Restriction map for
pFN21AB5892
(Linear) (Six-base) MAPSORT of: KIBB5892.seq Check: 4558 from: 1 to: 1572
KIBB5892.orf
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
AccI GT'mk_AC
Cuts at: 0 74 1572
Size: 74 1498
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 318 676 728 866 1572
Size: 318 358 52 138 706
Fragments arranged by size:
706 358 318 138 52
AflIII A'CryG_T
Cuts at: 0 391 1572
Size: 391 1181
AhdI GACnn_n'nnGTC
Cuts at: 0 1170 1572
Size: 1170 402
AleI CACnn'nnGTG
Cuts at: 0 1567 1572
Size: 1567 5
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1067 1099 1572
Size: 1067 32 473
Fragments arranged by size:
1067 473 32
AlwNI CAG_nnn'CTG
Cuts at: 0 903 1182 1572
Size: 903 279 390
Fragments arranged by size:
903 390 279
ApaLI G'TGCA_C
Cuts at: 0 1298 1572
Size: 1298 274
ApoI r'AATT_y
Cuts at: 0 209 287 384 1482 1572
Size: 209 78 97 1098 90
Fragments arranged by size:
1098 209 97 90 78
AvaI C'yCGr_G
Cuts at: 0 82 154 1572
Size: 82 72 1418
Fragments arranged by size:
1418 82 72
BanII G_rGCy'C
Cuts at: 0 585 1572
Size: 585 987
BbsI GAAGACnn'nnnn_
Cuts at: 0 528 1409 1572
Size: 528 881 163
Fragments arranged by size:
881 528 163
BbvCI CC'TCA_GC
Cuts at: 0 1250 1572
Size: 1250 322
BciVI GTATCCnnnnn_n'
Cuts at: 0 1040 1572
Size: 1040 532
BglII A'GATC_T
Cuts at: 0 89 600 1572
Size: 89 511 972
Fragments arranged by size:
972 511 89
Bme1580I G_kGCm'C
Cuts at: 0 1302 1572
Size: 1302 270
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 133 209 1324 1572
Size: 133 76 1115 248
Fragments arranged by size:
1115 248 133 76
Bpu10I CC'TnA_GC
Cuts at: 0 1250 1572
Size: 1250 322
BsaAI yAC'GTr
Cuts at: 0 1027 1572
Size: 1027 545
BsaBI GATnn'nnATC
Cuts at: 0 617 1572
Size: 617 955
BsaHI Gr'CG_yC
Cuts at: 0 131 1572
Size: 131 1441
BsaWI w'CCGG_w
Cuts at: 0 774 1572
Size: 774 798
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 100 130 1572
Size: 100 30 1442
Fragments arranged by size:
1442 100 30
BseYI C'CCAG_C
Cuts at: 0 512 1175 1572
Size: 512 663 397
Fragments arranged by size:
663 512 397
BsiHKAI G_wGCw'C
Cuts at: 0 1302 1572
Size: 1302 270
BsmI GAATG_Cn'
Cuts at: 0 957 1506 1572
Size: 957 549 66
Fragments arranged by size:
957 549 66
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1405 1572
Size: 1405 167
Bsp1286I G_dGCh'C
Cuts at: 0 585 1302 1572
Size: 585 717 270
Fragments arranged by size:
717 585 270
BspEI T'CCGG_A
Cuts at: 0 774 1572
Size: 774 798
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 312 671 1059 1572
Size: 312 359 388 513
Fragments arranged by size:
513 388 359 312
BstYI r'GATC_y
Cuts at: 0 89 600 1572
Size: 89 511 972
Fragments arranged by size:
972 511 89
Bsu36I CC'TnA_GG
Cuts at: 0 638 1572
Size: 638 934
BtsI GCAGTG_nn'
Cuts at: 0 6 60 316 1572
Size: 6 54 256 1256
Fragments arranged by size:
1256 256 54 6
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 133 209 318 676 728 866 1324 1572
Size: 133 76 109 358 52 138 458 248
Fragments arranged by size:
458 358 248 138 133 109 76 52
HaeII r_GCGC'y
Cuts at: 0 60 1572
Size: 60 1512
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 465 497 1156 1188 1572
Size: 465 32 659 32 384
Fragments arranged by size:
659 465 384 32 32
HincII GTy'rAC
Cuts at: 0 1356 1572
Size: 1356 216
HindIII A'AGCT_T
Cuts at: 0 187 366 1572
Size: 187 179 1206
Fragments arranged by size:
1206 187 179
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 558 590 674 1572
Size: 558 32 84 898
Fragments arranged by size:
898 558 84 32
MslI CAynn'nnrTG
Cuts at: 0 1567 1572
Size: 1567 5
MspA1I CmG'CkG
Cuts at: 0 216 249 338 516 1254 1572
Size: 216 33 89 178 738 318
Fragments arranged by size:
738 318 216 178 89 33
NspI r_CATG'y
Cuts at: 0 395 1572
Size: 395 1177
PasI CC'CwG_GG
Cuts at: 0 1447 1572
Size: 1447 125
PciI A'CATG_T
Cuts at: 0 391 1572
Size: 391 1181
PfoI T'CCnGG_A
Cuts at: 0 1085 1572
Size: 1085 487
PsiI TTA'TAA
Cuts at: 0 452 1572
Size: 452 1120
PstI C_TGCA'G
Cuts at: 0 323 1006 1056 1572
Size: 323 683 50 516
Fragments arranged by size:
683 516 323 50
PvuII CAG'CTG
Cuts at: 0 249 516 1254 1572
Size: 249 267 738 318
Fragments arranged by size:
738 318 267 249
SfcI C'TryA_G
Cuts at: 0 319 418 1002 1052 1572
Size: 319 99 584 50 520
Fragments arranged by size:
584 520 319 99 50
SspI AAT'ATT
Cuts at: 0 438 1572
Size: 438 1134
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1460 1568 1572
Size: 1460 108 4
Fragments arranged by size:
1460 108 4
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1332 1364 1572
Size: 1332 32 208
Fragments arranged by size:
1332 208 32
XmnI GAAnn'nnTTC
Cuts at: 0 368 701 770 1572
Size: 368 333 69 802
Fragments arranged by size:
802 368 333 69
Enzymes that do cut:
AccI AcuI AflIII AhdI AleI AloI AlwNI ApaLI
ApoI AvaI BanII BbsI BbvCI BciVI BglII Bme1580I
BpmI Bpu10I BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI BstYI Bsu36I
BtsI Eco57MI HaeII Hin4I HincII HindIII MmeI MslI
MspA1I NspI PasI PciI PfoI PsiI PstI PvuII
SfcI SspI TaqII TstI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AlfI
ApaI AscI AseI AsiSI AvrII BaeI BamHI BanI
BbeI BcgI BclI BfrBI BglI BlpI BmgBI BmrI
BmtI BplI BpuEI BsaI BsaXI BsgI BsiEI BsiWI
BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstZ17I BtgI BtgZI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI
HpaI KasI KpnI MfeI MluI MscI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI
PflMI PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI StuI StyI SwaI
TatI Tth111I XbaI XcmI XhoI XmaI ZraI