(Circular) (Six-base) MAPSORT of: pFN21AB5880.seq Check: 4894 from: 1 to: 6193 pFN21AB5880 6193 bp With 182 enzymes: SgfI * April 15, 2010 09:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2891 2891 Size: 6193 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4003 278 Size: 53 83 186 936 2467 2468 Fragments arranged by size: 2468 2467 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3536 1937 Size: 1599 4594 Acc65I G'GTAC_C Cuts at: 3514 3514 Size: 6193 AclI AA'CG_TT Cuts at: 1563 3276 4320 4693 1563 Size: 1713 1044 373 3063 Fragments arranged by size: 3063 1713 1044 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4256 5640 5908 1632 Size: 2624 1384 268 1917 Fragments arranged by size: 2624 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5996 AflIII A'CryG_T Cuts at: 1203 2562 5092 6069 1203 Size: 1359 2530 977 1327 Fragments arranged by size: 2530 1359 1327 977 AgeI A'CCGG_T Cuts at: 5024 5024 Size: 6193 AhdI GACnn_n'nnGTC Cuts at: 4922 4922 Size: 6193 AleI CACnn'nnGTG Cuts at: 1166 3158 1166 Size: 1992 4201 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6193 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3110 3142 3110 Size: 32 6161 AlwNI CAG_nnn'CTG Cuts at: 3936 5508 3936 Size: 1572 4621 ApaLI G'TGCA_C Cuts at: 3050 4250 5406 3050 Size: 1200 1156 3837 Fragments arranged by size: 3837 1200 1156 ApoI r'AATT_y Cuts at: 1493 3502 3728 3880 5989 1493 Size: 2009 226 152 2109 1697 Fragments arranged by size: 2109 2009 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4747 160 Size: 872 3715 1606 Fragments arranged by size: 3715 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6193 AvaI C'yCGr_G Cuts at: 1870 1943 3518 1870 Size: 73 1575 4545 Fragments arranged by size: 4545 1575 73 BamHI G'GATC_C Cuts at: 2268 3523 3890 2268 Size: 1255 367 4571 Fragments arranged by size: 4571 1255 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2041 2879 3514 4969 618 Size: 325 217 627 254 838 635 1455 1842 Fragments arranged by size: 1842 1455 838 635 627 325 254 217 BanII G_rGCy'C Cuts at: 1391 2451 2605 3512 1391 Size: 1060 154 907 4072 Fragments arranged by size: 4072 1060 907 154 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5222 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4384 4418 1903 Size: 34 2447 34 3678 Fragments arranged by size: 3678 2447 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2359 4085 5295 2359 Size: 1726 1210 3257 Fragments arranged by size: 3257 1726 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6193 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4804 136 Size: 107 122 71 631 605 3132 1525 Fragments arranged by size: 3132 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6188 6188 Size: 6193 BlpI GC'TnA_GC Cuts at: 3610 3610 Size: 6193 Bme1580I G_kGCm'C Cuts at: 1790 3054 4254 5410 1790 Size: 1264 1200 1156 2573 Fragments arranged by size: 2573 1264 1200 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6193 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2623 3924 4882 Size: 416 53 83 399 24 1206 1301 958 Cuts at: 4882 442 Size: 1753 Fragments arranged by size: 1753 1301 1206 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2343 1056 Size: 1287 4906 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2317 2799 2317 Size: 482 5711 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2181 2769 2843 3410 4853 1239 Size: 942 588 74 567 1443 2579 Fragments arranged by size: 2579 1443 942 588 567 74 Bpu10I CC'TnA_GC Cuts at: 2945 3197 2945 Size: 252 5941 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2641 3383 4318 5183 5481 2641 Size: 742 935 865 298 3353 Fragments arranged by size: 3353 935 865 742 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2659 4856 882 Size: 617 1160 2197 2219 Fragments arranged by size: 2219 2197 1160 617 BsaAI yAC'GTr Cuts at: 493 1204 3984 493 Size: 711 2780 2702 Fragments arranged by size: 2780 2702 711 BsaBI GATnn'nnATC Cuts at: 6187 6187 Size: 6193 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3315 4000 4382 5017 Size: 53 83 186 936 1782 685 382 635 Cuts at: 5017 275 Size: 1451 Fragments arranged by size: 1782 1451 936 685 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4626 5024 5298 5445 5856 4626 Size: 398 274 147 411 4963 Fragments arranged by size: 4963 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3110 3140 1178 Size: 30 165 30 1707 30 4231 Fragments arranged by size: 4231 1707 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2126 2348 3050 3125 1186 Size: 940 222 702 75 4254 Fragments arranged by size: 4254 940 702 222 75 BseYI C'CCAG_C Cuts at: 2386 2692 3011 3455 5396 5892 2386 Size: 306 319 444 1941 496 2687 Fragments arranged by size: 2687 1941 496 444 319 306 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5620 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2500 3643 4404 5432 1540 Size: 275 186 499 1143 761 1028 2301 Fragments arranged by size: 2301 1143 1028 761 499 275 186 BsiHKAI G_wGCw'C Cuts at: 2091 3054 3123 4254 4339 5410 2091 Size: 963 69 1131 85 1071 2874 Fragments arranged by size: 2874 1131 1071 963 85 69 BsmI GAATG_Cn' Cuts at: 3704 3797 3704 Size: 93 6100 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2091 2451 2605 3054 3123 3512 4254 Size: 399 301 360 154 449 69 389 742 Cuts at: 4254 4339 5410 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 449 399 389 360 301 154 85 69 BspEI T'CCGG_A Cuts at: 5856 5856 Size: 6193 BspHI T'CATG_A Cuts at: 3099 3225 3306 4082 5812 3099 Size: 126 81 776 1730 3480 Fragments arranged by size: 3480 1730 776 126 81 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2326 2877 2891 3432 3548 844 Size: 1482 551 14 541 116 3489 Fragments arranged by size: 3489 1482 551 541 116 14 BsrBI CCG'CTC Cuts at: 1394 1823 4080 1394 Size: 429 2257 3507 Fragments arranged by size: 3507 2257 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4688 4862 65 Size: 1183 3440 174 1396 Fragments arranged by size: 3440 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2038 4837 5024 1700 Size: 110 228 2799 187 2869 Fragments arranged by size: 2869 2799 228 187 110 BsrGI T'GTAC_A Cuts at: 96 2520 96 Size: 2424 3769 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6193 BssSI C'ACGA_G Cuts at: 4253 5265 4253 Size: 1012 5181 BstAPI GCAn_nnn'nTGC Cuts at: 794 2100 794 Size: 1306 4887 BstBI TT'CG_AA Cuts at: 4997 4997 Size: 6193 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6193 BstXI CCAn_nnnn'nTGG Cuts at: 2329 2619 2329 Size: 290 5903 BstYI r'GATC_y Cuts at: 1972 2268 3364 3523 3890 4275 4292 5744 5830 Size: 296 1096 159 367 385 17 1452 86 Cuts at: 5830 5842 6188 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1096 385 367 346 296 159 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2328 2407 2415 2912 513 Size: 552 939 324 79 8 497 3794 Fragments arranged by size: 3794 939 552 497 324 79 8 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5454 Fragments arranged by size: 5454 683 56 BtsI GCAGTG_nn' Cuts at: 2034 2089 2215 2847 3709 4503 4523 2034 Size: 55 126 632 862 794 20 3704 Fragments arranged by size: 3704 862 794 632 126 55 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6193 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2116 2151 2901 2936 529 Size: 35 1552 35 750 35 3786 Fragments arranged by size: 3786 1552 750 35 35 35 DraI TTT'AAA Cuts at: 3497 3497 Size: 6193 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3320 5200 809 Size: 507 2004 1880 1802 Fragments arranged by size: 2004 1880 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2080 2609 3640 Size: 54 1425 50 275 18 250 529 1031 Cuts at: 3640 4529 6158 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1031 889 529 275 250 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3640 1537 Size: 275 1828 4090 Fragments arranged by size: 4090 1828 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3300 4123 1353 Size: 1947 823 3423 Fragments arranged by size: 3423 1947 823 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2024 2429 4780 5154 5300 387 Size: 1064 138 435 405 2351 374 146 1280 Fragments arranged by size: 2351 1280 1064 435 405 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2181 2769 2843 3410 4256 4853 5640 Size: 393 549 588 74 567 846 597 787 Cuts at: 5640 5908 1239 Size: 268 1524 Fragments arranged by size: 1524 846 787 597 588 567 549 393 268 74 EcoNI CCTnn'n_nnAGG Cuts at: 2462 2462 Size: 6193 EcoO109I rG'GnC_Cy Cuts at: 2315 2315 Size: 6193 EcoRI G'AATT_C Cuts at: 3502 3880 3502 Size: 378 5815 FspI TGC'GCA Cuts at: 4699 4699 Size: 6193 HaeII r_GCGC'y Cuts at: 1678 2688 5340 1678 Size: 1010 2652 2531 Fragments arranged by size: 2652 2531 1010 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4834 4866 4908 4940 1373 Size: 32 135 32 3262 32 42 32 2626 Fragments arranged by size: 3262 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3537 3783 669 Size: 988 281 1599 246 3079 Fragments arranged by size: 3079 1599 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6193 HpaI GTT'AAC Cuts at: 3783 3783 Size: 6193 KpnI G_GTAC'C Cuts at: 3518 3518 Size: 6193 MfeI C'AATT_G Cuts at: 3442 3792 3442 Size: 350 5843 MluI A'CGCG_T Cuts at: 6069 6069 Size: 6193 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2642 3260 3457 5307 5491 1305 Size: 1337 618 197 1850 184 2007 Fragments arranged by size: 2007 1850 1337 618 197 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4371 Fragments arranged by size: 4371 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2709 3158 3224 4151 4510 4669 518 Size: 648 1543 449 66 927 359 159 2042 Fragments arranged by size: 2042 1543 927 648 449 359 159 66 MspA1I CmG'CkG Cuts at: 1606 2050 3011 3609 4286 5434 5679 1606 Size: 444 961 598 677 1148 245 2120 Fragments arranged by size: 2120 1148 961 677 598 444 245 NaeI GCC'GGC Cuts at: 2040 2040 Size: 6193 NcoI C'CATG_G Cuts at: 513 1065 2004 2328 2407 513 Size: 552 939 324 79 4299 Fragments arranged by size: 4299 939 552 324 79 NdeI CA'TA_TG Cuts at: 387 387 Size: 6193 NgoMIV G'CCGG_C Cuts at: 2038 2038 Size: 6193 NheI G'CTAG_C Cuts at: 1052 2339 1052 Size: 1287 4906 NotI GC'GGCC_GC Cuts at: 3640 3640 Size: 6193 NspI r_CATG'y Cuts at: 1132 1229 2566 3551 5096 1132 Size: 97 1337 985 1545 2229 Fragments arranged by size: 2229 1545 1337 985 97 PasI CC'CwG_GG Cuts at: 1348 2464 1348 Size: 1116 5077 PciI A'CATG_T Cuts at: 2562 5092 2562 Size: 2530 3663 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6193 PfoI T'CCnGG_A Cuts at: 2271 3387 2271 Size: 1116 5077 PmeI GTTT'AAAC Cuts at: 3497 3497 Size: 6193 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3110 3142 4262 4294 5775 5807 3110 Size: 32 1120 32 1481 32 3496 Fragments arranged by size: 3496 1481 1120 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2315 2315 Size: 6193 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6193 PsiI TTA'TAA Cuts at: 3763 3763 Size: 6193 PstI C_TGCA'G Cuts at: 830 2096 2888 3429 3545 830 Size: 1266 792 541 116 3478 Fragments arranged by size: 3478 1266 792 541 116 PvuI CG_AT'CG Cuts at: 2001 2500 2001 Size: 499 5694 PvuII CAG'CTG Cuts at: 3011 3011 Size: 6193 SalI G'TCGA_C Cuts at: 1936 3535 1936 Size: 1599 4594 SbfI CC_TGCA'GG Cuts at: 2888 3545 2888 Size: 657 5536 ScaI AGT'ACT Cuts at: 4441 6153 4441 Size: 1712 4481 SexAI A'CCwGG_T Cuts at: 3065 3065 Size: 6193 SfcI C'TryA_G Cuts at: 826 1045 2092 2884 3425 3541 4676 5357 5548 Size: 219 1047 792 541 116 1135 681 191 Cuts at: 5548 826 Size: 1471 Fragments arranged by size: 1471 1135 1047 792 681 541 219 191 116 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6193 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6193 SmaI CCC'GGG Cuts at: 1872 3520 1872 Size: 1648 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2656 3398 4297 5198 5460 820 Size: 197 926 713 742 899 901 262 1553 Fragments arranged by size: 1553 926 901 899 742 713 262 197 SnaBI TAC'GTA Cuts at: 493 3984 493 Size: 3491 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6193 SphI G_CATG'C Cuts at: 1132 3551 1132 Size: 2419 3774 SspI AAT'ATT Cuts at: 5 52 4117 5 Size: 47 4065 2081 Fragments arranged by size: 4065 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6193 StyI C'CwwG_G Cuts at: 513 1065 2004 2328 2407 3632 513 Size: 552 939 324 79 1225 3074 Fragments arranged by size: 3074 1225 939 552 324 79 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2420 2889 3299 4220 4237 4390 4575 Size: 1255 840 469 410 921 17 153 185 Cuts at: 4575 5020 325 Size: 445 1498 Fragments arranged by size: 1498 1255 921 840 469 445 410 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2520 4439 6151 Size: 274 80 33 51 1443 543 1919 1712 Cuts at: 6151 96 Size: 138 Fragments arranged by size: 1919 1712 1443 543 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6153 6185 6153 Size: 32 6161 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6193 XbaI T'CTAG_A Cuts at: 3298 3529 3298 Size: 231 5962 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2636 2636 Size: 6193 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6193 XmaI C'CCGG_G Cuts at: 1870 3518 1870 Size: 1648 4545 XmnI GAAnn'nnTTC Cuts at: 4322 4322 Size: 6193 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4001 276 Size: 53 83 186 936 2467 2468 Fragments arranged by size: 2468 2467 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI