(Circular) (Six-base) MAPSORT of: pFN21AB5855.seq Check: 344 from: 1 to: 6082 pFN21AB5855 6082 bp With 182 enzymes: SgfI * April 15, 2010 09:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3892 278 Size: 53 83 186 936 2356 2468 Fragments arranged by size: 2468 2356 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3425 1937 Size: 1488 4594 Acc65I G'GTAC_C Cuts at: 3098 3403 3098 Size: 305 5777 AclI AA'CG_TT Cuts at: 1563 4209 4582 1563 Size: 2646 373 3063 Fragments arranged by size: 3063 2646 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2079 2192 2543 2783 4145 5529 5797 1632 Size: 447 113 351 240 1362 1384 268 1917 Fragments arranged by size: 1917 1384 1362 447 351 268 240 113 AfeI AGC'GCT Cuts at: 3269 3269 Size: 6082 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5885 AflIII A'CryG_T Cuts at: 1203 4981 5958 1203 Size: 3778 977 1327 Fragments arranged by size: 3778 1327 977 AgeI A'CCGG_T Cuts at: 4913 4913 Size: 6082 AhdI GACnn_n'nnGTC Cuts at: 4811 4811 Size: 6082 AleI CACnn'nnGTG Cuts at: 1166 2201 2949 1166 Size: 1035 748 4299 Fragments arranged by size: 4299 1035 748 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2357 2826 1184 Size: 1173 469 4440 Fragments arranged by size: 4440 1173 469 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3274 3306 3274 Size: 32 6050 AlwNI CAG_nnn'CTG Cuts at: 2305 2651 3118 3825 5397 2305 Size: 346 467 707 1572 2990 Fragments arranged by size: 2990 1572 707 467 346 ApaI G_GGCC'C Cuts at: 3138 3231 3138 Size: 93 5989 ApaLI G'TGCA_C Cuts at: 4139 5295 4139 Size: 1156 4926 ApoI r'AATT_y Cuts at: 1493 2373 3391 3617 3769 5878 1493 Size: 880 1018 226 152 2109 1697 Fragments arranged by size: 2109 1697 1018 880 226 152 AseI AT'TA_AT Cuts at: 160 1032 4636 160 Size: 872 3604 1606 Fragments arranged by size: 3604 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6082 AvaI C'yCGr_G Cuts at: 1870 1943 2262 3246 3312 3407 1870 Size: 73 319 984 66 95 4545 Fragments arranged by size: 4545 984 319 95 73 66 BamHI G'GATC_C Cuts at: 2852 3412 3779 2852 Size: 560 367 5155 Fragments arranged by size: 5155 560 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2236 3063 3098 3403 4858 Size: 325 217 627 449 827 35 305 1455 Cuts at: 4858 618 Size: 1842 Fragments arranged by size: 1842 1455 827 627 449 325 305 217 35 BanII G_rGCy'C Cuts at: 1391 2616 3138 3150 3231 3401 1391 Size: 1225 522 12 81 170 4072 Fragments arranged by size: 4072 1225 522 170 81 12 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5111 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4273 4307 1903 Size: 34 2336 34 3678 Fragments arranged by size: 3678 2336 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2235 3974 5184 2235 Size: 1739 1210 3133 Fragments arranged by size: 3133 1739 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6082 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4693 136 Size: 107 122 71 631 605 337 2684 1525 Fragments arranged by size: 2684 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 2136 2244 6077 2136 Size: 108 3833 2141 Fragments arranged by size: 3833 2141 108 BlpI GC'TnA_GC Cuts at: 2772 3499 2772 Size: 727 5355 Bme1580I G_kGCm'C Cuts at: 1790 2336 3138 3231 4143 5299 1790 Size: 546 802 93 912 1156 2573 Fragments arranged by size: 2573 1156 912 802 546 93 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6082 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2053 2679 3024 Size: 416 53 83 399 24 636 626 345 Cuts at: 3024 3813 4771 442 Size: 789 958 1753 Fragments arranged by size: 1753 958 789 636 626 416 399 345 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6082 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2633 2633 Size: 6082 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4742 1239 Size: 3503 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2786 3125 4207 5072 5370 2786 Size: 339 1082 865 298 3498 Fragments arranged by size: 3498 1082 865 339 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4745 882 Size: 617 3246 2219 Fragments arranged by size: 3246 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2314 3873 493 Size: 711 1110 1559 2702 Fragments arranged by size: 2702 1559 1110 711 BsaBI GATnn'nnATC Cuts at: 2141 2249 6076 2141 Size: 108 3827 2147 Fragments arranged by size: 3827 2147 108 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2012 3889 4271 4906 Size: 53 83 186 936 479 1877 382 635 Cuts at: 4906 275 Size: 1451 Fragments arranged by size: 1877 1451 936 635 479 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4515 4913 5187 5334 5745 4515 Size: 398 274 147 411 4852 Fragments arranged by size: 4852 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5857 Fragments arranged by size: 5857 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2051 2913 1186 Size: 865 862 4355 Fragments arranged by size: 4355 865 862 BseYI C'CCAG_C Cuts at: 2215 2305 2324 2329 2864 2940 3261 5285 5781 Size: 90 19 5 535 76 321 2024 496 Cuts at: 5781 2215 Size: 2516 Fragments arranged by size: 2516 2024 535 496 321 90 76 19 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2612 2626 2709 1163 Size: 573 876 14 83 4536 Fragments arranged by size: 4536 876 573 83 14 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3532 4293 5321 1540 Size: 275 186 1531 761 1028 2301 Fragments arranged by size: 2301 1531 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4143 4228 5299 4143 Size: 85 1071 4926 Fragments arranged by size: 4926 1071 85 BsmI GAATG_Cn' Cuts at: 3593 3686 3593 Size: 93 5989 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2336 2616 3138 3150 3231 3401 4143 Size: 399 546 280 522 12 81 170 742 Cuts at: 4143 4228 5299 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 546 522 399 280 170 85 81 12 BspEI T'CCGG_A Cuts at: 5745 5745 Size: 6082 BspHI T'CATG_A Cuts at: 3971 5701 3971 Size: 1730 4352 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3437 844 Size: 2593 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2746 3969 1394 Size: 429 923 1223 3507 Fragments arranged by size: 3507 1223 923 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4577 4751 65 Size: 1183 3329 174 1396 Fragments arranged by size: 3329 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3330 4726 4913 1700 Size: 110 1520 1396 187 2869 Fragments arranged by size: 2869 1520 1396 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6082 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6082 BssSI C'ACGA_G Cuts at: 2618 4142 5154 2618 Size: 1524 1012 3546 Fragments arranged by size: 3546 1524 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6082 BstBI TT'CG_AA Cuts at: 4886 4886 Size: 6082 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6082 BstYI r'GATC_y Cuts at: 1972 2136 2244 2283 2852 3016 3412 3779 4164 Size: 164 108 39 569 164 396 367 385 Cuts at: 4164 4181 5633 5719 5731 6077 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 569 396 385 367 346 164 164 108 86 39 17 12 Bsu36I CC'TnA_GG Cuts at: 3129 3129 Size: 6082 BtgI C'CryG_G Cuts at: 513 1065 2004 2129 2667 3150 513 Size: 552 939 125 538 483 3445 Fragments arranged by size: 3445 939 552 538 483 125 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5343 Fragments arranged by size: 5343 683 56 BtsI GCAGTG_nn' Cuts at: 2160 2334 2476 2482 2600 3598 4392 4412 2160 Size: 174 142 6 118 998 794 20 3830 Fragments arranged by size: 3830 998 794 174 142 118 20 6 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6082 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6047 DraI TTT'AAA Cuts at: 3386 3386 Size: 6082 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5089 809 Size: 507 3773 1802 Fragments arranged by size: 3773 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2316 2401 3529 Size: 54 1425 50 275 18 486 85 1128 Cuts at: 3529 4418 6047 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1128 889 486 275 85 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3529 1537 Size: 275 1717 4090 Fragments arranged by size: 4090 1717 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4012 1353 Size: 2659 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4669 5043 5189 387 Size: 1064 138 3080 374 146 1280 Fragments arranged by size: 3080 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2079 2192 2543 2783 4145 4742 5529 Size: 393 447 113 351 240 1362 597 787 Cuts at: 5529 5797 1239 Size: 268 1524 Fragments arranged by size: 1524 1362 787 597 447 393 351 268 240 113 EcoO109I rG'GnC_Cy Cuts at: 2047 2212 3038 3134 3135 3191 3209 3227 3228 Size: 165 826 96 1 56 18 18 1 Cuts at: 3228 2047 Size: 4901 Fragments arranged by size: 4901 826 165 96 56 18 18 1 1 EcoRI G'AATT_C Cuts at: 3391 3769 3391 Size: 378 5704 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2461 2461 Size: 6082 FspI TGC'GCA Cuts at: 4588 4588 Size: 6082 HaeII r_GCGC'y Cuts at: 1678 3271 5229 1678 Size: 1593 1958 2531 Fragments arranged by size: 2531 1958 1593 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2618 2650 2775 2807 4723 Size: 32 135 32 1046 32 125 32 1916 Cuts at: 4723 4755 4797 4829 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1916 1046 135 125 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3426 3672 669 Size: 988 281 1488 246 3079 Fragments arranged by size: 3079 1488 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6082 HpaI GTT'AAC Cuts at: 3672 3672 Size: 6082 KpnI G_GTAC'C Cuts at: 3102 3407 3102 Size: 305 5777 MfeI C'AATT_G Cuts at: 3681 3681 Size: 6082 MluI A'CGCG_T Cuts at: 5958 5958 Size: 6082 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5196 5380 1305 Size: 3891 184 2007 Fragments arranged by size: 3891 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2318 2403 10 Size: 54 1425 343 486 85 3689 Fragments arranged by size: 3689 1425 486 343 85 54 MslI CAynn'nnrTG Cuts at: 518 1166 2201 2339 2949 4040 4399 4558 518 Size: 648 1035 138 610 1091 359 159 2042 Fragments arranged by size: 2042 1091 1035 648 610 359 159 138 MspA1I CmG'CkG Cuts at: 1606 2027 2834 3219 3498 4175 5323 5568 1606 Size: 421 807 385 279 677 1148 245 2120 Fragments arranged by size: 2120 1148 807 677 421 385 279 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2129 2667 3150 513 Size: 552 939 125 538 483 3445 Fragments arranged by size: 3445 939 552 538 483 125 NdeI CA'TA_TG Cuts at: 387 2948 387 Size: 2561 3521 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6082 NotI GC'GGCC_GC Cuts at: 3529 3529 Size: 6082 NspI r_CATG'y Cuts at: 1132 1229 2564 3440 4985 1132 Size: 97 1335 876 1545 2229 Fragments arranged by size: 2229 1545 1335 876 97 PasI CC'CwG_GG Cuts at: 1348 3355 1348 Size: 2007 4075 PciI A'CATG_T Cuts at: 4981 4981 Size: 6082 PflMI CCAn_nnn'nTGG Cuts at: 1108 2509 1108 Size: 1401 4681 PfoI T'CCnGG_A Cuts at: 2430 2430 Size: 6082 PmeI GTTT'AAAC Cuts at: 3386 3386 Size: 6082 PmlI CAC'GTG Cuts at: 2314 2314 Size: 6082 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4151 4183 5664 5696 4151 Size: 32 1481 32 4537 Fragments arranged by size: 4537 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2047 2212 3038 3191 2047 Size: 165 826 153 4938 Fragments arranged by size: 4938 826 165 153 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6082 PsiI TTA'TAA Cuts at: 3652 3652 Size: 6082 PspOMI G'GGCC_C Cuts at: 3134 3227 3134 Size: 93 5989 PstI C_TGCA'G Cuts at: 830 2026 2032 2167 3434 830 Size: 1196 6 135 1267 3478 Fragments arranged by size: 3478 1267 1196 135 6 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6082 PvuII CAG'CTG Cuts at: 2027 2834 3219 2027 Size: 807 385 4890 Fragments arranged by size: 4890 807 385 SalI G'TCGA_C Cuts at: 1936 3424 1936 Size: 1488 4594 SbfI CC_TGCA'GG Cuts at: 3434 3434 Size: 6082 ScaI AGT'ACT Cuts at: 4330 6042 4330 Size: 1712 4370 SexAI A'CCwGG_T Cuts at: 2969 2969 Size: 6082 SfcI C'TryA_G Cuts at: 826 1045 2022 2028 2096 2163 2835 3430 4565 Size: 219 977 6 68 67 672 595 1135 Cuts at: 4565 5246 5437 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 977 681 672 595 219 191 68 67 6 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6082 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6082 SmaI CCC'GGG Cuts at: 1872 3409 1872 Size: 1537 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2765 3140 4186 5087 5349 820 Size: 197 926 822 375 1046 901 262 1553 Fragments arranged by size: 1553 1046 926 901 822 375 262 197 SnaBI TAC'GTA Cuts at: 493 3873 493 Size: 3380 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6082 SphI G_CATG'C Cuts at: 1132 2564 3440 1132 Size: 1432 876 3774 Fragments arranged by size: 3774 1432 876 SspI AAT'ATT Cuts at: 5 52 4006 5 Size: 47 3954 2081 Fragments arranged by size: 3954 2081 47 StuI AGG'CCT Cuts at: 1517 2444 1517 Size: 927 5155 StyI C'CwwG_G Cuts at: 513 1065 2004 2129 2319 2667 3150 3178 3521 Size: 552 939 125 190 348 483 28 343 Cuts at: 3521 513 Size: 3074 Fragments arranged by size: 3074 939 552 483 348 343 190 125 28 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2878 3081 3125 4109 4126 4279 4464 Size: 1255 1298 203 44 984 17 153 185 Cuts at: 4464 4909 325 Size: 445 1498 Fragments arranged by size: 1498 1298 1255 984 445 203 185 153 44 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4328 6040 96 Size: 274 80 33 51 1443 2351 1712 138 Fragments arranged by size: 2351 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2137 2169 2886 2918 6042 6074 2137 Size: 32 717 32 3124 32 2145 Fragments arranged by size: 3124 2145 717 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2045 2971 1316 Size: 729 926 4427 Fragments arranged by size: 4427 926 729 XbaI T'CTAG_A Cuts at: 2086 3418 2086 Size: 1332 4750 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2326 2326 Size: 6082 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6082 XmaI C'CCGG_G Cuts at: 1870 3407 1870 Size: 1537 4545 XmnI GAAnn'nnTTC Cuts at: 4211 4211 Size: 6082 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3890 276 Size: 53 83 186 936 2356 2468 Fragments arranged by size: 2468 2356 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI