Restriction map for
pFN21AB5842
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# NO CUT ENZYMES ( 152 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI AlwNI AocI Aor51HI ApaI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaII AviII AvrII
BalI BamHI BanII BbeI BbsI
BbvCI BciVI BcnI BfrI BglI
BglII BlnI BpuAI BsaBI BsaHI
BsaWI BseAI BsiEI BsiWI BsmBI
BsmFI BspDI BspEI BspLU11I BspMI
BsrFI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I Cfr10I ClaI CpoI DrdI
DsaI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoO65I EcoT14I
EcoT22I FauI FseI FspI HapII
HgaI Hin1I HincII HindII HindIII
HpaI HpaII KasI KpnI Ksp632I
KspI MamI MfeI MluI MluNI
MroI MscI MslI MspI MunI
MvnI NaeI NarI NciI NcoI
NgoMIV NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmeI PpuMI PshAI
PshBI Psp1406I PstI PvuI RsrII
SacI SacII SalI SapI SbfI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspI StuI StyI SwaI Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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Alw44I 646 GTGCAC G^TGCAC
ApaLI 646 GTGCAC G^TGCAC
AvaI 1184 CCCGAG C^[CT]CG[GA]G
BanI 442 GGTGCC G^G[CT][GA]CC
BbrPI 649 CACGTG CAC^GTG
BcgI 1107 GCAGGAACATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 1295 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 1295 GCTTAGC GC^T[ACGT]AGC
BsaAI 649 CACGTG [CT]AC^GT[GA]
BsaI 1246 GAGACC GGTCTC(1/5)
BsgI 769 GTGCAG GTGCAG(16/14)
BsmAI 1246 GAGAC GTCTC(1/5)
BsmI 377 GCATTC GAATGC(1/-1)
BsoBI 1184 CCCGAG C^[CT]CG[GA]G
Bsp1407I 849 TGTACA T^GTACA
BsrBI 438 GAGCGG CCGCTC(-3/-3)
BsrGI 849 TGTACA T^GTACA
CelII 1295 GCTTAGC GC^T[ACGT]AGC
CfoI 954 GCGC GCG^C
DraI 225 TTTAAA TTT^AAA
DraII 66 AGGCCCT [GA]G^G[ACGT]CC[CT]
DraIII 649 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 8 CGGCCA [CT]^GGCC[GA]
EcoO109I 66 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRI 864 GAATTC G^AATTC
EcoRV 403 GATATC GAT^ATC
HaeII 953 AGCGCC [GA]GCGC^[CT]
HhaI 954 GCGC GCG^C
HinP1I 954 GCGC G^CGC
MaeII 650 ACGT A^CGT
MwoI 1148 GCTTATGCCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NheI 1047 GCTAGC G^CTAGC
PmaCI 649 CACGTG CAC^GTG
PmlI 649 CACGTG CAC^GTG
ScaI 932 AGTACT AGT^ACT
SexAI 201 ACCTGGT A^CC[AT]GGT
SfaNI 551 GCATC GCATC(5/9)
SspBI 849 TGTACA T^GTACA
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AlwI 243 GGATC GGATC(4/5)
681 GGATC
AspHI 3 GAGCAC G[AT]GC[AT]^C
646 GTGCAC
BbvI 168 GCAGC GCAGC(8/12)
1353 GCAGC
BclI 711 TGATCA T^GATCA
1310 TGATCA
BpmI 987 CTCCAG CTGGAG(16/14)
92 CTGGAG
BsiHKAI 3 GAGCAC G[AT]GC[AT]^C
646 GTGCAC
BspHI 860 TCATGA T^CATGA
1131 TCATGA
BsrDI 384 CATTGC GCAATG(2/0)
499 GCAATG
BstXI 581 CCAAAGTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1257 CCATCGTATTGG
BstYI 243 GGATCT [GA]^GATC[CT]
681 GGATCT
FbaI 711 TGATCA T^GATCA
1310 TGATCA
HinfI 251 GAATC G^A[ACGT]TC
674 GAATC
MflI 243 GGATCT [GA]^GATC[CT]
681 GGATCT
NdeI 941 CATATG CA^TATG
1316 CATATG
RcaI 860 TCATGA T^CATGA
1131 TCATGA
SfcI 532 CTACAG C^T[GA][CT]AG
1430 CTGTAG
TaqI 1009 TCGA T^CGA
1116 TCGA
TfiI 251 GAATC G^A[AT]TC
674 GAATC
TseI 168 GCAGC G^C[AT]GC
1353 GCAGC
Tsp45I 268 GTGAC ^GT[GC]AC
756 GTCAC
TthHB8I 1009 TCGA T^CGA
1116 TCGA
XhoII 243 GGATCT [GA]^GATC[CT]
681 GGATCT
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# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
BfaI BmyI BsaJI BseRI BsiYI
BslI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
EcoNI EcoRII Fnu4HI FokI HaeIII
HphI ItaI MaeI MaeIII MboI
MboII MnlI MseI MspA1I MvaI
NdeII NlaIII NlaIV PvuII RsaI
Sau3AI Sau96I ScrFI Tru9I Tsp509I
TspRI