Restriction map for
pFN21AB5848
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# NO CUT ENZYMES ( 127 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspHI AspI
AvaI AviII AvrII BamHI BanI
BbeI BbrPI BbvCI BfrI BglII
BlnI BsaAI BsaHI BsaI BsaWI
BseAI BsiEI BsiHKAI BsiWI BsmAI
BsmBI BsmFI BsoBI BspDI BspEI
BspHI BspLU11I BsrBI BssHII BssSI
BstBI BstEII BstPI BstUI BstXI
ClaI DraI DraII DraIII DrdI
DsaI EagI Eam1105I EclXI Eco52I
EcoNI EcoO109I EcoO65I EcoT14I FseI
FspI HgaI Hin1I HincII HindII
HpaI KasI KpnI KspI MfeI
MluI MroI MunI MvnI NarI
NcoI NdeI NlaIV NotI NruI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RcaI SacI SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SmaI SnaBI SpeI SphI Sse8387I
StyI SwaI Tth111I Van91I XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 62 enzymes) #
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AocI 699 CCTCAGG CC^T[ACGT]AGG
Aor51HI 107 AGCGCT AGC^GCT
Asp700I 1132 GAATATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 84 GGTCC G^G[AT]CC
BalI 952 TGGCCA TGG^CCA
BbsI 621 GAAGAC GAAGAC(2/6)
BcgI 1280 GCAAGCTTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1078 GTATCC GTATCC(6/5)
BcnI 74 CCCGG CC^[GC]GG
BglI 40 GCCATCTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 868 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 868 GCTCAGC GC^T[ACGT]AGC
BpuAI 621 GAAGAC GAAGAC(2/6)
BsaBI 424 GATTTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 667 GCATTC GAATGC(1/-1)
BspMI 961 ACCTGC ACCTGC(4/8)
BsrFI 18 GCCGGC [GA]^CCGG[CT]
BstAPI 159 GCACCCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 441 GGATG GGATG(2/0)
BstYI 597 AGATCC [GA]^GATC[CT]
Bsu36I 699 CCTCAGG CC^T[ACGT]AGG
CelII 868 GCTCAGC GC^T[ACGT]AGC
Cfr10I 18 GCCGGC [GA]^CCGG[CT]
Cfr13I 84 GGTCC G^G[ACGT]CC
CpoI 83 CGGTCCG CG^G[AT]CCG
EaeI 952 TGGCCA [CT]^GGCC[GA]
Eco47III 107 AGCGCT AGC^GCT
Eco81I 699 CCTCAGG CC^T[ACGT]AGG
EcoRI 490 GAATTC G^AATTC
EcoRV 1065 GATATC GAT^ATC
EcoT22I 835 ATGCAT ATGCA^T
FokI 441 GGATG GGATG(9/13)
HindIII 1282 AAGCTT A^AGCTT
MaeIII 611 GTGAC ^GT[ACGT]AC
MamI 424 GATTTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 597 AGATCC [GA]^GATC[CT]
MluNI 952 TGGCCA TGG^CCA
MscI 952 TGGCCA TGG^CCA
MslI 724 CATTTCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 163 CCGCTG C[AC]G^C[GT]G
NaeI 18 GCCGGC GCC^GGC
NciI 74 CCCGG CC^[GC]GG
NgoMIV 18 GCCGGC G^CCGGC
NheI 873 GCTAGC G^CTAGC
NsiI 835 ATGCAT ATGCA^T
NspI 1424 ACATGC [GA]CATG^[CT]
PleI 256 GACTC GAGTC(4/5)
PstI 963 CTGCAG CTGCA^G
RsrII 83 CGGTCCG CG^G[AT]CCG
Sau96I 84 GGTCC G^G[ACGT]CC
SfaNI 181 GATGC GCATC(5/9)
SfcI 963 CTGCAG C^T[GA][CT]AG
SgrAI 17 CGCCGGCG C[GA]^CCGG[CT]G
SspI 753 AATATT AAT^ATT
StuI 458 AGGCCT AGG^CCT
TaqI 1289 TCGA T^CGA
Tsp45I 611 GTGAC ^GT[GC]AC
TthHB8I 1289 TCGA T^CGA
XbaI 1398 TCTAGA T^CTAGA
XcmI 1229 CCATTGTAGCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 597 AGATCC [GA]^GATC[CT]
XmnI 1132 GAATATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
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AccI 236 GTATAC GT^[AC][GT]AC
1243 GTATAC
AlwI 430 GATCC GGATC(4/5)
598 GATCC
AlwNI 571 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
892 CAGTTCCTG
BanII 117 GGGCTC G[GA]GC[CT]^C
513 GAGCCC
BmyI 117 GGGCTC G[AGT]GC[ACT]^C
513 GAGCCC
BpmI 577 CTGGAG CTGGAG(16/14)
898 CTGGAG
BseRI 1153 GAGGAG GAGGAG(10/8)
1375 GAGGAG
BsgI 157 CTGCAC GTGCAG(16/14)
631 CTGCAC
BsiYI 16 CCGCCGGCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
75 CCGGAAATCGG
BslI 16 CCGCCGGCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
75 CCGGAAATCGG
Bsp1286I 117 GGGCTC G[AGT]GC[ACT]^C
513 GAGCCC
Bsp1407I 888 TGTACA T^GTACA
1196 TGTACA
BsrDI 534 CATTGC GCAATG(2/0)
782 CATTGC
BsrGI 888 TGTACA T^GTACA
1196 TGTACA
Bst1107I 236 GTATAC GTA^TAC
1243 GTATAC
BstZ17I 236 GTATAC GTA^TAC
1243 GTATAC
FauI 162 CCCGC CCCGC(4/6)
22 GCGGG
HaeII 34 AGCGCC [GA]GCGC^[CT]
107 AGCGCT
HapII 19 CCGG C^CGG
75 CCGG
HpaII 19 CCGG C^CGG
75 CCGG
HphI 978 GGTGA GGTGA(8/7)
71 TCACC
MaeII 609 ACGT A^CGT
1350 ACGT
MspI 19 CCGG C^CGG
75 CCGG
SapI 273 GAAGAGC GCTCTTC(1/4)
1045 GAAGAGC
SmlI 454 CTTGAG C^T[CT][GA]AG
498 CTTGAG
SspBI 888 TGTACA T^GTACA
1196 TGTACA
TfiI 349 GAATC G^A[AT]TC
1221 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
BbvI BclI BfaI BsaJI BsrI
BssKI BstNI Cac8I CfoI DdeI
DpnII EarI EcoRII FbaI Fnu4HI
HaeIII HhaI HinP1I HinfI ItaI
Ksp632I MaeI MboI MboII MnlI
MseI MvaI MwoI NdeII NlaIII
RsaI Sau3AI ScrFI Tru9I TseI
Tsp509I TspRI