(Circular) (Six-base) MAPSORT of: pFN21AB5892.seq Check: 9700 from: 1 to: 6277 pFN21AB5892 6277 bp With 182 enzymes: SgfI * April 15, 2010 09:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4087 278 Size: 53 83 186 936 2551 2468 Fragments arranged by size: 2551 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2079 3620 1937 Size: 142 1541 4594 Fragments arranged by size: 4594 1541 142 Acc65I G'GTAC_C Cuts at: 3598 3598 Size: 6277 AclI AA'CG_TT Cuts at: 1563 4404 4777 1563 Size: 2841 373 3063 Fragments arranged by size: 3063 2841 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2323 2681 2733 2871 4340 5724 5992 1632 Size: 691 358 52 138 1469 1384 268 1917 Fragments arranged by size: 1917 1469 1384 691 358 268 138 52 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6080 AflIII A'CryG_T Cuts at: 1203 2396 5176 6153 1203 Size: 1193 2780 977 1327 Fragments arranged by size: 2780 1327 1193 977 AgeI A'CCGG_T Cuts at: 5108 5108 Size: 6277 AhdI GACnn_n'nnGTC Cuts at: 3175 5006 3175 Size: 1831 4446 AleI CACnn'nnGTG Cuts at: 1166 3572 1166 Size: 2406 3871 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6277 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3072 3104 3072 Size: 32 6245 AlwNI CAG_nnn'CTG Cuts at: 2908 3187 4020 5592 2908 Size: 279 833 1572 3593 Fragments arranged by size: 3593 1572 833 279 ApaLI G'TGCA_C Cuts at: 3303 4334 5490 3303 Size: 1031 1156 4090 Fragments arranged by size: 4090 1156 1031 ApoI r'AATT_y Cuts at: 1493 2214 2292 2389 3487 3586 3812 3964 6073 Size: 721 78 97 1098 99 226 152 2109 Cuts at: 6073 1493 Size: 1697 Fragments arranged by size: 2109 1697 1098 721 226 152 99 97 78 AseI AT'TA_AT Cuts at: 160 1032 4831 160 Size: 872 3799 1606 Fragments arranged by size: 3799 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6277 AvaI C'yCGr_G Cuts at: 1870 1943 2087 2159 3602 1870 Size: 73 144 72 1443 4545 Fragments arranged by size: 4545 1443 144 73 72 BamHI G'GATC_C Cuts at: 3607 3974 3607 Size: 367 5910 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3598 5053 618 Size: 325 217 627 1811 1455 1842 Fragments arranged by size: 1842 1811 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2590 3596 1391 Size: 1199 1006 4072 Fragments arranged by size: 4072 1199 1006 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2533 3414 928 Size: 971 634 881 3791 Fragments arranged by size: 3791 971 881 634 BbvCI CC'TCA_GC Cuts at: 3255 3255 Size: 6277 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4468 4502 1903 Size: 34 2531 34 3678 Fragments arranged by size: 3678 2531 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3045 4169 5379 3045 Size: 1124 1210 3943 Fragments arranged by size: 3943 1210 1124 BclI T'GATC_A Cuts at: 1548 1548 Size: 6277 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4888 136 Size: 107 122 71 631 605 3216 1525 Fragments arranged by size: 3216 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2094 2605 6272 2094 Size: 511 3667 2099 Fragments arranged by size: 3667 2099 511 BlpI GC'TnA_GC Cuts at: 3694 3694 Size: 6277 Bme1580I G_kGCm'C Cuts at: 1790 3307 4338 5494 1790 Size: 1517 1031 1156 2573 Fragments arranged by size: 2573 1517 1156 1031 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6277 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4008 4966 442 Size: 416 53 83 399 24 2591 958 1753 Fragments arranged by size: 2591 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6277 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2138 2214 3329 4937 1239 Size: 899 76 1115 1608 2579 Fragments arranged by size: 2579 1608 1115 899 76 Bpu10I CC'TnA_GC Cuts at: 3255 3255 Size: 6277 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4402 5267 5565 4402 Size: 865 298 5114 Fragments arranged by size: 5114 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4940 882 Size: 617 3441 2219 Fragments arranged by size: 3441 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3032 4068 493 Size: 711 1828 1036 2702 Fragments arranged by size: 2702 1828 1036 711 BsaBI GATnn'nnATC Cuts at: 2622 6271 2622 Size: 3649 2628 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2136 4084 4466 5101 Size: 53 83 186 936 603 1948 382 635 Cuts at: 5101 275 Size: 1451 Fragments arranged by size: 1948 1451 936 635 603 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2779 4710 5108 5382 5529 5940 2779 Size: 1931 398 274 147 411 3116 Fragments arranged by size: 3116 1931 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6052 Fragments arranged by size: 6052 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2105 2135 1186 Size: 919 30 5328 Fragments arranged by size: 5328 919 30 BseYI C'CCAG_C Cuts at: 2517 3180 5480 5976 2517 Size: 663 2300 496 2818 Fragments arranged by size: 2818 2300 663 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5704 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3727 4488 5516 1540 Size: 275 186 1726 761 1028 2301 Fragments arranged by size: 2301 1726 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3307 4338 4423 5494 3307 Size: 1031 85 1071 4090 Fragments arranged by size: 4090 1071 1031 85 BsmI GAATG_Cn' Cuts at: 2962 3511 3788 3881 2962 Size: 549 277 93 5358 Fragments arranged by size: 5358 549 277 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3410 3410 Size: 6277 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2590 3307 3596 4338 4423 5494 1391 Size: 399 800 717 289 742 85 1071 2174 Fragments arranged by size: 2174 1071 800 742 717 399 289 85 BspEI T'CCGG_A Cuts at: 2779 5940 2779 Size: 3161 3116 BspHI T'CATG_A Cuts at: 4166 5896 4166 Size: 1730 4547 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2317 2676 3064 3632 844 Size: 1473 359 388 568 3489 Fragments arranged by size: 3489 1473 568 388 359 BsrBI CCG'CTC Cuts at: 1394 1823 4164 1394 Size: 429 2341 3507 Fragments arranged by size: 3507 2341 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4772 4946 65 Size: 1183 3524 174 1396 Fragments arranged by size: 3524 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4921 5108 1700 Size: 110 3111 187 2869 Fragments arranged by size: 3111 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6277 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6277 BssSI C'ACGA_G Cuts at: 4337 5349 4337 Size: 1012 5265 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6277 BstBI TT'CG_AA Cuts at: 5081 5081 Size: 6277 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6277 BstXI CCAn_nnnn'nTGG Cuts at: 3574 3574 Size: 6277 BstYI r'GATC_y Cuts at: 1972 2094 2605 3607 3974 4359 4376 5828 5914 Size: 122 511 1002 367 385 17 1452 86 Cuts at: 5914 5926 6272 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1002 511 385 367 346 122 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2643 2643 Size: 6277 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5725 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5538 Fragments arranged by size: 5538 683 56 BtsI GCAGTG_nn' Cuts at: 2011 2065 2321 3793 4587 4607 2011 Size: 54 256 1472 794 20 3681 Fragments arranged by size: 3681 1472 794 256 54 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6277 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6242 DraI TTT'AAA Cuts at: 3581 3581 Size: 6277 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5284 809 Size: 507 3968 1802 Fragments arranged by size: 3968 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3724 4613 6242 Size: 54 1425 50 275 18 1894 889 1629 Cuts at: 6242 8 Size: 43 Fragments arranged by size: 1894 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3724 1537 Size: 275 1912 4090 Fragments arranged by size: 4090 1912 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4207 1353 Size: 2854 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4864 5238 5384 387 Size: 1064 138 3275 374 146 1280 Fragments arranged by size: 3275 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2138 2214 2323 2681 2733 2871 3329 Size: 393 506 76 109 358 52 138 458 Cuts at: 3329 4340 4937 5724 5992 1239 Size: 1011 597 787 268 1524 Fragments arranged by size: 1524 1011 787 597 506 458 393 358 268 138 109 76 52 EcoRI G'AATT_C Cuts at: 3586 3964 3586 Size: 378 5899 FspI TGC'GCA Cuts at: 4783 4783 Size: 6277 HaeII r_GCGC'y Cuts at: 1678 2065 5424 1678 Size: 387 3359 2531 Fragments arranged by size: 3359 2531 387 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2470 2502 3161 3193 4918 Size: 32 135 32 898 32 659 32 1725 Cuts at: 4918 4950 4992 5024 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1725 898 659 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3361 3621 3867 669 Size: 988 281 1423 260 246 3079 Fragments arranged by size: 3079 1423 988 281 260 246 HindIII A'AGCT_T Cuts at: 748 2192 2371 748 Size: 1444 179 4654 Fragments arranged by size: 4654 1444 179 HpaI GTT'AAC Cuts at: 3867 3867 Size: 6277 KpnI G_GTAC'C Cuts at: 3602 3602 Size: 6277 MfeI C'AATT_G Cuts at: 3876 3876 Size: 6277 MluI A'CGCG_T Cuts at: 6153 6153 Size: 6277 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2563 2595 2679 5391 5575 1305 Size: 1258 32 84 2712 184 2007 Fragments arranged by size: 2712 2007 1258 184 84 32 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4455 Fragments arranged by size: 4455 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3572 4235 4594 4753 518 Size: 648 2406 663 359 159 2042 Fragments arranged by size: 2406 2042 663 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2221 2254 2343 2521 3259 3693 4370 5518 Size: 615 33 89 178 738 434 677 1148 Cuts at: 5518 5763 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 738 677 615 434 245 178 89 33 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5725 NdeI CA'TA_TG Cuts at: 387 387 Size: 6277 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6277 NotI GC'GGCC_GC Cuts at: 3724 3724 Size: 6277 NspI r_CATG'y Cuts at: 1132 1229 2400 3635 5180 1132 Size: 97 1171 1235 1545 2229 Fragments arranged by size: 2229 1545 1235 1171 97 PasI CC'CwG_GG Cuts at: 1348 3452 1348 Size: 2104 4173 PciI A'CATG_T Cuts at: 2396 5176 2396 Size: 2780 3497 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6277 PfoI T'CCnGG_A Cuts at: 3090 3090 Size: 6277 PmeI GTTT'AAAC Cuts at: 3581 3581 Size: 6277 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4346 4378 5859 5891 4346 Size: 32 1481 32 4732 Fragments arranged by size: 4732 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6277 PsiI TTA'TAA Cuts at: 2457 3847 2457 Size: 1390 4887 PstI C_TGCA'G Cuts at: 830 2328 3011 3061 3629 830 Size: 1498 683 50 568 3478 Fragments arranged by size: 3478 1498 683 568 50 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6277 PvuII CAG'CTG Cuts at: 2254 2521 3259 2254 Size: 267 738 5272 Fragments arranged by size: 5272 738 267 SalI G'TCGA_C Cuts at: 1936 3619 1936 Size: 1683 4594 SbfI CC_TGCA'GG Cuts at: 3629 3629 Size: 6277 ScaI AGT'ACT Cuts at: 4525 6237 4525 Size: 1712 4565 SfcI C'TryA_G Cuts at: 826 1045 2324 2423 3007 3057 3625 4760 5441 Size: 219 1279 99 584 50 568 1135 681 Cuts at: 5441 5632 826 Size: 191 1471 Fragments arranged by size: 1471 1279 1135 681 584 568 219 191 99 50 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6277 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6277 SmaI CCC'GGG Cuts at: 1872 3604 1872 Size: 1732 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4381 5282 5544 820 Size: 197 926 2438 901 262 1553 Fragments arranged by size: 2438 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4068 493 Size: 3575 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6277 SphI G_CATG'C Cuts at: 1132 3635 1132 Size: 2503 3774 SspI AAT'ATT Cuts at: 5 52 2443 4201 5 Size: 47 2391 1758 2081 Fragments arranged by size: 2391 2081 1758 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6277 StyI C'CwwG_G Cuts at: 513 1065 3716 513 Size: 552 2651 3074 Fragments arranged by size: 3074 2651 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3465 3573 4304 4321 4474 4659 5104 Size: 1255 1885 108 731 17 153 185 445 Cuts at: 5104 325 Size: 1498 Fragments arranged by size: 1885 1498 1255 731 445 185 153 108 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4523 6235 96 Size: 274 80 33 51 1443 2546 1712 138 Fragments arranged by size: 2546 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3337 3369 6237 6269 3337 Size: 32 2868 32 3345 Fragments arranged by size: 3345 2868 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6277 XbaI T'CTAG_A Cuts at: 3613 3613 Size: 6277 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6277 XmaI C'CCGG_G Cuts at: 1870 3602 1870 Size: 1732 4545 XmnI GAAnn'nnTTC Cuts at: 2373 2706 2775 4406 2373 Size: 333 69 1631 4244 Fragments arranged by size: 4244 1631 333 69 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4085 276 Size: 53 83 186 936 2551 2468 Fragments arranged by size: 2551 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI