(Circular) (Six-base) MAPSORT of: pFN21AB5848.seq Check: 6804 from: 1 to: 6163 pFN21AB5848 6163 bp With 182 enzymes: SgfI * April 15, 2010 09:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3973 278 Size: 53 83 186 936 2437 2468 Fragments arranged by size: 2468 2437 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2242 3249 3506 1937 Size: 305 1007 257 4594 Fragments arranged by size: 4594 1007 305 257 Acc65I G'GTAC_C Cuts at: 3484 3484 Size: 6163 AclI AA'CG_TT Cuts at: 1563 4290 4663 1563 Size: 2727 373 3063 Fragments arranged by size: 3063 2727 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2645 4226 5610 5878 1632 Size: 1013 1581 1384 268 1917 Fragments arranged by size: 1917 1581 1384 1013 268 AfeI AGC'GCT Cuts at: 2114 2114 Size: 6163 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5966 AflIII A'CryG_T Cuts at: 1203 5062 6039 1203 Size: 3859 977 1327 Fragments arranged by size: 3859 1327 977 AgeI A'CCGG_T Cuts at: 4994 4994 Size: 6163 AhdI GACnn_n'nnGTC Cuts at: 4892 4892 Size: 6163 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6163 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6163 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2604 2636 2604 Size: 32 6131 AlwNI CAG_nnn'CTG Cuts at: 2581 2902 3906 5478 2581 Size: 321 1004 1572 3266 Fragments arranged by size: 3266 1572 1004 321 ApaLI G'TGCA_C Cuts at: 4220 5376 4220 Size: 1156 5007 ApoI r'AATT_y Cuts at: 1493 2495 2564 2643 2654 2951 3174 3207 3472 Size: 1002 69 79 11 297 223 33 265 Cuts at: 3472 3698 3850 5959 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 1002 297 265 226 223 152 79 69 33 11 AseI AT'TA_AT Cuts at: 160 1032 4717 160 Size: 872 3685 1606 Fragments arranged by size: 3685 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6163 AvaI C'yCGr_G Cuts at: 1870 1943 3488 1870 Size: 73 1545 4545 Fragments arranged by size: 4545 1545 73 BamHI G'GATC_C Cuts at: 3493 3860 3493 Size: 367 5796 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3484 4939 618 Size: 325 217 627 1697 1455 1842 Fragments arranged by size: 1842 1697 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2126 2522 3482 1391 Size: 735 396 960 4072 Fragments arranged by size: 4072 960 735 396 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2633 928 Size: 971 734 4458 Fragments arranged by size: 4458 971 734 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3274 3308 4354 4388 1903 Size: 34 1337 34 1046 34 3678 Fragments arranged by size: 3678 1337 1046 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3094 4055 5265 3094 Size: 961 1210 3992 Fragments arranged by size: 3992 1210 961 BclI T'GATC_A Cuts at: 1548 2382 2557 2934 1548 Size: 834 175 377 4777 Fragments arranged by size: 4777 834 377 175 BfrBI ATG'CAT Cuts at: 2842 2842 Size: 6163 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2051 4774 136 Size: 107 122 71 631 605 379 2723 1525 Fragments arranged by size: 2723 1525 631 605 379 122 107 71 BglII A'GATC_T Cuts at: 6158 6158 Size: 6163 BlpI GC'TnA_GC Cuts at: 2874 3580 2874 Size: 706 5457 Bme1580I G_kGCm'C Cuts at: 1790 4224 5380 1790 Size: 2434 1156 2573 Fragments arranged by size: 2573 2434 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6163 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3894 4852 442 Size: 416 53 83 399 24 2477 958 1753 Fragments arranged by size: 2477 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2882 1056 Size: 1826 4337 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2603 2924 4823 1239 Size: 1364 321 1899 2579 Fragments arranged by size: 2579 1899 1364 321 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2480 2524 4288 5153 5451 2480 Size: 44 1764 865 298 3192 Fragments arranged by size: 3192 1764 865 298 44 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4826 882 Size: 617 3327 2219 Fragments arranged by size: 3327 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3954 493 Size: 711 2750 2702 Fragments arranged by size: 2750 2702 711 BsaBI GATnn'nnATC Cuts at: 2433 6157 2433 Size: 3724 2439 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3970 4352 4987 275 Size: 53 83 186 936 2437 382 635 1451 Fragments arranged by size: 2437 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4596 4994 5268 5415 5826 4596 Size: 398 274 147 411 4933 Fragments arranged by size: 4933 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2395 2425 2562 2592 1178 Size: 30 165 30 992 30 137 30 4749 Fragments arranged by size: 4749 992 165 137 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3173 3395 1186 Size: 1987 222 3954 Fragments arranged by size: 3954 1987 222 BseYI C'CCAG_C Cuts at: 2175 5366 5862 2175 Size: 3191 496 2476 Fragments arranged by size: 3191 2476 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2147 2621 1163 Size: 573 411 474 4705 Fragments arranged by size: 4705 573 474 411 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3613 4374 5402 1540 Size: 275 186 1612 761 1028 2301 Fragments arranged by size: 2301 1612 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4224 4309 5380 4224 Size: 85 1071 5007 Fragments arranged by size: 5007 1071 85 BsmI GAATG_Cn' Cuts at: 2672 3674 3767 2672 Size: 1002 93 5068 Fragments arranged by size: 5068 1002 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2126 2522 3482 4224 4309 5380 1391 Size: 399 336 396 960 742 85 1071 2174 Fragments arranged by size: 2174 1071 960 742 399 396 336 85 BspEI T'CCGG_A Cuts at: 5826 5826 Size: 6163 BspHI T'CATG_A Cuts at: 4052 5782 4052 Size: 1730 4433 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2975 3518 844 Size: 2131 543 3489 Fragments arranged by size: 3489 2131 543 BsrBI CCG'CTC Cuts at: 1394 1823 4050 1394 Size: 429 2227 3507 Fragments arranged by size: 3507 2227 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2538 2786 4658 4832 65 Size: 1183 1290 248 1872 174 1396 Fragments arranged by size: 1872 1396 1290 1183 248 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2023 4807 4994 1700 Size: 110 213 2784 187 2869 Fragments arranged by size: 2869 2784 213 187 110 BsrGI T'GTAC_A Cuts at: 96 2893 3201 96 Size: 2797 308 3058 Fragments arranged by size: 3058 2797 308 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6163 BssSI C'ACGA_G Cuts at: 4223 5235 4223 Size: 1012 5151 BstAPI GCAn_nnn'nTGC Cuts at: 794 2170 794 Size: 1376 4787 BstBI TT'CG_AA Cuts at: 4967 4967 Size: 6163 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6163 BstYI r'GATC_y Cuts at: 1972 2602 3493 3860 4245 4262 5714 5800 5812 Size: 630 891 367 385 17 1452 86 12 Cuts at: 5812 6158 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 891 630 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2243 3250 2243 Size: 1007 5156 Bsu36I CC'TnA_GG Cuts at: 2705 2705 Size: 6163 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5611 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5424 Fragments arranged by size: 5424 683 56 BtsI GCAGTG_nn' Cuts at: 2240 3679 4473 4493 2240 Size: 1439 794 20 3910 Fragments arranged by size: 3910 1439 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6163 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6128 DraI TTT'AAA Cuts at: 3467 3467 Size: 6163 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5170 809 Size: 507 3854 1802 Fragments arranged by size: 3854 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2957 3610 4499 Size: 54 1425 50 275 18 1127 653 889 Cuts at: 4499 6128 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1127 889 653 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3610 1537 Size: 275 1798 4090 Fragments arranged by size: 4090 1798 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2273 2436 3045 4093 1353 Size: 920 163 609 1048 3423 Fragments arranged by size: 3423 1048 920 609 163 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4750 5124 5270 387 Size: 1064 138 3161 374 146 1280 Fragments arranged by size: 3161 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2603 2645 2924 4226 4823 5610 5878 Size: 393 971 42 279 1302 597 787 268 Cuts at: 5878 1239 Size: 1524 Fragments arranged by size: 1524 1302 971 787 597 393 279 268 42 EcoRI G'AATT_C Cuts at: 2495 3472 3850 2495 Size: 977 378 4808 Fragments arranged by size: 4808 977 378 EcoRV GAT'ATC Cuts at: 3072 3072 Size: 6163 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3074 3074 Size: 6163 FspI TGC'GCA Cuts at: 4669 4669 Size: 6163 HaeII r_GCGC'y Cuts at: 1678 2043 2116 5310 1678 Size: 365 73 3194 2531 Fragments arranged by size: 3194 2531 365 73 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2661 2693 4804 4836 4878 Size: 32 135 32 1089 32 2111 32 42 Cuts at: 4878 4910 1373 Size: 32 2626 Fragments arranged by size: 2626 2111 1089 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3507 3753 669 Size: 988 281 1569 246 3079 Fragments arranged by size: 3079 1569 988 281 246 HindIII A'AGCT_T Cuts at: 748 3287 748 Size: 2539 3624 HpaI GTT'AAC Cuts at: 3753 3753 Size: 6163 KpnI G_GTAC'C Cuts at: 3488 3488 Size: 6163 MfeI C'AATT_G Cuts at: 3762 3762 Size: 6163 MluI A'CGCG_T Cuts at: 6039 6039 Size: 6163 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3254 5277 5461 1305 Size: 1949 2023 184 2007 Fragments arranged by size: 2023 2007 1949 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2959 10 Size: 54 1425 343 1127 3214 Fragments arranged by size: 3214 1425 1127 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2733 4121 4480 4639 518 Size: 648 1567 1388 359 159 2042 Fragments arranged by size: 2042 1567 1388 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2170 3579 4256 5404 5649 1606 Size: 564 1409 677 1148 245 2120 Fragments arranged by size: 2120 1409 1148 677 564 245 NaeI GCC'GGC Cuts at: 2025 2025 Size: 6163 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5611 NdeI CA'TA_TG Cuts at: 387 387 Size: 6163 NgoMIV G'CCGG_C Cuts at: 2023 2023 Size: 6163 NheI G'CTAG_C Cuts at: 1052 2878 1052 Size: 1826 4337 NotI GC'GGCC_GC Cuts at: 3610 3610 Size: 6163 NsiI A_TGCA'T Cuts at: 2844 2844 Size: 6163 NspI r_CATG'y Cuts at: 1132 1229 3433 3521 5066 1132 Size: 97 2204 88 1545 2229 Fragments arranged by size: 2229 2204 1545 97 88 PasI CC'CwG_GG Cuts at: 1348 2119 1348 Size: 771 5392 PciI A'CATG_T Cuts at: 5062 5062 Size: 6163 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6163 PfoI T'CCnGG_A Cuts at: 2580 2901 2580 Size: 321 5842 PmeI GTTT'AAAC Cuts at: 3467 3467 Size: 6163 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4232 4264 5745 5777 4232 Size: 32 1481 32 4618 Fragments arranged by size: 4618 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6163 PsiI TTA'TAA Cuts at: 3733 3733 Size: 6163 PstI C_TGCA'G Cuts at: 830 2972 3515 830 Size: 2142 543 3478 Fragments arranged by size: 3478 2142 543 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6163 RsrII CG'GwC_CG Cuts at: 2089 2089 Size: 6163 SalI G'TCGA_C Cuts at: 1936 3505 1936 Size: 1569 4594 SapI GCTCTTCn'nnn_ Cuts at: 2273 3045 2273 Size: 772 5391 SbfI CC_TGCA'GG Cuts at: 3515 3515 Size: 6163 ScaI AGT'ACT Cuts at: 4411 6123 4411 Size: 1712 4451 SfcI C'TryA_G Cuts at: 826 1045 2968 3511 4646 5327 5518 826 Size: 219 1923 543 1135 681 191 1471 Fragments arranged by size: 1923 1471 1135 681 543 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6163 SgrAI Cr'CCGG_yG Cuts at: 1700 2023 1700 Size: 323 5840 SmaI CCC'GGG Cuts at: 1872 3490 1872 Size: 1618 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2459 2503 4267 5168 5430 820 Size: 197 926 516 44 1764 901 262 1553 Fragments arranged by size: 1764 1553 926 901 516 262 197 44 SnaBI TAC'GTA Cuts at: 493 3954 493 Size: 3461 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6163 SphI G_CATG'C Cuts at: 1132 3521 1132 Size: 2389 3774 SspI AAT'ATT Cuts at: 5 52 2760 4087 5 Size: 47 2708 1327 2081 Fragments arranged by size: 2708 2081 1327 47 StuI AGG'CCT Cuts at: 1517 2465 1517 Size: 948 5215 StyI C'CwwG_G Cuts at: 513 1065 3602 513 Size: 552 2537 3074 Fragments arranged by size: 3074 2537 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2077 4190 4207 4360 4545 4990 325 Size: 1255 497 2113 17 153 185 445 1498 Fragments arranged by size: 2113 1498 1255 497 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2893 3120 3201 Size: 274 80 33 51 1443 916 227 81 Cuts at: 3201 4409 6121 96 Size: 1208 1712 138 Fragments arranged by size: 1712 1443 1208 916 274 227 138 81 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6123 6155 6123 Size: 32 6131 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6163 XbaI T'CTAG_A Cuts at: 3403 3499 3403 Size: 96 6067 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3241 3241 Size: 6163 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6163 XmaI C'CCGG_G Cuts at: 1870 3488 1870 Size: 1618 4545 XmnI GAAnn'nnTTC Cuts at: 3141 4292 3141 Size: 1151 5012 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3971 276 Size: 53 83 186 936 2437 2468 Fragments arranged by size: 2468 2437 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI RsrII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI DraIII EcoICRI EcoNI EcoO109I FseI FspAI KasI NarI NruI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI