG C^[CT]CG[GA]G
154 CCCGAG
Bsp1286I 581 GAGCCC G[AGT]GC[ACT]^C
1298 GTGCAC
BstYI 89 AGATCT [GA]^GATC[CT]
600 AGATCT
HapII 105 CCGG C^CGG
775 CCGG
HindIII 187 AAGCTT A^AGCTT
366 AAGCTT
HpaII 105 CCGG C^CGG
775 CCGG
MaeII 77 ACGT A^CGT
1026 ACGT
MflI 89 AGATCT [GA]^GATC[CT]
600 AGATCT
MspI 105 CCGG C^CGG
775 CCGG
TaqI 50 TCGA T^CGA
270 TCGA
TthHB8I 50 TCGA T^CGA
270 TCGA
XhoII 89 AGATCT [GA]^GATC[CT]
600 AGATCT
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# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AciI AcsI AluI ApoI Asp700I
BbvI BfaI BpmI BsaJI BsiYI
BslI BsmAI BspMI BsrI BssKI
BstF5I BstNI Cac8I DdeI DpnII
EcoRII Fnu4HI FokI HinfI ItaI
MaeI MaeIII MboI MboII MnlI
MseI MspA1I MvaI MwoI NdeII
NlaIII NlaIV PleI PstI PvuII
Sau3AI ScrFI SfaNI SfcI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XmnI